Biomedical subjects

N Liappis

Publications and source records attributed to N Liappis.

At least 91 records · Page 5Linked to original sources

PubMedDate not supplied

[Comparison of free amino acids in capillary and venous blood serum of children and adolescents with phenylketonuria and leucinosis].

Concentrations of free amino acids were determined quantitatively by ion exchange column chromatography in serum from capillary and venous blood of children and adolescents with phenylketonuria (n = 38) and leucinosis (n = 3). Serum was deproteinised by 5% sulphosalicylic acid (1:1, v/v). The results showed with exception of aspartic acid, glutamine and glutamic acid a very close correlation. The correlation coefficients ranged from 0.849 to 0.996. It is concluded that serum from capillary blood can be used for screening of amino acid metabolism.

Adolescent↗

PubMedDate not supplied

[Comparison of the ferritin and iron concentration in capillary and venous blood serum of children, adolescents and adults].

The ferritin and iron concentration were compared in sera from capillary and venous blood (n = 52). Ferritin was determined using a luminescence-enhanced enzyme immunoassay and iron by the ferrozine method. The results showed a very good correlation and a linear relationship for ferritin (r = 0.999) but not for iron (r = 0.855). It is concluded that capillary blood serum can be used only for the determination of ferritin.

Adolescent↗

PubMedDate not supplied

[Reference values for blood levels of free thyroxine, free triiodothyronine and thyroxine-binding globulins in euthyroid children. Method: luminescence-enhanced enzyme immunoassay].

Concentrations of free thyroxine (n = 296, male 164, female = 132), free triiodothyronine (n = 273, male 156, female = 117) and thyroxine-binding globulin (n = 273, male 156, female = 117) were determined using the luminescence enhanced enzyme immunoassay method in blood-serum of euthyroid children. Beyond the 30, day of life no significant differences were found in different age groups for the free thyroxine and beyond the 2, day of life for the free triiodothyronine concentration. The free thyroxine values of the infants in the range of 15-39 pg/ml decreased to the range of 9-18.5 pg/ml and the free triiodothyronine values decreased from 3.4-9.3 pg/ml to the range of 2.8-6.5 pg/ml. The values for thyroxine-binding globulin increased in the first month of life up to the range of 18-35 mg/l and decreased afterwards continuously to the range of 14.5-26.5 mg/l.

Adolescent↗

PubMedDate not supplied

[Microalbuminuria in children and adolescents with and without Type-I diabetes mellitus (IDDM)].

In 73 healthy (group I) and 32 children and juveniles with insulin dependent diabetes mellitus (IDDM, group II) urinary albumin excretion is determined by radioimmunoassay (RIA). In both groups albumin excretion is observed in every urine sample when measured by RIA (mean +/- SD: group I: 7-19 h: 5.17 +/- 5.28 mg, 19-7 h: 3.86 +/- 4.00 mg, 24 h: 9.03 +/- 8.60 mg; group II: 7-19 h: 6.68 +/- 6.86 mg, 19-7 h: 3.46 +/- 2.82 mg, 24 h: 10.13 +/- 9.25 mg). No significant difference is detected between the values of the two groups. However in diabetic patients a significant difference is observed between diurnal and nocturnal urinary albumin excretion. Microalbuminuria is defined as an albumin excretion above 30 mg/d and is present in 6.9% of the values in group I and in 3.1% in group II. The physiological limits of microalbuminuria in children and juveniles compared to adults and several methods of urine sampling are discussed.

Adolescent↗

PubMedDate not supplied

[Serum fructosamine in healthy and diabetic children and adolescents].

The determination of fructosamine in serum of healthy newborns, children and adolescents by a new colorimetric method leads to an age-dependent reference range. Correction of the values for total protein yields a median of the results, which is not dependent on age and the upper limit of the reference range is not significantly different from that of healthy adults; this does not hold true for referral to albumin. As the half-life time of glycated serum proteins is shorter (mean 20 days) than that of hemoglobin A1c, fructosamine can provide useful additional informations about the diabetic control of children and adolescents (medium-term record of blood glucose).

Adolescent↗

PubMedDate not supplied

[Comparative determination of microalbuminuria using radioimmunoassay and kinetic nephelometry in children and adolescent with and without Type-I diabetes mellitus (IDDM)].

In 73 healthy (group I) and 32 children and juveniles with insulin dependent diabetes mellitus (IDDM, group II) urinary albumin excretion is determined by radioimmunoassay (RIA) and kinetic nephelometry. Intention of the study is to examine, if the kinetic nephelometry is--as observed in adults--a suitable method also in children and juveniles to detect microalbuminuria (greater than 30 mg/d). In both groups albumin excretion is observed in every urine sample when measured by RIA. Because of it's higher threshold kinetic nephelometry detects albumin excretion only in a part of the urine samples. The correlation between the two methods is very high (r = 0.905, p less than 0.001, n = 174). So kinetic nephelometry is not suitable to determine reference values. But as a faster and possibly more specific method than RIA nephelometry is a very effective way for a screening of microalbuminuria also in children and juveniles.

