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Biomedical subjects

O Barta

Publications and source records attributed to O Barta.

At least 37 records · Page 2Linked to original sources
PubMed1984

Clinical and laboratory characterization of Basenjis with immunoproliferative small intestinal disease.

Eleven adult Basenji dogs with immunoproliferative small intestinal disease (IPSID) were studied. Two items of history related to the digestive tract were characteristic: (i) chronic intractable diarrhea in most dogs, and (ii) progressive emaciation. Anorexia was intermittent in only a few dogs. In addition, skin lesions of various degrees of severity were observed, including alopecia of pinnae and ventrum, hyperpigmentation and hyperkeratosis of pinnae, and necrosis and ulcerations of margins of pinnae. The cause of the skin lesions was not determined; however, hypothyroidism did not appear to contribute to the skin changes. Standard hematologic and serum chemical values were not consistently abnormal. However, a poorly regenerative anemia, mild neutrophilia, and increased aspartate aminotransferase and alanine aminotransferase activities were generally observed in severely affected dogs. The Pelger-Huet anomaly was identified in dog 3. Maldigestion and malabsorption as determined by the N-benzoyl-L-tyrosyl-p-aminobenzoic acid and d-xylose test was documented to varying degrees in dogs with IPSID. Maldigestion was correlated with functional pancreatic exocrine insufficiency. Severe malabsorption was documented in only 3 dogs. Serum gastrin values were evaluated in these dogs because of a prior observation of parietal cell hyperplasia and gastric ulceration. Hypergastrinemia was documented in 3 dogs. Additional studies will be necessary to determine whether an acid hypersecretory state contributes to the pathogenesis of IPSID in Basenjis.

4-Aminobenzoic Acid↗
PubMed1983

Serum hyperviscosity syndrome associated with Ehrlichia canis infection in a dog.

A 10-year-old male Lhasa Apso was examined because of pyrexia, vomiting, weight loss, lethargy, and disorientation. The clinical and laboratory findings were supportive of a diagnosis of serum hyperviscosity syndrome associated with an Ehrlichia canis infection. After tetracycline therapy the serum hyperviscosity syndrome and E. canis infection were successfully resolved.

Animals↗
PubMed1983

Lymphocyte transformation suppression caused by pyoderma--failure to demonstrate it in uncomplicated demodectic mange.

Three dogs with demodectic mange uncomplicated by a bacterial infection and 9 dogs with demodectic mange and pyoderma were tested for their lymphocyte response to phytomitogens in vitro and for the presence of the serum's lymphocyte immunoregulatory factors (SLIF) suppressing blastogenesis. None of the 3 dogs with uncomplicated demodectic mange showed any detectable dysfunction of their lymphocytes or presence of the blastogenesis suppressing SLIF. Their lymphocytes generally responded to the mitogens with more blastogenesis than lymphocytes from healthy controls. On the other hand, in the group of 9 dogs with demodicosis complicated by a bacterial infection, high levels of the blastogenesis suppressing SLIF for concanavalin A-sensitive cells were detected in 4 dogs, for phytohemagglutinin-sensitive cells in 2 dogs, and for pokeweed mitogen-sensitive cells in 1 (of only 3 tested) dog. Dysfunction of lymphocytes per se (detected by a decreased blastogenesis in nonsuppressive normal canine and bovine sera) was detected in 3 dogs with demodicosis with pyoderma. The success of the treatment of demodectic mange or the bacterial skin infection did not correlate with the previous presence or absence of the blastogenesis suppressing SLIF. The treatment of pyoderma was less successful in dogs with an increase in blastogenesis of unstimulated cells in fresh normal canine serum over that in autologous serum. All 3 dogs with a detected dysfunction of their lymphocytes either died or were euthanatized as untreatable cases. It is concluded that the development of demodectic mange per se did not cause the appearance of the blastogenesis suppressing SLIF, which was primarily related to the appearance and extent of the secondary bacterial skin infection.

Animals↗
PubMed1983

Serum proteins in healthy Basenjis and Basenjis with chronic diarrhea.

