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Biomedical subjects

N Ogino

Publications and source records attributed to N Ogino.

At least 91 records · Page 5Linked to original sources
PubMed1986

Growth factors induce actin disruption in cultured human retinal pigment epithelial cells.

Exposure of cultured human retinal pigment epithelial cells to platelet-derived growth factor, nerve growth factor or epidermal growth factor resulted in a time- and dose-dependent alteration in the distribution of actin stained by rhodamine-phalloidin. These growth factors (platelet-derived growth factor of 80 ng/ml, nerve growth factor of 10 ng/ml or epidermal growth factor of 10 ng/ml) caused disappearance of actin filaments from the peripheral region of a cell in 1 or 2 h and change of cell configuration to spindle shape in 3 or 4 h. Other growth factors, fibroblast growth factor of 10 ng/ml and insulin of 25 mumol/ml had no effect on actin distribution. The alteration of actin and the change of cellular shape might be associated with stimulation of cell growth and migration of retinal pigment epithelial cells.

Actins↗
PubMed1986

Chick retinal pigment epithelium exhibits glutathione requiring prostaglandin D2 synthetase activity.

Prostaglandin synthetic activity of neural retina and retinal pigment epithelium in chick retina was studied using arachidonic acid and prostaglandin H2 (intermediate prostaglandin endoperoxide) as substrates. To obtain sufficient enzyme activity in retinal pigment epithelium, cultured chick retinal pigment epithelial cells were used. Little arachidonic acid was converted into prostaglandins, and details of prostaglandin synthetic activity were not clear. The assay from prostaglandin H2 showed that: 1) preparations consisting of both neural retina and retinal pigment epithelium dissected from 7-day-old chicks have activity of glutathione-requiring prostaglandin D2 synthetase; 2) preparations consisting of only neural retina dissected from 7-day-old chicks have no activity of prostaglandin synthetase; and 3) cultured chick retinal pigment epithelial cells have high activity of glutathione-requiring prostaglandin D2 synthetase.

Animals↗
PubMed1985

Exocytosis by retinal pigment epithelial cells.

We observed cultured retinal pigment epithelial cells as they responded to the introduction of latex particles. The cells showed phagocytosis of particles 1 h after administration of latex and were filled with particles after 24 h. After 7 days, exocytosis of latex from basal plasma membrane was documented. Observation was repeated using a two-layer culture. 7 days after putting the retinal pigment epithelial layer containing latex particles on another layer without particles, we observed the appearance of latex particles in the lower layer that originally contained no particles. This demonstrated that cultured retinal pigment epithelial cells exocytose latex particles from basal cell membrane.

Animals↗