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Biomedical subjects

N R Mehta

Publications and source records attributed to N R Mehta.

65 records · Page 4Linked to original sources
PubMedDate not supplied

Comparison of the cholesteryl ester composition of human milk from preterm and term mothers.

Milk was obtained on postpartum days 2-3 (colostrum) and days 7, 21, 42, and 84 from mothers of 18 very premature (VPT, 26-30 weeks gestational age), 28 premature (PT, 31-36 weeks), and 6 term (T, 37-40 weeks) infants. Lipids were extracted in chloroform-methanol and analyzed by thin-layer (TLC) and gas liquid chromatography (GLC). Fatty acid composition of cholesteryl esters was determined by GLC after isolation of cholesteryl esters by preparative TLC and preparation of methyl esters of the constituent fatty acids. As lactation progressed, amounts of total cholesterol and cholesteryl esters declined. Cholesteryl esters decreased from about 5 mg/dl in colostrum to 1 mg/dl in mature milk. The cholesteryl esters of colostrum from mothers of premature infants were different in fatty acid composition from those of term infants. Proportions of medium-chain saturated fatty acids (12:0, 14:0, 16:0) of preterm colostrum (VPT and PT) were considerably lower than in term colostrum: 23% of total fatty acids versus 35%. Proportions of 18:3, 20:3, and 20:4 of VPT (5.6%) and PT (6.2%) colostrum were considerably higher than T colostrum (1.8%). The fatty acid composition of cholesteryl esters of VPT, PT, and T milk was relatively similar at all subsequent lactation periods. Fatty acids esterified with cholesterol in weight percents were as follows: 10:0, 0.7; 12:0, 2.6; 14:0, 2.3; 16:0, 11.4; 16:1, 5.0; 18:0, 8.8; 18:1, 32.9; 18:2, 30.6; 18:3, 1.7; 20:3, 0.9; and 20:4, 1.8. Unsaturated fatty acids contributed 73 wt % of fatty acids in cholesteryl esters, which is considerably higher than in milk triglycerides. The greatest difference occurred in 18:2 content, which was 30.6% in cholesteryl esters and only 13.0% of total fatty acids in milk. Results suggest that unsaturated fatty acids are associated preferentially with the cholesteryl ester fraction and that the fatty acid composition of cholesteryl esters differs from the composition of total milk lipid.

Cholesterol↗
PubMedDate not supplied

Bile salt-stimulated lipase of human milk: characteristics of the enzyme in the milk of mothers of premature and full-term infants.

Human milk contains a lipase (bile salt-stimulated lipase) that is considered to have an important role in infant fat digestion. In this study we compared the characteristics of bile salt-stimulated lipase activity in milk samples from mothers delivering prematurely (26-30 and 31-37 weeks of gestation) and in milk from mothers delivering at term (38-42 weeks of gestation). Preterm milks were collected at day 1-5 and during week 6 of lactation. Term milks were collected during week 6 of lactation. The characteristics of the enzyme (kinetics, enzyme concentration, pH optimum, and pH stability, effects of bile salt structure and concentration, eserine inhibition) were identical regardless of length of pregnancy or duration of lactation. Bile salt-stimulated lipase had a neutral to alkaline pH optimum (pH 7.3-8.6), was stable for 1 h at a wide pH range (pH 3.1-8.6), was active only in the presence of primary bile salts, and was inhibited by eserine. The data indicate that, following parturition at as early as 26 weeks of gestation, the mammary glands synthesizes bile salt-stimulated lipase with identical characteristics as does the mammary gland after a full-term pregnancy.

Bile Acids and Salts↗
PubMedDate not supplied

Hyperalimentation-associated neonatal cholestasis: effect of oral gentamicin.

The effect of oral gentamicin on the incidence of parenteral nutrition-associated cholestasis in preterm infants less than 1500 g birth weight was assessed retrospectively. Of 24 patients on parenteral nutrition for more than 10 days, 12 infants who received oral gentamicin (group I) for prophylaxis against neonatal necrotizing enterocolitis were compared to 12 infants who did not (group II). Both mean and peak direct bilirubin levels were significantly higher in group II. The increase in both mean and peak direct bilirubin levels after initiation of total parenteral nutrition (TPN) was significant in group II only. The incidence of cholestasis was significantly higher in group II than in group I. These results suggest that oral gentamicin may have a protective effect against parenteral nutrition-associated cholestasis in the newborn preterm infant.

Cholestasis↗
PubMedDate not supplied

Pulmonary function and morbidity in textile mill workers.

The study was conducted in a large cotton textile mill in Surat City. A selected number of 278 employees were evaluated for social, anthropological and pulmonary functions. No parameters except the period of exposure to dust reduced pulmonary function; it was statistically significant in the case of vital capacity and maximum breathing capacity. Out of 23.94% pulmonary morbidity observed, the byssinosis rate was 1.62.

Byssinosis↗