Biomedical subjects

N Nakabayashi

Publications and source records attributed to N Nakabayashi.

At least 37 records · Page 2Linked to original sources

PubMed2000

Small diameter vascular prosthesis with a nonthrombogenic phospholipid polymer surface: preliminary study of a new concept for functioning in the absence of pseudo- or neointima formation.

The purpose of this study was to prepare a small diameter vascular prosthesis functioning without pseudointima formation. A nonthrombogenic phospholipid polymer, the 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer, has a cell membrane-like structure and has demonstrated strong nonthrombogenicity. We have recently prepared 2 kinds of vascular prostheses, 2 mm in diameter, composed of the MPC polymer and segmented polyurethane (SPU). One includes 7.5 wt% MPC polymer (SPU/MPC[7.5] prosthesis), and the other includes 10.0 wt% (SPU/MPC[10] prosthesis). These prostheses were placed in rabbit carotid arteries and were retrieved at 1 and 4 weeks after implantation. A pseudointima was observed at 4 weeks on the SPU/MPC(7.5). For the SPU/MPC(10), the surface was macroscopically clear without a pseudointima even after a 4 week implantation. It appears that the SPU/MPC(10) prosthesis, functioning without a pseudointima, possesses a stronger nonthrombogenicity and would be more applicable for clinical use.

Anastomosis, Surgical↗

PubMed1999

Stabilization of an antibody conjugated with enzyme by 2-methacryloyloxyethyl phosphorylcholine copolymer in enzyme-linked immunosorbent assay.

The purpose of this study was to develop a novel synthetic stabilizer of enzyme-linked antibody in the enzyme-linked immunosorbent assay (ELISA). The water-soluble amphiphilic phospholipid polymer, poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-co-styrene (St)] was synthesized, and its stabilizing functions for the antibody were compared with conventional stabilizers of the antibody conjugated with enzyme (enzyme-antibody conjugate), such as bovine serum albumin (BSA) and casein. In the absence of the stabilizer, the remaining immunologic activity decreased to about 10% of its initial value after 37 days. The same tendency was observed even when the enzyme-antibody conjugate in 1.0 wt % BSA solution was used as a stabilizer. In 1.0 wt % casein solution, the immunologic activity decreased to 29% of the initial value after 37 days. On the other hand, in 0.1 wt % and 1.0 wt % poly(MPC-co-St) solution, the activity remained 74% and 92% of the initial value, respectively. The effects of poly(MPC-co-St) on the stabilization of the enzyme-antibody conjugate depended on the concentration of poly(MPC-co-St). During the ELISA procedure, not only did poly(MPC-co-St) have no effect on the reaction between the antigen and the antibody, but it also had no effect on the reaction between the enzyme and the substrate. These results indicate that poly(MPC-co-St) has the ability to suppress the denaturation of protein, enzyme, and antibody. We concluded that water-soluble poly(MPC-co-St) is an effective synthetic stabilizer in the ELISA.

Animals↗

PubMed1999

Behavior of blood cells in contact with water-soluble phospholipid polymer.

Omega-Methacryloyloxyalkyl phosphorylcholine (MAPC) polymer, which has various methylene chain lengths between the phosphorylcholine group and the backbone, was synthesized with attention to formation of the biomembrane. The effect of water-soluble poly(MAPC) on the function and activation of blood cells was evaluated to determine the interaction between blood cells and the MAPC polymer. The poly(MAPC) and the MAPC copolymer with a small amount of fluorescent units were synthesized by a conventional radical polymerization technique. Using a fluorescence spectrometer, it was determined that the MAPC polymer was adsorbed on the plasma membrane of platelets when the platelets were suspended in an aqueous solution of the MAPC copolymer. The hemolytic activity of poly(MAPC) was less than that of other water-soluble polymers, such as poly(ethylene glycol) and poly(1-vinyl-2-pyrrolidone) (PVPy). The change in the plasma membrane fluidity of platelets on contact with poly(MAPC) was determined with 1,6-diphenyl-1,3,5,-hexatriene. The plasma membrane fluidity of platelets decreased with an increase in the methylene chain length of the MAPC unit. The aggregation activity of platelets after contact with poly(MAPC) was also evaluated, but no significant difference between that of polymer-contacted platelets and native platelets was observed. Finally, the activity of platelets on contact with poly(MAPC) was determined by measuring the cytoplasmic calcium ion concentration ([Ca2+]i) in platelets. The increase in [Ca2+]i in the platelets after contact with poly(MAPC) was similar to that of native platelets. We conclude that the poly(MAPC) reduced platelet activation even though the poly(MAPC) adsorbed on the membrane surface of the platelets. In particular, poly(10-methacryloyloxydecyl phosphorylcholine) significantly reduced platelet activation compared with PVPy.

