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Biomedical subjects

N Kaneko

Publications and source records attributed to N Kaneko.

At least 127 records · Page 7Linked to original sources
PubMed1984

Detection of bovine coronavirus in feces by reversed passive hemagglutination.

A reversed passive hemagglutination (RPHA) method was developed for the detection of bovine coronavirus in fecal specimens. Sheep erythrocytes fixed with glutaraldehyde, and then treated with tannic acid were coated with anti-bovine coronavirus rabbit antibodies purified by affinity chromatography using bovine coronavirus linked to Sepharose 4B. The RPHA test was carried out by a microtiter method. Erythrocytes coated with purified specific antibodies were agglutinated by bovine coronavirus, but not by bovine rotavirus or enterovirus. The reaction was inhibited by antiserum to bovine coronavirus, confirming the specificity of the reaction. The RPHA test detected bovine coronavirus in 13 of 22 fecal specimens (59 per cent), from natural cases of diarrhea, while the positive rates were only 14 per cent (3/22) and 22 per cent (5/22) for immunofluorescent staining of primary cultures of calf kidney cells infected with the specimens, and immune electron microscopy respectively. The advantages of the RPHA method are its simplicity, high sensitivity and rapidity.

Animals↗
PubMed1982

[Effect of infusion therapy on intrauterine fetal growth retardation (author's transl)].

It is requested for the control to the intrauterine fetal growth retardation (IUGR) to clarify the cause-and-effect relationship and to take the proper treatment under such a precondition. Following report covers the clinical report on the maternal infusion therapy mainly consisting of amino acids. 1) In the cases, to which the diagnosis of IUGR was given on basis of the development of symphisis-fundus length on the pregnogram and the fetal biparietal diameter (BPD), the occurrence of appropriate-for-gestational-age (AGA) is as extremely high as 44.5% when it is felt without treatment. In the abnormal patterns of symphisis-fundus length, consisting mainly of the threatened premature labor, the differential diagnosis is needed to the outward IUGR. 2) As the effect of the maternal infusion therapy mainly of amino acids on IUGR, given in addition to the oral nutrition, the increase of placental weight and fetal weight was observed at around NP-Cal/N of 99-116. No abnormality was observed in the maternal renal and liver function. 3) As the result of the maternal infusion therapy to 131 cases of IUGR, the occurrence ratio of the small-for-gestational-age (SGA) was 26.7%. No case of perinatal death or congenital malformation was observed.

Adult↗
PubMed1981

Role of mediodorsal thalamic nucleus in olfactory discrimination learning in rats.

Severe deficits in the acquisition of an olfactory learning-set task resulted from lesions of the central (olfactory) component of the mediodorsal thalamic nucleus but not from large lesions that destroyed olfactory projections to the amygdala. Complex olfactory learning may be mediated by the olfactory thalamocortical system and not by olfactory projections to the limbic system.

Amygdala↗
PubMed1981

Control of the variability of the afferent discharge rate in frog muscle spindle by potassium blockers.

Blockage of the K+ channels in the sensory terminal of frog muscle spindle by the combined application of BaCl2 and other K+ blockers makes it possible to reversibly change the sustained and irregular discharges into non-sustained regular discharges. As a similar change in the discharge pattern is also made by application of CoCl2 alone, though it is irreversible, the irregular and sustained discharge may be due to [Ca2+]i-activated potassium conductance.

Afferent Pathways↗
PubMed1980

Site of origin of calcium spike in frog muscle spindle.

In order to define the site of origin of the calcium spike along the sensory nerve terminal of the frog muscle spindle, changes in the amplitude of the spikes during long-lasting polarizations of the nerve terminal were compared with those of full-sized and abortive spikes which occurred spontaneously. The amplitude of the calcium spikes superimposed on a spindle potential was not modifiable by application of polarizing currents of 6 nA or less, as well as that of the abortive spikes; while the amplitude of the full-sized spikes depended upon the polarizations. These results suggest that the calcium spikes may initiate along the non-myelinated filaments, as in the abortive spike, in contrast with the full-sized spikes to generate at the terminal node of the myelinated axon.

Afferent Pathways↗