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Biomedical subjects

N Nakano

Publications and source records attributed to N Nakano.

At least 91 records · Page 5Linked to original sources
PubMed1989

Bacterial overgrowth in the jejunum of ICR mice and Wistar rats orally administered with a single lethal dose of fusarenon-X, a trichothecene mycotoxin.

A single oral dose of fusarenon-X (F-X), a trichothecene mycotoxin, resulted in abnormal microflora in the jejunum in ICR mice and Wistar rats with some differences in dose response between the species. In the acute phase, enterobacteria, streptococci, Clostridium perfringens and bacteroides showed remarkably increased counts in the jejunum of mice and rats dosed with F-X while lactobacilli showed a decrease in count. F-X brought an invasion of Pseudomonas aeruginosa into the livers, lungs, kidneys and spleens of ICR mice. Changes in the jejunal microflora appeared after 7 h in ICR mice and after 24 h in Wistar rats after a single oral dose of F-X of 7.5 and 4.0 mg/kg b.w., respectively, and the microflora returned to its normal state at 72 h in mice and 96 h in rats. The changes of intestinal microflora were followed by alterations in the growth curves of both animal species. The pH in the glandular stomach was also greatly enhanced before changes in the jejunal microflora. Acute F-X intoxication may be an involved manifestation of essential cytotoxicity of F-X mycotoxin alone and secondary bacterial overgrowth in the bowel.

Animals↗
PubMed1989

[IgE-Fc receptor expressions on bronchoalveolar lavage (BAL) cells in a patient with eosinophilic pneumonia accompanied with bronchial asthma--the effect of ketotifen on their expression].

In this study, IgE-Fc receptor expressions on each type of BAL cells (lymphocytes, macrophages and eosinophils) in a patients with eosinophilic pneumonia accompanied by bronchial asthma were examined by indirect immunofluorescent method using monoclonal antibody (H107). BAL cell findings showed marked increases of total BAL cell counts and eosinophils, an increased number of lymphocytes and the presence of basophilic cells. These results match those of our previous report. Furthermore, IgE-Fc receptor expressions on lymphocytes, macrophages and eosinophils were markedly increased as compared to those in the peripheral blood and normal control subjects. These findings suggest that IgE production in the lung plays the main role in the pathogenesis of eosinophilic pneumonia and bronchial asthma. On the other hand, during ketotifen administration, decreases of peripheral blood eosinophilia, of eosinophilia in the sputum and of serum IgE level and an improvement in chest X-ray findings were observed. Furthermore not only normalization of total BAL cell counts but also decreases of IgE-Fc receptor expressions on BAL cells were observed.

Adult↗
PubMed1989

[The inhibitory effect of oxatomide, an anti-allergic agent, on eosinophil-mediated and eosinophilic cell line-mediated natural cytotoxicity against bronchial epithelial cells].

Recently, eosinophils have been implicated as inflammatory effector cells in allergic and inflammatory reactions such as bronchial asthma. In this study eosinophil-mediated and eosinophilic cell line-mediated natural cytotoxicity against bronchial epithelial cells and the effects of oxatomide, an anti-allergic agent, on their cytotoxicity were investigated. Treatment with oxatomide diminished both eosinophil-mediated and eosinophilic cell line-mediated natural cytotoxicity in vitro. We concluded from these results that oxatomide not only has anti-allergic activity but also anti-inflammatory properties for eosinophils. In addition this method for isolating eosinophils seems to well serve the purpose of evaluating eosinophil function as in this investigation, as we have reported previously.

Bronchi↗
PubMed1989

[The discussion of the need of mediastinal lymph node dissection in the surgical treatment of lung cancer].

The need of lymph node dissection in the case of operative lung cancer with negative mediastinoscopy was discussed. As a preliminary study, the N-number obtained from the mediastinoscopy and that of postoperative examination was compared. The rate of agreement of N-number was as high as 96%. Secondary, 183 cases of lung cancer with squamous-or adenocarcinoma pathologically T-1 or 2. N-0 and P-0 or 1 were picked up and examined. In those cases, the result of 3 years survival rate was 80% in the cases of absolute curative operation and 60% in that of relative non-curative cases, and 5 years survival rate was 70% and 52% respectively, and the cases of absolute curative operation was statistically excellent than that of another group. However, the background of the two groups was different to some degree on account of the advancement of the cancer and the age of the patients. Therefore, the randomized study as for the lymph node dissection in the cases of negative mediastinoscopy was finally performed. In the 30 cases of squamous cell carcinoma where dissection was carried out in 13 cases and not done in 17 cases, the result of 3 years survival was 84% (dissecting group) and 89% (non-dissecting group), and 5 years survival was 73% and 63% respectively. In 23 cases of adenocarcinoma (13; dissecting, 10; non-dissecting), the result of 3 years survival was 100%, 63% and that of 5 years survival rate was 100%, 63% respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

Adenocarcinoma↗
PubMed1988

Comparison of the results of laminectomy and open-door laminoplasty for cervical spondylotic myeloradiculopathy and ossification of the posterior longitudinal ligament.