Adsorption↗

PubMed1999

Total hip arthroplasty using bone cement containing tri-n-butylborane as the initiator.

We performed total hip arthroplasty (THA) using a special acrylic self-curing bone cement (Bonemite), which contains tri-n-butylborane as the initiator. Its maximum temperature at curing is lower than that of a conventional bone cement (CMW). Fifty-eight THAs using Bonemite and 35 THAs using CMW were followed up for more than 8 years (12.5 years on average). At the 10-year follow-up, the survival rates, using revision surgery or aseptic loosening impending revision as the endpoint for failure, were 92.2% for the patients in the Bonemite group and 91.0% for those in the CMW group. No statistical differences were observed between the patients in these two groups with regard to survival rate (p = 0.39). Bonemite showed no clear superiority compared with CMW, although the results suggest that Bonemite is safe and reliable for clinical use and stable in situ for long time.

Arthroplasty, Replacement, Hip↗

PubMed1999

The role of recombinant human bone morphogenetic protein-2 in PLGA capsules at an extraskeletal site of the rat.

Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGF-beta) superfamily and has strong bone-inductive activity in vivo. To examine the role of BMP-2 in an extraskeletal site of rat using a controlled release system of peptides, we encapsulated the recombinant human BMP-2 (rhBMP-2) with poly(DL-lactide-co-glycolide) (PLGA) and implanted the rhBMP-2/PLGA capsules in the subcutaneous area of rats. Upon histochemical examination, it was found that bone-inducing cells having alkaline phosphatase (ALP) activity appeared around the capsules by the suitably released rhBMP-2. In addition, the temporal histological examination showed that direct bone formation without cartilage occurred in the process of this ectopic bone induction. These data indicate that the role of rhBMP-2 in the extraskeletal site of rats is to induce the differentiation of mesenchymal cells into the osteoblasts.

Alkaline Phosphatase↗

PubMed1999

Modification of polysulfone with phospholipid polymer for improvement of the blood compatibility. Part 2. Protein adsorption and platelet adhesion.

Protein adsorption and platelet adhesion from human plasma on polysulfone (PSf) membranes modified with 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer were studied. The modification was carried out by blending of the MPC polymer in the PSf. The amount of protein adsorbed on the PSf/MPC polymer blend membrane was significantly decreased with an increase in the composition of the blended MPC polymer. The distribution of the specific proteins adsorbed on the membrane surface was also determined by a gold-colloid immunoassay. Albumin, gamma-globulin and fibrinogen were observed on every membrane surface after contact with plasma. However, in the case of the blended membrane, the density of the adsorbed proteins decreased compared with that of original PSf membrane. That is, the MPC polymer blended in the membrane could function as a protein-adsorption-resistant additive. The number of platelets adhered on the PSf membrane was reduced, and change in the morphology of adherent platelets was also suppressed by the modification with the MPC polymer. Therefore, the PSf/MPC polymer blend membrane had improved blood compatibility compared with the PSf membrane.

Adsorption↗

PubMed1999

Modification of polysulfone with phospholipid polymer for improvement of the blood compatibility. Part 1. Surface characterization.

To improve the surface blood compatibility of polysulfone (PSf) membranes, we prepared novel polymeric additives which have suitable blood compatibility. They were polymers with a phosphorylcholine group, a 2-methacryloyloxyethyl phosphorylcholine (MPC) unit. The MPC polymer could be blended with polysulfone by a solvent evaporation method during membrane processing, and a transparent membrane could be obtained. The mechanical properties of the blend membrane were similar to that of the original PSf membrane. Surface analysis of the blend membrane by X-ray photoelectron spectroscopy and dynamic contact angle measurement revealed that the MPC unit in the polymeric additive was concentrated on the surface of the membrane. The blend membrane significantly reduced plasma protein adsorption compared with that of the PSf membrane.

