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Biomedical subjects

N Yamane

Publications and source records attributed to N Yamane.

At least 73 records · Page 4Linked to original sources
PubMed1989

Mitochondrial aspartate aminotransferase by immunoprecipitation method in patients with acute myocardial infarction.

The semiautomated kinetic procedure for determining cytoplasmic aspartate aminotransferase (c-AST) and mitochondrial aspartate aminotransferase (m-AST) activities was studied by the use of the immunoprecipitation method with anti-c-AST antibody in serum samples. The measured activity for m-AST remained constant after the addition of c-AST up to 1,000 IU/l throughout the 60-min incubation period. The measurements of m-AST activity were reproducible, selective and complete as determined by a purified m-AST. The precision of this method was as good as that of the manual method (CV 2.04%). The present method and the manual method gave approximately equal results for m-AST (r = 0.987). The effects of activations on m- and c-AST activity were compared by the addition of pyridoxal 5'-phosphate to sera of various diseases. A higher activation ratio by pyridoxal 5'-phosphate was observed on both aspartate aminotransferase activities in the serum of patients with ischemic heart diseases than in the serum of patients with liver diseases.

Adult↗
PubMed1988

Ectopic production of salivary-type amylase by a IgA-lambda-type multiple myeloma.

A patient with IgA-lambda-type multiple myeloma who appeared to be secreting salivary-type amylase ectopically is reported. Both the patient's serum and urine contained high levels of amylase. Amylase activity in the supernatant of cultured myeloma cells, obtained from the patient's pleural effusion while he had malignant pleuritis, increased almost linearly from the time of cell seeding. The presence of IgA and S-type amylase in the myeloma cells was demonstrated cytochemically and by immunoelectron microscopy. These observations showed that amylase was produced by these human myeloma cells.

Amylases↗
PubMed1988

Quantification of urinary glucose and protein with test-strips through reflectometric analysis.

Reflectometric measurement of urine test-strips, using the Urotron RL9, was employed to quantify urinary glucose and protein. Although the test-strips (Combur-9-Test) are designed for qualitative use, the reflectance intensities determined by the Urotron RL9 are applicable to quantitative analysis. Reflectance quantitation and conventional colorimetric methods were closely correlated for glucose (r = 0.953) and protein (r = 0.906). Intra-assay coefficients of variation of reflectance value were less than 5%. The negative influence of ascorbic acid on glucose determination was defined quantitatively. A significant positive interference from hemoglobin on protein determination was also demonstrated, but effectively eliminated after compensating for urine color.

Ascorbic Acid↗
PubMed1988

Serological examination of IgE- and IgG-specific antibodies to egg protein during influenza virus immunization.

The concentrations of serum IgE (PRIST) and IgE- and IgG-specific antibodies to egg protein were determined in paired sera taken from students who had received influenza virus vaccine. Although persons who gave a history of allergy to egg or to chicken feathers were excluded, 10-16% of vaccinees possessed higher titres of serum IgE and IgE-specific antibody (RAST) to egg white (F1) allergen before vaccination. The titres of IgG-specific antibody to egg protein (ovalbumin and ovomucoid antigens) were negligible, and did not show any significant response after vaccination. In contrast, IgE-specific antibody to F1 allergen rose significantly in a considerable number of the vaccines. The results obtained indicate possible contamination of vaccine products with allergens of egg origin and a potential risk of allergic manifestation after influenza vaccination.

Allergens↗
PubMed1987

Antigenic variation of Newcastle disease viruses isolated from wild ducks in Japan.

Nineteen strains of Newcastle disease virus (NDV) isolated from wild ducks in Japan were placed into 4 distinct antigenic groups on the basis of their reactivities to 8 monoclonal antibodies against the HN molecule of NDV in hemagglutination inhibition tests. The NDV strains of duck origin were antigenically distinct from NDV-B1 and NDV-Miyadera originated from chickens, and varied in their virulence to chicken embryos. No apparent correlation was found between the antigenicity of the HN molecule and virulence.

Animals↗
PubMed1986

Blood levels and distribution of talampicillin and bacampicillin in NZW rabbits.

Parameters for blood concentrations of the peroral antibiotics talampicil in and bacampicillin, in NZW rabbits were determined using a two compartment model. On the basis of these pharmacokinetic parameters, a three compartment model was prepared for the concentrations of these drugs in the tongue, gingiva, submandibular gland, parotid gland, cervical lymph-node and mandibular bone. Simulation curves based on the parameters of tissue concentrations revealed visual conformity to the measured values.

Ampicillin↗