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Biomedical subjects

N Mitsuhashi

Publications and source records attributed to N Mitsuhashi.

At least 163 records · Page 9Linked to original sources
PubMed1983

[Case report of immunoblastic lymphadenopathy].

A patient with clinical symptoms of general lymphadenopathy, night sweat and weight loss is reported. Laboratory data showed polyclonal hypergammaglobulinemia. Therefore we suspected Immunoblastic Lymphadenopathy (IBL), but could not deny malignant lymphoma because of the histological data. However, we determined that this case was IBL because the use of only steroid therapy was highly efficient.

Adrenal Cortex Hormones↗
PubMed1983

[Case of yolk sac tumor of the anterior mediastinum with superior vena cava syndrome].

The patient was a 21-year-old man with SVC syndrome due to a large anterior mediastinal mass. Following radiotherapy which improved his condition, thoracotomy was performed, however, total resection failed. Despite postoperative re-irradiation, his condition worsened rapidly and he died five months after the onset of his symptoms. The neoplasm was composed of a yolk sac tumor producing alpha-fetoprotein. Yolk sac tumors of the mediastinum are rare and lethal. Recent reports indicate that cis-platinum may be highly effective in germ cell tumors. Therefore, radiotherapy combined with cis-platinum may be improved the survival of patients with yolk sac tumor.

Adult↗
PubMed1981

[Prostaglandin and luteinized unruptured follicles: the follicles of gilts treated with the inhibitors of prostaglandin production (author's transl)].

The inhibitors of prostaglandin (PG) production (indomethacin, etc.) block ovulation. But little is known about how the inhibition of PG production effects cell function in the follicle. This study was done to clarify the morphological changes and steroidogenesis in the follicles treated with the inhibitor. Fifteen gilts treated with PMS and hCG were administered with indomethacin of 7.6 mg/kg (enough for a complete block) 24 hrs. after hCG. The morphological study of the follicles was done by serial section. The levels of estradiol-17 beta, testosterone, 17 alpha OH progesterone and progesterone were estimated by RIA in the follicular fluid, ovarian vein and peripheral blood. A histologic section of the treated follicles revealed the presence of entrapped oocytes surrounded by heavily luteinized granulosa-theca cells. No morphological changes were found in the luteal cells at any stage of the luteal phase except for retention of fluid in the central cavity of the corpus luteum. The life span of the corpus luteum (18 days) was not affected by indomethacin. No differences in steroid levels were detected in the peripheral and ovarian vein blood or in the follicular fluid at any time compared with the control. Inhibition of PG production results in the luteinized unruptured follicle without interference with steroidogenesis.

Animals↗
PubMed1981

Inhibition of superovulation in immature female rats by the antiserum to cyclooxygenase.

In order to investigate involvement of prostaglandins (PGs) in ovulation, we examined whether the antiserum to cyclooxygenase was able to inhibit superovulation in immature female rats as indomethacin did. The antiserum to cyclooxygenase was raised in rabbits using as antigen the solubilized cyclooxygenase extracted and purified from bovine seminal vesicles. The in vivo administration of the antiserum inhibited superovulation in female rats dose-dependently at doses of 0.1, 0.2 and 0.4 ml but did not affect production of progesterone. Inhibition of cyclooxygenase in the follicular cells due to the antiserum causing no effect on steroidogenesis was observed in organ cultures of the follicles excised from rats primed with PMS-hCG and the antiserum. In contrast to this, no inhibition of cyclooxygenase was detected upon adding the antiserum to the culture medium. The present study showed PG is as indispensable a factor in ovulation as are the steroids.

Animals↗
PubMed1981

Bioconversion of arachidonic acid to prostaglandins by Graafian follicles and stroma from the human ovary.

In order to study production of prostaglandins (PGs) by human pre-ovulatory ovarian tissue, bioconversion of 14C-arachidonic acid to PGs was examined using homogenates of human Graafian follicles and stromal tissues fully stimulated by endogenous follicle-stimulating hormone in the regular menstrual cycle. Among the radioactive metabolites extracted, PGE2 and 6-keto PGF1 alpha were purified and identified by silicic acid column-, thin layer-, reversed phase partition chromatographies and radio-gas chromatography. The follicular tissue was shown to convert 14C-arachidonic acid to PGE2 at a conversion rate of 0.34% but not to 6-keto PGF1 alpha. On the other hand, the stromal tissue was demonstrated to produce not only 14C-labeled PGE2 at a conversion of 0.09% but mainly 14C-labelled 6-keto PGF1 alpha at 0.26%.