Adsorption↗

PubMed1999

The effect of the chemical structure of the phospholipid polymer on fibronectin adsorption and fibroblast adhesion on the gradient phospholipid surface.

The interaction between biocomponents and the polyethylene (PE) surface modified with poly[omega-methacryloyloxyalkyl phosphorylcholine (MAPC)] was considered taking into account the surface characteristics, i.e., density, mobility, and orientation of the poly(MAPC). The PE surface, grafted gradually with the poly(MAPC) was prepared by corona irradiation method. The amount of peroxide produced on the PE surface which was determined with 1,1-diphenyl-2-picryl-hydrazyl, increased with an increase in the energy of the corona. The surface density of the poly(MAPC) was increased with an increase in the amount of the peroxides produced by the corona irradiation. The orientation and mobility of the poly(MAPC) grafted on the PE surface was evaluated with 1,6-diphenyl-1,3,5-hexatriene. The orientation of the poly[6-methacryloyloxyhexyl phosphorylcholine (MHPC)] which has six methylene chains between the phospholipid polar group and the backbone was higher than that of other poly(MAPC)s. The mobility of the poly(MAPC) decreased with an increase in the methylene chain length in the MAPC unit. The fibronectin adsorption on the gradient PE sheet grafted with poly(MAPC) was determined with enzyme-labeled immunoassay. The amount of adsorbed fibronectin on the PE grafted with poly[2-methacryloyloxyethyl phospohorylcholine(MPC)] and poly(MHPC) decreased with an increase in their surface density. Especially, the PE sheet grafted with the poly(MHPC) was effectively reduced compared with other poly(MAPC)s. On the poly[10-methacryloyloxydecyl phosphorylcholine (MDPC)], there is a minimum amount of adsorbed fibronectin. The fibronectin adsorption pattern on the PE sheet grafted with poly(MAPC) was quite different from the chemical structure of the MAPC unit. The human normal diploid fibroblasts (WI-38 cells) were cultured on the gradient PE sheet grafted with poly(MAPC) changing the concentration of seeded WI-38 cells. The adhesion behavior of the WI-38 cells was different depending on the concentration of the seeded WI-38 cells. When the concentration was low, the number of the adherent WI-38 cells had the same tendency as fibronectin adsorption. The gradient PE sheet grafted with the poly(MHPC) effectively reduced WI-38 cells adhesion even when the concentration of the WI-38 cells was high. The biocompatibility of polymer surfaces can be improved by highly oriented phosphorylcholine group.

Adsorption↗

PubMed1999

Combination of EDTA conditioner and phenyl-P/HEMA self-etching primer for bonding to dentin.

OBJECTIVES: The purpose of this study was to examine a new bonding system, combining an ethylenediaminetetraacetic acid (EDTA) conditioner and the 2-methacryloyloxyethyl phenyl phosphoric acid (Phenyl-P)/2-hydroxyethyl methacrylate (HEMA) self-etching primer with a dumbbell-shaped specimen for tensile test. METHODS: Bovine dentin was prepared with a 180-grit paper, conditioned with EDTA, primed with Phenyl-P/HEMA and applied with a light-cured bonding agent, then trimmed to the dumbbell-shaped specimen. After storage in water at 37 degrees C for one day, the tensile bond strength was measured and the hybridization was evaluated by scanning electron microscopy (SEM). RESULTS: When the demineralized dentin with EDTA was primed with 1 wt% Phenyl-P in 30 wt% aqueous HEMA for 10 s, the highest tensile bond strength (22 MPa) was obtained. The SEM views showed that the fractured surface after the tensile test was due to cohesive failure in the cured resin and the 1 micron-hybridized dentin was identified. When the concentration of Phenyl-P and the priming period were increased, tensile bond strength significantly decreased and adhesive failure appeared on the fractured surface. SIGNIFICANCE: Combining the EDTA conditioner and Phenyl-P/HEMA primer afforded high-quality hybridization and good bond strength. This bonding system was promising for bonding resin to human dentin.

Acid Etching, Dental↗

PubMed1999

Multifunctional biocompatible membrane and its application to fabricate a miniaturized glucose sensor with potential for use in vivo.

