Biomedical subjects

M Ueda

Publications and source records attributed to M Ueda.

At least 847 records · Page 47Linked to original sources

PubMed1993

Expression of human erythropoietin in cultured tobacco cells.

Human erythropoietin (Epo) cDNA was engineered for expression in cultured tobacco cells (Nicotiana tabacum L. cv. BY2). Two plasmid DNAs were constructed: pCEP, which contained Epo cDNA under control of the cauliflower mosaic virus-derived 35S RNA promoter and terminator, and pNSEP, which contained signal sequence-deleted Epo cDNA under control of the 35S RNA promoter and terminator. By using the electroporation method, each of these plasmid DNAs was transferred into the protoplasts of BY2 cells together with a plasmid, pNR35, which conferred G418-resistance on the cells. Four G418-resistant clones were obtained from protoplasts transfected with pNSEP and pNR35, and only one of them, named 11N, survived in suspension culture. Integration of pNSEP DNA into the genome of 11N cells was confirmed by Southern blot and PCR analyses. Production of Epo mRNA was shown by Northern blot analysis. Epo protein was shown to be expressed in 11N cells by colorimetric enzyme immunoassay. The productivity of Epo in the 11N cells (1 pg/g of wet cells) was very low.

Amino Acid Sequence↗

PubMed1993

Efficient production of Bacillus stearothermophilus alpha-amylase in Bacillus brevis by altering its signal peptide.

To investigate the role of signal peptides in extracellular production in Bacillus brevis, the Bacillus stearothermophilus alpha-amylase signal peptide was systematically altered and the effects were analyzed in B. brevis. Efficient extracellular production of the amylase in B. brevis was accomplished either by introducing point mutations at positions between -6 and -4 or by replacing the whole signal peptide with that of B. brevis middle wall protein.

Amino Acid Sequence↗

PubMed1993

Studies of cardiovascular responses to some endogenous pressor and hypotensive agents in conscious stroke-prone spontaneously hypertensive rats of different ages.

The effects of the endogenous pressor agents noradrenaline (NA), and angiotensin II (Ang II), and of the hypotensive agents acetylcholine (ACh) and adenosine (ADS), on blood pressure and heart rate in conscious and unrestrained stroke-prone spontaneously hypertensive rats (SHR-SP) and normotensive Wistar Kyoto rats (WKY) of different ages (4-9 weeks old) were investigated. Pressor responses to NA were enhanced in 7- and 9- week-old SHR-SP compared with those in WKY, but pressor responses to Ang II in SHR-SP were not different from those in WKY at all ages. The bradycardias following pressor responses to NA and Ang II were markedly attenuated in SHR-SP, especially older ones. Hypotensive responses to ACH were enhanced in SHR-SP, particularly at 9 weeks of age. However, hypotensive responses to ADS were attenuated in SHR-SP, especially at 7 weeks of age. Transient fall of heart rate due to ADS was also attenuated in 7- and 9- week-old SHR-SP. These alterations of hemodynamic or cardiovascular responses in SHR-SP became more evident in the established stages of hypertension. These results suggest intimate relationships of the enhanced pressor responses to NA, attenuated bradycardias following pressor effects with NA or Ang II, and the attenuated hypotensive responses to ADS with the development or the maintenance of hypertension in SHR-SP.

Acetylcholine↗

PubMed1993

[Obliterative arteriopathy in renal transplantation: an immunocytochemical analysis].

