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Biomedical subjects

M Zhang

Publications and source records attributed to M Zhang.

At least 541 records · Page 30Linked to original sources
PubMed1999

[Association of HLA-DQA1 and DQB1 alleles with Vogt-Koyanagi-Harada syndrome in Han Chinese population].

OBJECTIVE: To access the association of HLA-DQA1 and -DQB1 alleles with Vogt-Koyanagi-Harada syndrome (VKH). METHODS: The alleles in DQA1 and DQB1 loci of patients with VKH matched with 50 healthy controls were subtyped by polymerase chain reaction-sequence specific primer (PCR-SSP) and PCR-restriction fragment length polymorphism (PCR-RFLP). RESULTS: DQA1 * 0301 and DQB1 * 0401 alleles were in close association with VKH syndrome, as compared to the controls (Pc < 10(-7)), with the relative risk (RR) 16.47 and 88.00, respectively. The frequency of DQA1 * 0301-DQB1 * 0401 haplotype in patients with VKH was also significantly higher than that in healthy controls (78.6%, 4.0%, Pc < 10(-7), RR = 88.00). Among the 15 patients who were negative for the haplotype of DQA1 * 0301-DQB1 * 0401, 7 of them were found to be positive for DRB1 * 15-DQA1 * 0102-DQB1 * 0602/3 haplotype, with the frequency significantly higher than that in the controls (46.7%, 12.5%, Pc = 0.01, RR = 6.13). However, DQA1 * 0103-DQB1 * 0601 was the only one of the DQA1-DQB1 haplotypes with a significantly lower frequency in patients with VKH in comparison with that in the controls (2.9%, 24%, Pc = 0.001, RR = 0.09). The susceptible haplotype was not related to the clinical features. CONCLUSIONS: The results suggest that DQA1 * 0301-DQB1 * 0401 and DRB1 * 15-DQA1 * 0102-DQB1 * 0602/3 be associated with the susceptibility in VKH. On the other hand, DQA1 * 0103-DQB1 * 0601 may play a role in resisting against VKH syndrome.

Adolescent↗
PubMed1999

[A pathological study on the correlation of HPV infection with precancerous cervical lesions].

OBJECTIVE: To study the correlation of cervical condyloma and cancerous lesions with HPV infection. METHODS: Cervical biopsies and histopathological examinations were performed on 179 cases which had abnormal cervical cytological smears. PCR was used to study the HPV-DNA of 128 cases and in situ hybridization (ISH) was used to study 10 cases. RESULTS: 1. Morphologic observations. Most cervical condyloma cases were of the morphologically flat type (97.7%). Two koilocyte types were observed, the classical, or so called diagnostic koilocyte type (39.7%) and the atypical type (60.3%). Cervical condyloma often occurred together with cervical intraepithelial neoplasia (CIN, 42.5%). 2. PCR HPV DNA subtype analysis. Of the 58 cervical flat condyloma, 5 were PHV6/11 positive (8.6%) and 28 were HPV16/18 positive (48.3%). 86.1% of those with atypical koilocyte and 9.1% of those with diagnostic koilocyte had HPV16/18 infection. 66.7% of the lesions in which condyloma coexisted with CIN(2-3) had HPV16/18 infection. CONCLUSIONS: Most cervical condyloma lesions were of the flat type. The appearance of atypical koilocyte is correlated to HPV16/18 infection, which in turn is correlated to the degree of CIN malignancy.

Adult↗
PubMed1999

[Changes in calcium and magnesium levels of lymphocytes in patients with diabetes and hypertension].

To investigate the cellular basis linking non-insulin-dependent diabetes mellitus(NIDDM) and hypertension, we used the atomic absorption spectrometry to detect Ca2+ and Mg2+ levels of serum and lymphocytes in patients with the above diseases. The results were that serum Ca2+ and Mg2+ levels had no change, but lymphocyte Ca2+ level increased obviously and lymphocyte Mg2+ level decreased obviously in both diseases, compared with normal controls. There were no difference between hypertension and NIDDM with and without hypertension. Ca2+ and Mg2+ levels of lymphocytes in patients with hypertension correlated with the systolic pressure respectively(Ca2+, r = 0.4633, P < 0.05; Mg2+, r = -0.4412, P < 0.05), but their levels in patients with NIDDM were not correlated with the fasting blood glucose. The results suggest that there is an abnormal Ca2+ and Mg2+ metabolism in lymphocytes in patients with hypertension and NIDDM.

Adult↗
PubMed1999

[Changes of lipids metabolism during gallstone formation in rabbit model and the effects on these changes by high density lipoprotein preparation injection].