A multifunctional membrane with biocompatibility, diffusion-limiting effect, and the ability to curtail the responses of an H(2)O(2) electrode to ascorbate and urate was prepared. It was composed of MB, AB, and CTA, where MB is the copolymer of 2-methacryloyloxyethyl phosphorylcholine (MPC) and n-butylmethacrylate (BMA), AB is the copolymer of acrylamide-2-methylpropane sulfonic acid (AMPS) and BMA, CTA is cellulose triacetate. Investigation of the biocompatibility of this membrane showed that, compared with CTA, relatively few platelets bound to it. The membrane was coated onto the working electrode of a needle-type glucose sensor on which immobilized glucose oxidase membrane has been coated. The sensor did not respond to ascorbate and urate at their concentration normally encountered in blood. Its response was not inhibited by metal ions in blood at usual concentration. The sensor exhibited superior thermostability in addition to a rapid response (<90 seconds in batch operation), good reproducibility (RE<5%), good stability (more than 36 hours continuously in heparinized whole blood), and a wide dynamic range (5-650 mg/dl glucose). The sensor was used to determine glucose in serum. The data obtained from the sensor showed good agreement with that from a clinical autoanalyzer (R=0.973).

Journal Article↗

PubMed1999

Improvement of blood compatibility on cellulose hemodialysis membrane: IV. Phospholipid polymer bonded to the membrane surface.

To improve the surface blood compatibility on a cellulose hemodialysis membrane, 2-methacryloyloxyethyl phosphorylcholine (MPC) polymers with a phospholipid polar group were immobilized on the surface through covalent bonding. The MPC polymers had a carboxylic group, which can react with hydroxyl groups on the cellulose membrane, and were synthesized by conventional radical polymerization. The reaction between the MPC polymers and the cellulose membrane was carried out in a heterogeneous system using a condensation reagent. Surface analysis of the modified membrane by X-ray photoelectron spectroscopy revealed the immobilization of the MPC polymer on the surface. The mechanical strength and permeability for a solute of the membrane did not change even after the modification. The modified cellulose membrane was blood-compatible, as determined by the prevention of adhesion, deformation, and aggregation of platelets after contact with platelet-rich plasma. Based on these results, it is concluded that the MPC polymers may be a useful material for improving the blood compatibility of cellulose hemodialysis membranes.

Adsorption↗

PubMed1999

Competitive adsorption between phospholipid and plasma protein on a phospholipid polymer surface.

The competitive adsorption of proteins and phospholipids on omega-methacryloyloxyalkyl phosphorylcholine (MAPC) polymer was evaluated in this study. Albumin, fibrinogen, and dimyrstoyl phosphatidylcholine (DMPC) were used as model components. The amount of DMPC adsorbed on the MAPC polymers increased with an increase in the MAPC unit composition of the polymer. The methylene chain length of the MAPC unit was another factor influencing the DMPC adsorption when the MAPC unit composition of the MAPC polymer was low. The state of albumin and DMPC liposome adsorbed on the 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer was determined by dynamic contact angle (DCA) measurement. The adsorption strength of albumin on the MPC polymer was weaker than that on the poly[n-butyl methacrylate (BMA)], that is, the albumin was detached from the MPC polymer during the rinsing process. On the poly(BMA) surface, no difference in the shape of the DCA loops before and after contact with the DMPC liposomal suspension was observed. Fibrinogen adsorption on the MAPC polymer was detected by gold-colloid labeled immunoassay. The amount of fibrinogen adsorbed on every MAPC polymer surface was reduced by addition of the DMPC liposome in the fibrinogen solution. The number of platelets adhered on the MAPC polymer was also decreased when the DMPC liposome was present in the fibrinogen solution during pretreatment. We concluded that phospholipids were preferentially adsorbed on the MAPC polymer surface compared with plasma protein and that the adsorbed phospholipids played an important role in showing an excellent blood compatibility on the MAPC polymer.

Adsorption↗

PubMed1999

Inhibition of fibroblast cell adhesion on substrate by coating with 2-methacryloyloxyethyl phosphorylcholine polymers.

