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Biomedical subjects

M Ueda

Publications and source records attributed to M Ueda.

At least 1,279 records · Page 71Linked to original sources
PubMed1985

The pectoralis major myocutaneous flap for intraoral reconstruction: surgical complications and their treatment.

The pectoralis major myocutaneous (PM-MC) flap was used for intra-oral reconstruction in 7 patients. Major necrosis did not occur in any of the cases, but minor necrosis did in two cases. In 2 cases an oro-cutaneous fistula was observed. Furthermore, in 2 cases severe facial oedema was experienced, which seemed attributable to circulatory disturbance in the part of the face where the flap was inserted. Muscle atrophy of the flap was observed in greater or lesser degree in all cases. In all cases the pectoralis major myocutaneous flap was used for the purpose of intraoral reconstruction. Intraoral reconstruction involves considerations different from transplantation in other sites. It is the purpose of this paper to present an updated review of our experience with 7 consecutive pectoralis major myocutaneous flaps, including the complications and their treatment.

Adult↗
PubMed1985

Conjugation of poly(styrene-co-maleic acid) derivatives to the antitumor protein neocarzinostatin: pronounced improvements in pharmacological properties.

An anticancer agent of intermediate molecular weight and having both a hydrophilic and hydrophobic nature was developed by utilizing the antitumor protein neocarzinostatin (NCS; Mr = 12000) as a prototype drug. The modification was achieved by reacting the two amino groups on NCS with an anhydride group of partially half-esterified (p-E-) or partially hydrolyzed (p-H-) poly(styrene-co-maleic anhydride) (SMA) in 0.8 M NaHCO3. The SMA samples with narrow molecular weights distributions (Mw = ca. 2000) were prepared by copolymerizing styrene and maleic anhydride in cumene followed by fractionation by means of a column-elution method. The derivatives p-E- or p-H-SMA were then formed by using the appropriate monoalcohols or H2O, respectively. These SMA derivatives contain about 2 mol of anhydride residues/mol of SMA. The reaction product, SMA-conjugated NCS (designated as SMANCS), was purified by dialysis followed by gel filtration with Sephadex G-75. The complete reaction yielded essentially a single product, biantennary SMANCS. The molecular weight of the pure SMAMCS was estimated by various methods, including polyacrylamide gel electrophoresis with NaDodSO4, HPLC in the gel permeation mode, fluorescence polarization, and a decrease in both nitrogen and protein contents. These results agree with the apparent molecular weight of about 16000. Characters of SMANCS was considerably altered from that of parental NCS: solubility characteristics in both organic and aqueous solvents were changed, the biological half-life in blood was prolonged 10 times, and antitumor activity became more pronounced, but the toxicity was reduced to one-fourth of the parental NCS. Thus, the present study has provided a method of improving biologically active substances by polymer conjugation.

Animals↗
PubMed1985

Intrinsic beta-sympathomimetic activity of dilevalol, R, R-isomer of labetalol.

The potency and selectivity of the intrinsic beta-sympathomimetic activity (ISA) of dilevalol were studied with isolated guinea-pig trachea and pithed rat. This drug relaxed the contracture of isolated guinea-pig tracheal preparation induced by histamine in a dose-dependent manner. The dose-response curves of dilevalol and labetalol were shifted to the right with propranolol pretreatment. The relaxing potency of dilevalol was approximately 4.7 times more potent than that of labetalol. Dilevalol markedly reduced the diastolic blood pressure with only a slight increase of heart rate in pithed rats, while isoproterenol and pindolol caused moderate to marked positive chronotropic effects in proportion to their hypotensive effects. These results suggest that dilevalol has more potent ISA than labetalol and shows more beta 2-adrenoceptor selectivity than pindolol.

Adrenergic beta-Agonists↗
PubMed1985

[Pharmacological studies on pinacidil, a new anti-hypertensive agent. 1. Antihypertensive effects in SHR and DOC/saline hypertensive rats].

Antihypertensive effects of pinacidil (PND) were compared with those of hydralazine (HDL) and nifedipine (NFD) by determining the systolic blood pressure of the caudal arteries of conscious SHR and desoxycorticosterone (DOC)/saline hypertensive rats at temperatures between 29 degrees C to 30 degrees C using a newly developed tail cuff method. 1) A dose-dependent antihypertensive effect of PND in male SHR was observed in a dose exceeding 1 mg/kg, p.o. Comparison of antihypertensive effects during 6 hr after oral administration of antihypertensive agents showed that relative potencies of HDL and NFD to PND (1.0) were approximately 0.8 and 0.3, respectively. 2) Although no difference was observed in the antihypertensive effect of HDL between male and female SHR, these effects by PND and NFD were more potent in female SHR. 3) No tolerance was observed in the antihypertensive effect of PND by the once a day oral administration for 5 weeks in male SHR and DOC/saline hypertensive rats. Dose-dependent antihypertensive effects of PND in doses above 1 mg/kg were always observed during experimental periods. 4) In spite of the potent antihypertensive effects, pathological changes such as hyperplasia and hyalinization of arterial walls in kidneys in DOC/saline hypertensive rats were not improved by continuous administration of PND or HDL.

