Search PubMed⌕ Search

Biomedical subjects

M Ueda

Publications and source records attributed to M Ueda.

At least 1,261 records · Page 70Linked to original sources
PubMed1986

[Air scalers].

Explore the source record for details and available documents.

Air↗
PubMed1986

[A study on the cause of urolithiasis of the upper urinary tract--clinical study of risk factors in the formation of stones in the upper urinary tract].

Various risk factors and inhibitors of the stone formation of the upper urinary tract have been pointed out in urine. We examined the amount of daily excretion of several important risk factors (calcium, phosphorus, urate and oxalate) and inhibitors (magnesium and citrate) in the urine of 21 healthy males, 13 male single stone formeks and recurrent and/or multiple stone formers before and after taking the regular diet which contains 500 mg of calcium and 1,000 mg of phosphorus a day. The daily excretion of calcium, phosphorus and magnesium indicated no significant differences among the 3 groups. The excretion of oxalate in urine for 24 hours was significantly decreased in the stone formers after taking the regular diet. The urinary excretion of the urate per body surface area in the stone formers was significantly higher than that in the healthy control. The amount of the excretion of the citrate in urine in the recurrent and/or multiple stone formers was significantly lower than that in the other 2 groups. Many patients of the recurrent and/or multiple urinary stones had more than two abnormal values of above-mentioned risk factors and inhibitors. These results suggest that the causes of the formation of the upper urinary stone were not single but multiple and that the dietary advice to these patients was important against the recurrence of the urolithiasis.

Adult↗
PubMed1986

[Establishment and characterization of TA-4 producing cell line (OMC-1) originating from a human squamous cell carcinoma of the uterine cervix].

A new human cell line designated OMC-1 was established from a metastatic lesion of Virchow lymph node of a large cell non-keratinizing squamous cell carcinoma of the uterine cervix. OMC-1 was successively subcultured 30 times in about 15 months. The monolayer cultured cells appeared to be epithelial with a pavement-like arrangement and tendency to pile up without contact inhibition. Electronmicroscopy showed desmosomes and well-developed tonofilaments. The population doubling time was 43-70 hours, the saturation density was 1.3-1.7 X 10(5) cells/cm2, the plating efficiency was 23-25% and the mitotic index was 3.3-4.8%. Chromosome studies showed aneuploidy and a modal number of 45. After subcutaneous transplantation into nude mice, the cells grew into solid large cell non-keratinizing squamous cell carcinomas. By the flow cytometric analysis, the phase fractions of the cells was G1 + G0 = 40.1%, S = 24.9% and G2 + M = 35.0%. The OMC-1 cells produced TA-4 in culture media. TA-4 was also demonstrated immunohistochemically in the original tumor tissue, cultured cells and tumors in nude mice.

Animals↗
PubMed1986

[A case of central pontine myelinolysis].

A case of central pontine myelinolysis (CPM) following rapid correction of hyponatremia is reported and a review of the literature is made. The patient was a 63-year-old non-alcoholic female who had no liver or kidney diseases in her past history. She was found unconscious after a series of convulsions and was admitted to the hospital. Eighteen months prior to admission, she had a surgery for a ruptured anterior communicating artery aneurysm. Her postoperative course was uneventful except for an urinary incontinence and mild disorientation. She was initially lethargic with conjugate deviation toward right. Nine days after admission, she still remained lethargic, and laboratory studies showed a serum sodium value of 93 mEq/l, serum osmolarity 206 mOsm/l and urine osmolarity 270 mOsm/l when she was clinically diagnosed as having SIADH. She was treated by a strict elimination of water, and administration of sodium, dexamethasone and demeclocycline. In three days, serum sodium was corrected and returned to 137 mEq/l. However, she deteriorated in consciousness and became comatose and developed quadriplegia. CT scans and cerebral angiograms were normal. One month later, another CT scans demonstrated a well-defined hypodensity area in the pons. Brain stem auditory response (BSAR) showed a prolongation of III-V interpeak latency, especially IV-V interval. Her neurological state was essentially unchanged thereafter and she died of septic shock after 12 months' hospitalization. No permission for autopsy was obtained. The clinical course, CT scans and BSAR reported here are indicative of the diagnosis of CPM.

Brain Stem↗
PubMed1986

The effect of immunoglobulin G1 structure on macrophage binding to supported planar lipid monolayers.

