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Biomedical subjects

N Kondo

Publications and source records attributed to N Kondo.

At least 541 records · Page 30Linked to original sources
PubMed1984

Rat lymphoid cell lines with human T cell leukemia virus production. I. Biological and serological characterization.

Cocultivation of spleen cells, lymph node cells, and thymocytes of female Wistar-King-Aptekman rats with short-term cultured male adult T cell leukemia (ATL) cells in the presence of 5-bromo-2'-deoxyuridine (BrdUrd) resulted in the establishment of rat lymphoid cell lines, TARS-1, TARL-2, and TART-1. Cytogenetic analysis of the three cell lines showed a female rat karyotype with 42 chromosomes. The surface phenotypes of TARS-1 and TART-1 were those of rat T cells. TARL-2 was only positive for rat Ia and leukocyte common antigens. The cell lines continuously produced a type C retrovirus, human T cell leukemia virus (HTLV) and expressed ATL-associated antigens. TARS-1 and TART-1, but not TARL-2 were transplantable into newborn syngeneic rats and nude mice. These results strongly indicate that HTLV not only immortalizes, but also transforms rat T cells in vitro. Adult rats immunized with either TARS-1 or TARL-2 produced antibodies specific for HTLV. The biochemical analysis of the antigens that reacted with rat sera revealed that they are the two HTLV-specific polypeptides, p24 and p28.

Aged↗
PubMed1984

Molecular homology among the structural proteins of densonucleosis virus from silkworm, Bombyx mori.

Similarities among the four structural proteins of Bombyx densonucleosis virus (DNV) were examined by four independent techniques, peptide mapping, immunodiffusion tests, amino acid analysis and enzyme linked immunosorbent (ELISA) technique. The peptide maps produced by Staphylococcus aureus V8 protease, or chymotrypsin indicated the existence of sequence homology among the proteins. The results of immunodiffusion tests revealed that these four structural proteins share common antigens. The amino acid compositions of these proteins were also very similar to each other. However, the amount of amino acid residues (e.g., serine) in VP3 was not always sufficient to account for the amount found in the smaller structural protein, VP2. These results indicate that the four structural proteins of Bombyx DNV probably originate, at least partially, from a common DNA sequence, and that VP2 is not a direct cleavage product of VP3.

Amino Acids↗
PubMed1984

Lupus nephritis. Clinicopathology and immunopathology of 80 biopsy cases.

The clinicopathology and immunopathology of 80 cases of lupus nephritis in a total of 1,187 biopsies were presented. Histologically, they consisted of 11 cases of mesangial lupus nephritis (13.8%), 51 cases of diffuse proliferative lupus nephritis (63.7%), and 18 cases of membranous lupus nephritis (22.5%). No cases of focal proliferative type, minimal changes or end-stage kidneys were included. Ten cases were male and they had no qualitative and quantitative differences with female ones in any respects. The microtubular structures were found in glomerular endothelial cells with a 70% frequency and in the interstitial endothelial cells with a 46% frequency. In the latter case, microtubular structures tended to be localized in the perinuclear space as well as in the endoplasmic reticulum. Spherical microparticles were found in 46% of all cases and the incidences were significantly higher in the membranous type. Organized deposits with straight parallel microfilaments were found only in a single case. Electron-dense deposits with fingerprint pattern were not seen. Immunohistochemically, various immunoglobulins, complement components, and fibrinogen were found deposited more frequently in diffuse proliferative lupus nephritis. IgG was the predominant immunoglobulin deposited in capillary loops in membranous types, and IgA in the mesangium in mesangial types. Extraglomerular deposition of immunoglobulins and complements were found in 26.3% of all cases.

Complement Activating Enzymes↗
PubMed1984

[A case study with bladder metastasis of renal cell carcinoma and stomach cancer].

