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N A Probatova

Publications and source records attributed to N A Probatova.

At least 19 recordsLinked to original sources
PubMed1992

[Epithelial membrane antigen in lymphosarcoma cells (immunomorphologic study)].

11 cases of different types of lymphosarcoma of T- and B-nature are studied immunohistochemically by means of antibodies against epithelial membrane antigen (EMA), including new Soviet monoclonal antibodies ICO-25 to this antigen, common leucocytic antigen, vimentin and cytokeratin. The phenomenon of the EMA including ICO-25 binding to the cells of some lymphosarcomas is confirmed. This indicates that the use of only this "epithelial differentiation marker" in not sufficient for the differentiation between lymphosarcoma and poorly differentiated carcinoma. Variability of lymphosarcoma cells staining by vimentin antibodies is shown, other limitations of the immunohistochemical examination of lymphoma are discussed. The conclusion is made of the necessity to use the spectrum of the above antibodies when differentiating between lymphosarcoma and poorly differentiated carcinoma.

Antigens, Differentiation↗
PubMed1991

[The treatment procedure in olfactory (esthesioneurogenic) tumors].

In 1968-1988, eleven cases of olfactory neurogenic tumors were seen at The All-Union Center for Oncology Research, Moscow which made up 9.5% of the total of nasal cavity cancers. Morphologically, esthesioneurocytoma was diagnosed in two patients whereas esthesioneuroblastoma--in nine. Four patients received combined therapy while seven--chemotherapy and radiation. Progression of the disease was registered in 7 out of 11 patients: in 3 of 4 cases receiving combination treatment and in 4 out of 7 those treated conservatively. Four cases died within 7-29 months while 7 remain alive: two patients at 18 and 156 months following combined therapy and 5--at 18, 18, 24, 84 and 132 months after chemoradiation treatment. Radiation alone proved the best therapeutic option. Surgery is justified in cases with residual tumor as second-line procedure.

Adolescent↗
PubMed1990

[The morphological diagnosis of malignant non-Hodgkin's lymphomas (lymphosarcomas)].

On the basis of morphoimmunological correlates certain criteria have been elaborated helpful in diagnosis of non-Hodgkin's malignant lymphomas (NML) of both B- and T-cell origin, and this was reflected in the modified Kiel's classification. The group of T-cell NML has considerably increased. T-cell origin of Lennert's lymphoma and angioimmunoblastic lymphadenopathy has been proven. Morphological substrate of NML from peripheral T-lymphocytes became more precise. The tumours, depending on the predominant cells, are subdivided into small cell (T-zones and pleomorphic lymphoma of small cells), mixed cell (pleomorphic lymphoma of medium-size and large cells) and large cell lymphoma (immunoblastic and large cell anaplastic Ki-I+). Criticism of T-NML systematization is due to the lack of definite cytological criteria because of the extreme morphological heterogeneity of peripheral T-lymphocytes and different interpretation of the clinical course of the established morphological variants. Among B-cell lymphomas the problem of the so-called intermediate lymphocytic lymphoma (ILL) and its variety--a mantle-zone lymphoma as well as monocytoid B-cell lymphoma is discussed in the literature. It is established in immunological testing that the cells of ILL possess a phenotype of cells of the primary follicles and those of the mantle-zone of the secondary follicles while the cells of the monocytoid B-cell NML have a peculiar unique phenotype similar to that of cells in the marginal zone of the spleen follicles.

Humans↗
PubMed1989

Two different anti-erythroid monoclonal antibodies in immunodiagnosis of human leukemias: a comparative study.

To date, only anti-glycophorin-A monoclonal antibodies (MAbs) have been widely used as anti-erythroid probes in the diagnosis of leukemias. We have examined blood, bone-marrow and lymph-node samples from 474 patients, adults and children, with different hemopoietic malignancies, using a panel of MAbs including 2 anti-erythroid MAbs directed to glycophorin-A and an antigen of erythroblasts, Ag-Eb. MAb HAE9 directed against a human epitope of Ag-Eb has earlier been shown to be highly specific for immature erythroid cells. Of all the patients, 2.7% demonstrated glycophorin-A expression on blast cells, while anti-Ag-Eb MAb HAE9 reacted positively with cells from 6.0% of patients. Samples from 31 of 474 (6.5%) patients expressed one or both erythroid markers. Our results indicate that MAb HAE9 may be useful, in combination with anti-glycophorin-A MAbs, as an anti-erythroid probe for immunophenotyping human leukemias.

Adult↗
PubMed1989

Chromosomal characteristics of malignant lymphoma.

