Search PubMed⌕ Search

Biomedical subjects

M Ulrich

Publications and source records attributed to M Ulrich.

At least 91 records · Page 5Linked to original sources
PubMed1985

Discovery of a gamma-carboxyglutamic acid-containing protein in human spermatozoa.

Here we describe the identification of a gamma-carboxyglutamic acid-containing protein in human spermatozoa. After thermal decarboxylation the protein is a good substrate for vitamin K-dependent carboxylase from various origins. A quick purification procedure for the decarboxylated protein is presented and in a preliminary characterization we have established its Mr (28 000-30 000) and its amino acid composition.

1-Carboxyglutamic Acid↗
PubMed1985

High voltage electron microscopy of sperm axoneme.

The axoneme of sea urchin spermatozoon has been examined in whole preparation by high voltage electron microscopy. Radial spokes appear arranged in triplets with intratriplet distance of 40 nm, distance between spoke 1 and 2 of 32 nm and between spoke 2 and 3 of 24 nm. Spoke 1 is thicker than others; the complex of the triplets around the 9 microtubule doublets forms a helix of 96 nm pitch. Nexin links connect the A tubule of a doublet with the B of the adjacent one, and also form a helix of 96 nm pitch.

Animals↗
PubMed1984

Release of platelets into the circulation induced by gamma globulin treatment in a case of idiopathic thrombocytopenic purpura.

The kinetics and distribution in vivo of 111In-labelled platelets prior to and during intravenous high-dose gamma globulin therapy in a 3-months-old child with idiopathic thrombocytopenic purpura were evaluated. Concomitantly with a prompt increase in platelet concentration we found evidence for release of platelets into the circulation, presumably to a large extent originating from the spleen and liver. Our results support the concept that, in response to high-dose gamma globulin therapy, platelets are not only forced to remain in but are also released into the circulation, presumably as a result of mobilization of platelets adhering to cells of the monocyte-macrophage system.

Blood Platelets↗
PubMed1984

Interaction of frog virus 3 with the cytomatrix. II. Structure and composition of the virus assembly site.

We have described the structure of virus assembly sites in frog virus 3-infected tissue culture cells based on an examination of sectioned and whole cells by conventional and high voltage (1 000 kV) electron microscopy (HVEM), respectively. We have also attempted to identify the cellular and viral components within the assembly sites using immunofluorescence and a combination of DNase digestion and EM autoradiography. Immunofluorescence studies showed that the sites do not contain tubulin, vimentin, actin, or myosin, confirming EM and HVEM studies which showed the absence of these cytoskeletal filaments from these sites. Enzymatic digestion and autoradiographic studies identified viral DNA in clumps of electron-dense material which are suspended within the matrix of the assembly site. Examination of whole, virus-infected cells by HVEM revealed that the matrix of the assembly site appears as a compaction of the cytoplasmic matrix and appears to be continuous with it. The fine strands of cytomatrix, equated with the microtrabeculae of other cells, are covered with or contain granules measuring 6-8 nm; similar granules also populate the virus assembly sites. Disruption of purified FV 3 with chloroform yielded capsomeres measuring 6-8 nm. Based on all these observations, we postulate that the virus assembly sites are regions of the cytomatrix specialized for virus assembly and that the viral components are transported along the cytomatrix to the assembly sites.

Actins↗
PubMed1983

IgE in leprosy; effect of a Mycobacterium leprae-BCG vaccine.

Since some previous studies have reported elevated total serum IgE levels in leprosy, that may be associated with the existence of a state of generalized T cell anergy, we undertook a carefully controlled study of this immunoglobulin in such patients before and after treatment with a Mycobacterium leprae-BCG vaccine. We found, firstly, that lepromatous leprosy patients suffering active disease had only a moderate elevation of IgE levels that was not statistically significant when compared to appropriate controls. In addition, multiple injections of the vaccine did not cause alterations in the concentration of this immunoglobulin. We were, therefore, unable to confirm the possible existence of a generalized immunodeficiency in lepromatous leprosy that could cause hyper-production of IgE.

BCG Vaccine↗
PubMed1982

Vitamin K-dependent carboxylase in horse liver, spleen and kidney.

The presence of vitamin K-dependent carboxylase is demonstrated in the microsomal fraction of horse liver, spleen and kidney. The carboxylating enzyme systems in the spleen and in the kidney are susceptible to warfarin in a similar way as is carboxylase from the liver. It is concluded, that during the administration of vitamin K-antagonists (anticoagulation therapy) carboxylase in all these tissues is inhibited. Since most probably the majority of the reaction products of spleen and kidney carboxylase are no clotting factors, the inhibition of their production is a side-effect of the anticoagulation therapy. Whether this side-effect is harmful, neutral or even advantageous for the organism in vivo can only be judged, when the function of the various extrahepatic vitamin K-dependent proteins is known.

Animals↗
PubMed1982

Late prognosis in untreated neonatal polycythaemia with minor or no symptoms.

In a series of 635 single non-malformed newborn infants with a gestational age ranging from 35 to 43 weeks we found venous haematocrit values of 60% or greater (range 60-72%) in 117 (18%); 30 neonates (4.7%) had haematocrits above 65%, and of these, 7 had values above 70%. Only 13 developed symptoms, all minor, which might be ascribed to polycythaemia, and all 117 survived. Haemodilution was not performed in any case. Ninety-eight children were followed up at the age of 2.5 years with a physical examination and a Denver Developmental Screening Test. At the age of 6 years an interval story concerning development and health was obtained from a questionnaire in 92 children; 4 children were lost to both follow-up investigations. Of the 113 children, 104 (92%) were classified as normal. The remaining children suffered from febrile convulsions (one case), nocturnal enuresis (five cases), speech defect (one case), gross motor clumsiness (two cases) and hyperkinetic behaviour (one case). None had epilepsy or cerebral palsy. The findings were minor and showed no association to haematocrit levels or possible polycythaemic symptoms in the neonatal period. Hence we do not consider preventive haemodilution to be indicated in neonatal polycythaemia with minor or no symptoms within the studied range.

Female↗