Search PubMed⌕ Search

Biomedical subjects

N I Drize

Publications and source records attributed to N I Drize.

At least 55 records · Page 3Linked to original sources
PubMed1989

[Identification of primitive hematopoietic stem cells in mice by the limiting dilution method].

An absolute number of the primitive stem cells (PSC) providing for prolonged maintenance of hemopoiesis in culture was measured with the aid of the limiting dilution method. The number of the PSC was determined according to the magnitude of the "zero" class cultures, i. e. of the cultures in which CFUs could not be demonstrated after 5-8 weeks. The fitness of the method can be proved by the presence of the linear relations between the number of the explanted stem cells and the number of the PSC as well as by the passage of the regression curve through the origin of coordinates. The data on the content of the PSC functioning in vivo and in vitro turned out similar, amounting to about 100 cells per mouse femur.

Animals↗
PubMed1989

[Effect of endothelial and smooth muscle cells and the extracellular matrix produced by them on hematopoiesis].

The influence of endothelial and smooth muscle cells and their extracellular matrices on hemopoiesis in long-term bone marrow cultures was investigated. In contract to smooth muscle cells, the enrichment of adherent cell layer with endothelial cells led to the stimulation of hemopoietic cell and granulocyte-macrophage progenitor (CFU-GM) production. The extracellular matrices of both smooth muscle and endothelial cell origin also produced a stimulating effect on the culture, although they were not so effective as endothelial monolayers. The cultures from cryopreserved bone marrow could be established only with the help of endothelial cells or any types of extracellular matrices.

Cells, Cultured↗
PubMed1989

[Long-term culture of bone marrow from patients with PH'-positive chronic myeloid leukemia].

Long-term cultures from bone marrow of patients with chronic myeloid leukemia were obtained. The cultures were characterized by the reduced period of hemopoiesis. The enrichment of the adherent cell layer with endothelial cells or their extracellular matrix was not stimulative. The Ph'(+) malignant cells persisted in culture during the whole period of hemopoiesis. The data obtained indicate that the long-term bone marrow culture system is not suitable for the elimination of malignant cells from leukemic bone marrow.

Bone Marrow↗
PubMed1988

[The proliferative potential of hematopoietic stem cells (CFUs) during serial passage in irradiated mice as foci of ectopic hematopoiesis].

During serial passages of hemopoietic tissue as a whole ectopic hemopoietic site under renal capsule of irradiated mice gradual decrease of proliferative potential of CFUs has been observed. The results suggest that the most probable cause of such decline is random loss of precursor cells with high but limited proliferative potential during serial passages of hemopoietic tissue.

Animals↗
PubMed1985

[Renewal of hematopoietic stem cell clones in long-term bone marrow cultures].

In long-term cultures of murine bone marrow, clonal succession of hemopoietic cells was observed as measured by karyologic analysis. There were high oscillations in self-renewal of CFUs in the cultures. A close correlation between the CFUmix karyotype and mitotic non-adherent cells in culture (but not between these cell types and CFUs) was revealed.

Animals↗
PubMed1984

[Hybrid resistance in a long-term bone marrow culture and in foci of ectopic hematopoiesis formed in the culture].

The lack of hybrid resistance to the bone marrow graft has been demonstrated in a long-term bone marrow culture. After adherent cell layer transplantation into the body the hybrid resistance was demonstrated in de novo formed ectopic hemopoietic foci. The resistance was of the recipient type, because of which the existence of the migrating component of the hemopoietic microenvironment is suggested.

Animals↗
PubMed1984

[Incorporation of yeast tRNA into mouse L 1210 lympholeukemic cells].

[32P]tRNA from baker's yeast is incorporated without degradation into lympholeukotic cells of L1210 mice. The tRNA incorporation determined after tRNA hydrolysis on cell surface by RNAase increases linearly with a rise in the initial concentration from 0.5 to 500 micrograms per ml. According to gel electrophoresis of intracellular nucleic acids, after a 3 hour incubation the [32P]tRNA incorporated into the cells by 50% to form tRNA fragments without any conspicuous reutilization. The kinetic curve of tRNA incorporation during the first 60 min demonstrates a severalfold decrease in the initial maximal incorporation of [32P]tRNA into the cells (2 min), with a subsequent restoration of the incorporation within 2-3 hours.

Adsorption↗
PubMed1984

[Progenitors of transitory spleen colonies in mouse embryonal liver].

The transitory nature of about half of spleen colonies macroscopically detectable in the spleen 7 days after injection of embryonal liver hemopoietic cells was demonstrated by localization of the colonies on the spleen surface and by the study of the content of polypotential and unipotential precursors in individual 7- and 11-day colonies produced in the spleen of irradiated mice by the cells of early (12-13-day) and late (17-18-day) embryonal liver.

Animals↗
PubMed1983

[Blast cell differentiation of a continuous human leukemia line as affected by dimethyl sulfoxide].

The human blast cells line L-101 isolated from blood leukocytes of the patient with the cutaneous erythromyeloleukemia in the media with dimethyl-sulfoxide (DMSO) and maintained by permanent cultivation stimulate myeloid differentiation of this cells. The number of differentiated cells depends on DMSO concentration. Maximum of differentiated cells (up to 75%) have exposed on the 6th day of incubation with 0,75% DMSO. The cell line L-101 is a suitable model for investigation of myeloid differentiation.

Cell Line↗