Adolescent↗

PubMedDate not supplied

[Reference values of the ferritin concentration in the serum in children].

Concentrations of ferritin were determined using the luminescence enhanced enzyme immunoassay method in serum of 272 (male = 148, female = 124) "healthy" children. The ferritin concentration in children is age dependent. The newborn values (140.1-673.8 micrograms/l, 95% confidence interval) decrease until the end of the first year and stabilize thereafter in a sex specific fashion: male means = 31.90 micrograms/l (95% confidence interval: 14.0-72.2 micrograms/l), female means = 39.23 micrograms/l (95% confidence interval: 16.1-79.9 micrograms/l).

Adolescent↗

PubMedDate not supplied

[Reference values of fasting serum levels of free amino acids in children].

Concentrations of free amino acids were determined quantitatively by ion exchange column chromatography in serum from the fasting blood of 352 (male = 177, female = 175) "healthy" infants, children and adolescents. Serum was deproteinised by 5% sulphosalicylic acid (1:1, v/v). The results -95% confidence range-are presented age-dependent (groups: up to 1 month, 1-24 months, 2-6 years, 6-10 years, 10-14 years) and sex-dependent in table 4 and 5.

Adolescent↗

PubMedDate not supplied

[Age-specific and sex-specific reference values for the excretion of free amino acids in the 24-hour urine of children].

Free amino acids were determined quantitatively by ion exchange column chromatography in 24 h urine of 354 (male = 187, female = 167) "healthy" infants, children and adolescents. Urine was deproteinised by 5% sulphosalicylic acid (1:1, v/v). The results - 95% confidence range - are presented age-dependent (groups: up to 1 month, 1-24 months, 2-6 years, 6-10 years, 10-14 years) and sex-dependent in table 3 and 4.

Adolescent↗

PubMedDate not supplied

[Determination of C-reactive protein in serum using a fluorescence polarization immunoassay--comparison with the method of kinetic nephelometry (KNM)].

The determination of CRP in serum was performed by the fluorescence polarization immunoassay (FPIA) and the kinetic nephelometry (KNM). Both methods showed a good precision and accuracy. The investigation was carried out in 190 sera with a CRP concentration up to 49 mg/dl. The comparison of the two methods in 100 sera with a CRP concentration between 0.6 and 5 mg/dl showed a good correlation (correlation coefficient r = 0.9753). Close correlation (correlation coefficient r = 0.9947) was also found in 90 sera with a CRP concentration between 5 and 49 mg/dl. Discussion of the FPIA and KNM methods.

C-Reactive Protein↗

PubMedDate not supplied

[Reference values for the concentration of free thyroxine and free triiodothyronine in the serum of euthyroid children].

Concentrations of free thyroxine (n = 341, male = 205, female = 136) and free triiodothyronine (n = 318, male = 198, female = 120) were determined using the radioimmunoassay method in blood serum of euthyroid children. Beyond the 30. day of life no significant differences were found in different age groups for the free thyroxine and free triiodothyronine concentration. The free thyroxine values of the infants in the range of 16.5-19.5 pg/ml decreased after the 30. day of life to the range of 15-17.5 pg/ml. In opposition to this trend free triiodothyronine values increased after the 30. day of life from the range of 2.5-3.3 pg/ml up to the range of 4.7-5.5 pg/ml.

Adolescent↗

PubMedDate not supplied

[Free amino acids in the saliva of children with phenylketonuria].

UNLABELLED: Free amino acids were determined quantitatively in saliva of 23 children with phenylketonuria (male = 11, female = 12) (ages 6-17 years). Saliva was deproteinised by adding an equal volume of 5% sulphosalicylic acid and the amino acids were separated by ion exchange column chromatography. RESULTS: The comparison of the amino acid concentrations in saliva of phenylketonuric children with healthy children (male = 34, female = 31) (ages 6-13 years) showed, that vast majority of amino acids - taurine, serine, glutamine, glycine, alanine, citrulline, a-aminobutyric acid, valine, isoleucine, leucine, tyrosine, phenylalanine, ornithine, lysine, delta-aminovaleric acid - were excreted significantly lower in saliva of phenylketonuric children.

Adolescent↗

PubMedDate not supplied

[Determining the methotrexate concentration in serum with the fluorescence polarization immunoassay (FPIA)--a comparison with the enzyme immunoassay (EMIT)].

