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Biomedical subjects

M Ueda

Publications and source records attributed to M Ueda.

At least 1,225 records · Page 68Linked to original sources
PubMed1987

A massive dose of vincristine.

An esophageal cancer patient with bilateral lungs and neck lymph nodes metastases received 24 mg of vincristine instead of vinblastine because of the similarity between the two names, and survived multiorgan derangement. Serious states of central and peripheral neuropathy with muscle atrophy, gastrointestinal disorder, bone marrow suppression and mucocutaneous involvement were all encountered. Although hypotension and syndrome of inappropriate secretion of antidiuretic hormone (SIADH) were not observed as vincristine's side actions, toxicity to the myocardium, which has not been documented, was suggested in our case. These toxic impairments, however, subsided clinically within a month, except for paresthesia in the peripheral extremities. The effectiveness of the chemotherapy was remarkable against both the esophageal cancer and the metastatic lesions. No unintentional overdose of a drug, needless to say, should happen, and in order to minimize its possibility, it would be advisable for chemotherapy to be administered only by an experienced physician who is able to check the dose and concentration.

Adult↗
PubMed1987

[Clinical application of the central somatosensory conduction time (CCT)].

We tried to prove the usefulness of CCT as an index of cerebral functions. CCT, regional cerebral blood flow (CBF) and CT scan findings were studied in 213 neurological patients with CVD (66 cases), cerebral tumors (66 cases) and head traumas (81 cases). The technique of CCT recording was modified from that of Jones (1977) using CADWELL 5200 system. CCT was defined as the difference of the peak latency between N13 recorded at the C-7 vertebral electrode and N20 recorded at the somatosensory cortex. The control CCT in normal volunteers (N = 20) was 5.7 +/- 0.4 msec. RCBF was measured with 133 Xe intra carotid injection method of Lassen & Ingvar using gammacamera (DEC GAMM 11/34). CCT tended significantly to become more prolonged in affected hemisphere than in unaffected hemisphere of patients with three groups (CVD, tumor and trauma) (p less than 0.01). Forty percent reduction of control rCBF caused a prolongation of CCT over 7 msec. The degrees of CCT prolongations appeared to be proportional to the degree of rCBF diminution. Prolongation of CCT was remarkable in the side of low density area on CT scan. The prolongation of CCT were revealed in the patients before they did not manifest their neurological deficits especially in the disease of metastatic tumors and glioma. In the case of patients with trauma who we could follow to measure rCBF, CCT tended to become shorten as rCBF increased till normal range. CCT was not significantly prolonged in congenital hydrocephalus.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1986

Identification of the defects in the hemagglutinin gene of two temperature-sensitive mutants of A/WSN/33 influenza virus.

Two temperature-sensitive mutants of WSN influenza virus, ts-61S and ts-134, possess defects in the hemagglutinin (HA) gene. These defects are characterized as a defective intracellular transport of the HA at the nonpermissive temperature and a marked thermolability. The nucleic acid sequences of the HA gene of these two viruses, as well as a series of revertant viruses, were determined. The deduced amino acid sequences demonstrate that the HA of ts-61S varied from the wild type protein by three amino acids while that of ts-134 differed by two residues. For both mutants, analysis of revertant viruses indicated that the phenotype of transport inhibition at the nonpermissive temperature and heat lability were associated with a single amino acid change in the globular portion of the molecule. In the case of ts-61S, the critical change in the HA was the replacement of a serine residue at position 110 with that of a proline. The mutational defect in the HA of ts-134 was due to the substitution of a tyrosine residue at position 159 with that of a histidine residue. Four of five revertants of ts-134 were suppressor revertants, of which some of the compensatory changes did not restore thermostability to the HA.

Amino Acid Sequence↗
PubMed1986

Nonspecific suppressor T cells cause decreased mixed lymphocyte culture reactivity in bone marrow transplant patients.

