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Biomedical subjects

N Tani

Publications and source records attributed to N Tani.

At least 109 records · Page 6Linked to original sources
PubMed1983

Method for detection of antibody against low molecular weight antigen (hapten)--production and detection of antibody against histamine H2 receptor of antibody against histamine H2 receptor antagonist "Famotidine".

Antibodies against histamine H2-receptor antagonist "Famotidine (FAMO)", molecular weight 337, chemical name; N-sulfamoyl-3-(2-guanidinothiazol-4-ylmethylthio) propionamide, were produced by subcutaneously injecting rabbits with an albumin and FAMO conjugate covalently bound with 1-ethyl-3-(3-dimethyl-amino-propyl) carbodiimide (ECDI). Two new detection systems for antibody titration were developed and employed. In one method, the antigen FAMO was tagged to sheep red blood cells (SRBC) and analysed qualitatively by a fluorescence activated cell sorter (FACS) using a second fluorescence isothiocyanate (FITC) labeled antibody. In the other method, CH-Sepharose beads were employed in place of SRBC and Horse Radish Peroxidase (HRP) was labeled to the second antibody instead of FITC used in the former method. HRP of the immune complex was colorimetrically measured with DAB-H2O2 to analyse the fine antibody titer. These high sensitive detection methods revealed the existence of IgG type of FAMO antibody. The detection sensitive detection methods revealed the existence of IgG type of FAMO antibody. The detection sensitivity was approximately 50 to 100 times higher in the later method with HRP than in the former. Furthermore these two methods could be deemed to be a good model system for a receptor assay.

Animals↗
PubMed1982

A comparative study on application of Nd-YAG laser radiation and electrocautery in canine gastric mucosa--magnified observations by dissecting microscopy and scanning electron microscopy, and histological examinations.

The mucosal change, the depth of injury and the healing process in the canine gastric wall after Nd-YAG laser (YAG) exposure and electrocautery were studied comparatively by magnified observations. The results were as follows: 1. Mucosal changes caused by the YAG laser beam were more localized within the exposure area than those by electric current. 2. The depth of tissue damage to the canine gastric wall by YAG laser exposure was easy to control when compared to that caused by electrocautery. 3. The healing time of the ulcer caused by the YAG laser was shorter than that caused by high frequency current. 4. In the present study on the mucosal changes due to laser exposure and electrocautery using endoscopy, dissecting microscopy and SEM, it was found that these examinations appear to be helpful as experimental procedures to clarify the detailed appearance of the mucosal surface. It was suggested that endoscopic application of the YAG laser to the gastrointestinal tract was safe and effective when compared with other procedures which have been applied endoscopically for diagnosis and treatment.

Animals↗
PubMed1982

Gastric acid secretion and plasma gastrin response to test meal in patients with gastric cancer.

Gastric acid secretion and plasma gastrin response to test meals were examined in 110 patients with gastric cancer and a comparative study was made in accordance with depth of invasion, macroscopic shape, histological type, location and size of the lesion. Cases were classified into two large groups by the depth of invasion: early cancer (invasion was limited to the mucosa and submucosa) and advanced cancer (invasion reached the muscularis propria or deeper). Patients showed hypoacidity on the whole. Cases of early cancer showed significantly higher acid secretion than cases of advanced cancer, although no significant differences were observed in gastrin release. Among the cases of early cancer, cases of the elevated shape showed significantly lower acid secretion and higher gastrin release than cases of the depressed shape, and cases of the histologically differentiated type showed significantly higher acid secretion than cases of the undifferentiated type although no significant differences were observed in gastrin release. Among the cases of advanced cancer, cases with large carcinomas in the corpus tended to show low acid secretion and cases with large carcinomas in the antrum tended to show low gastrin release. High gastrin release was observed in cases of the Borrmann IV type with giant folds.

Aged↗
PubMed1982

Relation between esophagogastric junction and esophageal function in patients with reflux esophagitis.