Fibroblast adhesion and growth behavior were examined on various polymers coated on a poly(ethylene telephthalate) (PET) substrate. The polymers are poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylatel copolymer (PMB)s with different MPC unit compositions, and poly(2-hydroxyethyl methacrylate). Surface analysis by dynamic contact angle measurement revealed that the mobility of the polymer chain on the PET substrate depended on the MPC unit composition, but there was no significant difference between the PMBs with 3-10 mol% MPC units and poly(HEMA). Fibronectin adsorption on the polymer surface from a cell culture medium was determined by immunoassay. The adsorbed fibronection was evenly distrubuted in every polymer, however, the amount was reduced with an increase in the MPC unit composition in the PMB. This result suggested that the MPC unit could weaken the interaction between the polymer surface and proteins. When fibroblast L-929 cells, were cultured on the polymers, the cells adhered and the number of cells increased on not only the hydrophobic poly(BMA) but also on the hydrophilic poly(HEMA). However, the number of cells that adhered on the PMB surface decreased with an increase in the MPC unit composition. This was a result of the fibronectin adsorption behavior. Thus, it could be concluded that since the PMB could suppress cell adhesion proteins e.g. fibronectin, the PMB showed excellent cell adhesive resistance properties.

Adsorption↗

PubMed1999

Effect of phospholipidic boundary lubrication in rigid and compliant hemiarthroplasty models.

Hemiarthroplasty may benefit from materials which produce lower friction and improved boundary lubrication protection during start-up conditions. The purpose of this study was to evaluate the effect of phospholipidic boundary lubrication in both rigid and compliant hemiarthroplasty. An in vitro model was designed to dissociate the relative contribution of implant material compliance and the presence of phospholipid to the overall friction of a hemiarthroplasty contact using bovine articular cartilage. Normal bovine articular cartilage was articulated against four flat materials using reciprocating motion: (a) borosilicate glass: (b) borosilicate glass coated with dipalmitoylphosphatidylcholine (DPPC); (c) polyurethane (PU) elastomer (Tecoflex SG93A, a medical-grade aliphatic thermoplastic PU, Thermedics Incorporated. Woburn, Massachusetts); and (d) surface-coated PU (Tecoflex SG93A substrate coated with lipid-attracting copolymer poly[methacryloyloxyethyl phosphorylcholine (MPC)-co-butyl methacrylate (BMA)]. Tests were conducted in physiologically simulated tribological conditions for a non-conformal point contact. Friction and lubrication analysis was performed using both static and kinetic coefficients of friction mu measured for each group as a function of time for a sliding distance of up to 60 m. Results showed that the inclusion of supplemental phospholipid, DPPC, on a rigid substrate significantly decreased mu in comparison with the control (cartilage-glass). Additionally, removal of phospholipid components from the articular cartilage surface produced a significantly greater start-up mu in comparison with normal cartilage at the test onset. The use of a material with a lower modulus resulted in lower mu for the entire duration of the test. Polyurethane elastomer coated with the lipid-attracting copolymer, poly(MPC-co-BMA), resulted in the lowest frictional response. As seen in this study, the improvement of low-modulus hemiarthroplasty may involve the optimization of chemical modification and incorporation of lipid-attracting MPC copolymers onto compliant materials. However, further tests are warranted to determine whether lipid-attracting MPC copolymers perform as well during long-time, in vivo wear studies.

1,2-Dipalmitoylphosphatidylcholine↗

PubMed1998

Why do phospholipid polymers reduce protein adsorption?

The amount of plasma protein adsorbed on a phospholipid polymer having a 2-methacryloyloxyethyl phosphorylcholine (MPC) moiety was reduced compared to the amount of protein adsorbed onto poly[2-hydroxyethyl methacrylate (HEMA)], poly[n-butyl methacrylate (BMA)], and BMA copolymers with acrylamide (AAm) or N-vinyl pyrrolidone (VPy) moieties having a hydrophilic fraction. To clarify the reason for the reduced protein adsorption on the MPC polymer, the water structure in the hydrated polymer was examined with attention to the free water fraction. Hydration of the polymers occurred when they were immersed in water. The differential scanning calorimetric analysis of these hydrated polymers revealed that the free water fractions in the poly(MPC-co-BMA) and poly(MPC-co-n-dodecyl methacrylate) with a 0.30 MPC mole fraction were above 0.70. On the other hand, the free water fractions in the poly(HEMA), poly(AAm-co-BMA), and poly(VPy-co-BMA) were below 0.42. The conformational change in proteins adsorbed on the MPC polymers and poly(HEMA) were determined using ultraviolet and circular dichroism spectroscopic measurements. Proteins adsorbed on poly(HEMA) changed considerably, but those adsorbed on poly(MPC-co-BMA) with a 0.30 MPC mole fraction differed little from the native state. We concluded from these results that fewer proteins are adsorbed and their original conformation is not changed on polymer surfaces that possess a high free water fraction.

