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N I Matvienko

Publications and source records attributed to N I Matvienko.

At least 37 records · Page 2Linked to original sources
PubMed1998

Site-specific endonuclease SscL1 I from strain Staphylococcus species L1.

A site-specific endonuclease SscL1 I preparation has been isolated and purified to near homogeneity from the strain Staphylococcus sp. L1 without admixtures of other nuclease activity. DNA cleavage proceeds according to the scheme: 5'-G down arrow ANTC-3' 3'-CTNA up arrow G-5', and thus the isolated enzyme is an isoschizomer of restriction endonuclease HinfI and belongs to the second class of restriction endonucleases. SscL1 I works over a broad range of temperature and pH. The enzyme is characterized by high stability during storage.

DNA, Viral↗
PubMed1998

Thermophilic strain Bacillus species AA contains several site-specific endonucleases.

Thermophilic strain Bacillus species AA contains several site-specific endonucleases and three of them have been identified. BspAAI recognizes the sequence 5'-C downward arrow TCGAG-3' and cleaves it after the first "C" forming 4-nucleotide 5'-ends and is an isoschizomer of XhoI. BspAAII recognizes the sequence 5'-T downward arrow CTAGA-3' and cleaves it after the first "T" forming 4-nucleotide 5'-ends and is an isoschizomer of XbaI. BspAAIII recognizes the sequence 5'-GGATCC-3' and is an isomer of BamHI. The optimal temperature and pH values are 42-48 degreesC and 7.0-8.0, respectively.

Bacillus↗
PubMed1997

Site-specific endonuclease AbaI from Azospirillum brasilense UQ 1796 is an isoschizomer of endonuclease BclI.

The site-specific endonuclease AbaI was isolated and purified to functional purity from the soil nitrogen-fixing bacterium Azospirillum brasilense UQ 1796. Purification included successive chromatography on columns with phosphocellulose, heparin-Sepharose, and hydroxyapatite. The purified enzyme recognizes the palindromic DNA sequence 5'-T decreases ATCA-3' and cleaves it as shown by the arrow. The isolated enzyme belongs to class II restriction endonuclease and is an isoschizomer of endonuclease BclI. The enzyme of AbaI is active at 26-56 degrees C. The optimal temperature is 48 degrees C and the optimal buffer is LRB.

Azospirillum brasilense↗
PubMed1997

Three site-specific endonucleases from thermophilic strain Bacillus species LA are isoschizomers of HhaI, AsuII, and HindIII.

Screening of thermophilic bacterial strains revealed a strain containing three site-specific endonucleases: BspLAI, BspLAII, and BspLAIII. These endonucleases were purified to functional purity by sequential chromatography. Recognition sites, DNA cleavage sites, and some properties of the endonucleases were determined. BspLAI recognizes the sequence 5-GCG/C-3 on the DNA molecule and is an isoschizomer of endonuclease HhaI. BspLAII recognizes the sequence 5-TT/CGAA-3 and is an isoschizomer of AsuII. BspLAIII recognizes site 5-A/AGCTT-3 and is an isoschizomer of endonuclease HindIII. All the three enzymes exhibit maximal activity at 55 degrees C. The optimal buffer is MRB, pH 7.4. They retain activity on storage for 3 weeks at room temperature and thus are highly stable.

Bacillus↗
PubMed1997

Site-specific endonucleases RspLKI and RspLKII from Rhodococcus species LK2 are isoschizomers of SphI and BamHI.

Two site-specific endonucleases, RspLKI and RspLKII, have been isolated and purified to functional homogeneity from the soil bacterium Rhodococcus species LK2. RspLKI recognizes the 5'-GCATG decreases C-3' DNA sequence and RspLKII recognizes the 5'-G decreases GATCC-3' sequence (arrows indicate DNA cleavage sites). The isolated enzymes are class II site specific endonucleases and are isoschizomers of endonucleases SphI and BamHI, respectively.

Chromatography, Ion Exchange↗
PubMed1997

Site-specific endonuclease from thermophilic Bacillus species MK strain is isoschizomer of SalI.

Screening of thermophilic bacterial strains revealed a strain containing site-specific endonuclease BspMKI. This endonuclease was purified to functional homogeneity during sequential chromatographic steps. The enzyme recognizes sequence 5'-G decreases TCGAC-3' on DNA molecule and is isoschizomer of endonuclease SalI. The molecular mass of BspMKI is about 45 kD. The enzyme is maximally active at 55 degrees C and MRB (50 mM NaCl, 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 1 mM dithiothreitol) is the optimal buffer. The enzyme is highly stable and retains its activity during two weeks at room temperature.

Bacillus↗
PubMed1997

Peculiarities of gene expression of the EcoRII modification-restriction system.

The restriction-modification genes of the EcoRII system have been cloned into plasmids under control of phage-specific promoters T7 and SP6. The transcription was induced by cell infection with the recombinant M13 phages with the corresponding genes of phage RNA-polymerases under control of the Plac-promoter in the presence of IPTG. The induction yields significant amounts of EcoRII DNA-methylase for both phage-specific promoters. In both cases no increase in EcoRII endonuclease expression could be achieved. We hypothesize that the expression of the endonuclease gene is regulated on the translational level.

