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Biomedical subjects

N Inoue

Publications and source records attributed to N Inoue.

At least 613 records · Page 34Linked to original sources
PubMed1983

B-cell differentiation antigen expressed on a human myeloma cell line (KMM-1).

KMM-1 is a human multiple myeloma cell line previously shown to express plasma cell phenotypes. Hybridomas against this cell line were produced by fusing spleen cells from hyperimmunized BALB/c mice with X63-Ag8-653 mouse myeloma cells. One monoclonal antibody derived from this fusion, termed 33-3, reacted with all the B-cell stages except the pre-B-cell stage. Both myeloma cells from patients and those from KMM-1 cells were reactive at the same maximum dilution of this antibody. No reactivity was seen with cells of T-lymphocyte origin, platelets, red blood cells, monocytes or granulocytes. The 33-3 antibody was unable to detect any specific marker distinguishing cultured myeloma cells from those that could not be cultured in vitro.

Animals↗
PubMed1983

[Ultrastructure of human oligodendroglioma].

Ten cases of human oligodendroglioma were examined with electron microscope. The materials were specimens derived through surgical operations. Results were as follow. The shape of tumor cells are various, round, oval, polygonal and irregular. The majority of tumor cells have round or ovoid nuclei, some have irregular nuclei or nuclear indentation. Chromatin distributions tend to clump. In the cytoplasm, there are commonly ribosomes, rough surfaced endoplasmic reticulums, mitochondria, microtubules and lysosome. Glial filament is rare or almost absent. Crystalline structures are seen in 3 cases. Cellular junctions, though immature, are seen in 4 cases. Perinuclear halo in the light microscopy was explained to be a fixation artifact in 10% formol. The samples, which were fixed in 2.5% cold glutaraldehyde and post-fixed in 1% osmium tetraoxide, did not show perinclear halo under the light and electron microscope. The specimen, which were fixed in 10% formol, were examined with electron microscope. Plasma membrane of the tumor cell was partly destroyed, intracytoplasmic organelles and matrix were also broken, and the cytoplasm was empty. It is confirmed in this study that empty cytoplasm represents the peculiar perinuclear halo in the light microscopy.

Brain Neoplasms↗
PubMed1982

Nerve growth factor induces specific enkephalin binding sites in a nerve cell line.

Specific enkephalin binding was found in a rat pheochromocytoma cell line, PC12 (subclone h), when cultured in the presence of nerve growth factor (NGF; 50 ng/ml). Specific binding of [3H][D-Ala2]Met-enkephalinamide was saturable with a KD=2.4 nM and Bmax = 866 fmol/mg of protein. The binding was displaced completely by morphine and naloxone at a concentration of 10(-4) and 10(-6) M, respectively. beta-Endorphin and dynorphin [1-13] also displaced the binding; KI = 9.6 and 81.6 nM, respectively. Enkephalin binding in PC12h cells cultured in the absence of NGF was very low. Binding was dramatically increased when cultured in the presence of NGF for 7 to 10 days. The enkephalin binding was induced by NGF in the cells cultured in a hormone-supplemented serum-free medium. In contrast, epidermal growth factor was of two orders lower potency, and insulin had no effect on the expression of enkephalin binding sites. Since the differentiation effects of NGF on PC12h cells are though to offer a model system to investigate the effect of NGH on neurons, the present observations may suggest a physiological role for NGF in the induction and maintenance of specific opiate receptors during development of the autonomic nervous system.

Animals↗
PubMed1982

Establishment and characterization of a human myeloma cell line (KMM-1).

A new cell line (KMM-1) was established from a subcutaneous plasmacytoma of a 62-year-old male with multiple myeloma. Immunological studies indicated that cultured cells were derived from the same clone of myeloma cells in vivo: smeared cells were stained with fluorescein-conjugated globulin of antisera monospecific to lambda-chain, and lambda-chains in the cell extracts and in culture media were identical to the Bence-Jones protein found in the patient's urine. The cell line grew in suspension with prominent nucleoli and rough endoplasmic reticulum. Cells had the karyotype of 47, X, -Y, Iq+, -2, +t(1:2) (cen:cen), +7, 12q+, 14q+, +mar and carried no Epstein-Barr virus-determined nuclear antigen. Surface markers were as follows; E rosette (-), IgG Fc receptor (-), C3 receptor (-), S-Ig (+), TdT (-), asialo-GM1 (-). The reasons for the successful establishment of the myeloma cell line are discussed.

Bence Jones Protein↗
PubMed1982

A polyhydric phenol sulfokinase and chronic liver injury.

The sulfokinase, that transfers activated sulfate from PAPS (3'-phosphoadenosine-5'-phosphosulfate) to nitrocatechol sulfate was studied. 1. It is thought that the enzyme plays an important role in detoxication by the sulfation of polyhydric phenols in liver. 2. The enzyme had an optimum at pH 8.0 in Tris-acetate buffer. Km was 0.105 x 10(-3)M. The rate of conjugation was linear within 1 min. 3. The main distribution of the sulfokinase was found in the liver, lungs, spleen and various other organs in the rat. The activity of the enzyme in rat liver was the highest in soluble fraction. In chronic hepatic injury caused by carbon tetrachloride injection, the activity of the enzyme gradually decreased to below about one half of the control value.

Animals↗