We have studied the antibody-dependent binding of macrophages to supported planar lipid monolayers containing haptenated phospholipids (Tnp-Cap-DPPE). Eight monoclonal anti-TNP IgG1s, which had similar affinities to the TNP residues in solution and in the membranes, were used in the experiment. The results showed that mouse macrophages (P388D1 and J774.1) bound with different affinities to these IgG1-coated lipid monolayers. The monoclonal antibody shown to be deficient in macrophage binding was also relatively ineffective in activating complement. These results indicated that individual monoclonal antibodies of a given subclass may prove deficient in terms of the biological activities associated with the group as a whole.

Animals↗
PubMed1986

[Sensitivity to etoposide in cultured cells from cervical squamous cell carcinoma].

The anti-cancer effect of Etoposide was tested in in vitro in cultures of cells taken from a squamous cell carcinoma of the uterine cervix (SKG-IIIb). Growth inhibition was tested by the regrowth assay method, inhibition of DNA synthesis by the uptake of 3H-thymidine, and morphological changes by the method of Limburg et al. The concentrations of Etoposide used were similar to the blood levels recommended for clinical use. The regrowth assay showed that the effective concentration of Etoposide for 50% cell kill was 7.5 micrograms/ml in 2-hour and 1.0 microgram/ml in 24-hour cultures. The 3H-thymidine uptake test showed that a concentration of 13 micrograms/ml for 2 hours or of 3.5 micrograms/ml for 24 hours resulted in 50% inhibition of DNA synthesis. Morphological changes were much greater in the 2-hour cultures at the higher concentration of Etoposide than in the 24-hour cultures at the lower concentration. Investigation of the drug sensitivity by cell kinetics disclosed prolongation of the cell cycle occurring after 96 hours at 1.0 microgram/ml and inhibition of cell cycle progression occurring after 24 hours at 10.0 micrograms/ml and after 4 or 8 hours at 50.0 mu/ml. Thus, the anti-cancer effect of Etoposide on SKG-IIIb depends on both the concentration and exposure time and is related to its ability to inhibit cell growth and DNA synthesis and to cause morphological changes in the cancer cells.

Carcinoma, Squamous Cell↗
PubMed1985

Effects of monensin on insulin processing in adipocytes. Evidence that the internalized insulin-receptor complex has some physiological activities.

In the presence of 10-100 microM monensin (a monovalent cation ionophore), a considerable amount of 125I activity of iodoinsulin accumulated in isolated rat epididymal adipocytes during a 30-min incubation. The accumulation was secondary to the action of monensin to inhibit dissociation of a certain fraction of the cellbound 125I activity. This monensin effect was reversible. The accumulation of 125I activity was ATP dependent and so was the discharge of the accumulated radioactivity. Approximately 91% of the accumulated radio-activity was precipitable with trichloroacetic acid, and at least 84% was reactive to anti-insulin antibody. Monensin at 100 microM appeared to have only mild effects on the cellular activities of glucose transport and cAMP phosphodiesterase. Nevertheless, when cells were first exposed to 10 nM insulin in the presence of 100 microM monensin and then transferred into a hormone-free buffer that contained monensin, the phosphodiesterase activity in cells remained partially activated as if cells were kept exposed to approximately 0.5 nM insulin. Under similar conditions, glucose transport activity remained partially activated as if cells were incubated with approximately 70 pM insulin. Monensin did not inhibit the reversal of the insulin effect per se. Like monensin, 20-100 microM chloroquine (a lysosomotropic inhibitor) induced a considerable accumulation of [125I] iodoinsulin. However, cells that had been exposed to insulin in the presence of chloroquine retained little hormonal effect after washing. Based on these observations and on the reported biological effects of monensin, it is suggested (a) that monensin may induce intracellular accumulation of the insulin-receptor complex by blocking the acidification of endocytic vesicles and (b) that the accumulated insulin-receptor complex may retain a weak, but significant, capacity to stimulate both glucose transport and phosphodiesterase activities.

Adenosine Triphosphate↗
PubMed1985

The origin of metallothionein in red blood cells.