Decreased reactivity in mixed lymphocyte culture (MLC) was observed in patients within 1 yr after allogeneic and autologous bone marrow transplantation. Suppressor activity of peripheral blood mononuclear cells (PBMC) from transplant patients was studied by adding these cells as modulator cells to a bidirectional MLC with cells from normal individuals. PBMC from transplant patients markedly suppressed MLC reactivity in a dose-dependent manner. Suppressor activity was present in cells forming rosettes with sheep erythrocytes. Treatment of modulator cells with monoclonal antibodies against T cell differentiation antigens (OKT8, OKIa1) and complement completely abolished suppression of MLC. Suppressor activity was unaffected by 30 Gy irradiation. Suppressor activity declined gradually after transplantation and was inversely correlated with MLC reactivity of each patient at a significant level (p less than 0.01). These observations suggest that OKT8+ Ia+ radioresistant suppressor T cells play a role in the development of decreased MLC reactivity observed during the early post-transplant period.

Adolescent↗
PubMed1986

Properties of catalase purified from a methanol-grown yeast, Kloeckera sp. 2201.

Catalase, a marker enzyme of peroxisomes, was purified to homogeneity from whole cells of Kloeckera sp. 2201 (a strain of Candida boidinii) grown on methanol by means of ammonium sulfate fractionation followed by hydroxyapatite, Sephacryl S-300 and DEAE-Sepharose column chromatographies. Crystallized catalase was brown-coloured and needle-like. The molecular mass of the enzyme was about 240 000 daltons consisting of four identical subunits of 62 000 daltons. The minimum size of catalase molecule was estimated to be about 6 X 10 nm from an electron micrograph. Judging from the absorption spectrum, the enzyme seemed to belong to a group of T-type catalase. The Km value of the enzyme for hydrogen peroxide (catalatic activity) was 25 mM, while that for methanol (peroxidatic activity) was 83 mM. Catalase from Kloeckera sp. cells showed a certain degree of similarity to the enzyme purified from alkane-grown Candida tropicalis [T. Yamada et al. (1982) Eur. J. Biochem. 125, 517-521 and 129, 251-255] in its immunochemical properties.

Animals↗
PubMed1986

SEM analysis of surface-treated dialdehydestarch.

Surface characteristics of protein-treated dialdehydestarch (DAS), which is utilizable as a gastrointestinal sorbent for the treatment of uremia, are examined by scanning electron microscopy (SEM). Unique wrinkles or continuous depressions were observed on the surface of some albumin-treated DAS particles. An increase in concentration and composition of albumin to DAS produces more particles with this kind of rugged surface. On the other hand, gelatin treatment provided a DAS with somewhat rough surface. As the stoichiometric ratio of gelatin to DAS increases, some fibrous or thin-leaves-like projections became outstanding on the surface. Urea-binding properties of protein-treated DAS are just about the same regardless of the protein concentration or composition. In addition, wrinkles or depressions partly observed on the surface of albumin-treated DAS are of little benefit in terms of effective urea binding. These results mean that inherent physical properties of DAS such as dialdehyde content would be a critical factor in urea-sorption ability, rather than surface characteristic or the presence of protein.

Absorption↗
PubMed1986

Acute effects of carbon monoxide and cyanide on hepatic mitochondrial function.

The effects of carbon monoxide and cyanide on the hepatic redox state and energy charge were investigated. Rats were used for the experiment under pentobarbital anesthesia. Immediately after laparotomy, a rat was placed in an animal chamber made of a transparent plastic box and exposed to a test gas for 3 min. Every test gas was produced in a gas chamber connected to the animal chamber with a flexible tube. HCN was produced from NaCN and H2SO4. In the CO inhalation experiment, various amounts of CO were introduced into the gas chamber. Immediately after an exposure, about 2 g liver was frozen in situ with a precooled clamp. Oozed blood from the wound surface was sampled. Concentrations of ATP, ADP, AMP, acetoacetate, and beta-hydroxybutyrate in hepatic mitochondria were determined, and the redox state and the energy charge were calculated. For cyanide as well as CO, significant negative correlations were found between the concentration in the blood and the redox state. The same held true for the energy charge. The redox state showed a slight increase at low concentrations of both gases; however, thereafter it began to decrease sharply with increases in concentrations. When concentrations of the toxicant in the blood reached certain levels, a kind of turning point, beyond which the redox state does not decrease any more, was observed. It was about 40% for HbCO and about 2.0 micrograms/ml for cyanide, and the points seemed to be related to the concentrations, beyond which cells are irreversibly damaged. On the other hand, the energy charge did not change at low concentrations. With an increase in toxicant concentrations, the energy charge decreased drastically. The rate of decrease in the energy charge became higher when blood concentrations exceeded certain levels. It was about 40% for HbCO and 2.0 micrograms/ml for cyanide. The presence of low levels of blood cyanide did not affect the relationship between the energy charge and the HbCO concentration.