Adsorption↗

PubMed1998

Short-term in vivo evaluation of small-diameter vascular prosthesis composed of segmented poly(etherurethane)/2-methacryloyloxyethyl phosphorylcholine polymer blend.

A small-diameter vascular prosthesis with potential for clinical use was prepared from a Dacron prosthesis coated with nonthrombogenic polymeric materials. As a coating material, segmented poly(etherurethane) (SPU; Tecoflex 60) was blended with a phospholipid polymer, 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer, which has excellent blood compatibility. The Dacron prosthesis, 2 mm in diameter, was immersed in a solution of the SPU/MPC polymer blend and dried to evaporate the solvent. The SPU/MPC polymer prosthesis was nonwater permeable and could be sewn to a natural vessel by a microsurgical technique. The SPU solution was used instead of the SPU/MPC polymer blend solution to prepare a control prosthesis (SPU prosthesis). The SPU/MPC polymer prosthesis and the SPU prosthesis were placed as interposition grafts in rabbit carotid arteries. A massive red thrombus became attached to the surface of the SPU prosthesis as early as 90 min after implantation. In the SPU/MPC polymer prosthesis case, the surface was maintained clear even after 5-day implantation. These observations indicated that the MPC polymer in the SPU could improve the nonthrombogenicity of SPU, and the SPU/MPC polymer blend had potential for preparation of small-diameter vascular prostheses.

Animals↗

PubMed1998

The durability of adhesion to phosphoric acid etched, wet dentin substrates.

OBJECTIVE: The purpose of this study was to investigate the influence of remaining non-resin-impregnated, phosphoric acid demineralized dentin upon the long-term durability of specimens that were wet-bonded to bovine dentin substrates. METHODS: Prepared bovine dentin samples were etched with 65% phosphoric acid then rinsed with water and kept wet during application of 5 wt% 4-methacryloyloxyethyl trimellitate anhydride (4-META) in acetone primer. This was followed by application of a photocured dentin-bonding agent consisting of 4-methacryloyloxyethyl trimellitate anhydride/triethyleneglycol dimethacrylate-camphorquinone/N-phenylglycine (4-META/TEGDMA-CQ/NPG). The tensile bond strength (TBS) of bonded specimens was determined after immersion in 37 degrees C water for various time intervals. Generated data were analyzed for statistical significance by one-way ANOVA and Duncan's New Multiple Range Test (p < 0.05). The dentin side of the tensile-load-fractured specimens was examined under optical and scanning electron microscopes (SEM). RESULTS: TBS decreased from 6.6 +/- 1.0 MPa after 1-day water immersion to 3.4 +/- 1.7 MPa after 1 month of water immersion. After 6 months of water immersion, TBS was found to be 3.9 +/- 0.9 MPa and this decreased to 2.0 +/- 1.0 MPa for specimens immersed in water for 1 year, a statistically significant difference (p < 0.05). Optical microscopic and SEM observations disclosed failure patterns within demineralized, non-resin-impregnated dentin that increased with the period of water immersion. SIGNIFICANCE: The bond durability to wet dentin was poor when demineralized dentin was not resin-impregnated, resulting in exposure of collagen fibrils which hydrolyzed during long periods of water immersion.

Acid Etching, Dental↗

PubMed1998

Performance of adhesive bone cement containing hydroxyapatite particles.

A new acrylic bone cement which can adhere to both bone and prostheses was developed based on a methyl methacrylate (MMA) monomer containing 4-methacryloyloxyethyl trimellitate anhydride (4-META) as adhesion promoting agent. Moreover, hydroxyapatite (HA) particles were introduced into the 4-META cement as a bone compatible filler. The mechanical strengths of an acrylic bone cement without 4-META decreased drastically with an increase in the percentage of HA particles in the cement. However, the mechanical strengths of the HA-containing 4-META cement did not change in the same way as that of the 4-META cement without HA due to adhesion between the cement HA particles and matrix. The HA particles did not affect the adhesion of the 4-META cement to bone and metals. Implantation of the 4-META cement and the HA-containing 4-META cement in animals demonstrated that these cements did not disturb bone ingrowth and the new bone was able to contact the cement directly. The 4-META cements, with and without HA particles, could adhere to bone in vivo.

Biocompatible Materials↗