Obliterative arteriopathy is one of the major obstacles to long-term survival of human renal allografts. However, the cellular composition of obliterative arteriopathy has been incompletely characterized. We have performed immunocytochemical investigations of cellular components of obliterative arteriopathy and also investigated immunophenotypic features of proliferating smooth muscle cells in these lesions. Three renal allografts that had been removed due to rejection were available for this study. Time lapse between transplantation and removal of the renal allograft in these patients was 2 months, 5 months, and 4 years and 9 months, respectively. Tissue blocks cut from these renal allografts were fixed in methanol-Carnoy's fixative, and embedded in paraffin. Serial sections from each block were cut and used for histopathological and immunocytochemical studies. Monoclonal antibodies used for this study were as follows: anti-muscle actin antibody, HHF35; anti-smooth muscle actin antibody, CGA7; anti-vimentin antibody; anti-desmin antibody; anti-macrophage antibody, HAM56; and anti-Factor VIII-related antigen antibody. In all the allografts, obliterative arteriopathy was observed in the interlobular and arcuate arteries. In an early stage of proliferative lesions (2 months), the hyperplastic intima consisted largely of macrophages, intermixed with smooth muscle cells. At this stage, smooth muscle cells at the luminal site stained positive with HHF35, but negative with CGA7. In older lesions (5 months, 4 years and 9 months), however, the thickened intima consisted almost entirely of smooth muscle cells. At this stage, smooth muscle cells stained positive with both HHF35 and CGA7.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗

PubMed1993

[A study on cathepsin B-like substance in patients with urological cancer].

Cathepsin B is a lysosomal cysteine proteinase which is thought to regulate intracellular protein metabolism. In the present study, cathepsin B-like activity was determined in the urine of 53 patients with renal cell carcinoma, 22 patients with urothelial carcinoma and 41 control subjects. In addition, immunohistochemical study of cathepsin B was performed in specimens obtained from 20 patients with renal cell carcinoma, 59 patients with bladder carcinoma and 20 patients with renal pelvic and ureter carcinoma by using sheep anti-human liver cathepsin B antibody. Cathepsin B-like activity was higher in the urine from patients with renal cell carcinoma or urothelial carcinoma than in that from controls. Positive reactions for cathepsin B were found in 18 of 20 patients with renal cell carcinoma, in 37 of the 59 patients with bladder carcinoma and in 14 of the 20 patients with renal pelvic and ureter carcinoma. In patients with urothelial carcinoma high rates of positive reaction for cathepsin B were observed in patients with advanced stage tumors, with INF gamma-type tumors and with metastatic lesions. In patients with renal cell carcinoma, there was no correlation between the rate of positive reaction and pathological findings. These results indicate that urinary cathepsin B-like activity is higher in patients with urological cancer and that a highly positive reaction for cathepsin B is a risk factor for tumor invasion, metastasis and poor prognosis in patients with urothelial carcinoma.

Adolescent↗

PubMed1993

Intra- and extraoral prostheses using osseointegrated implants after maxillofacial surgery.

Maxillary and orbital defects due to the resection of maxillary tumors in six cases were treated utilizing maxillofacial prostheses employing pure titanium osseointegrated implants (Brãnemark system). A total of 17 fixtures were installed in the maxillary region, and 16 achieved osseointegration. For the orbital region, nine fixtures were installed, and all fixtures integrated well. Using these fixtures as anchors, four maxillary prostheses and three orbital prostheses were set. The stability of the prostheses were improved by anchors, and the prostheses were highly satisfactory to the patients.

Adult↗

PubMed1993

[Human prostatic tissue specific antigens: a model for the research of monoclonal antibodies against unknown antigens].

Mouse monoclonal antibodies were prepared using a cell mixture containing prostatic secretory, basal cells and stromal cells as an immunogen, and by the immunofluorescence method with cryosections of prostate tissue. An immunohistochemical screening process was used in the expectation that it would help identify not only the antibodies reacting with the secretory cells predominant in the prostate but also those reacting with relatively rare cells in the prostate tissue. Two cell clones have been established which produce antibodies (IgG1) that react well with prostatic secretory cells: a clone producing antibodies specific to prostatic acid phosphatase (PAP) and one producing antibodies specific to prostate-specific antigen (PSA). These antibodies could recognize the native antigens in the seminal plasma, respectively. These findings indicate that antibodies recognizing only one substance can be prepared, even when a mixture of prostatic tissue including many proteases is used as an immunogen. Production of antibodies against prostate-specific antigens other than PAP and PSA by this method should provide a useful means for analyzing unknown antigens in the prostate.