A serologic survey was conducted to characterize the electrophoretic patterns of serum proteins in healthy Basenji dogs (n = 137) and Basenjis with chronic diarrhea (n = 32). Serum protein electrophoresis values for Basenjis were similar to previously reported values from dogs of other breeds. Dogs with histologically confirmed lymphocytic-plasmacytic enteritis had a statistically significant (P less than 0.05) decrease in total protein, albumin, and albumin/globulin ratio. alpha 2-Globulin and gamma-globulin values were significantly increased in old dogs (9 years of age or older) with lymphocytic-plasmacytic enteritis. Although not statistically significant, gamma-globulin values were generally increased in Basenjis with lymphocytic-plasmacytic enteritis when compared with the values in age-matched clinically healthy Basenjis.

Alpha-Globulins↗
PubMed1983

Counterimmunoelectrophoresis (immunoelectroosmosis) and serum electrophoretic pattern in serologic diagnosis of canine blastomycosis.

Counterimmunoelectrophoresis (CIEP) with blastomyces and histoplasma antigens was used in a serologic study of 181 dogs clinically suspected of having blastomycosis and of 8 dogs with confirmed blastomycosis or histoplasmosis. Thirteen of the 181 dogs, positive by CIEP, were euthanatized, and the diagnosis was confirmed by cultivation and/or microscopic detection of Blastomyces dermatitidis. Additional CIEP-positive dogs were confirmed by staining of aspirates collected in vivo. Radiographic support for the diagnosis was reported in 4 other dogs in which histoplasmosis was excluded by a negative CIEP with histoplasma antigen. The precipitating antibody may disappear during the course of the disease, as it did in 1 dog treated with amphotericin B, but not cured. This dog reverted from CIEP-positive to CIEP-negative within 17 months of treatment (with a weak reaction after 10 months of treatment). The CIEP-detectable antibody was present only in 1 dog without a confirmation by histopathologic findings or cultivation among 24 well-documented cases and 181 total tested sera. The CIEP was more sensitive and specific than was the gel-diffusion precipitin test, eliminated the problems of anticomplementarity that often affected the results of complement-fixation tests with canine sera, and served well in detecting dogs with blastomycosis. Electrophoretic pattern of sera from CIEP-positive dogs with blastomycosis showed a decrease in albumin and an increase in alpha 2- and often in beta- and gamma-globulins, with a substantial decrease of the albumin/globulin ratio.

Animals↗
PubMed1983

Lymphocyte transformation and humoral immune factors in Basenji dogs with immunoproliferative small intestinal disease.

Serum protein concentrations and 4 immunologic factors were determined in 5 Basenji dogs with immunoproliferative small intestinal disease. There was no correlation between the total serum proteins, immunoglobulin G and immunoglobulin M concentrations, and physical health status of the animals. The severity of clinical signs correlated roughly with decrease in albumin and increase in globulin concentrations. The main changes were detected in beta- and fast gamma-globulins. The total hemolytic complement levels were decreased in the 2 most severely affected animals below the minimal laboratory values observed in healthy animals. Alteration in the intrinsic responsiveness of lymphocytes to various mitogens did not correlate with progression in severity of the disease. Correlation between the appearance of blastogenesis-suppressing substances in serum and the severity of the disease was only partial: Sera (at 20% concentration) from the 2 most severely affected dogs completely suppressed blastogenesis induced by all 3 mitogens. The sera of 3 other dogs either did not suppress or suppressed only concanavalin A-induced mitogenesis and to a lesser extent phytohemagglutinin-induced mitogenesis without correlations to the overall clinical status. The disturbances of immunologic mechanisms were detected after the appearance of clinical disease, were not considered the cause of immunoproliferative small intestinal disease, may represent a manifestation of the secondary infection, and may contribute to aggravation of the clinical course.

Animals↗
PubMed1982

Effect of serum on lymphocyte blastogenesis. I. Basic characteristics of action by diseased dog serum.

The immunoregulatory effect of serum on phytomitogen-induced lymphocyte blastogenesis was studied in 4 sera from diseased dogs and 1 serum from a clinically healthy dog. The results indicated that: (1) Each of the diseased animals responded to the given infection with a specific pattern of blastogenesis inhibition. (2) The blastogenesis suppression in vitro was proportional to the content of the suppressive serum in the medium. (3) A simultaneous presence of the mitogen and the suppressing "serum's lymphocyte immunoregulatory factors" (SLIF) was necessary for inducing blastogenesis suppression. (4) The suppressive sera most probably acted directly on the cells. (5) The final effect of the sera on lymphocyte blastogenesis was a result of an orchestrated action of blastogenesis-supporting, augmenting, and suppressing SLIF cooperating with the mitogen. (6) The suppressive pattern varied with the individual peripheral blood lymphocytes populations used in the test. (7) The blastogenesis-suppressing SLIF was heat-stable, noncytotoxic, and was not or only partially removable by absorption with peripheral blood lymphocytes. (8) The testing of SLIF activities required the use of various animal lymphocytes and a relatively complex setup of mitogens and control serum combinations for correct interpretations.