Bacteriophage M13↗
PubMed1996

[Novel site-specific endonuclease from Acinetobacter species M strain].

Site-specific endonuclease R. AspMI was isolated and purified to apparent functional homogeneity from Acinetobacter species (strain M). The enzyme recognizes symmetrical DNA sequence 5'-AGG decreases CCT-3' and cleaves it at the site indicated by the arrow forming blind DNA ends. The endonuclease is an isoschizomer of the StuI endonuclease. Cleavage of the DNA site was inhibited by dcm-methylation. AspMI is approximately equal to 30 kD monomer.

Acinetobacter↗
PubMed1996

[Two site-specific endonucleases from the thermophilic OV Bacillus species strain].

Two site-specific endonucleases, BspOVI and BspOVII, were isolated from a thermophilic strain Bacillus species OV. The activities of both enzymes are maximal at 48 degrees C and do not depend on ATP and S-adenosyl-L-methionine. BspOVI recognizes the sequence [sequence: see text] and cleaves it as indicated by arrows. Thus, BspOVI is a IIN-subclass endonuclease isoshizomer of Eam1105I. BspOVI is very stable during storage. The enzyme can be used for direct T/A cloning of PCR products. BspOVII recognizes and cleaves the sequence [sequence: see text]; thus, BspOVII is an isoshizomer of CIaI. The cleavage by BspOVII is blocked by dam methylation of adenine inside the recognition site.

Bacillus↗
PubMed1996

[Site-specific endonuclease BspR7I from thermophilic strain of Bacillus sp. R7].

A site-specific endonuclease which recognizes the sequence 5'-CCTNAGG-3' was purified to homogeneity from the thermophilic strain Bacillus sp. R7. The endonuclease (BspR7I) is monomeric protein with an apparent molecular weight of 37 kD. The enzyme is active over a wide range of NaCl concentrations, pH, and temperatures. BspR7I cleaves DNA substrates according to the scheme: 5'-CC decreases TNAGG-3' 3'-GGANT increases CC-5', hence the endonuclease represents an isoschizomer of Bsu361.

Bacillus↗
PubMed1995

[Site-specific endonuclease BspKT8 from the thermophilic strain KT8 of Bacillus species].

A site-specific endonuclease capable of recognizing the sequence 5'-AAGCTT-3' was detected and purified to homogeneity from the thermophilic strain of Bacillus species KT8. The endonuclease has a molecular mass of 34 kDa and is found in solution in a monomeric form. The activity of BspKT8 does not depend on ATP and is not stimulated by S-adenosyl-L-methionine. The enzyme displays the highest activity with a broad range of temperatures (37 degrees-48 degrees C). Since DNA cleavage occurs in accordance with the scheme: [formula: see text] the enzyme can be assigned to the class-II of restriction endonucleases and represents an isoschizomer of HindIII.

Bacillus↗
PubMed1995

[A method of selective PCR-amplification of genomic DNA fragments (SAGF method)].

A method for separating into definite sets of a complex mixture of fragments obtained by DNA cleavage with IIS- or IIN-types of restriction endonucleases producing single-stranded termini of different sequences at the fragment ends has been developed. The method is based on the ligation of short double-stranded adapters with single-stranded termini complementary to the termini of a selected set of fragments followed by PCR-amplification with the primer which represents a strand of the adapters. Using endonucleases BcoKI and Bli7361 recognizing sequences CTCTTC and GGTCTC and producing three- and four-nucleotide 5'-termini, respectively, it has been shown that amplification of a set of fragments occurs only when the adapters are attached to DNA fragments with DNA-ligase. Several applications of the SAGF-method are suggested: for obtaining individual bands in DNA fingerprinting; for reducing the kinetic complexity of DNA in the representational difference analysis (RDA method) of complex genomes; for cataloguing DNA fragments, and for constructing physical genomic maps.

Animals↗
PubMed1995

[Site-specific endonuclease and methylase from thermophilic bacteria of Bacillus species IS4].

The site-specific endonuclease R . BspIS4I and methylase M . BspIS4I have been isolated and purified to functional purity from the thermophilic strain of Bacillus species IS4. R . BspIS4I recognizes sequence [sequence: see text] on the DNA and cleaves it as indicated by the arrows to form single-stranded 4-nucleotide 5'-protruding termini. The enzyme is an isoschizomer of BbvII. M . BspIS4I is related to adenine-specific methylase.

Bacillus↗
PubMed1995

[A new site-specific endonuclease from Bacillus species ST5].

A site-specific endonuclease R.BspST5I has been isolated in a functionally pure state from the thermophilic strain of Bacillus species ST5. The enzyme recognizes sequence 5'-GCATC-3' on the DNA and splits it at a distance of five nucleotides from the 3'-end of the recognition site as well as at distances of nine or ten nucleotides at the complementary filament depending on the hydrolyzed sequence of the DNA. The enzyme is a isomer of endonuclease SfaNI from Streptococcus faecalis ND547.

Bacillus↗