The relationship between the esophagogastric junction (Z-line pattern) and the functions of the esophagus was studied in patients with reflux esophagitis. We classified Z-line patterns as the Z0- type to Z4-type, depending on the shape and the level of the Z-line. Lower esophageal sphincter pressure (LESP) was measured by the open-tip infusion method and the acid clearance test was performed by Booth's method (15 ml of 0.1 HCL). The following results were obtained: 1. LESP was 15.5 +/- 5.6 cmH2O (M +/- SD) in normal subjects (Z0-type), it was 10.3 +/- 2.9 cmH2O in the Z1-type, 9.5 +/- 4.9 cmH2O in the Z2-type, 9.4 +/- 4.1 cmH2O in the Z3-type and 7.6 +/- 3.4 cmH2O in the Z4-type. There were statistically significant differences between the Z0-type and the other Z-line patterns (p less than 0.05, P less than 0.05, P less than 0.025, and P less than 0.025, respectively). 2. Acid clearance was expressed as the time until the pH returned to 5.0 due to swallowing at one-minute intervals. The results of the acid clearance test were 11.4 +/- 3.0 min in the Z0-type 18.3 +/- 7.0 min in The Z1-type, 20.3 +/- 5.6 min in the Z2-type, 24.1 +/- 5.7 min in the Z3-type and 24.5 +/- 7.4 min in the Z4-type. Acid clearances were significantly delayed along with the Z-line pattern (P less than 0.025, P less than 0.005 and P less than 0.005, respectively). 3. The patients with hiatal hernia consisted of five cases of the Z3-type and six cases of Z4-type. They had low LESP and delayed acid clearance. As mentioned above, the results of LESP ad acid clearance test were well correlated with Z-line patterns in patients with reflux esophagitis.

Aged↗
PubMed1982

[Secretin].

Explore the source record for details and available documents.

Duodenal Ulcer↗
PubMed1982

Epidemio-hygieological aspects of gastric neoplasms in aged patients.

A 100-year-old male who died of gastric carcinoma was discussed and illustrated epidemiohygieologically. According to dynamic population statistics of the Health and Welfare Ministry of Japan, 418 persons over the age of 100 years died in 1979. Among them, 157 (38%) died of cardiovascular diseases and 49 (12%) died of pulmonary diseases, but only six died of neoplasms including two with gastric carcinomas (0.48%). To our knowledge no such case has been reported previously in the literature.

Adenocarcinoma↗
PubMed1981

Retinotopic analysis of fiber pathways is amphibians. I. The adult newt Cynops pyrrhogaster.

The possibility that pathways of retinal fibers within the optic tract and the tectum of the adult newt are retinotopic was examined by selective labeling of the retinal fibers with horseradish peroxidase. Within the optic tract fibers from the ventral, temporal and dorsal retinal quadrants were ordered from the dorsal to ventral edges of th optic tract. The nasal retinal fibers exhibited two different pathways. The fibers from the dorsonasal retina ran along the ventral edge of the optic tract, while the fibers from the ventronasal retina ran along the dorsal edge of the optic tract. Segregation of pathways within the optic tract was incomplete between the nasal and other retinal fibers. The dorsonasal retinal fibers were mixed completely with the dorsal retinal fibers, and the ventronasal retinal fibers were mixed partly with the ventral retinal fibers. Both the dorsal and dorsonasal retinal fibers preferentially entered the lateral tract, and finally projected onto the ventrolateral parts of the middle tectum and of the caudal tectum, respectively. The ventral and ventronasal retinal fibers entered the dorsomedial tract, and projected onto the dorsomedial parts of the middle tectum and of the caudal tectum, respectively. The temporal retinal fibers invaded the nasal tectum directly. Most dorsal, ventral, and nasal retinal fibers ran along the sub-tracts as far as to the level of their terminals, then sharply turned in a direction to the tectum.

Afferent Pathways↗
PubMed1981

Retinotopic analysis of fiber pathways in amphibians. II. The frog Rana nigromaculata.

The possibility of retinotopic organization of pathways of retinal fibers within the optic tract and the tectum of the frog was studied by selective labeling of the retinal fibers with horseradish peroxidase. Within the optic tract the pathways of the ventral, temporal and dorsal retinal fibers were ordered from the dorsal to ventral edges of the optic tract. The nasal retinal fibers ran along both the dorsal and ventral edges of the optic tract. The dorsal retinal fibers and the nasal retinal fibers which were located along the ventral edge of the optic tract entered the ventrolateral perimeter of the tectum and formed the lateral tract. The ventral retinal fibers and the nasal retinal fibers which were located along the dorsal edge of the optic tract entered the dorsomedial perimeter of the tectum and formed the dorsomedial tract. The temporal retinal fibers invaded the tectum directly at the diencephalo-tectal junction. The topography of fiber pathway observed for the frog was exactly the same as that seen in the newt, and seemed to be common to all amphibian species.