Laminectomy was performed on 14 patients with cervical radiculomyelopathy and ossification of the posterior longitudinal ligament (OPLL). Since 1978, simple open-door laminoplasties have been performed on 75 patients. A retrospective study was done to determine if there was any difference between the results of laminectomy and laminoplasty. Overall results of laminectomy were as follows: The preoperative Japanese Orthopaedic Association (JOA) score was 7.0 points and the postoperative score was 15.0 points. Overall results of laminoplasty were as follows: the preoperative JOA score was 7.0 points and the postoperative score was 15.0 points. The percentage improvement in laminectomy was 81.1%, and in laminoplasty was 81.4%. There was no significant difference in the results of these two procedures for the treatment of patients with cervical radiculomyelopathy and OPLL.

Adult↗
PubMed1988

Clinical results following enlargement of the cervical spinal canal by means of laminoplasty.

Since 1978, cervical spinal canal laminoplasty has been performed on 75 patients in our hospital with cervical radiculomyelopathy and followed with studies of from 6 months to 8 years (average 4 years and 6 months). Overall results: the pre-operative score, using the Japanese Orthopaedic Association Scoring System, was 7.4 and the post-operative score was 15.2; the improvement was 81.0%. In congenital spinal stenosis, the average pre-operative score was 6.3 while the post-operative score was 14.2; the improvement was 66.9%. The pre-operative score of multiple disc lesions was 7.9 and the post-operative score was 15.2; the improvement was 82.8%. And the pre-operative score of OPLL was 7.3 and the post-operative score was 15.3; the improvement was 81.9%. Improvement may be due not only to the degree of enlargement of the spinal canal, but also to improvement in the circulation within the spinal cord and nerve roots. No case worsened after the surgery.

Female↗
PubMed1987

Identification of intrathymic T progenitor cells by expression of Thy-1, IL2 receptor and CD3.

Immature (L3T4-/Lyt-2- "double-negative") thymocytes were separated into several functionally distinct fractions based on their expression of IL2 receptors, Thy-1 and CD3. The majority (60-70%) of double-negative thymocytes in young adult mice lack detectable IL2 receptor expression, have high levels of Thy-1 and rapidly "progress" to a L3T4+ or L3T4+/Lyt-2+ stage when cultured for 20 h in simple medium. In contrast, the IL2 receptor-positive fraction retains the double-negative phenotype for as long as it survives in culture and addition of IL2 has little or no effect on such cells. IL2 does generate strong proliferation from a fraction of cells expressing low levels of Thy-1, but not detectable IL2 receptors. Such culture generates an unusual population of double-negative cells that expresses the pan-B cell molecule B220 and which kill both the NK target cell line YAC-1 and the NK-resistant line EL4. This Thy-1-low fraction includes all of the double-negative thymocytes capable of T cell reconstitution. Thy-1-low fraction could be further separated into two populations with regards to CD3 expression. CD3- but not CD3+ population could reconstitute mature T cells, indicating that Thy-1-low, IL2R- and CD3- cells are the most enriched population of intrathymic T cell progenitors.

Animals↗
PubMed1987

Human B-cell differentiation factor defined by an anti-peptide antibody and its possible role in autoantibody production.

The partial amino acid sequence of the NH2 terminus of a factor named human B-cell differentiation factor or B-cell stimulatory factor 2 (BSF-2) has been determined. Antibodies raised against the synthetic peptide corresponding to residues 1-13 of the NH2-terminal sequence specifically react with BSF-2 generated by a T-cell line and by phytohemagglutinin-stimulated normal T cells. Furthermore, the antipeptide antibodies react with a BSF-2-like factor produced by cardiac myxoma as well as uterine cervical carcinoma cells. The results show that BSF-2 functions in vivo as well and suggest that the constitutive production of BSF-2 may be involved in autoantibody production, since patients with cardiac myxoma and uterine carcinoma showed autoantibody production.

Amino Acid Sequence↗
PubMed1987

[Clinical long-term studies on cefuzoname in patients with complicated urinary tract infections].

Cefuzoname (CZON), a new semisynthetic cephalospolin antibiotic, was administered intravenously at a dose of 1.0 g twice a day for 14 days to 20 patients with complicated urinary tract infections and the therapeutic efficacy of the drug and safety were evaluated. The results obtained were summarized as follows: 1. After 5 days, clinical results were excellent in 7, moderate in 3 and poor in 9 cases. The overall effectiveness rate was 52.6%. Bacteriologically, 24 out of 32 isolated strains were susceptible to this drug, but 8 strains were found to be resistant. 2. After treatment, clinical results were excellent in 6, moderate in 3 and poor in 10 cases. The overall effectiveness was 47.4%. In bacteriological results of 32 isolated strains, 26 strains were eradicated and 6 strains persisted. 3. Among 20 cases treated none showed subjective or objective symptoms of side effects. Noted as clinical laboratory test abnormalities were eosinophilia in 1 case after 5 days, increase of S-GOT, gamma-GTP, and K in 1 case, and increase of S-creatinine in 1 case at the termination of treatment. All of these abnormalities were mild and normal values were restored soon after at the termination of the treatment.