The determination of methotrexate in serum was performed by the fluorescence polarization immunoassay (FPIA) and the enzyme immunoassay (EMIT). Both methods showed a good precision and accuracy. The investigation was carried out in 138 sera with a methotrexate concentration up to 1,400 mumol/l. The comparison of the two methods in 100 sera with a methotrexate concentration up to 5 mumol/l showed a good correlation (correlation coefficient r = 0.9798). Close correlation (correlation coefficient r = 0.9845) was also found in 38 sera with a methotrexate concentration between 5 and 1,400 mumol/l. Discussion of the FPIA and EMIT methods.

Child↗

PubMedDate not supplied

[Reference values of beta 2 microglobulin concentrations in the serum of children].

Concentrations of beta 2-microglobulin were determined using a competitive enzyme immunoassay in blood serum of 271 "healthy" children (male = 156, female = 115). The concentration of beta 2-microglobulin in children is age dependent. The values of the newborn 2.84-3.38 mg/l (95% confidence interval) decrease till the first year and stabilize later on between 1.48-1.56 mg/l (95% confidence interval).

Adolescent↗

PubMedDate not supplied

[Determination of bilirubin in capillary plasma by a direct photometric method (DPM, bilirubinometer) and the chemical determination of bilirubin in the bilirubin determination (2,5-dichlorophenyldiazonium method) in serum of venous blood samples].

The determination of bilirubin in serum was performed by the 2.5-dichlorphenyldiazonium method (DPD) and in capillary plasma by the direct photometric method (DPM). Both methods showed a good precision and accuracy. The investigation was carried out in 135 samples with a bilirubin concentration up to 25 mg/dl. The comparison of the two methods in 62 samples with a bilirubin concentration up to 10 mg/dl showed a correlation coefficient of r = 0.862 and in 73 samples with a bilirubin concentration between 10 and 25 mg/dl a correlation coefficient of r = 0.893. In 29 cases (21.5%) we found differences between the two methods of 1.5-4.0 mg/dl. Most of them were in the critical higher range. Discussion of the DPD and DPM methods.

Bilirubin↗

PubMedDate not supplied

[Reference values for the concentrations of triiodothyronine, thyroxine and thyrotropin in the blood serum of euthyroid children. Method: luminescence-enhanced enzyme immunoassay].

Concentrations of triiodothyronine, thyroxine and thyrotropin were determined using the luminescence enhanced enzyme immunoassay method in blood serum of 343 (male = 197, female = 146) euthyroid children. Beyond the newborn age no significant difference was found for the thyrotropin concentration. Compilation of the values resulted in a physiological thyrotropin concentration of 1.73 +/- 0.81 (1s) microU/ml for infants, children and juveniles. In opposition to these results the thyroxine and triiodothyronine concentration showed an age-specific dependence. The thyroxine and triiodothyronine concentrations (means +/- 1s) decreased continuously from the infant age (TT4: 182.1 +/- 37.7 ng/ml, 234.9 +/- 48.6 nmol/l, TT3: 1.77 +/- 0.57 ng/ml, 2.73 +/- 0.88 nmol/l) to the juvenile age (TT4: 77.9 +/- 14.3 ng/ml, 100.5 +/- 18.4 nmol/l, TT3: 1.25 +/- 0.31 ng/ml, 1.93 +/- 0.48 nmol/l). The luminescence enhanced enzyme immunoassay technique is suitable for use in the routine thyroid laboratory.

Adolescent↗

PubMedDate not supplied

[Immunoglobulin administration as infection prevention in oncologic patients].

Children receiving cytotoxic drugs were given 7 S IgG or placebo before the period of neutropenia (less than 500/mm3 neutrophils) in a double-blind study. Though the IgG levels differed significantly, fever as the most reliable sign of infection occurred not less often than in the placebo-group. There is no indication for the routinely prophylactic administration of immunoglobulins to oncological patients. The therapeutic benefit of immunoglobulin substitution in cancer patients needs verification.

Adolescent↗

PubMedDate not supplied

[Behavior of the concentration of triiodothyronine, thyroxine, thyrotropin and thyroxine-binding globulin in the serum of children with nephrotic syndrome].

Concentrations of triiodothyronine, thyroxine, thyrotropin, thyroxine-binding globulin, urea, creatinine, cholesterol and total protein were determined in serum of six children (ages from 4 to 16 years) with nephrotic syndrome undergoing therapy. The results showed that with decreasing concentration of serum protein and increasing concentration of cholesterol the concentrations of thyroxine and thyroxine-binding globulin decreased whereas the concentration of thyrotropin was elevated. Improvement in the concentration of serum protein lead to an improvement of serum levels of thyroid hormones and thyroxine-binding globulin; concentrations of thyrotropin and cholesterol returned to values close to normal. Although triiodothyronine was decreased slightly it remained in the normal range thus preventing overt hypothyroidism.

Adolescent↗