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Biomedical subjects

N I Drize

Publications and source records attributed to N I Drize.

At least 37 records · Page 2Linked to original sources
PubMed1997

[Immunoglobulin G monoclonal human anti-rhesus Rho(D) to prevent rhesus-incompatibility].

RhD immunisation which follows pregnancy can be prevented by administration to the rhesus-negative mother of 20 micrograms anti-D immunoglobulin per 1 ml of D-positive fetal red cells in the maternal circulation. With the aim of substitution of polyclonal anti-D with monoclonal one we developed human-mouse cell lines producing anti-RhD IgG1 by fusing EBV-transformed human immune lymphocytes with murine myeloma. After several clonings and passages the EBV genome was eliminated from the cell lines. The antibodies were purified from culture supernatants using protein A affinity chromatography and tested for sterility, virus contamination, pyrogenecity, toxicity and DNA content. The monoclonals were compared with the standard polyclonal anti-RhD in in vitro and in vivo assays. In antibody-dependent cell cytotoxicity (ADCC) 2 of 4 studied monoclonals promoted greater RBS lysis than polyclonal anti-D at equivalent concentrations. Detection of binding site number of monoclonals anti-D revealed about 10,000/RBS D-determinants on DCe/dce erythrocyte which agrees with the data for polyclonal anti-D. Best in ADCC monoclonal anti-D sharply increased human autologous 51Cr-labelled rbc sensitised in vitro as well as accelerated clearance of RhD RBS from circulation of Rh-negative volunteers injected with 150 micrograms monoclonal anti-D. After clinical trial using of monoclonal anti-D is permitted in Russia.

Blood Group Incompatibility↗
PubMed1993

[The introduction of the marker gene Neor into hematopoietic stem cells by electroporation].

An electroporation method has been used to introduce marker gene Neor into mouse stem hemopoietic cells which are capable of long-term hemopoiesis maintenance in marrow long-term cultures. Integration of the gene was tested by polymerase chain reaction. The effect of the procedure averaged 50-80% of marked CFUc. Electroporation did little damage to hemopoietic cell precursors. Gene transfer can be made most effectively using bone marrow from mice injected 5-fluorouracil 4 days prior to the experiment.

Animals↗
PubMed1991

[Human B-lymphoblastoid cell lines: optimal conditions for their procurement, selection, cultivation and cloning].

The protocol of Epstein-Barr virus (EBV)-induced transformation of B-lymphocytes from reripheral blood of normal humans has been described. The requirements for mononuclear EBV-infection and for selection, cultivation, and cloning of EBV-transformed cell lines have been investigated. Successful establishment of stable human immunoglobulin-producing cell lines from lymphocytes of immunized donors could be achieved following the protocol presented.

B-Lymphocytes↗
PubMed1991

[Transduction of oncogene RAS into antibody-producing hybridoma].

The effect of introduction by means of electroporation of protogene ras into cells of antibody-producing hybridoma was studied. Doubling time (TD) of transduced subclones, in which H-ras gene was expressed, proved to be 1.8-fold shorter as compared to the parent line. Other characteristics of the hybridoma (cloning efficiency, antibody production in vitro, the time of ascitic fluid appearance in mice and antibody titer in vivo) were not changed. Addition of 12-0-tetradecanoylphorbol acetate to the culture did not alter the growth parameters of H-ras transduced subclones.

Animals↗
PubMed1991

[Effects of polysaccharide from Thuja occidentale L. on stromal precursor cells of hematopoietic microenvironment in mice].

The effect of high molecular polysaccharide subfraction from Thuja occidentale L. (TPS) on stromal precursor cells of hematopoietic microenvironment under the "steady-state" conditions and after sublethal irradiation was investigated. The stromal precursor cells of different stages of differentiation were detected by the implantation of mouse bone marrow under the renal capsule of syngeneic intact recipients and chimeras. It was shown that TPS did not occur the toxic influence on the stromal precursor cells and provided the defense effect on them under the strong (6 Gy) radiation damages.

Animals↗
PubMed1991

[Transduction of a marker gene (Neor) into precursor cells of the hematopoietic microenvironment].

The attempt of retroviral transfer of the bacterial Neor gene into stromal precursor cells able to transfer haemopoietic microenvironment and to long-term support of haemopoiesis in vitro and in vivo was made. The existence of marker gene in stromal cells was established by the method of polymerase chain reaction. The transduced stromal precursor cells create normal haemopoietic microenvironment. The data obtained would be important for the further investigation of proliferation and differentiation of stromal precursor cells.

Animals↗
PubMed1990

[Primary hemopoietic stem cell].

A complex of experimental original laboratory findings is presented to demonstrate a restricted proliferative potential of stem hemopoietic cells. Development of new methodologies to study primitive stem hemopoietic cells, i. e. the cells that are responsible for maintenance of continuous blood formation, allowed for confirming their existence, assessing their content in the hemopoietic tissue, determining the time and site of their embryonic origin, and for showing their limited capacity for regeneration. The obtained evidence is important for the understanding the mechanisms governing hemopoiesis.

Animals↗
PubMed1990

[Early hematopoietic stem cells (pre-CFU) studied in long-term culture of murine bone marrow].

A new category of primitive hemopoietic stem cells determined in murine bone marrow long-term culture (CFU-BMLTC) is described. The novel precursors give rise to hemopoietic colonies corresponding to "cobble stone" areas observed on the adherent cellular layer. The linear relationship between the colony number and the dose of bone marrow cells explanted in the culture has been discovered. Radiosensitivity of the new type of hemopoietic precursors in the bone marrow of adult mice is characterized by D0-1.28 Gy. CFU-BMLTC were also identified in 13-day mouse fetal liver. In contrast to CFUs the precursors described are less sensitive to extensive in vivo treatment with hydroxyurea. The data obtained have permitted the authors to make a conclusion on a higher status of CFU-BMLTC as compared to CFUs in the hemopoietic stem cell hierarchy.

Animals↗