Acid Phosphatase↗

PubMed1993

Immunological abnormalities in HIV-free haemophiliacs.

Since some haemophiliacs manifest profound immunodeficiency with no evidence of human immunodeficiency virus type 1 (HIV) infection, we measured the circulating immune complex (CIC) level in sera obtained from haemophiliacs and addressed the question of whether viral infection is associated directly or indirectly with enhanced CIC production. While more than 90% of HIV-positive individuals had a high level of CIC, around 60% of seronegative ones also showed CIC levels comparable to those of seropositive patients. These sera activated fresh complement in vitro. The patients infected with either HIV or Hepatitis C virus (HCV) or both showed higher frequency and concentration of serum CIC than those free of either pathogens. It is worth noting, however, that 64% of patients with no evidence of infection with HIV or HCV produced significant amounts of CIC. Among the infectious viruses examined, parvovirus is considered as one of the pathogens associated with CIC synthesis, since all the haemophiliacs including the HIV-free patients who had been supplied with heated coagulation factors for several years from birth carried antibodies to parvovirus B19. Strikingly, 60% of the children in this category were positive for CIC, suggesting the possible contribution of parvovirus infection to CIC formation.

Adolescent↗

PubMed1993

Three-dimensional computed tomographic analysis for placement of maxillofacial implants after maxillectomy.

Three-dimensional computed tomography scanning was used to examine six patients who had undergone maxillectomy and who were being considered for endosseous dental implants in the residual maxillary bone. This technique allowed three-dimensional visualization of the precise structures of the deformed maxilla, which facilitated the operation procedure by indicating optimal sites for fixture installation.

Adult↗

PubMed1993

[A study of intercellular adhesion molecule-1 (ICAM-1) in cervical cancer].

Intercellular adhesion molecule-1(ICAM-1), one of the adhesion molecules, plays an important role in target-cell lysis by immune cells. In this study we examined the expression of ICAM-1 in 15 cases of cervical cancer. We also examined ICAM-1 expression on cervical cancer cell lines in response to cytokines. Immunohistochemistry revealed expression (4 positive among 15 cases) of ICAM-1 in cervical cancer. Furthermore, the expression of ICAM-1 on cervical cancer was correlated with the degree of mononuclear cell infiltration, bearing CD3. On the other hand, the expression of ICAM-1 was not correlated with Class I or Class II antigens in cervical cancer. The expression of ICAM-1 on cervical cancer cell lines was augmented by in vitro treatment with interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha. These results suggest that the expression of ICAM-1 on cervical cancer might be modified by cytokines, and ICAM-1 expression on cervical cancer might augment the host immune reaction. So it will be possible to use cytokines in immunotherapy.

Cell Adhesion Molecules↗

PubMed1993

Masticatory improvement using osseointegrated implants: analysis of Japanese patients' responses through questionnaires.

Masticatory improvement obtained by the use of osseointegrated implants (Brånemark System) was analyzed through questionnaires collected from 22 Japanese patients. The questionnaires were designed to assess mastication of different Japanese foods. The postoperative score of masticatory function obtained by analysis of the questionnaires was 90.9, as opposed to the preoperative score of 43.9. A multiple regression analysis indicated that the number of placed implants has no significant effect on the improvement of masticatory function, whereas preoperative performance does. Further, the degree of masticatory improvement showed variation according to the condition of the dental arch opposing the implant prostheses. The group having implant prostheses in the opposing arch showed the highest improvement.

Adult↗

PubMed1993

Pharmacological studies on a new dihydrothienopyridine calcium antagonist. 1st communication: comparative studies on the cardiovascular effects of methyl-4,7-dihydro-3-isobutyl-6-methyl-4-(3-nitrophenyl)thieno[2,3- b]pyridine-5-carboxylate and its two enantiomers in experimental animals.

