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Biomedical subjects

M Ueno

Publications and source records attributed to M Ueno.

At least 253 records · Page 14Linked to original sources
PubMed1995

A novel 5-HT1-like receptor subtype mediates cAMP synthesis in porcine pial vein.

The 5-hydroxytryptamine (5-HT) receptor subtype mediating 5-HT inhibition of spontaneous rhythmic contractions (SRC) in the porcine pial vein was characterized. Results from pharmacological studies using in vitro tissue bath techniques indicated that the inhibitory effects of 5-HT on SRC were qualitatively and quantitatively mimicked by 5-HT1-like agonists 5-methoxytryptamine (5-MT) and 5-carboxamidotryptamine (5-CT). 5-HT-, 5-MT-, and 5-CT-induced inhibitions of SRC were attenuated in a concentration-dependent manner by methysergide, which yielded similar pA2 values against these three agonists, suggesting that 5-HT, 5-MT, and 5-CT act on the same 5-HT1-like receptors. 5-MT inhibition of SRC was not affected by blocking 5-HT2 (with ketanserin and spiperone), 5-HT3 (with MDL-72222 and ICS-205-930), or 5-HT4 (with ICS-205-930) receptors. Neither was 5-MT inhibition of SRC affected by blocking 5-HT1A (with propranolol and spiperone), 5-HT1B (with propranolol), or 5-HT1C (with ketanserin) receptors. Furthermore, 5-HT and 5-MT inhibitions of SRC were enhanced by cilostazol [a selective adenosine 3',5'-cyclic monophosphate (cAMP) phosphodiesterase inhibitor] and were diminished by KT-5720 (a cAMP-dependent protein kinase inhibitor) but were not affected by M&B-22948 [a selective guanosine 3',5'-cyclic monophosphate (cGMP) phosphodiesterase inhibitor] or KT-5823 (a cGMP-dependent protein kinase inhibitor). Biochemical studies further demonstrated that 5-HT inhibition of SRC in porcine pial veins was accompanied by an increase in cAMP, but not cGMP, synthesis.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1995

Spontaneous transfer of viral protein from membrane of influenza virus-infected cells to liposomes is dependent on the diameter of receiver.

Spontaneous transfer of protein from either influenza virus-infected or uninfected cells to dimyristoylphosphatidylcholine (DMPC) liposomes was examined. The amount of transferred protein of liposomes incubated with influenza virus-infected cells (I-lipo) was more than that of liposomes incubated with uninfected cells (U-lipo). The ratio of the amount of spontaneous transferred protein of I-lipo to that of U-lipo increased in proportion to the diameter of liposomes except for small unilamellar vesicles.

Animals↗
PubMed1995

Study on hepatic artery chemoembolization using temperature-sensitive liposome or lipiodol emulsion.

As a novel method for the medical application of liposomes, we have tried hepatic artery chemoembolization using temperature-sensitive liposomes with hyperthermia for the treatment of hepatic tumors. In this study, the effect of temperature-sensitive liposomes was compared with that of Lipiodol emulsion, which has been used clinically. The temperature-sensitive liposomes, consisting of dipalmitoylphosphatidylcholine or Lipiodol emulsions entrapping doxorubicin, were administered into the hepatic artery of hepatic tumor-bearing rats via a cannula. Doxorubicin administered in a liposomal form showed a high accumulative property toward tumors, with heating, while that in the emulsion form showed a slow release property toward tumors. Not only was tumor growth inhibited, but also, an actual diminishing of the tumor was observed in each form. Side effects were also examined: an abnormal rise in GPT, or necrosis of the normal tissues in liver, which was often observed in hepatic artery chemoembolization using Lipiodol emulsion, was remarkably reduced in the liposomal chemoembolization.

Animals↗
PubMed1995

[Use of semirigid ureteroscopes for treatment of ureteral calculi].