Results of a cytogenetic and morphological study of 60 malignant lymphomas (ML) are presented. The most often observed chromosome abnormalities were rearrangements involving 14q32, 11q13, 11q21-23, 6q15, 6q21, 12p11-12, 17p11-12, and extra chromosomes 18, 3, 21 and 7. Translocations involving 14q32, leading to the appearance of marker 14q+, were noted in 41% of the tumors. Strict correlations between karyotype and morphology of ML were not seen. However, rearrangements of 11q were mostly found in low-grade tumors and markers 6q- in high-grade tumors. The absence of t(14;18), which is regarded to be the most common abnormality in ML, and an unusually high incidence of t(11;14) in our series confirm the uneven geographical distribution of ML with these translocations. Chromosome abnormalities in ML and acute lymphoblastic leukemia are compared.

Adolescent↗
PubMed1989

[The clinical characteristics of hematosarcoma with primary skin involvement].

As many as 90 patients suffering from hematosarcomas associated with primary skin lesions were examined. This patients' group did not include patients with mycosis fungoides or with Sezary's syndrome. The clinical picture, ways and stages of the spreading of skin hematosarcomas were reviewed. Damage to the internal organs outstripped lymph nodes injuries in 1/3 of the patients. Half of the patients followed up until their death died at the height of acute leukemia. The survival appeared relatively high: the median period turned out to be 51 months, whereas 33% of the patients survived for 10 and more years. The survival was influenced by the sex and age of the patients as well as by the histological disease variant.

Adolescent↗
PubMed1989

[Pathologic anatomy of subtypes of immunoblastic lymphosarcoma].

Morphological parameters for immunoblastic lymphosarcoma (IL) subtypes: plasmocytic IL (PIL) and polymorphocellular IL (PMIL) were determined through a comparative morphological analysis of 56 sections and 64 respective diagnostic biopsies. PIL was found to more frequently affect the gastrointestinal tract. Tumors were found to be selective in damaging the organs, e.g. white pulp was affected in PIL, marginal follicular areas, in PMIL. Clinical and anatomical analyses of the death causes in patients with IL showed that they all had died of neoplastic progression (67.9%), concurrent diseases (23.2%) or complications resulted from the therapy performed (8.9%) upon tumor progression.

Adolescent↗
PubMed1989

[The immunology of CD4-negative skin hematosarcomas].

A wide panel of monoclonal antibodies to hematopoietic cell differentiation antigens was applied to the study of CD4-negative skin hematosarcomas on cryostat secretions. The most frequent CD4-negative type was the B-cell one identified in 6 of 10 cases. In this type the use of monoclonal antibodies in addition to standard immunoglobulin markers seemed to be very important. T-cell type (CD3+ CD8+), histiocytic variant (CD11+Ia+), pseudolymphoma (polyclonal markers pattern), and Kaposi's sarcoma (hematopoietic cell negative antigen) were identified in 1 case each. The authors emphasize the importance of immunological studies of hematosarcomas because of the high frequency of the generalization of B-cell lymphomas; such studies may also help in the differential diagnosis between pseudolymphoma and hematosarcoma.

Adolescent↗
PubMed1988

[Leukemization of hematosarcomas with primary involvement of the skin].

Clinicomorphological investigation of 81 patients with different types of skin hematosarcomas was conducted. Stage IV of disease was diagnosed in 53 patients, leukemic involvement developed in 18 of them (34%). Leukemic bone involvement was shown to be the main cause of death in myelosarcomas and lymphoblastic lymphomas. There were no significant differences in the frequency of leukemic bone marrow involvement with relation to the site and nature of primary skin involvement. A clinical course of disease was determined by a skin tumor histological type. Some morphological features of bone marrow lymphoblasts in leukemic bone marrow involvement in skin hematosarcomas (irregular cell shape, a moderate nuclear-cytoplasmic ratio, azurophilic granulation, fine-grained patterns of PAS-positive substance) distinguished them from lymphoblasts in leukemic bone marrow involvement in hematosarcomas with a primary focus of another site.

Bone Marrow↗
PubMed1976

[Dermatofibrosarcoma protuberans].

Observations were conducted on 26 patients with protruding dermatofibrosarcoma of the skin. Histological and histochemical characteristics, as well as the mitotic regimen of these tumours, were investigated. Protruding dermatofibrosarcoma is a fibroblastic tumour characterized by a slow growth, usually a big size, persistent multiple reoccurrence, late metastases into the lymphatic nodes and lungs. No regularities concerning periods and frequency of developing of reoccurrences could be established. Clinico-anatomical collations failed to show correlation between the mitotic activity of dermatofibrosarcomas and the degree of their malignancy and rate of growth of the tumour.

Adult↗