Antihypertensive effects in conscious spontaneously hypertensive rats (SHR), cardiovascular effects in anesthetized dogs, and calcium antagonistic effects in the rabbit isolated basilar arteries and guinea-pig isolated coronary arteries of S-312 (methyl-4,7-dihydro-3-isobutyl-6-methyl-4- (3-nitrophenyl) thieno [2,3-b] pyridine-5-carboxylate) and its two enantiomers were comparatively investigated. The antihypertensive effect in SHR and hypotensive effect in anesthetized dogs of S-312-d (CAS 120056-57-7) were approximately 2 times more potent than those of S-312, but these effects of S-312-l were very weak even at 10 to 100 times higher doses. Increases of vertebral blood flow in dogs with S-312 and S-312-d were almost corresponding. The IC50 concentrations of S-312-d, S-312, S-312-l in the rabbit isolated basilar arteries contracted with high K+ solution were 1.4 x 10(-10) mol/l, 2.2 x 10(-10) mol/l, and 4.6 x 10(-9) mol/l, respectively. The relaxing effect of S-312-d in the isolated coronary arteries was most potent, followed by those of S-312 and S-312-l. It is concluded that the cardiovascular and calcium antagonistic effect of S-312-d are mainly responsible for those effects of S-312.

Animals↗

PubMed1993

Pharmacological studies on a new Dihydrothienopyridine calcium antagonist. 2nd communication: effects of S-(+)-methyl-4,7-dihydro-3-isobutyl-6-methyl-4- (3-nitrophenyl)thieno[2,3-b]pyridine-5-carboxylate on the 1,4-dihydropyridine binding sites in the brain and on isolated arteries in the Cynomolgus monkey.

The effects of S-312-d (S-(+)-methyl-4,7-dihydro-3-isobutyl-6-methyl-4-(3- nitrophenyl)thieno[2,3-b]pyridine-5-carboxylate, CAS 120056-57-7) on the 1,4-dihydropyridine binding sites using membrane fractions prepared from monkey cerebral cortex, hippocampus, and cerebellum and on isolated monkey arteries were investigated. Specific binding of [3H]-(+)-isradipine was saturable and reversible, and of high affinity. 1,4-Dihydropyridine calcium channel antagonists competed for the binding in the order of S-312-d = nilvadipine = nicardipine > nifedipine. The effect of S-312-d on the binding was due mainly to alterations in the dissociation constant (Kd), without alterations in the binding density (Bmax). In the helical strips of cerebral, coronary, renal mesenteric, and femoral arteries contracted with K+ or prostaglandin (PG) F2 alpha, the addition of S-312-d caused a concentration-dependent relaxation. Relaxant activities of S-312-d in the cerebral arteries contracted with U-46619 (a thromboxane A2 mimetic) or endothelin-1 did not differ significantly from those in the arteries contracted with K+ or PGF2 alpha. Potencies of relaxations induced by S-312-d and 1,4-dihydropyridine calcium channel antagonists in the K(+)-depolarized mesenteric arteries correlated well with those of the binding experiment. Ca(2+)-induced contractions in the mesenteric arteries previously exposed to Ca(2+)-free medium and depolarized by excess K+ were attenuated by S-312-d, whereas PGF2 alpha-induced contractions of the arteries exposed to Ca(2+)-free medium were unaffected.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

PubMed1993

Pharmacological studies on a new dihydrothienopyridine calcium antagonist. 3rd communication: antihypertensive effects of S-(+)-methyl-4,7-dihydro-3-isobutyl-6-methyl-4-(3-nitrophenyl)thieno[2, 3-b] pyridine-5-carboxylate in hypertensive rats and dogs.

