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Biomedical subjects

N Fujimoto

Publications and source records attributed to N Fujimoto.

At least 289 records · Page 16Linked to original sources
PubMed1985

Inhibition by 2-bromo-alpha-ergocriptine and tamoxifen of the growth of an estrogen-dependent transplantable pituitary tumor (MtT/F84) in F344 rats.

A new transplantable pituitary tumor, designated MtT/F84, was induced in estrogenized female F344 rats and has been serially passaged in 17 beta-estradiol-treated females. It grew well in rats treated with estrone, 17 beta-estradiol, or estriol but not in intact females or in rats given progesterone or testosterone. The growth of MtT/F84 in rats grafted with up to 1.6 X 10(6) tumor cells and given 17 beta-estradiol was inhibited by orally administered high dose bromocriptine (37.5 mg/kg in food) or by intraperitoneal injection of tamoxifen citrate but was not inhibited by low dose bromocriptine (3.75 mg/kg in food). The tumors grown in intact females contain high amounts of estrogen receptor, and they were greatly reduced in the tumors grown either in 17 beta-estradiol or 17 beta-estradiol-plus-tamoxifen loaded rats. However, administration of bromocriptine resulted in estrogen receptor levels significantly higher than those of tumors grown in 17 beta-estradiol. The existence of dopamine receptor was also confirmed. Growth inhibition of MtT/F84 either by high dose bromocriptine or by tamoxifen may be a direct action and may be an estrogen and dopamine receptor-mediated phenomenon.

Animals↗
PubMed1984

Separation of heparin on Sepharose CL-4B in the presence of high concentrations of ammonium sulphate.

Heparin was fractionated by chromatography on Sepharose CL-4B/3.8-2.0 M ammonium sulphate in 0.01 M hydrochloric acid at 4 degrees C based on a principle different from that of gel filtration--possibly due to multiple interaction mechanisms including those based on hydrophobic bonds. The results of the fractionation were very similar to those of chromatography on Phenyl-Sepharose CL-4B/3.8-2.0 M ammonium sulphate in 0.01 M hydrochloric acid at room temperature. That is, the separation was related to the molecular size distribution, N-acetyl content and anticoagulant activities. As the result of studies on a set of Sepharose 4B gels with different degrees of cross-linking, it has been shown that the introduction of the cross-linked structure, -O-CH2-CH(OH)-CH2-O-, into the gel matrix enhances the interaction between the Sepharose CL-4B gel and heparin, indicating that heparin retention by the highly cross-linked Sepharose 4B gel surpasses that by Phenyl-Sepharose CL-4B.

Ammonium Sulfate↗
PubMed1984

The studies on the role of gastric glycoproteins with reference to cytoprotection: protective effect of prostaglandin E2 and sofalcone on ethanol-induced necrosis.

The gastric cytoprotective action of prostaglandin E2 (PGE2) and sofalcone was studied in rats. The in vitro incorporating activity of 3H-glucosamine into the gastric macromolecular glycoproteins was examined when PGE2 (0.1 mg/kg, p.o.) or sofalcone (100 mg/kg, i.p.) was administered 5, 15 or 30 min before the oral administration of absolute ethanol. The cytoprotective effect of PGE2 against gastric mucosal damage was demonstrated 5 min after PGE2 was given orally. The cytoprotective effect by sofalcone was seen after 15 min. However, during this period, the decrease in gastric macromolecular glycoprotein synthesis induced by the ethanol damage could not be restored by pretreatment with PGE2 or sofalcone. On the other hand, the reduction in the content of the gastric macromolecular glycoproteins by the ethanol damage was found to be prevented to a significant extent by pretreatment with PGE2. The same phenomenon was also observed in the administration of sofalcone. Accordingly, PGE2 has stimulating effect on the gastric glycoproteins biosynthesis, but this effect can not be considered as the mechanism responsible for cytoprotection, if indeed a single mechanism exists. Thus, it is suggested that the adhesion or maintenance of secreted macromolecular glycoproteins to the gastric tissue is closely related to cytoprotection.