To study the changes of lipids metabolism during gallstone formation and the effects fo the exogenous high-density lipoprotein (HDL) preparation on the formation of gallbladder stone in rabbit model in which the stones were induced by high-cholesterol (HC) diet. The plasma lipids, the bile cholesterol, the activity of plasma lecithine cholesterol acyltransferase (LCAT), the bile acids in both bile and plasma, and the incidence of stone formation were investigated in the control group, HC group, and HC + HDL group (in this group the rabbits were given HDL preparation intravenously regularly). The results showed that the lipids metabolic disorder was characterized by a large amount of cholesterol accumulated in the rabbit's body and when the liver cleaned the accumulated cholesterol, the ratio of biliary cholesterol to bile acids was significantly increased. The latter could cause the production of lithogenic bile. Thought HDL preparation could decrease hepatic cholesterol and increase the level of plasma HDL2-C and the activity of LCAT, it could not lower the incidence of gallstone formation. The results suggest that the HDL preparation had some effects on lipids metabolism, but no significant effect on gallstone formation in rabbit model has been evidenced, so the problem awaits further studies.

Animals↗
PubMed1999

[Polymorphism at cholesteryl ester transfer protein gene loci in patients with gallstone].

This study was designed to reveal the association between the polymorphism of cholesteryl ester transfer protein (CETP) gene loci and gallbladder stone disease. By means of polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLEs) of CETP taqIB, BamHI genes were examined in 104 patients subjected to cholecystectomy for symptomatic gallstone disease and 68 healthy controls. The distribution of CETP taqIB polymorphism in the patients with gallstones differed significantly from that of the controls. The B1 allele and B1B1 jects were more frequently revealed in patients with gallstones (0.523, vs 0.346, 0.288 vs 0.1176, P < 0.05). The B2 allele and the B2B2 jects appeared in the controls more frequently (0.654 vs 0.477, 0.4264 vs 0.242, P < 0.05). The H1 allele, H2 allele and H1H1 genotypic, H2H2 genotypic frequencies of the patients with gallstones were not significantly different from those of the controls (P > 0.05). The results suggest that B1 allele is the major gene in the patients with gallstones, and B2 allele is more frequently revealed in the controls.

Adult↗
PubMed1999

[Isolation and cloning of human beta 1, 4-galactosyltransferase III gene].

Using the conservative nucleotide sequences encoding the catalytic domain of the beta-1, 4-GalT genes in human, bovine, mouse, chick and snail as probes to search the NCBI GenBank EST database, several ESTs with high homology were obtained. Primers were designed in the flanking sequence of EST contig. Using the PCR product amplified in human placenta cDNA library as probe to perform "walking" hybridization with human placenta cDNA library, a cDNA fragment with the length of 1,907 bp was cloned. It contained an open reading frame (ORF) with the length of 1,179 bp, which encodes 393 amino acid residues. The deduced amino acid sequence of this gene shares 43.8% identity to the human beta-1, 4-GalTI and 60.9% in the catalytic domain especially. The expression mapping showed that it was expressed in human most tissues with a single 2.4 kb transcript, but the relative expression level of the transcript are vary. While this gene was mapped on chromosome 1 using the cDNA hybridization with human/rodent hybrid cell line DNA Southern blot panel.

Amino Acid Sequence↗
PubMed1999

[A comparative study of finger plethysmography and aortic pressure for monitoring the effect of external counterpulsation].

Invasive pressure detecting and monitoring of aortic root was conducted in 20 volunteers so as to study the effect of Enhanced External Counterpulsation (EECP) on aortic pressure and finger plethysmography. The results demonstrated that the changes of aortic diastolic pressure linearly related to the counterpusation's bladder pressure, and the aortic augmentation increased to the top of 168.56 mmHg with a D/S ratio of 2.75(amplitude ratio of diastolic waveform and systolic waveform, D/S ratio) following the bladder pressure of 0.45 Kg/cm2. With the increase of bladder pressure, the dp/dt of aortic augmentation elevated gradually, but a down-regulating trend was seen in systolic waveform. During EECP, the starting time of plethysmographic augmentation had a mean delay of 108.20 +/- 25.70 ms comparing with that of aortic augmentation. The duration between the starting time of aortic systolic waveform and that of finger plethysmogram was arround 96.69 +/- 38.70 ms. When the bladder pressure changed, no significant difference was observed among them. The D/S ratio of finger plethysmographic waveform changed in concord with that of aortic pressure with the promotion of bladder pressure. Therefore, a relationship between aortic D/S ratio (Y) and plethysmographic D/S ratio (x) was constructed by the linear regression analysis: Y = 0.376 + 0.457X. It can be concluded that EECP has significant effects on aortic D/S ratio, aortic peak augmentation and its dp/dt value. Finger plethysmography, to date, still plays important roles in monitoring and controlling EECP work safely and effectively.

Adolescent↗
PubMed1999

[Species identification of Mycobacterium tuberculosis with insertion elements].

The Insertion Sequence(IS) element IS6110 and IS1081 of M. tuberculosis complex were used as template to prepare probes for typing strains by means of restriction fragment length polymorphisms (RFLP). Chromosomal DNA of M. tuberculosis H37Ra, H37Rv, M. bovis digested with EcoR I, Pst I, Sal I, BamH I, Pvu II was subjected to Southern Blot analysis With labeled 317bp from IS6110 and 248 bp from IS1081. The results indicated that for M. tuberculosis complex, IS1081 cannot be used to type strains, IS6110 can be used to distinguish strains into broad groups and is an excellent epidemiological tool for studying outbreaks. An investigation of forty-six M. tuberculosis isolates by IS6110 RFLP revealed that M. tuberculosis strains not only are of highly polymorphic, but also are presentin multiple copies ranging from 7 to 18 copies.