A 64-year-old woman received nephrectomy and lymph expurgation surgery for renal cell carcinoma on Jury 1, 1981. The pathologic diagnosis was adenocarcinoma of the clear cell type at Robson's stage 2. She next visited the Department of Gastroenterology complaining of stomach discomfort on November 5, 1981. Stomach cancer of Borrmann's type IV was identified in the lesser gastric curvature, but only biopsy was performed because it was inoperable. The pathologic diagnosis was undifferentiated adenocarcinoma. On January 23, 1982, there was microscopic hematuria. A cystoscopic examination revealed one soy bean-sized, smooth, pedicle tumor to which coagula were partially adhered in the center of the triangular region. After TUR-Bt performed on March 3 the pathologic diagnosis was adenocarcinoma of the clear cell type with no submucosal infiltration. Based on these findings, the patient was diagnosed as having suffered metastasis of renal cell carcinoma to the bladder. She died of bleeding from stomach cancer on June 15. Based on the fact that the tumor was localized in the bladder mucosa, implantation through the urinary tract was strongly suspected as the metastatic route of the renal cell carcinoma to the bladder.

Adenocarcinoma↗
PubMed1983

Competence of B cells for T-cell help in pokeweed mitogen-induced immunoglobulin production.

Pokeweed mitogen (PWM)-induced polyclonal immunoglobulin (Ig) production of B cells is highly reproducible and the response has been shown to be dependent on T cells. We investigated whether or not human helper T cells are able to collaborate with B cells in PWM-induced polyclonal Ig production across allogeneic barriers, using T and B cells obtained from healthy individuals and a(hypo)-gammaglobulinemia patients. In results, it has become clear that the B-cell competence is required for T-cell help among healthy individuals and between healthy and a(hypo)-gammaglobulinemic individuals. These phenomena were associated with suppressor or killer T cells rather than HLA-A, -B, -C, and -DR antigens. They also imply that in order to investigate the T-cell helper activities of a(hypo)-gammaglobulinemia patients their T cells should be tested in cocultures not only with B cells of the third party individuals but also with B cells of patient family members.

Agammaglobulinemia↗
PubMed1983

Effect of prolonged cold storage on the mechanical activity of rabbit atria.

Even after 21 days of cold storage at 2 degrees C, rabbit atria still showed a detectable response only to isoproterenol while other cardiostimulants (norepinephrine, ouabain, anthopleurin-A and nicotine) failed to activate tissues stored up to 14 days. The specific fluorescence of catecholamines disappeared from the tissue after 5 days of cold storage. The maximum decrease in oxygen consumption and tissue catecholamine content was observed after 14 days and a concomitant decrease in tissue ATP content, Na+-K+, Ca2+ and Mg2+-ATPases was nearly maximal after 7 days of cold storage. The cold storage had no apparent effect on the ultrastructures of myocardial cells except a disappearance in glycogen granules.

Adenosine Triphosphate↗
PubMed1983

The effect of creatine on the developed tension and metabolic kinetics of isolated rabbit atria after prolonged cold storage.

During 14 days of cold storage the mechanical and metabolic functions and sarcolemmal enzyme activities were markedly decreased. Creatine significantly suppressed such decrease in oxygen consumption and preserved the ability to reproduce high energy phosphates from low temperature, resulted in recovery of mechanical activity by rewarming. However, cation transport enzymes, Na+, K+- and Ca2+-ATPase, were not affected, These results suggest the protective action of creatine on mammalian myocardium under cold storage.

Adenosine Triphosphatases↗
PubMed1983

Effects of amrinone on the transmembrane action potential of rabbit sinus node pacemaker cells.

Effects of amrinone on the membrane action potential and spontaneous activity were investigated in sinus node pacemaker cells of rabbit heart by use of microelectrode techniques. Amrinone (1 X 10(-4) M to 6 X 10(-6) M) caused a shortening of cycle length of spontaneous firing (SPCL) accompanied by an increase in the maximum upstroke velocity at phase O (Vmax) and amplitude of action potential (AAP), while it did not affect the maximum diastolic potential (MDP). All the effects of amrinone on sinus node pacemaker cells were markedly attenuated or abolished in a low calcium medium (Ca+ 0.1 mM or 0.3 mM) or in the presence of the slow channel blocking agent, verapamil (5 X 10(-7) M, 2 X 10(-6) M). The effects of amrinone were not antagonized by the beta-adrenoceptor blocking agent, pindolol (2 X 10(-7) M). These results indicate that amrinone has an intrinsic effect on sinus node pacemaker cells, increasing their spontaneous firing activity. It is also assumed that the effects of amrinone on sinus node cells are probably mediated by an augmentation of the slow calcium and/or sodium inward current through the cell membrane.