BACKGROUND: The objective of this study is to evaluate the efficacy of ureteroscopic lithotripsy as the treatment of ureteral stones in the recent era by comparing the result using a semirigid ureteroscope with the result using a conventional ureteroscope. METHODS: Between 1991 and 1994, 136 patients with ureteral calculi underwent transurethral ureteroscopic lithotripsy in our hospital. From July 1991 to March 1993, 14F rigid ureteroscope with the rod lens system was used for 82 patients (group 1), whereas from April 1993 to July 1994 either a 9.5F or 7F semirigid fiberoptic ureteroscope was used for 54 patients (group 2). Insertion of ureteroscopes was performed under continuous irrigation of saline without mechanical dilation of the ureteral orifice in both groups. RESULTS: The rates of complete removal of stone at the first trial were 56.1% in group 1 and 72.2% in group 2 with the overall complete removal rate of 62.5%. The complete removal rates of stones in the upper, middle and lower ureters were 18.2%, 54.8% and 72.4% in group 1 and 14.3%, 72.7% and 88.0% in group 2, respectively. The major reasons for unsuccessful treatments was upward migration of stones during the procedure. As intraoperative complications, ureteral disruption occurred in 1 case in group 1, and ureteral perforation in 5 patients in group 1 (6.1%) and 2 in group 2 (3.8%). Acute bacterial infection with prolonged fever was observed in 3 patients in group 1 and in none in group 2. CONCLUSION: From these results, the new semirigid ureteroscope with fiberoptic imaging bundles made the transurethral lithotripsy a safer and more efficient procedure for the treatment of ureteral calculi especially those in the lower ureters.

Adolescent↗
PubMed1995

An ultrastructural study of glomerular basement membrane in rheumatoid arthritis patients with urinary abnormalities.

We measured the thickness of the glomerular basement membrane (GBM) in 48 rheumatoid arthritis (RA) patients with proteinuria and/or hematuria and studied its relationship to clinical features of RA. Ten cases with minor glomerular abnormalities, renal cancer, donor of renal transplantation, were studied as controls. Secondary glomerular diseases and hereditary thin basement membrane disease (TBMD) were excluded. Mean GBM thickness was 289 +/- 74 nm (mean +/- SD) in RA patients, which was significantly thinner than that of control group (342 +/- 38 nm) (p < 0.01). Mean GBM thickness were 276 +/- 72 nm and 336 +/- 68 nm in RA patients with and without gold sodium thiomalate (GST) treatment, respectively (p < 0.05). Mean GBM thickness of RA patients without GST and controls were not different statistically, but RA patients with GST had significantly thinner GBM, compared with controls (p < 0.01). The mean thickness of GBM were also 274 +/- 69 nm and 344 +/- 72 nm in RA patients with and without hematuria, respectively (p < 0.01). According to these results, we suspect that the thinning of GBM in RA patients may be related to GST treatment.

Adolescent↗
PubMed1995

[The renal tubulointerstitium in diabetes mellitus].

Recently, the tubulo-interstitial lesions in diabetes mellitus (DM) have become of greater interest, as well as, glomerulosclerosis. From the view points of tubular function, a variety of changes, including tubular proteinuria and transport of sodium, glucose, and divalent ions have been known until the present time, although the details remain to be elucidated. Morphologically the interstitial fibrosis and the thickening of tubular basement membrane were pointed out to be important features in incipient or overt diabetic nephropathy of insulin-dependent DM patients. We found the expansion of interstitium even in the area without hyalinized glomeruli and atrophic tubules in normo- and microalbuminuric non-insulin dependent DM patients by semiquantitative evaluation of biopsy specimens. Further studies of the mechanisms will be necessary to clarify the mechanism of the development of diabetic nephropathy.

Diabetic Nephropathies↗
PubMed1995

Nephrotic syndrome in a bone marrow transplant recipient with chronic graft-versus-host disease.

A 21-year-old male developed massive proteinuria and microscopic hematuria, 1 year after allogeneic BMT for acute lymphoblastic leukemia. These symptoms occurred during an exacerbation of chronic cutaneous graft-versus-host disease (GVHD). Renal biopsy revealed granular deposits of IgG and IgM along the glomerular basement membrane, and subepithelial electron dense deposits. A diagnosis of membranous nephropathy was made. With prednisolone therapy proteinuria decreased gradually, and amelioration of cutaneous lesions was also noted. It was speculated that the disordered immune regulation of chronic GVHD resulted in the development of immune complex nephritis.