DNA Probes↗
PubMed1999

[The effect of granule-rhinitis on allergic nasal mucosa].

OBJECTIVE: To investigate the effect of granule-rhinitis on allergic rhinitis. METHOD: Experimental guinea pigs with allergic rhinitis were treated with granule-rhinitis. During the total course of treatment, sneezing and nasal itching were observed and recorded and then compared quantitatively with those of the control groups. Histological changes of the nasal mucosa were observed. RESULT: The various symptoms of allergic rhinitis were obviously relieved in the treatment group. Granule-rhinitis reduced inflammatory cell infiltration, particularly decreased the number of mast cells and eosinophils in the superficial layers of the nasal mucosa, and reduced the release of mediators from mast cells. It diminished the vasodilatation in nasal mucosa. CONCLUSION: Granule-rhinitis is effective in the treatment of allergic rhinitis.

Animals↗
PubMed1999

[Tissue specificity and expression of a human sperm protein's gene BSD-2.4].

OBJECTIVE: To investigate the tissue specificity and expression in spermatogenic stage of BSD-2.4 cDNA which might be related with fertility and its sequence was received by GenBank. METHODS: Dot blot, Northern blot, and in situ hybridization were used. RESULTS: Northern blot analysis of sixteen human tissues showed that BSD-2.4 cDNA had one transcript only with testis. BSD-2.4 mRNA was detected in germ cells of human testis. CONCLUSIONS: BSD-2.4 is a testis-specific protein. The cDNA was expressed throughout the stages of the spermatogenic cycle.

Animals↗
PubMed1999

[Habitats and present situations of 13 rare precious medicinal materials in Funiu mountains].

The living resources are very abound in the Funiu mountains area because of its special geographical position and climate, and there are also many rare precious living beings and Chinese medicinal herbs, for example, Taxus mairei, Ginkgo biloba, Gastrodia elata, Dendrobium huashanense, Physochlaina infunbularis, Cypripedium japonicum, Dysosma versipellis, Moschus moschiferus, Megalobatrachus davidanus, Succinum, etc, and conscious protection and rational development is an urgent task.

Animals↗
PubMed1999

[The antitumour activity of total saponin of Clematis chinensis].

This paper has shown the cytocidal effects of total saponin of Clermatis chisensis Osbeck (CCS) on experimental tumours EAC cells, S180A cells and HepA cells in vitro, using microculture method 72 hours. The IC50 were 242 micrograms/ml, 193 micrograms/ml and 130 micrograms/ml respectively. In vivo the growth of transplanted mouse tumour(S180) was inhibited by the CCS with inhibitory rates 40.3% (0.25 g/kg), 55.1% (0.5 g/kg), 53.0% (1 g/kg), but CCS(0.2 g/kg, 0.4g/kg, 0.5 g/kg) hasn't effect on survival time of tumour mice(S180A).

Animals↗
PubMed1999

Clinical study of diclofenac sodium eyedrops used before and after cataract operation.

PURPOSE: To determine the effect of 0.1% diclofenac sodium (DS) eyedrops made in China on preventing surgically induced miosis and inflammation. METHODS: Seventy cases of cataract inpatients were randomly divided into two groups. DS eyedrops and normal saline as placebo were applied respectively 3 h, 1 h, 0.5 h before operation and once every morning for 7 days after operation. The pupil diameter was measured five times at different stages during the extracapsular cataract extraction. The eyepain, photophobia, conjunctival injection, KP, aqueous flare and light reaction of pupil were observed once a day after operation for a week. RESULTS: 37 eyes in DS group and 33 eyes in placebo group were included in the study. 32 of 37 eyes in DS group (86.5%) maintained mydriasis in all stages of operation and 33 eyes (89.2%) did not show any obvious sign of inflammation. There was statistically significant difference between the two groups. No serious side effects occurred in the DS group. CONCLUSIONS: The DS eyedrops can prevent surgically induced miosis. Its antiinflammatory effect was significantly better than that of placebo drops.

Anti-Inflammatory Agents, Non-Steroidal↗
PubMed1999

[The study on the structure of 5,10,15,20-tetra-(4-hexadecylpyridiniumyl)porphyrin LB films].

The film-forming properties of 5,10,15,20-tetra-(4-hexadecylpyridiniumyl)porphyrin bromide (TC16PyP) on the air/water interface were studied. LB films of the amphiphilic porphyrin were prepared on the glass, quartz and SnO2 OTE substrates. The morphology and structure of TC16PyP LB films were characterized by scanning tunnelling microscope, UV-Vis absorption spectra, fluorescence spectra and low-angle X-ray diffraction. The experimental results indicated that the amphiphilic porphyrin TC16PyP has good film-forming properties on the air/water interface, its LB films are stable, in TC16PyP monolayer or LB films the long alkyl chains are not straight and the porphyrin macroring is lying nearly parallel to the substrate surface.

Molecular Structure↗