Action Potentials↗
PubMed1983

[Experimental chemotherapy of human testicular carcinoma transplanted in nude mice].

The testicular carcinoma serially transplanted in nude mice with BALB/c genetic background was used for experimental chemotherapy. A stable growth and a high production of alpha-fetoprotein (AFP) were observed in this tumor line. The effect and side effect of Cis-platinum (CDDP) and other anticancer agents on this tumor line in nude mice were studied by the chemotherapy with single administration of CDDP 2 mg/kg, 4 mg/kg, 6 mg/kg and 8 mg/kg, and the combination chemotherapy with CDDP, Vinblastine (VBL) 0.1 mg/kg, Bleomycin (BLM) 0.5 mg/kg and Cyclophosphamide (CTX) 2 mg/kg. The body weight of the tumor bearing nude mice, the tumor size (length X breadth) and serum AFP level were measured every week up to 10 weeks after inoculation of the tumor mass. Six weeks after administration of these anticancer agents, the tumor mass was removed out and examined histologicaly. The effects of CDDP and other anticancer agents were observed as inhibition of the tumor growth and regression of the tumor mass. In the groups treated by the combination chemotherapy with either CDDP + VBL + BLM or CDDP + VBL + CTX, the most remarkable inhibition and regression were observed. The AFP levels were remarkably decreased in contrast with those of the control group. The changes of serum AFP levels were reflected in the tumor growth. The serum AFP levels fell down to normal level, however, the tumor mass was clearly recognized. The tumor tissue was damaged histologicaly by the single administration of CDDP. The most remarkable change was shown in the group treated by the combination chemotherapy CDDP 4mg/kg + VBL + BLM. The tumor cells were arranged one or two layers like the epithelium. This histological findings suggested that the malignant tumor could be differentiated to benign tumor. The side effect of CDDP and other anticancer agents was observed as a loss of weight. All of mice treated by the single administration at a dose of CDDP 6 mg/kg and 8 mg/kg died of the side effect of CDDP.

Animals↗
PubMed1983

Dimorphism of sister chromatid exchange in Bloom's syndrome B- and T-cell lines transformed with Epstein-Barr and adult T-cell leukemia viruses.

The present study describes the establishment of both B- and T-cell lines from the peripheral blood cells of two Bloom's syndrome (BS) patients and one healthy female by using Epstein-Barr (EBV) and adult T-cell leukemia viruses (ATLV). The cell lines from normal and BS subjects exhibited cell surface markers compatible with B- and T-cell origin; in addition, the BS B- and T-cell lines retained the original cytogenetic characteristics of the syndrome. Even though phytohemagglutinin-stimulated BS lymphocytes from the two BS patients studied all showed high levels of sister chromatid exchange (SCE), the established BS B-lines with EBV yielded two separate lines each, i.e., one with increased SCE and another with normal levels of SCE; also, one of the BS T-lines retained high SCE levels in 100% of the cells, whereas the other BS T-line contained two populations, one with high SCE (70%) and the other with normal SCE levels (30%), at a relatively constant frequency over a period of 6 months. Neither EBV nor ATLV caused a significant increase in chromosome instability in the established lines compared to fresh lymphocytes. Reinfection of the BS B- and T-cell lines with EBV or ATLV did not alter the SCE or karyotypes. These results strongly suggest that BS patients have two populations in vivo, one with high and another with normal levels of SCE, at least in the lymphoid cell system.

Adolescent↗
PubMed1983

Transformation of Bloom syndrome T-lymphocytes by cocultivation with a lethally irradiated human T-cell line carrying type C virus particles.