Adult↗
PubMed1995

[Venovenous extracorporeal membrane oxygenation in an elderly patient with severe respiratory failure--report of a case].

A 69-year-old woman, who developed acute respiratory distress syndrome (ARDS) after coronary artery bypass grafting, underwent venovenous extracorporeal membrane oxygenation (V-V ECMO) because conventional ventilatory support was ineffective. We used a covalently bonded heparin surface ECMO system, including an artificial lung, a centrifugal pump, cannulas, tubing and connectors, that was maintained with low-dose systemic heparinization, the patient was weaned from ECMO after 186 hours. During ECMO, her platelet count was about half of the initial level and markedly elevated thrombin-antithrombin complex (TAT), plasmin-alpha 2 plasmin inhibitor complex (PIC) and D-dimer were decreased by the use of heparin and protease inhibitors. V-V ECMO seems to be useful even in patients with severe adult respiratory failure and can be performed safely if a heparin covalent circuit is applied.

Aged↗
PubMed1995

Activation of protein kinase C and the involvement of prostaglandin E2 in the inhibition of osteosarcoma-derived cell alkaline phosphatase activity.

We present evidence for the presence of specific, high-affinity binding sites for tritiated phorbol 12,13-dibutyrate on osteosarcoma-derived (HT-3) cells. Activation of protein kinase C by a phorbol ester resulted in an inhibition of alkaline phosphatase activity and the accumulation of prostaglandin E2. Indomethacin blocked prostaglandin E2 production and enhanced alkaline phosphatase activity. These data suggest that prostaglandin E2 is enhanced by activation of protein kinase C, and in turn, alkaline phosphatase activity is reduced.

Alkaline Phosphatase↗
PubMed1995

A chorioretinal lesion in a patient with Kimura's disease.

A 47-year-old male patient with Kimura's disease showed a white and slightly elevated choroidal lesion surrounded by serous retinal detachment in his left eye. Serological examination revealed esinophilia. Swelling in his left cheek had been increasing during a period of 20 years, and diagnosis of Kimura's disease had been made earlier by biopsy. Fluorescein angiography suggested disturbance of choroidal circulation and dysfunction of retinal pigment epithelium. After corticosteroid therapy, improvement was seen in the fundus findings, the swelling in the cheek and eosinophilia.

Angiolymphoid Hyperplasia with Eosinophilia↗
PubMed1995

Sequence-specific DNA recognition by peptide heterodimers.

The DNA binding motif rich in basic amino acid residues is used by a variety of DNA binding proteins to recognize specific DNA sequences. We report here a sequence-specific DNA recognition by peptide heterodimers derived from the basic leucine zipper proteins. Specific hetero-dimerization is controlled by an artificial dimerization module consisting of a host-guest inclusion complex. Efficiency of the sequence discrimination by the peptide dimers depends on a stability of the half-matched binding complex. However, structures of the matched and half-matched binding complexes differ from one another.

Base Sequence↗
PubMed1995

A quantitative immunocytochemical study of blood-brain barrier to endogenous albumin in cerebral cortex and hippocampus of senescence-accelerated mice (SAM).