Adenosine Diphosphate↗
PubMed1986

Time-dependent suppression of melanoma metastases and natural killer cell activation by interferon.

The effect of murine alpha/beta interferon (IFN) on experimental metastasis was investigated using B16-F10 melanoma cells. Since the outcome of metastasis of blood-borne tumor cells is mainly determined within the first 24 h after i.v. inoculation of tumor cells, i.p. injection of IFN was focused on this critical early phase. The inhibition of pulmonary metastases by IFN was found to be maximal when given 3 h prior to tumor cell inoculation, while mice with 24-h and 12-h pretreatment and simultaneous IFN treatment also showed a reduction in metastases, but to a lesser extent. However, mice receiving IFN 2 h after tumor cell inoculation did not show any reduction. Tumor cells cultured for 24 h in IFN-containing medium showed no reduction in metastases. Administration of anti-asialo GMl prior to IFN treatment was found to eliminate the inhibitory effect of IFN 3 h pretreatment. However, natural killer (NK) cell activity in vitro measured at 3 h, 13 h and 24 h after IFN administration was enhanced to the same extent, not paralleling the inhibitory effect on pulmonary metastases. These data indicate that prepared host status against blood-borne tumor cells is established by IFN pretreatment, being maximal when injected several hours prior to tumor cell inoculation, and that this effect is substantially dependent on NK cell activity, though the implication of other factors is not excluded.

Animals↗
PubMed1986

Purification of peroxisomal malate synthase from alkane-grown Candida tropicalis and some properties of the purified enzyme.

Malate synthase, one of the key enzymes in the glyoxylate cycle, was purified from peroxisomes of alkane-grown yeast, Candida tropicalis. The enzyme was mainly localized in the matrix of peroxisomes, judging from subcellular fractionation followed by exposure of the organelles to hypotonic conditions. The molecular mass of this peroxisomal malate synthase was determined to be 250,000 daltons by gel filtration on a Sepharose 6B column as well as by ultracentrifugation. On sodium dodecylsulfate/polyacrylamide slab-gel electrophoresis, the molecular mass of the subunit of the enzyme was demonstrated to be 61,000 daltons. These results revealed that the native form of this enzyme was homo-tetrameric. Peroxisomal malate synthase showed the optimal activity pH at 8.0 and absolutely required Mg2+ for enzymatic activity. The Km values for Mg2+, acetyl-CoA and glyoxylate were 4.7 mM, 80 microM and 1.0 mM, respectively.

Alkanes↗
PubMed1986

Induction of human glioma-specific cytotoxic T-lymphocyte lines by autologous tumor stimulation and interleukin 2.

Two human glioma-specific cytotoxic T-lymphocyte (G-S-CTL) lines were established by autologous tumor stimulation (ATS) with the aid of lectin free interleukin 2 (IL 2). Coculture of patient's peripheral blood lymphocytes and autologous irradiated glioma cells and subsequent addition of partially purified IL 2 enhanced the tumoricidal activity of the lymphocytes. These CTL lines possessed cross-cytotoxic activity against autologous allogeneic glioma cells and exhibited low cytotoxic activity against non-glial tumor cells. They did not lyse autologous lymphoblasts. This phenomenon suggested the existence of a common glioma-specific antigen recognized by the CTL lines. T-cell subset depletion test revealed that the major surface phenotype of G-S-CTL line, responsible for cytotoxic activity was OKT 3 positive, OKT 4 negative and OKT 8 positive. G-S-CTL lines were composed of a low proportion of OKT 8 positive subpopulation after primary ATS and successive propagation with IL 2. The proportion of OKT 8 positive subpopulation was increased by secondary ATS, which enhanced the cytotoxic activity to glioma cells more effectively.