Animals↗
PubMed1982

Leptospiral and brucellar titers in frozen bovine sera after 20 years of storage.

Generally good agreement was obtained between the rankings of sera titers when originally tested (T1), and those of the same sera 20 years later (T2). At the later time, the majority of samples (88%) were in, or within 1 dilution factor of their classification at T1. Test results for Brucella abortus and Leptospira interrogans var pomona antibodies demonstrated that original titers, in the upper-lower limits of 20 to 50, had decreased over a wide range (20% to 90%), and the later tests show that the greater the original titer, the greater the proportional decrease. Thus, titers of 20,000 at T1 had decreased by 49% to 99% and those of 200,000, by 95% to 99.8%. Variations in T2 test results were noted between some samples which had given identical results at T1. A decrease in titer over time has been demonstrated. An estimate of the expected half-lives of sera of differing initial Leptospira antibody titers is that for each unit increase in log10 of the initial titer, the half-life is reduced by about 50%. The heterogeneity of the immune response is postulated as the reason for this variation.

Agglutination Tests↗
PubMed1981

Bullous pemphigoid refractory to recommended dosage of prednisolone in a dog.

Bullous pemphigoid in a dog was diagnosed on the basis of clinical, histopathologic, and immunologic criteria. There were widespread ulcerative lesions of the skin. Numerous ulcerative lesions and occasional bullous lesions were on the oral mucous membranes. Histopathologic changes included dermal-epidermal separation, with subepidermal production of bullae. Direct immunofluorescent staining of the skin revealed depositions of immunoglobulin G and of the 3rd component of the complement system. Indirect immunofluorescent staining of donor skin sections pretreated with patient's serum revealed antibasement membrane antibody in the patient's serum. Most of the data in the serum immune profile were within normal ranges. Prednisolone therapy did not effect remission at the dosage of 4.4 mg/kg/day, but led to the beginning of remission within 7 days at a dosage of 6.6 mg/kg/day. The dosage was gradually reduced to 1.1 mg/kg, at 30 days, after which the drug was given on alternate days at that dosage. The healing was complete within 35 days, without any remissions when the dog was last examined (ie, over 6 months).

Animals↗
PubMed1981

Hemolytic complement and its components in Syrian hamsters: a study of five strains uninfected and infected with Brugia pahangi.

Complement profiles were tested in outbred (LVG) Syrian hamsters (Mesocricetus auratus) and compared to the MHA, LHC, PD4, and CB inbred strains. The total C and C component concentrations in the sera varied among the strains and were in the following ranges in untreated animals (in CH50 units per ml): total C, 140-260 (undetectable in PD4 and CB); Cl, 14,000-25,000; C2, 200-800 (except PD 4 and CB); C3, 40,000 and 80,000; C4, 2,000-2,800 (except PD4 and CB); C4, 40,000-80,000; C6, 3,600-6,000 (undetectable in PD4 and CB); C7, 50,000-350,000; C8, 10,000-30,000; C9, 30,000-60,000. The PD4 and CB strains had undetectable total C and C6, and their exact C2 and C4 levels could not be determined, but were lower than in the other strains. The MHA strain had the highest total C levels, but had significantly lower (1/3 or less) C7 levels than the other strains of hamsters. Infection of hamsters with the filarid nematode Brugia pahangi for four to five months produced moderate decreases in the total C and C3 levels, but varied changes in other C components. Six infected and three uninfected animals died during the experiment from spontaneous enteritis and weight loss.

Animals↗
PubMed1981

Laboratory techniques of veterinary clinical immunology: a review.

Laboratory tests currently used or easily applicable in veterinary clinical immunology were reviewed in the following three categories: (1) tests detecting disorders in humoral immune response: serum protein screening tests, gammaglobulin estimation and complement testing; (2) tests detecting disorders in cellular immune response: phagocyte function tests and lymphocyte function tests; (3) tests detecting autoimmune disorders: detection of antinuclear antibodies, rheumatoid factor, autoimmune hemolytic diseases, and autoimmune organ and tissue disorders. The principles of the tests and the interpretations of the results are presented with the appropriate references.