The blood-brain barrier (BBB) to endogenous albumin was studied in the cerebral cortex and hippocampus of the senescence-accelerated prone (SAMP8) mouse and senescence-accelerated resistant (SAMR1) mouse strains in corresponding age groups by using a quantitative immunocytochemical procedure. Brain samples after immersion-fixation were embedded at low temperature in Lowicryl K4M and sectioned with an ultramicrotome. Thin sections were exposed to anti-mouse albumin antiserum followed by protein A-gold. Labeling density (gold particles per microns 2) was recorded over four compartments: vascular lumen, endothelium, subendothelial space, and brain parenchyma (neuropil). Morphometric analysis of the electron micrographs revealed that the barrier function of capillaries located in the cerebral cortex of SAMP8 mice was not significantly different from that in control (SAMR1) mice. Nevertheless, in SAMP8 mice, the percentage of leaking microvessels was higher than in control animals. In the older (11-month-old) group of control mice, the percentage of leaking microvessels was higher in the hippocampus than in the cerebral cortex. In SAMP8 mice, however, these differences were negligible. In contrast, in 4-month-old animals, the labeling density of the subendothelial space, which was considered an indicator of albumin escape, was significantly higher in SAMP8 than in SAMR1 mice. Also, the labeling density of the neuropil in the hippocampus of both groups of mice was significantly higher than that in the cerebral cortex. This finding suggests that albumin has an access to the parenchyma of the hippocampus in normal conditions, and that this phenomenon becomes more pronounced during the process of senescence.

Aging↗
PubMed1994

Isolation and characterization of CAJ1, a novel yeast homolog of dnaJ.

A novel member of the Escherichia coli dnaJ family, designated CAJ1, was isolated from a yeast expression library using antiserum against a yeast calmodulin-binding fraction. Although CAJ1 contains neither a Gly-rich region nor a Cys-rich repeat, as are found in other DnaJ relatives, it contains a leucine zipper-like motif.

Amino Acid Sequence↗
PubMed1994

Potentiation of antigen-induced histamine release from rat peritoneal mast cells through a direct interaction between mast cells and non-mast cells.

Rat peritoneal mast cells, which had been sensitized two days earlier by an intraperitoneal injection of rat monoclonal IgE antibodies, were purified by density gradient centrifugation with 60% Percoll (cell purity > 95%). Histamine release from the purified mast cells (PMC) was then compared to that of a non-purified preparation (peritoneal exudate cells; PEC). Both PEC and PMC released similar amounts of histamine upon stimulation with calcium ionophore A23187 and compound 48/80. In contrast, antigen-induced histamine release from PMC was very low compared to that of PEC. PEC released up to 30% of total histamine upon challenge with 1 microgram/ml of antigen, whereas histamine release from PMC was only one third or less than that of PEC. When PEC was suspended in 60% Percoll and treated for a period needed for purification, the reduction of antigen-induced histamine release was negligible. Mast cells purified by centrifugation on a metrizamide gradient released only small amount of histamine similar to Percoll-purified mast cells. Non-mast cells (NMC) recovered from the interface of the 60% Percoll potentiated the antigen-induced histamine release from PMC concentration- and time-dependently. The supernatant of the NMC suspension which was incubated at 37 degrees C for 60 min, however, failed to potentiate histamine release in PMC. We concluded therefore that separation media such as Percoll and metrizamide do not cause the low antigen-induced histamine release in PMC, but that the separation of mast cells from other cells present in the peritoneal cavity itself causes it. Antigen-induced mast cell histamine release is potentiated through a direct interaction between mast cells and NMC, and some cell surface molecules also seem to be involved.

Animals↗
PubMed1994

Cerebral pial arterial innervation with special reference to GABAergic innervation.

Glutamic acid decarboxylase (GAD)-immunoreactive (I) nerve fibers were observed to run parallel to other autonomic nerve fibers, especially vasoactive intestinal polypeptide (VIP)- and calcitonin gene-related peptide (CGRP)-I nerve fibers at the light microscopic level. At the ultrastructural level, GAD-immunoreactivities co-localized with CGRP immunoreactivities in nerve terminals, but not with choline acetyltransferase, VIP, tyrosine hydroxylase and neuropeptide Y immunoreactivities. GAD immunoreactivities were observed in the trigeminal ganglion, some of which were co-localized with CGRP-immunoreactivities. In the proximal portion of the internal carotid artery, GAD-I adventitial ganglion cells were observed and some were also immunoreactive for CGRP. These results strongly suggest that the origin of GABAergic innervation of the major cerebral pial arteries of the cat is mainly in the trigeminal ganglion, and partly in the adventitial ganglia of the internal carotid artery.

Animals↗