The present study describes the establishment of T-cell lines from the peripheral blood of two Bloom syndrome (BS) patients and one healthy female by co-cultivation with a lethally irradiated human T-cell line (MT-2) carrying adult T-cell leukemia (ATL) virus (ATLV). The cell lines from normal and BS subjects exhibited cell surface markers compatible with T-cell origin, and BS T-cell lines retained the original cytogenetic characteristics of the syndrome. Even though phytohemagglutinin-stimulated BS lymphocytes from the two BS patients studied all showed high levels of sister chromatid exchange (SCE), one of the BS T-lines retained the high SCE level in 100% of the cells and the other BS T-line contained two populations, one with high SCE (70%) and the other with normal SCE (30%), at a relatively constant frequency over 6 months. Transformation of normal and BS cells by cocultivation with MT-2, which carries ATLV, did not cause karyotypic changes over 6 months. ATLV infection, chromosome instability, karyotypic abnormality and SCE in BS T-lymphocytes are also discussed.

Bloom Syndrome↗
PubMed1983

[The effect of prostaglandin F2 alpha on the gastrointestinal movement after urological surgery].

To facilitate postoperative flatus, Prostaglandin F2 alpha (PGF2 alpha) was given intravenously to 23 patients who underwent urological operations. The patients were 14 males and 6 females aged from 20 to 77 years old. Patients with hypertension or cardiovascular disease were not included. Twelve operations were performed under general anesthesia, and 8 under epidural anesthesia. Thirteen operations were performed for the upper urinary tract or adrenal gland, and 5 were for the lower urinary tract. In 2 cases, the peritoneal cavity was opened and operations were performed on the intestines. PGF2 alpha 2000 micrograms was added to the postoperative drip infusion and administered in 2 to 3 hours. Until the first flatus was recognized, PGF2 alpha was given once a day in the same manner. Twenty-six patients, 10 of whom were given either vagostigmine or pantothen postoperatively, served as the control group. PGF2 alpha accelerated the postoperative flatus by 8.7 hours (mean) compared with the control group, but it was not significant. The onset of flatus was significantly promoted under epidural anesthesia. Gastrointestinal movement tended to be facilitated in the PGF2 alpha group after lower urinary tract surgery and in the patients over 50 years old. Three patients complained of severe abdominal pain as a side effect; and, injection of PGF2 alpha was stopped. In 7 patients, mild stomachache , vascular pain, nausea, vomiting or elevation of blood pressure were observed.

Adult↗
PubMed1983

[A case of ovarian dermoid cyst with bladder perforation].

A 60-year-old female presented with the main complaints of hematuria, pain upon urination, and pollakiuria. Simple abdominal X-rays revealed a dumb bell-shaped calculus shadow, and a calculus was detected in the right posterior wall by cystoscopy. At surgery, a left ovarian dermoid cyst accompanied by a calculus that was adhesive and perforating in the right posterior wall of bladder was detected. Twenty-eight cases of ovarian dermoid cyst with bladder perforation including this case are reported.

Calcinosis↗
PubMed1983

[Photocystoscopy: experience with a new telescope and polaroid camera].

The ability to photograph the interior of the bladder accurately just by pressing a button has great clinical merit. This approach, which has become a basic method in gastrointestinal endoscopy, is now possible in cystoscopy. We will call this technique which uses a newly designed telescope, Olympus A 3405 and Polaroid Instant Endocamera EC-3 with Polaroid High Speed Color Land Film 600, photocystoscopy . The telescope used in photocystoscopy has more improved resolution and homogeneous brightness through its field of view than the conventional telescope, Olympus O 3405. The photographic image of the bladder interior taken by the Polaroid camera has a good color and fine resolution but its size which is 2.5 cm in diameter is too small for clinical use. Color instantographs by the Polaroid camera are most appropriate for use in photocystoscopy , but many devices and improvements are necessary before it can be used routinely.

Cystoscopes↗
PubMed1981

Successful resection of an aortic arch aneurysm using a temporary trifurcation bypass graft.

Resection of a syphilitic aortic arch aneurysm in a 62-year-old woman was accomplished using a trifurcation temporary bypass system. The bifurcation graft was sutured end-to-side to the ascending thoracic aorta, to the brachiocephalic trunk and to the left common carotid artery, respectively. The attached third limb end was anastomosed end-to-side to the infrarenal abdominal aorta. This technique enabled a large shunt into the abdominal aorta. Occlusion of the iliac arteries proved to be effective in coping with hypotension while attending to the bypass and the volume replacement. The post-operative recovery was uneventful and the patient has remained well after 43 months.

Aorta, Thoracic↗