Animal Diseases↗
PubMed1981

Lymphocyte transformation test in veterinary clinical immunology.

Lymphocyte transformation test is a powerful tool in laboratory testing of immunologic competence of animals. The impaired function of the lymphocytes or presence of mitogenesis suppressing factors in the patient serum were detected by comparing lymphocyte transformation (expressed as thymidine incorporation) obtained in media containing either autologous, homologous, or fetal calf serum additions. Most valuable results were obtained by using at least two, preferably three, different phytomitogens: concanavalin A (Con A), pokeweed mitogen (PWM), and phytohemagglutinin (PHA) at optimal concentrations (Con A, 15 micrograms/ml. PWM and PHA, 5 micrograms/ml) and decreased concentrations (Con A, 5 micrograms/ml, PWM and PHA, 1 microgram/ml). Mitogenesis induced by lipopolysaccharide was considerably smaller and not used routinely. With 2 X 10(5) lymphocytes/well, the background count of unstimulated lymphocytes in autologous serum in healthy dogs was usually between 100 and 400 counts/min (CPM), in clinically healthy cattle and horses from 200 to over 2000 CPM. Higher CPM were rarely detected without clinical disease. Increased background counts were often associated with viral infections, leukemias and lymphoreticular hyperplasias, decreased background counts were associated with various diseases. The stimulation indexes (SI) of healthy animals in autologous serum with Con A, (5 micrograms/ml) or PWM or PHA (1 microgram/ml) were in the range from 100 to 1000 in the dogs, in the tens for Con A and in hundreds for PWM and PHA in horses and cattle. Increased SI were present during the incubation period of various diseases. Decreased SI were associated with numerous infectious and lymphoreticular diseases and were caused by any of the following: (1) the presence of serum immunosuppressive factor(s) in the patient serum, (2) the decreased response of lymphocytes to mitogens, or (3) increased mitogenicity of lymphocytes due to unidentified serum factors in absence of phytomitogens.

Animal Diseases↗
PubMed1981

Feline (cat) hemolytic complement optimal testing conditions.

Optimal conditions for assaying feline (cat) hemolytic complement (C) were determined. Effects of the following factors on C titers were tested: pH, ionic strength; calcium, magnesium, and ethylenediamine tetraacetate concentrations; time and temperature of incubation. Guinea pig erythrocytes sensitized with feline antibody were the most sensitive target cells for feline C. A buffer of pH 7.3, with an ionic strength of 0.06 and containing 0.3 mM CaCl2 and 1 mM MgCl2, was optimal for feline C assay. A concentration of 4 mM ethylenediamine tetraacetate in calcium- and magnesium-free buffer inhibited the C reactivity to 1/53rd of the original titer. Sera from clinically healthy cats contained 70 to 150 CH50 (50% hemolytic) U/ml when tested, using 1 x 10(8) sensitized guinea pig erythrocytes/ml.

Animals↗
PubMed1980

Canine brucellosis: comparison of clinical manifestations with serologic test results.

Slide agglutination and mercaptoethanol tube agglutination tests for canine brucellosis were performed on 158 dogs. Clinical status was compared with the serologic test results. Sera were from 56 clinically normal dogs, 63 dogs with reproductive disorders, and 39 dogs with various nonreproductive disorders that could be associated with canine brucellosis. Ten of 21 (48%) aborting bitches and 2 of 9 (22%) bitches with other reproductive disorders were seropositive for brucellosis. Enlarged testicles, orchitis, and epididymitis were the main clinical disorders associated with positive (33%) or suspect (20%) serologic reactions in 15 male dogs. In 13 dogs of both sexes, diskospondylitis and osteomyelitis were the most common nonreproductive disorders associated with seropositive status for canine brucellosis (46%). Of 138 stray dogs, 17 were seropositive for canine brucellosis. Treatment of seropositive animals with antibiotics gave variable results. Of the 296 serum samples tested, 43 (14.5%) gave a positive reaction by the slide agglutination test but were negative by the mercaptoethanol tube agglutination test. Correlation was not found between serologic results and sex or breed.

Abortion, Veterinary↗