Animals↗
PubMed1983

[Phase II study with methyl-6[[[2-chloroethyl) nitrosoamino] carbonyl] amino]-6-deoxy-alpha-D-glucopyranoside (MCNU) in hematological malignancies].

A total of 117 cases with hematological malignancies were treated with MCNU at doses of 70-100 mg/m2. Following are the results obtained. 1. MCNU showed a marked depression of cells in the cases with CML, polycythemia vera and thrombocythemia. The low level of cells was maintained for 2 to 7 months. 2. A good response was observed in several cases with blastic crises of CML. 3. No response was observed in two cases with acute leukemia. 4. Although a fair response was observed in several cases with malignant lymphoma or multiple myeloma, moderate bone marrow suppression was observed in a majority of the cases.

Adult↗
PubMed1983

B-cell malignancies and differentiation antigens defined with monoclonal antibodies.

Monoclonal antibodies directed to B-cell lineage were produced. They were characterized by their reactivity with a variety of human hematopoietic cell lines and normal lymphoid cells and by the immunohistological distribution of cells positive for the antibodies. Some of them were specific for B-cell lineage at various stages of B-cell differentiation and some were, in addition, cross-reactive with other cell lineages. These newly produced antibodies were utilized to dissect and characterize B-cell malignancies and were applied for classification of B-cell malignancies.

Animals↗
PubMed1983

[Clinical effect of cefroxadine on surgical infections].

Cefroxadine (CXD), an orally active cephalosporin antibiotic, has a broad spectrum and a bactericidal action. The efficacy of CXD in the surgical field was investigated and the following results were obtained. CXD was administered to 31 cases in all; 13 cases with mastitis, 9 with wound infection, 4 with infected atheroma, 3 with periproctal abscess and 2 with phlegmon, respectively. The daily dose was ranged from 750 mg to 1,500 mg. Clinical effects were good in 27 cases and fair in 4 cases, and the effective rate was 87.1%. As to side effects, a slight diarrhea was observed in 1 case, but the symptom was disappeared after 2 days without a special treatment.

Administration, Oral↗
PubMed1982

Catecholamine level in cerebrospinal fluid of epileptics.

We estimated catecholamine levels in CSF of 15 epileptics and 75 non-neurological patients utilizing a high performance liquid chromatograph with a highly sensitive fluorometer and found the following results: The dopamine (DA) levels in males were significantly higher than those in females, while norepinephrine (NE) levels in males were the same as in females. The DA levels were significantly lower and NE levels significantly higher in epileptics than in non-neurological patients. DA and NE in petit mal patients were on the average lower than in grand mal patients, but untreated grand mal patients had higher NE levels. These results suggest that epilepsy may be associated with a disturbance of DA and/or NE metabolism or release in the brain.

Adolescent↗
PubMed1982

Fluorometrical analysis of guanidino compounds in human cerebrospinal fluid.

Guanidino compounds in CSF of 57 human subjects were determined fluorometrically after reaction with phenanthrenequinone in alkali solution, using HPLC. Creatinine (65.2 +/- 13.4 nmol/ml), arginine (24.7 +/- 6.4 nmol/ml), and homoarginine (0.7 +/- 0.3 nmol/ml) were found in all subjects. Trace amounts of guanidinosuccinic acid and guanidinoacetic acid were detected in some of the subjects. Brain guanidino compounds, taurocyamine, N-acetylarginine, and methylguanidine were not detected in CSF.

Adolescent↗
PubMed1982

[Antibodies against human lymphocytes].

The introduction of hybridoma technology has greatly contributed to the identification and the characterization of a variety of cell surface antigens present on human lymphoid cells. Rapid progress has recently been made in generating monoclonal antibodies against human lymphoid cells and extensive studies in clinical medicine have defined the potential application of these monoclonal antibodies. In this review, we summarize the main similarities and differences among these monoclonal antibodies.

Animals↗