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Biomedical subjects

M Zaviacic

Publications and source records attributed to M Zaviacic.

At least 73 records · Page 4Linked to original sources
PubMed1983

Immunofluorescence and enzyme cytochemical analysis of arterial terminals in the human spleen.

The simultaneous application of immunofluorescent and enzyme cytochemical methods made it possible to analyze the components of the walls of the arterial branches and arterial capillaries, and particularly the spongy arterial terminals in the red pulp of human spleen. The endothelium was identified by alkaline phosphatase demonstration, the basement membranes (BM) by means of antisera to glomerular BM, the media by using the same antisera, and the adventitia by means by antisera to hyaline cartilage, which are presumed to react particularly with adventitial structures. In the spongy arterial terminals visualized by means of anti-hyaline-cartilage antisera, the endothelium and mostly also BM could be detected, yet the enzyme cytochemical reaction to acid phosphatase and alpha-naphthyl acetate esterase and the immunofluorescent reaction to lysozyme yielded negative reactions. The findings suggest that neither macrophages nor other cells containing the given enzymes in detectable form are accumulated in these structures.

Alkaline Phosphatase↗
PubMed1980

Circadian ultrastructural changes in rat gastric parietal cells.

The ultrastructure of rat parietal cells of the gastric mucosa was investigated during the twenty-four hours of a day. Male rats were housed at ad libitum feeding under normal light conditions with dark night. The animals were sacrificed at 6.00 h, 12.00 h, 18.00 h and 24.00 h respectively, in groups of 5 animals after standard 24 hours of starvation. From this material the electronograms of 356 parietal cells (19% in 6.00 h, 26% in 12.00 h, 21% in 18.00 h and 34% in 24.00 h samples) were evaluated. Based on literary data, the parietal cells were specified as secreting parietal cells (S, 38% of the total), secreting parietal cells returning to resting state (SR, 18% of the total), resting parietal cells (R, 35% of the total) and resting parietal cells tending to early secreting state (RS, 9% of the total). Some types of parietal cells are statistically highly significantly (x2 = 130.9, p less than 0.001) unequally distributed during the circadian rhythm: S are less numerous at 6.00 h (2% of the total) and 12.00 h (4% of the total) than expected (7 and 10% respectively), and more numerous at 18.00 h (11% of the total) and 24.00 h (21% of the total) than expected (8 and 13% respectively). Conversely, R are more numerous in the morning (13 and 15% instead of 7 and 9% respectively) and less in evening samples (4 and 3% instead of 7 and 12% respectively). Distribution differences were proved statistically (x2-test) for all cell-cell and hour-hour combinations expect the combinations S-RS and 6.00 h to 12.00 h. The maximal differences in distribution were found to be between the amounts of S and R at 6.00 h and 24.00 h (x2 = 77.3, p less than 0.001) and at 12.00 h and 24.00 h (x2 = 69.3, p less than 0.001). Thus, a distinct circadian rhythm of parietal cells, especially as to their fine cell structures involved in acid production was demonstrated. The results render further evidence of the rhythmicity of gastric acid production in rats.

Animals↗
PubMed1980

Use of arylsulphonic acids for histochemical demonstration of cholesterol and its esters.

A histochemical technique is being described using for the demonstration of cholesterol and its esters a reagent composed of sulphuric acid and 2,5-dimethylbenzene sulphonic acid or p-toluene sulphonic acid dissolved in glacial acetic acid. The results of this method have been compared with other methods for cholesterol identification and verified by extraction and chromatographic techniques. The main advantage of the presented procedure is a better preservation of sections, due to the use of smaller amounts of sulphuric acid, permitting thus a more exact histological localization of cholesterol.

Adrenal Cortex↗
PubMed1980

Computer recognition of gastric (pre)malignancy with the aid of some biochemical, immunological and cytological data.

17 patients with gastric pre-malignancy and 26 without it were correctly classified on the basis of the data from gastric juice (amount, pH, diphenylamine extinction, immunoglobulin A concentration, and total, parietal and surface epithelial cell counts) and from serum (immunoglobulin A concentration) using a kybernetic method of pattern recognition. The most decisive indices appear to be the diphenylamine extinction and immunoglobulin A concentration of gastric juice. Although X-rays, endoscopy and biopsy are the substantial diagnostic methods for gastric (pre-) malignancy a wide screening is desirable to recognize those people who are still asymptomatic but need the special examinations. We propose for this purpose a set of a few simple tests from blood serum and gastric juice, evaluated by method of pattern recognition.

Adult↗
PubMed1979

Effects of feeding a single daily meal and of changes in lighting schedule on the circadian rhythms of oxidoreductases in the rat gastric mucosa.

The present paper investigated the effect of restricted feeding (11.00 to 13.00 h) by a single daily meal upon the activity of various oxidoreductases of the rat gastric mucosa (SDH, alpha-GPDH, LDH, NADHTR), which at ad libitum feeding under normal lighting conditions with a dark night were shown to have distinct circadian activity variations related to the rat's nocturnal food intake preference (Zaviacic and Brozman 1978a). 1st part of the animals received food in 2-h-period by natural daylight (natural lighting conditions), the 2nd part at the same period of time but in the dark (lighting conditions reversed to natural). Checking the consumption of food, we found the animals to become adapted to the new time of food intake beginning with the 6th experimental day. The circadian rhythm of enzymes was examined on days 69, 83, 97, and 104 of limited feeding under the defined lighting schedule, at 06.00 h, 12.00 h, 18.00 h, and 24.00 h in groups of 5 animals. Synchronization of the highest dehydrogenases activities (particularly of SDH) with the time of restricted feeding was seen to develop in the gastric mucosa of the rat. The synchronization effect was more pronounced in the animals which received their food in the dark (and here even more in male than in female). The effect of light during food intake was found to be rather interfering with the development of synchronization of dehydrogenase activities with the time of food intake, and the influence was again more marked in male than in female. The time of food intake was at the feeding schedule restricted to daytime intake the main synchronizer for the circadian rhythm of oxidoreductases of the gastric mucosa of the rat, with the highest daily activities recorded around the feeding time and the lowest activities at night, which is the direct opposite of the circadian rhythm of the same enzymes determined in rats fed ad libitum under normal lighting conditions with a dark night.

Animals↗