Search PubMed⌕ Search

Biomedical subjects

O Barta

Publications and source records attributed to O Barta.

At least 55 records · Page 3Linked to original sources
PubMed1980

Hemolytic assay for goat (caprine) and swine (porcine) complement.

Optimal conditions for assaying hemolytic complement of goat (caprine) and swine (porcine) sera were determined. Effects of the following were tested: pH, ionic strength, calcium and magnesium ion concentrations, time and temperature of incubation, and ethylenediamine tetracetate concentration. Guinea pig erythrocytes sensitized with goat or cattle antibodies were the most sensitive target cells for goat complement. Sheep and cattle erythrocytes sensitized with rabbit hemolysin were the best target cells for swine complement. Barbital buffer, pH 7.3, ionic strength of 90 nmM relative salt concentration, containing 0.5 mM CaCl2 and 1 mM MgCl2 was the best for swine complement assay. Goat complement lysed best in a barbital buffer, pH 8, ionic strength of 90 to 120 mM of relative salt concentration, in presence of 0.5 mM CaCl2 and 1 mM MgCl2. The optimal incubation temperature was 37 degrees C for both complements. The complement dependent lysis required 75 minutes to reach its maximum. Ethylenediamine tetracetate in 4 mM concentration completely inhibited lysis by both species complements. The CH50 for goat sera varied between 18 and 75 per ml, in swine sera between 75 and 210 per ml.

Animals↗
PubMed1980

[The effect of operations on soft tissues in the development of tarsal bones in patients with clubfoot (author's transl)].

Authors investigated the process of ossification of tarsal bones in patients with club foot previously treated with redression in plaster and soft tissue release. A control group of 150 children between 1 and 12 years of age without club foot deformity was used. Based on analysis of two side X-ray pictures it is stated that 17.8 per cent of cases with club foot reveal a general backwardness and other variables of ossification. No obvious damaging effect of the operation was observed. In their opinion by means of soft tissue release the noxious effect of forced manipulation can be prevented. By ceasing the malposition in time the ossification can be accelerated.

Child↗
PubMed1980

[Transient arthritis of the hip in childhood and Perthes disease (author's transl)].

The possibilities of the relationship between transient arthritis of the hip and Perthes disease have been analysed here. They pointed to the fact, that the intra-articular pressure is increased by the overproduction of the synovial fluid as a result of inflammation, and a continuously high pressure can lead to necrosis of the femoral of inflammation of the femoral capital epiphysis. Experiences are reported here, when an intertrochanteric osteotomy at the early stage of Perthes disease was performed with simultaneous fenestration of the joint capsule for decompression, and a great amount of synovial fluid was discharged under high pressure. They conclude therefore, that transient synovitis can not be regarded only as a harmless condition, but it can precede Perthes disease.

Arthritis, Rheumatoid↗
PubMed1980

[Results of soft tissue operations in the management of congenital club foot (author's transl)].

A review is given on the experiences of 152 cases of operatively treated club foot. Exact X-ray assessment is thought to be very important, as even after excellent morphological and functional end results residual X-ray changes have been observed which potentially may lead to the redevelopment of abnormalities. Clinical and radiological assessment together can only be informative of the result of management. Analysis of the "own" and "referred" patients showed better clinical and radiological results in the first group, which has been attributed to the early operation, regular follow-up and care.

Child↗
PubMed1980

[Measurement of intraarticular pressure in the hip of young rabbits (author's transl)].

The changes of intraarticular pressure in young rabbits have been examined, using a model developed earlier by the authors. The amount of pressure necessary to the expansion of the capsule and the lateral displacement of the femoral head has been established. Arteriographic studies have been added to these examinations and the pressure limits influencing the intraarticular blood supply have been measured. They showed that changes in the position of the hip influence significantly the amount of the intraarticular pressure. Some modifications in the management of Perthes-disease and transient arthritis of the hip are recommended on the basis of their experiments.

Animals↗
PubMed1978

Hemolytic assay of complement and its components from Syrian hamster (Mesocricetus auratus) and Mongolian gerbil (Mariones unguiculatus).

Syrian hamster sera may be assayed for hemolytic complement (C) activity by standard procedures with sensitized sheep erythrocytes as used for human sera, but mongolian gerbil sera had higher complement titers when tested with sensitized guinea pig erythrocytes as target cells. The optimal conditions for in vitro testing of hamster C were pH 7.3, ionic strength 0.15, and presence of 1 micrometer of Mg2+ and 0.3 micrometer Ca2+. For gerbil C the optimum pH was 8, ionic strength 0.074, and the same concentration of Mg2+ and Ca2+ as for hamster. Incubation at 37 degrees C for 60 minutes yielded optimal results. Complement components in the serum of both species could be tested with commercially prepared intermediates of sheep erythrocytes pretreated with guinea pig C1 and human C4 respectively, using purified human C2 through 9 and standard methods applied for testing human C components. Both species have all nine components of the complement system.

Animals↗
PubMed1978

Genetics of adolescent idiopathic scoliosis.

A genetic family study was undertaken by photofluorography of the first, second, and third degree relatives of 116 index patients with adolescent idiopathic scoliosis (AIS). The index patients were ascertained in the course of an epidemiological screening. The pattern of familial clusters and the recurrence risk related to the number of affected relatives and to the severity of the disorder in the index patients support the theory of polygenic inheritance, a multifactorial-threshold aetiological model. The recurrence risk table for first degree relative, prepared by computerised data processing and analysis, may contribute to the early diagnosis and prevention of the disorder.

Adolescent↗
PubMed1978

Testing of hemolytic complement components in domestic animals.

Total complement (C) and its components were assayed in the serum of 8 species of domestic animals, using commercially prepared cellular intermediates of sheep erythrocytes and functionally pure guinea pig and human components of the C system. Testing was done according to methods recommended by the producer for testing human C components. The late-acting components (C6 throug C9) and C1 were detected in carnivorous (dog and cat) and omnivorous (swine) animals. Undetectable or low titers of C4, C2, C3, and C5 were present in large herbivorous animals (cattle, horse, sheep, and goat), indicating major differences in comparison with human or guinea pig components of C. Porcine serum contained an inhibiting substance which interfered with testing C2 and later-acting components at serum dilutions up to 1:100. All components except C2 were detected in chicken serum. The binding or activation (or both) of C4, C2, C3, and C5 is more species specific than is the binding or activation (or both) of other components. Requirements for species specificity between antibody and C1 were not detected. Presence of C1 inactivator was detected in bovine, caprine, equine, and ovine sera. The CH50 (50% hemolysis) titers of C components tested in pooled serum samples from the 8 species of clinically healthy domestic animals are presented.

Animals↗
PubMed1977

[Early surgery of the soft tissues in congenital clubfoot].

The role and position of early complete operative procedures on the soft tissues in the treatment of the congenital club foot is discussed in the article. The authors report on the operative indications in principles and in their own practice. Closed and operative treatment are not contradictory but complementary terms. The aim of the operative treatment is to bring all residual contractures following conservative treatment to an end. It must be done by relatively minimal intervention on the soft tissues and as early as possible. Thus the proper development of the foot and the prevention of bone deformation can be attained.

Child↗