Search PubMed⌕ Search

Biomedical subjects

N Carter

Publications and source records attributed to N Carter.

At least 145 records · Page 8Linked to original sources
PubMed1979

Synthesis of carbonic anhydrase in rabbit and chicken reticulocyte lysates.

The synthesis of carbonic anhydrase, the second most abundant soluble protein of red blood cells, is shown to occur in rabbit and chicken reticulocyte lysates. This translation product was identified by chloroform/ethanol extraction, polyacrylamide gel electrophoresis in sodium dodecylsulphate and peptide mapping. In rabbit retic-locyte lysates, predominantly one of the two red cell isozymes, carbonic anhydrase I, is synthesised. The proportion of carbonic anhydrase synthesis (0.2-0.8% of total protein synthesis) in vitro is comparable to that (0.2-1.0%) in vivo for both rabbit and chicken reticulocytes.

Amino Acids↗
PubMed1979

Characterization of human carbonic anhydrase III from skeletal muscle.

A third form of human carbonic anhydrase (CA III), found at high concentrations in skeletal muscle, has been purified and characterized. This isozyme shows relatively poor hydratase and esterase activities compared to the red cell isozymes, CA I and CA II, but is similar to these isozymes in subunit structure (monomer) and molecular size (28,000). CA III is liable to posttranslational modification by thiol group interaction. Monomeric secondary isozymes, sensitive to beta-mercaptoethanol, are found in both crude and purified material and can be generated in vitro by the addition of thiol reagents. Active dimeric isozymes, generated apparently by the formation of intermolecular disulfide bridges, also occur but account for only a small proportion of the total protein and appear only when the concentration of CA III is particularly high.

Adult↗
PubMed1979

Glucose-6-phosphate dehydrogenase deficiency in a native Danish family. A new variant.

Deficiency of red cell glucose-6-phosphate dehydrogenase was found in a native Danish family, in which 2 boys suffered from severe haemolytic anaemia. The mother and 3 sisters of the boys were heterozygotes for G-6-PD deficiency. The biochemical investigations indicate that this deficient G-6-PD is very similar to the Mediterranean variant; however, this variant gene may represent another example of G-6-PD 'Helsinki' or an unique variant with properties similar to G-6-PD B(--).

Adult↗
PubMed1978

Medical rehabilitation unit--Palmerston North, the first four years.

The first four years of the Palmerston NORTH Medical Rehabilitation Unit are described. Reference is given to buildings, patients and staff considerations and organisation of the Unit. The outcome of the Unit's activities are discussed. Evaluation is attempted. Attention is drawn to the value of work assessment in a hospital setting.

Adolescent↗
PubMed1976

SI units in clinical pathology.

By now, most doctors in Australia will have had some experience with the new SI units which are being used for pathology test results, especially chemical pathology. While the usage of SI units has spread rapidly through public hospitals in Australia, spread in the non-hospital sector has been slower. However, the change will occur, and so the following is presented to assist general practitioners to understand the reasons for the change, and to interpret results expressed in the new system.

Blood Chemical Analysis↗
PubMed1975

Characterisation of carbonic anhydrases from tissues of the cat.

1. Red blood cells from several members of the cat family have been found to contain only carbonic anhydrase (EC 4.2.1.1) isozymes of the "high activity" (carbonic anhydrase II class) in red blood cells. 2. Two carbonic anhydrase II type isozymes have been isolated from red cells of the domestic cat. Kinetic and structural characterisation shows that the two isozymes have identical composition except for a probable difference of one amide group. The general characteristics of cat red cell carbonic anhydrase are similar to other mammalian carbonic anhydrase II isozymes, in particular the dog carbonic anhydrase II. 3. A carbonic anhydrase I (low activity) isozyme has been isolated and characterised from cat caecal mucosa. This carbonic anhydrase I is similar to the carbonic anhydrase I extracted from canine red cells. Comparative studies showed that whereas members of the dog family have carbonic anhydrase I and carbonic anhydrase II in red cells the cat family has only a carbonic anhydrase II type isozyme, however, cat liver and spleen homogenates were also found to contain carbonic anhydrase I identical to that found in caecum.

Amino Acids↗
PubMedDate not supplied

Interferon-gamma enhances mRNA and surface expression of class I antigen on human extravillous trophoblast.

Human trophoblast cells with immunocytochemical characteristics of the extravillous population have been isolated from 1st trimester placentae. Treatment of these cells with IFN-gamma increases the expression of Class I antigens at both the cell surface and mRNA level. A similar increase in Class I antigens is also found in JEG-3 choriocarcinoma cells after treatment with IFN-gamma. The possibility that aberrant production of IFN-gamma may upset the fetal-maternal equilibrium in vivo is discussed.

Antigens, Surface↗
PubMedDate not supplied

Polycystin expression during embryonic development of human kidney in adult tissues and ADPKD tissue.

Normal renal tissue, ranging from 8 weeks' gestation to full term to adult, was probed with polyclonal antibodies raised to peptide epitopes within the translated PKD1 gene sequence. Three antibodies were studied, all of which gave similar results. Renal tissue from patients with autosomal dominant polycystic kidney disease (ADPKD) and samples from normal adult liver, heart, brain, skeletal muscle and lymph node were also studied. Tissue staining demonstrated that the pattern of polycystin expression changed with gestational age in normal kidney. Whereas the precursors to the renal excretory unit were stained at 12 weeks, and the proximal and distal convoluted tubules stained to differing degrees throughout development, the glomeruli were poorly stained until full term and also in the adult. Extrarenal tissue stained in both adult and juvenile samples, with the exception of lymph node, which remained unstained. The intensity of polycystin staining increased in ADPKD renal tissue. The widespread distribution of polycystin was consistent with the systemic nature of ADPKD and the role of epithelial cells in the disease.

Adult↗
PubMedDate not supplied

Fatty change of the pediatric myocardium.

The myocardium was studied for the presence of fat in 980 pediatric deaths over a wide age range during a 10-year period. Of these, only 67 cases (7%) were found to have fatty change. The cause of death varied widely and no common pathogenetic factor was identified. Infections and congenital disorders constituted the most frequent causes of death associated with fatty change. The degree of fatty change was variable. Severe fatty change correlated with severe panacinar fatty change of the liver. The pathogenesis of the lesion is discussed.

Adipose Tissue↗
PubMedDate not supplied

Acute sternal opening after cardiac surgery in children: effect on plasma atrial natriuretic peptide.

Plasma atrial natriuretic peptide (ANP) was measured immediately before and 15 minutes after re-opening of the sternotomy and delayed sternal closure were performed to alleviate possible myocardial compression in 3 children. As acute re-opening of the sternotomy is a rare occurrence our sample size was necessarily small. ANP increased following the procedure in all 3 patients, and this was significant (p less than 0.02) when compared to plasma levels obtained from 9 children who underwent sternotomy prior to elective cardiac surgery. Following acute re-opening of the sternotomy there was an increase in median urine output and a decrease in median central venous pressure. Our results illustrate that it is atrial wall stress rather than intra-atrial pressure that determines release of ANP, and in addition suggest ANP may be a factor in the rapid clinical improvement following delayed sternal closure for atypical tamponade in children.

Atrial Natriuretic Factor↗