Afferent Pathways↗
PubMed1981

Correlative ontogenetic development of catecholamine- and LHRH-containing nerve endings in the median eminence of the rat.

The ontogenetic development of catecholamine (CA)- and LHRH-containing nerve endings in the median eminence of the rat was investigated by combining fluorescence histochemistry and immunohistochemistry in the same tissue section. LHRH-terminals appeared earlier than CA-terminals and were already detectable in the lateral part of the external layer of the central ME on the first day after birth. CA-nerve endings were first seen in a corresponding region of the ME on the seventh postnatal day. At this stage both types of terminals showed the earliest manifestation of a correlative pattern of their distribution. Subsequently the development of both types of nerve endings proceeded rapidly, and at 14 days their distribution pattern corresponded to that in adult animals. The authors conclude that at this stage the CA-neurons play a constant and significant role in the release of LHRH into the portal capillaries. The correlation between both types of nerve endings and the ontogenetic development of the capillary plexuses of the hypophysial portal system is discussed.

Age Factors↗
PubMed1981

The coexistence of gastric cancer and duodenal ulcers.

The records of 356 gastric carcinoma patients who were treated surgically were reviewed. Among them, six patients (1.7%) had duodenal ulcers. Five cancers of the six patients were "early" gastric cancers classified as type "IIc" or "IIc + III" according to "The general rules for the gastric cancer study in surgery and pathology". The cancers of the six patients were located in the lower half of the stomach. Gastric secretion activity was normal in four and above normal in two cases. Three had signet ring cell carcinomas, two had poorly differentiated adenocarcinomas and one had a well differentiated adinocarcinoma. Our results were similar to other results reported in many papers. In Japan gastric carcinomas coexisting with duodenal ulcers were more often confirmed in the "early" gastric cancer stage. This is probably because endoscopic examinations of the stomach were more often performed in Japan than in other countries because of te large number of gastric disease patients. In addition, it is assumed that the growth of gastric cancer in the stomach is slow because of the very active gastric secretion.

Adenocarcinoma↗
PubMed1981

The effect of test meals on the PED test.

The effects of test meals on the PFD (pancreatic function diagnostant) test, which was introduced as "the B.T.PABA test" in the previous reports, were studied. The higher the protein in the test meal, the lower the urinary excretion value of p-aminobenzoic acid (PABA) and the first 3-h of PABA excretion. By using a test meal containing protein, the differences in digestion-absorption disorders between gastrectomy procedures (Billroth I and Billroth II) were clarified. Therefore, a test meal which contains a suitable amount of protein should be used in the PFD test to improve diagnostic accuracy.

4-Aminobenzoic Acid↗
PubMed1981

Hematemesis and Melena: gastritis.

The records of a total of 60 gastritis patients who showed hematemesis and/or melena are reviewed. The mean age was 40. The ratio of males to females was two to one. During the examination, 19 upper G-I lesions were confirmed in addition to gastritis. Sixteen patients had severe underlying diseases which were thought to be the cause of the bleeding tendency. Thirteen patients took alcohol or drugs which induced bleeding. Prognoses of the patients were relatively good when they were treated medically.

Adolescent↗
PubMed1981

The PFD test for pancreatic disease patients.

The PFD test was performed on various pancreatic disease patients. The urinary PABA excretion rate was significantly lower in chronic pancreatitis patients and in pancreatic carcinoma patients than in the controls, but no difference was observed between PABA excretion in the two diseases. After surgery, PABA excretion values of pancreatic carcinoma patients were significantly lower than those of non-pancreatitis non-pancreatic carcinoma patients. This was thought to be caused by the difference in residual anastomosed pancreatic tissues. Mild to severe pancreatitis, fibrosis and/or fat necrosis were seen in the pancreatic tissues of carcinoma patients, but these changes were minimal in non-pancreatitis non-pancreatic carcinoma patients. The PFD test is useful in detecting decreases in pancreatic exocrine functions or measuring the grade of pancreatic disorders in various pancreatic diseases, although it can not be used to differentiate pancreatic diseases.

4-Aminobenzoic Acid↗