Administration, Oral↗
PubMed1985

[Pharmacological studies on pinacidil, a new antihypertensive agent. 2. Studies on the hypotensive mechanism of pinacidil].

Effects of pinacidil (PND) on the blood pressure of anesthetized cats and contraction of guinea-pig isolated hearts and blood vessels were compared with those of hydralazine (HDL) and nifedipine (NFD). PND showed a dose-dependent hypotensive effect and decrease of total peripheral resistance in a dose above 0.3 mg/kg (p.o.) in anesthetized cats. However, no involvement of the autonomic nervous system was presumed in the hypotensive effect of PND due to studies on autonomic responses. Although negative inotropic and chronotropic effects of PND in the isolated guinea-pig atria and heart (Langendorff method) were slight, PND caused a coronary vasodilation at a dose of 1 micrograms. PND inhibited the norepinephrine (NE) contracture of isolated guinea-pig thoracic aorta and portal vein at 10(-6)-10(-5) M, but concentrations of 10(-5)-10(-4) M were required for the inhibition of K contracture. In the isolated thoracic aorta, HDL markedly inhibited NE contracture, while NFD inhibited K contracture. These results suggest that the hypotensive effect of PND is more closely associated with the inhibition of Ca2+ influx caused by the receptor activation of Ca2+ release from intracellular storage sites than membrane potential-dependent Ca2+ influx.

Animals↗
PubMed1985

Increase of the ventricular fibrillation threshold by 711389-S, a new antiarrhythmic agent, in guinea-pigs.

The increase of the ventricular fibrillation threshold (VFT) by 711389-S, a new antiarrhythmic agent, was compared with several other antiarrhythmic drugs using both in vitro (Langendorff) and in vivo preparations of guinea-pigs. The doses of antiarrhythmic drugs for increasing VFT by 10 volts in in vitro (microgram) and in vivo (mg/kg, i.v.) preparations were as follows: 711389-S: 4.2, 0.15; disopyramide: 32.6, 0.86; quinidine: 32.0, 0.57; aprindine: 7.6, 0.55; propranolol: 16.6, 0.57; and lidocaine: 20.7, 1.52. The duration of the antifibrillatory effects of 711389-S almost corresponded with those of aprindine and propranolol.

Action Potentials↗
PubMed1985

[Control of melanogenesis by glycosylation inhibitors and the inhibitory effect of interferon on melanoma metastasis].

The Glycosylation inhibitors, glucosamine or tunicamycin induced a marked loss of pigment within melanoma cells in addition to their reduced metastatic ability. Electrophoresis of tyrosinase demonstrated the disappearance of or a marked decrease in membrane-bound tyrosinase, T3 in the small and large-granule fractions. Glycoprotein synthesis in the melanogenic subcellular compartments of pigment cells seems to play an integral role in melanogenesis which is principally enhanced in their carcinogenic status. The effect of interferon (IFN) on melanoma metastasis was investigated using B16-F10 melanoma cells. The inhibitory effect was maximal when given 3 h prior to tumor cell inoculation. IFN given 12 and 24 h prior to, as well as simultaneously with, tumor cell inoculation, also reduced metastases, but to a lesser extent. When given 2 h after the inoculation, no effect was shown. The salutary effect of IFN was abolished by anti-asialo GMI, but NK activity was enhanced equally throughout 3 to 24 hrs. This indicates that the effect is substantially dependent on NK cell activity, although the implication of other factors is not excluded.

Animals↗
PubMed1985

Effect of activated lymphocytes on the regulation of hematopoiesis: suppression of in vitro granulopoiesis and erythropoiesis by OKT8+ Ia- T cells induced by concanavalin-A stimulation.

The effects of activated lymphocytes were studied in the regulation of in vitro hematopoiesis. Peripheral blood lymphocytes stimulated by concanavalin A (Con A) were cocultured with normal bone marrow cells in the assay system of hematopoietic stem cells. Con-A-stimulated lymphocytes and their supernatants showed significant suppression of in vitro growth of myeloid and erythroid progenitor cells (CFU-C, CFU-E, and BFU-E). Suppressive activity detected in the T-cell fraction was completely abolished by treatment with OKT3 or OKT8 monoclonal antibody and complement and 20 Gy radiation but not OKT4 or OKIa1 antibody and complement. These observations indicate that peripheral blood lymphocytes can be induced by Con-A stimulation to become suppressor T cells for myeloid and erythroid progenitor cells that are OKT8 positive, Ia negative, and radiosensitive. Together with our previous observation that CFU-C suppressor cells induced by alloantigen stimulation are radioresistant and OKT8- and Ia-positive T cells, it is suggested that in vitro hematopoiesis may be regulated by heterogeneous subpopulations of activated T-lymphocytes.