Adult↗
PubMed1985

Purification to homogeneity and characterization of human B-cell differentiation factor (BCDF or BSFp-2).

Human B-cell differentiation factor (BCDF) was purified to homogeneity by sequential filtration and chromatography of culture supernatants from TCL-Na1 cells on an AcA34 gel column and then on a Mono P column with fast protein liquid chromatography and reversed-phase HPLC. A 5300-fold enrichment in specific activity of BCDF with about 25% recovery was attained. The homogeneity of purified BCDF was evidenced by the following: (i) the specific activity was 1.7 X 10(7) units/mg of protein, (ii) only two bands, Mr 19,000 and 21,000, were identified by NaDodSO4/PAGE under reduced as well as nonreduced conditions, and (iii) BCDF activity was recovered from the gel after NaDodSO4/PAGE in the fractions corresponding to protein bands of Mr 19,000 or 21,000. Purified BCDF induced Ig secretion in Epstein-Barr virus-transformed cell lines; as little as 3 pM gave 50% of the maximum reaction achieved by 30-80 pM BCDF. Purified BCDF induced Ig production in activated B cells without any effect on cell growth. Purified BCDF did not show any activity of interleukin 1 or 2, B-cell stimulatory factor (BSF)p-1, B-cell growth factor II (BCGF-II), or interferon. Since BCDF was isolated and characterized as described, we propose that the BCDF that induces the final differentiation of B cells into high-rate Ig-secreting cells be designated BSFp-2.

Antigens, Differentiation, B-Lymphocyte↗
PubMed1985

Immortalization of BGDF (BCGF II)- and BCDF-producing T cells by human T cell leukemia virus (HTLV) and characterization of human BGDF (BCGF II).

Human peripheral T cells were transformed by human T cell leukemia virus (HTLV), and T cell lines producing BGDF (BCGF II) and BCDF were established. Among these cell lines, a cell line, TCL-Na1, secreted the highest level of both BGDF and BCDF, and the amount of BCDF secreted by TCL-Na1 cells was 900-fold more than that produced by PHA-stimulated T cells. Within the limits of our examination, none of the HTLV-transformed T cell lines produced IL 2 or BSF-p1 (BCGF I). BCDF produced by TCL-Na1 cells had a m.w. of 35,000 and a pI value of 5.5, being separated from BGDF, which was eluted in the fractions corresponding to m.w. of more than 60,000 and pI values of 5 to 6. BGDF induced both proliferation and IgM secretion in a mouse leukemic B cell line, BCL1, and these activities were not separated by either isoelectric focusing or gel filtration in the presence or absence of 0.1% Triton X-100, suggesting that the molecule designated BGDF exerted both growth and differentiation activities. BGDF acted on normal mouse B cells to induce proliferation as well as IgM secretion. The target cells of BGDF were in vivo activated B blast cells. BGDF acted on DXS-activated murine B cells to induce both proliferation and IgM secretion but not anti-Ig-activated B cells, indicating that BGDF and BSF-p1 were different molecules.

Animals↗
PubMed1985

[Gastric cancer and serum zinc content].

Changes in the serum zinc content of patients with gastric cancer were investigated in connection with the size of the primary lesion, the depth of invasion into the gastric wall, the presence or absence of liver metastasis and the histological types. The serum zinc content of the patients decreased along with the progress of the cancer. The changes in serum zinc were closely related to the extent and severity of the disease, but were independent of the presence of liver metastasis and of the histological types in patients with gastric cancer. As a cause of decreasing serum zinc in cancer patients, a decrease of serum albumin was emphasized. The relationship between the decrease of serum zinc and accumulation of zinc in the tumor tissue in patients with gastric cancer could not be elucidated.

Animals↗
PubMed1984

White goddess--the unspeakable name. An inquiry into Robert Graves' King Jesus.

Robert Graves is a poet-mythographer, well-known in the U. K. and the U. S. A., but not in Japan despite his huge amount of poetic, mythographical, prose and critical works. Furthermore, even in the U. S. A. and the U. K., some people have been shunning him politely as a versatile iconoclast possessed of the White Goddess. In fact, it is not difficult to imagine that when King Jesus, a life story of Christ, was published, people were shocked at his extraordinary mythographical Christology derived from his enthusiasm towards the White Goddess Cult. In Christ he discovered the fragments of maternal doctrine as a new concept in opposition to the Apollonian theory of Judaism, but they seemed to be quite incomplete in the author's eyes lacking in something most essential in life, recognition of love and hatred discipline. This paper is an inquiry into how the author developed his own hermaneutics of Christ through his wide and thorough scholarship on mythology, history, the Bible and Celtic poetry.

Christianity↗