Adult↗
PubMed1986

Double outlet right ventricle: an unusual variant with overriding of both great arteries, absent outlet septum and mitral-to-aortic-to-pulmonary valve continuity.

Double outlet right ventricle constitutes a spectrum of anomalies unified by the fact that both great arteries for their major part arise from the right ventricle. The cases are subcategorized on the basis of the relationship of the great arteries, the precise position of the ventricular septal defect and the muscular morphology of the outflow tracts. The spectrum extends into cases of double outlet left ventricle, while the "in between" cases have been catalogued as double outlet both ventricles. Two hearts are described with overriding of both great arteries, which on morphometric outlet septum and mitral-to-aortic-to-pulmonary valve continuity. To the best of our knowledge, this is the first report of this anatomical arrangement in double outlet right ventricle. Two cases with a similar arrangement have been documented as double outlet left ventricle and double outlet both ventricles. Our two cases further illustrate that the use of the segmental sequential approach to congenital heart disease caters for the most unusual specimens.

Aortic Valve↗
PubMed1986

Increment of prostaglandin E2 in association with elevation of urinary sodium excretion following lead administration.

Lead administered to semi-fasted rats caused an elevation of urinary sodium excretion but no increase of urinary potassium excretion as previously observed in fasted rats. Moreover, in this experiment it was found that urinary and serum prostaglandin E2 (PG E2) contents were increased in association with the elevation of urinary sodium excretion by lead and that natriuresis was suppressed by indomethacine treatment. These findings suggest a stimulation of prostaglandin synthetase rather than to a suppression of the renin-angiotensin-aldosterone system as a primary reaction caused by the lead administration.

Administration, Oral↗
PubMed1986

Revascularization of reversed dermis grafts.

The process of vascularization and the vascular patterns in reversed dermis grafts (RDG) and full-thickness skin grafts (FTSG) in the rat were studied at various intervals after transplantation by a combination of microangiography and histologic techniques. In the early stages the RDG became vascularized more rapidly than the FTSG. However, the inflammatory reaction in the RDG persisted for longer periods than that in the FTSG in the later stages. The prolonged inflammatory reaction in the RDG was presumed to be due to the lack of epidermis for two to three weeks after surgery. Comparatively severe contraction of RDG may result from this prolonged inflammation and the delayed epithelialization.

Animals↗
PubMed1986

Changes in food proteins reacted with nitrite at gastric pH.

Casein and egg albumin were reacted with nitrite at pH 3. The reaction mixtures containing nitrite yellowed when the reaction began; after 24 hours of reaction with 7.5 X 10(-3) M nitrite, about 70% of added casein was sedimented as yellow precipitate. There was a considerable loss of lysine, arginine, tyrosine, and tryptophan residues. This was determined from the amino acid compositions of the proteins obtained by the reaction with 2.5 X 10(-2) M nitrite at pH 3 for 48 hours. An amino acid elution pattern of the treated casein showed five peaks due to new amino acids; three of these were provisionally identified to be cysteic acid, methionine sulfone, and 3-nitrotyrosine.

Amino Acids↗
PubMed1986

Bacterial endocarditis of the aortic valve with septic coronary embolism and myocardial infarction in a 4-month old baby.

A 4-month old baby, who developed infective endocarditis of the aortic valve following purulent arthritis of the hip joint, is presented. The baby developed signs of myocardial infarction and died suddenly at the age of 6 months. Autopsy revealed a localized healed coronary arteritis, almost certainly due to an infected embolus, as the underlying cause.

Aortic Valve↗