6-Ketoprostaglandin F1 alpha↗
PubMed1981

Biosynthesis of prostaglandins from arachidonic acid by the rat ovary.

Biosynthesis of prostaglandins (PGs) from 14C-arachidonic acid was studied using homogenates of the ovaries from immature rats. In ascending order of metabolizing potency were, the ovaries from untreated rats, from rats treated with pregnant mare's serum gonadotropin (PMS), and from PMS-human chorionic gonadotropin (hCG) treated rats. Among the radioactive metabolites extracted, PGE2 and 6-keto PGF1 were purified and identified by silicic acid column-, thin layer-, reversed phase partition chromatographies, and radiogaschromatography. Production of PGE2 and 6-keto PGF1 was observed in homogenates of the ovaries of intact and PMS-hCG treated rats at conversion rates of 0.72; 0.43% and 7.62; 2.31%, but not by PMS treated rat ovaries. Treatment with PMS-hCG activated metabolism of arachidonic acid into radioactive metabolites including PGE2 and 6-keto PGF1 to a large extent. Accordingly, it is concluded that luteinizing hormone and hCG play a significant role in the biosynthesis of PGs by the rat ovarian follicle.

6-Ketoprostaglandin F1 alpha↗
PubMed1981

[Studies on the mechanism and the significance of prostaglandin biosynthesis by the ovary --ovulation block by the indomethacin and incubation of the follicle (author's transl)].

The involvement of prostaglandins (PGs) in the process of ovulation was studied. 1) The superovulation of immature rat was blocked by indomethacin dose dependently and which was reversed by the administration of PGs. 2) Preovulatory increase of PGs was observed in the ovaries of rats and follicular fluid of gilts. 3) Immature rat follicle synthesised PGE, PGF, and 6ketoPGF1 alpha in organ culture. This synthesis was activated by hCG and suppressed by actinomycin D and puromycin. 4) Production of PGs by gilt and human follicles was observed and which was activated by hCG.

6-Ketoprostaglandin F1 alpha↗
PubMed1981

[Studies on the mechanism and the significance of prostaglandin biosynthesis by the ovary--conversion of 14C-arachidonic acid to prostaglandins (author's transl)].

Biosynthesis of prostaglandins (PGs) from 14C-arachidonic acid was studied using homogenates of the ovaries from rats, gilts and human. Among the radioactive metabolites extracted, PGE2 and 6 keto PGF1 alpha were purified and identified by silicic acid column-, thin layers-, reversed phase partition chromatographies, and radiogaschromatography. Treatment with PMS-hCG activated metabolism of arachidonic acid into radioactive metabolites including PGE2 and 6 keto PGF1 alpha to a large extent. Therefore it is concluded that LH or (hCG) activate the PG synthetase system through the induction of synthetase itself and/or a stimulatory factor.

Animals↗
PubMed1980

Prostaglandin F2 alpha metabolites in plasma and urine during pseudopregnancy in the rabbit.

Two prostaglandin (PG) F2 alpha metabolites, 13,14-dihydro-15-keto PGF2 alpha (dhkPGF2 alpha) in blood and 5 alpha, 7 alpha-dihydroxy-11-keto tetranorprostane 1,16-dioic acid (PGF2 alpha UM) in urine, were measured in the pseudopregnant rabbit by radioimmunoassay. During pseudopregnancy, marked increases in the two PGF2 alpha metabolites were demonstrated at about the time of luteal regression. Hysterectomy and intrauterine administration of indomethacin prolonged the life span of corpora lutea in the pseudopregnant rabbit by 8 and 5 days, respectively, with no elevation of PGF2 alpha UM excretion. The findings indicate that PGF2 alpha is a uterine luteolysin in the pseudopregnant rabbit, as in other animal species.

Animals↗
PubMed1980

Identification of drug resistance (R) plasmids from catheter urine.

We isolated bacterial strains from catheter urine of patients with underlying diseases and investigated their resistance to various drugs. It was found that Serratia marcescens strains were most frequently isolated, from which R plasmids were identified at a high frequency (70%). According to the resistance patterns of R plasmids, prevalence of multiply resistant S. marcescens strains was concluded by selection of the organism according to drugs used and by infection with the organism, and the spread of R plasmids encoding multiple resistance.

Bacteriuria↗