Antibodies, Monoclonal↗
PubMed1985

Immunological reconstitution after allogeneic and autologous bone marrow transplantation.

Immunological reconstitution was studied with regard to T cell subsets and their functions in patients who received intensive therapy and autologous or allogeneic marrow transplantation. One of the distinct features was the imbalance of T cell subsets. Long-standing inversion of the OKT4/OKT8 ratio was characteristic in both autotransplant and allotransplant patients. Significant differences were observed in recovery of T cell subsets and mitogenic responses between autotransplant and allotransplant patients and between transplant patients and normal controls. In contrast, low reactivities of mixed lymphocyte culture (MLC) recovered to normal levels within 1 yr in both groups of patients. Then the role of suppressor cells was investigated. During early posttransplant periods, MLC suppressor cells were operative in association with the development of low MLC reactivities because suppressor activity was inversely correlated with MLC reactivities at a significant level. Characterization of these MLC suppressor cells revealed that they were an OKT8- and Ia-positive, radioresistant T cell subset of peripheral blood lymphocytes from autologous and allogeneic marrow recipients. These observations suggest that the imbalance of T cell subsets and their abnormal reactivities may by responsible for the development of immunodeficiency and immunodysregulation in bone marrow transplant patients.

Antibodies, Monoclonal↗
PubMed1985

Hyperproduction and gene amplification of the epidermal growth factor receptor in squamous cell carcinomas.

Human squamous cell carcinoma tissue was screened to determine the epidermal growth factor (EGF) receptor level. In 9 out of 15 cases, increased EGF binding was observed relative to normal adjacent tissue. In two tissue samples (SCE1 and SCL1), amplification of the EGF receptor gene and increased mRNA level were also observed. In two other cases (SCE2 and SCL3), EGF receptor gene amplification did not occur. We propose that there is an alternative mechanism for EGF receptor hyperproduction, independent of gene content, active in these tissues. A possible role of EGF receptor hyperproduction is envisioned in human neoplasia.

Carcinoma, Squamous Cell↗
PubMed1985

[Clinical significances of tissue polypeptide antigen in patients with gynecological malignancies].

Recently, tissue polypeptide antigen (TPA) has become of general interest as one of the new tumor-related antigens. In this study, TPA was measured mainly in patients with various gynecological malignancies by radioimmunoassay, and serum TPA levels above 110u/l were considered pathological. The serum TPA level in normal women was 66 +/- 21 u/l (mean +/- S.D.) and the positive rate was 0 percent. Serum TPA levels in patients with cervical cancer, endometrial cancer, ovarian cancer and uterine sarcoma (181 +/- 262, 117 +/- 49, 217 +/- 215, 142 +/- 49u/l) and positive rates (55, 40, 53, 75 percent) were significantly higher than those in normal women. Serum TPA levels in patients with advanced or recurrent diseases had a tendency to show highly positive. In all of 13 patients with positive TPA, serum TPA levels were significantly reduced and turned negative after the appropriate treatments. However, among our follow-up patients, serum TPA levels were often positive even when there was no clinical evidence of recurrence. Conclusion; serum TPA measurement could be a useful subordinate tumor marker in many patients with gynecological malignancies, even though careful judgement is necessary to interpret positive TPA.

Adenocarcinoma, Mucinous↗
PubMed1985

[Changing modality of the treatment in upper urinary tract calculi. Percutaneous nephrolithotripsy and transurethro-ureteral lithotripsy].

From March, 1984 to April, 1985, 70 cases of upper urinary tract calculi (73 renal units) were treated by percutaneous nephrolithotripsy or transurethro-ureteral lithotripsy, mainly utilizing ultrasonic lithotrite. While in the early period of treatment, two stage procedure of creation of nephrostomy tract and percutaneous nephrolithotripsy was performed for renal and upper or middle part of the ureteral calculi, one stage procedure, nephrostomy tract formation followed by ultrasonic nephrolithotripsy is commonly used in recent cases. During this period, conventional stone surgery was seen in 6 cases of pyelolithotomy and 2 of ureterolithotomy, while 52 cases of stone surgery were performed during the earlier period (from January, 1983 to February, 1984). Renal and upper or middle part of ureteral calculi were successfully removed in 49 out of 58 cases (84.5%). Most of the patients required 1 or 2 trials of percutaneous nephrolithotripsy. Middle or lower part of ureteral calculi were removed in transurethro-ureteral approach in 11 of the 12 cases. Hematuria and fever were most common complications after treatment and 3 patients required blood transfusion after 3 or 4 sessions of nephrolithotripsy. Percutaneous nephrolithotripsy and transurethro-ureteral lithotripsy are now widely used for treatment of upper urinary tract calculi, replacing the conventional surgical treatment. Moreover, very recently, extracorporeal shock wave lithotripsy is available in Japan. To the patients with renal calculi indicative of this treatment, both percutaneous nephrolithotripsy and extracorporeal shock wave lithotripsy are introduced and either way of treatment can be chosen by the patient himself.

Adult↗