The origin of metallothionein (MT) in red blood cells (RBCs) from a mouse given cadmium was studied in connection with RBC kinetics. Plasma Cd concentration rapidly decreased 3 hr following 109CdCl2 (2 mg/kg, sc) administration, whereas RBC Cd increased from 2 to 4 days, followed by a gradual decrease. RBC Cd was found to be distributed more in the high-molecular-weight fraction than in the MT fraction 12 hr after administration. But, thereafter, Cd increased rapidly in the MT fraction to show changes with time similar to Cd level in RBCs. Hepatic damage induced in a mouse given 21 injections of Cd, with resultant marked elevation of plasma MT concentrations, did not cause any change in RBC Cd concentration. MT was hardly transferred to RBC when a mouse RBC suspension was incubated with mouse hepatic MT. To examine the relationship of Cd-MT and erythropoietic function, mice in the normal group, the phenylhydrazine-induced anemia group (PH), the transfusion-induced plethora group (TR), and the erythropoietin administered plethora group (TR + EP) were given 109CdCl2. Three days after administration, Cd concentration in its RBCs and its MT fraction remarkably increased in the PH group, and was greatly decreased in the TR group. A significant increase was noted in the TR + EP group as compared with the TR group. These results indicate that MT in the RBCs is formed in erythroblasts.

Anemia, Hemolytic↗
PubMed1985

Enhancement of carnitine acetyltransferase synthesis in alkane-grown cells and propionate-grown cells of Candida tropicalis.

The level of carnitine acetyltransferase was markedly increased in harmony with appearance of peroxisomes in alkane-grown cells and propionate-grown cells of Candida tropicalis. From immunochemical studies with antibodies against peroxisomal and mitochondrial carnitine acetyltransferases, it was confirmed that no other type of the enzyme than the peroxisomal and mitochondrial ones was present in alkane-, propionate- and glucose-grown cells of the yeast. The increase in the enzyme level in alkane- and propionate-grown cells was immunochemically proved to result from the increase in the amount of the enzyme protein.

Acetyltransferases↗
PubMed1985

Classification of hearts with overriding aortic and pulmonary valves.

Despite the clarity of the sequential segmential segmental approach to complex congenital heart malformations, the classification of hearts with overriding arterial valves remains contentious. A series of 67 hearts, all with overriding arterial valves, has therefore been studied in an attempt to provide clear and unambiguous criteria for their classification. There were 51 hearts with an overriding aortic valve, 13 hearts with an overriding pulmonary valve and 3 specimens with overriding of both valves. In each of these categories the degree of override and the underlying morphology varied considerably. The options to classify these hearts are limited. Using the "50 per cent rule" as a device to catalogue the type of connexion - irrespective of the morphology - all hearts were described in unambiguous fashion. A comparison with synonyms, as frequently used for purposes of classification, revealed that the latter are often insufficient properly to classify the basic abnormality. By classifying the type of connexion and describing the morphology separately, no basic problem remains in distinguishing between hearts with double outlet right ventricle and subpulmonary ventricular septal defect and hearts with the morphology of Fallot's tetralogy with an aorta almost exclusively arising from the right ventricle. Similarly, the classification of hearts with complete transposition and subpulmonary defect in the setting of the so-called Taussig-Bing heart is brought back to its proper perspective. Proper and consistent application of the sequential segmental approach leaves no room for ambiguity, even in complicated hearts with overriding arterial valves.

Aortic Valve↗
PubMed1985

Morphological characteristics of perimembranous ventricular septal defects and their surgical significance.

The recognition of perimembranous ventricular septal defects and their subcategorization into inlet, outlet and trabecular types is important surgically because of the information it gives concerning the relationship with the atrioventricular conduction bundle. Inlet and outlet defects are well defined. The trabecular defect, in contrast, is less well defined. To obtain a better understanding of the perimembranous trabecular defect we studied 30 hearts, each having a ventricular septal defect known to be perimembranous. Of these 30 hearts, 16 were classified as inlet and 10 as outlet types. The "in between" trabecular defect in each of the remaining four hearts was slit-like, extending from the membranous septum into the area of the medial papillary muscle complex. Each was associated with a complex arrangement of the adjacent tricuspid valve leaflet tissues. In three hearts, leaflets and chords were attached to both the upper and lower rim of the defects. In the other specimen a shelf-like anomalous leaflet attachment was present along the lower rim. The atrioventricular conduction tissues were studied in two of the hearts, showing a close relation between the conduction bundle and the rim of the defect and the anomalous tricuspid valve leaflet attachments. In one case a most unusual disposition was encountered. A fan-like right bundle originated from the main axis at the same level as the origin of the left bundle branches. The anomalous fan penetrated into the connective tissue core of the valve leaflet. The bundle was then traced anteriorly to its usual intramyocardial position. The observed features of perimembranous trabecular ventricular septal defects warrant further caution should they require surgical repair.

Heart Conduction System↗