Hypotensive and antihypertensive effects of S-312-d (S-(+)-methyl-4,7-dihydro-3-isobutyl-6-methyl-4-(3-nitrophenyl)thieno[2, 3- b]pyridine-5-carboxylate, CAS 120056-57-7) in Wistar Kyoto rat (WKY), spontaneously hypertensive rat (SHR), stroke-prone SHR (SHRSP), and DOCA-salt hypertensive rat (DOCA-HR) were compared with those of other representative calcium antagonists. The minimal effective hypotensive dose of S-312-d in WKY was 3 mg/kg p.o. and those in SHR, SHRSP, and DOCA-HR were 1 mg/kg p.o. in gum arabic suspension. The minimal antihypertensive dose of S-312-d in polyethylene glycol solution was 0.3 mg/kg p.o. in SHRSP. The antihypertensive effects of S-312-d was the most potent and long-lasting compared with the calcium antagonists, nifedipine, nicardipine, nimodipine, nilvadipine, and flunarizine. In conscious two-kidney Goldblatt-type hypertensive dogs, a significant antihypertensive effect and concomitant increases of heart rate with S-312-d at 1 mg/kg lasted for 4 to 6 h after oral administration. Determination of the plasma concentration of S-312-d by HPLC showed that more than 4.3 ng/ml of S-312-d is required for a significant antihypertensive effect. Subcutaneous administration of atenolol at 20 mg/kg 30 min before S-312-d significantly inhibited the tachycardia with S-312-d at 1 mg/kg p.o. but not its antihypertensive effect. S-312-d is considered useful for the treatment of essential hypertension and related organ disorders.

Animals↗

PubMed1993

Pharmacological studies on a new dihydrothienopyridine calcium antagonist. 4th communication: prophylactic and therapeutic effects of S-(+)-methyl-4,7-dihydro-3-isobutyl-6-methyl-4-(3-nitrophenyl)thieno[2, 3- b]pyridine-5-carboxylate in stroke-prone spontaneously hypertensive rats.

The prophylactic and therapeutic effects of S-312-d (S-(+)-methyl-4,7-dihydro-3-isobutyl-6-methyl-4-(3-nitrophenyl)thieno[2, 3- b]pyridine-5-carboxylate, CAS 120056-57-7) were compared with those of nimodipine or nicardipine using male stroke-prone spontaneously hypertensive rats (SHRSP). The survival rate of SHRSP was dose-dependently increased by once a day oral administration of S-312-d (0.3, 1, and 3 mg/kg) or nimodipine (10 mg/kg), while all non-treated SHRSP fed with high Na+ diet died within 40 days after the start of the experiment. All SHRSP treated with 3 mg/kg S-312-d survived during the 60-day experiment periods. Marked decreases of body weights and various neurological symptoms were also inhibited with S-312-d or nimodipine. Moderate diuretic effects were observed with S-312-d at doses of 1 and 3 mg/kg. The appearance of urinary occult blood in control SHRSP was markedly inhibited with S-312-d at 1 mg/kg and nimodipine at 10 mg/kg. Histological examination of the brain of SHRSP showed that cerebral stroke lesion including edema, hemorrhage, and/or softening was dose-dependently inhibited with S-312-d. Once a day oral administration of S-312-d (1, 3, or 10 mg/kg) dose-dependently increased the body weights and improved the neurological symptoms of diseased SHRSP. The appearance of proteinuria and of occult blood in the urine of SHRSP were also markedly inhibited with S-312-d or nicardipine. Histological examination of the brain of SHRSP showed that the arbitrary neurotoxic index (ANI) for stroke lesion dose-dependently decreased with S-312-d at 1, 3, and 10 mg/kg as follows: 4.8, 3.0, 2.3. The ANI for non-treated SHRSP was 7.6. The therapeutic effects of nicardipine (ANI 3.9) at 10 mg/kg corresponded to those of S-312-d at 3 mg/kg. Thus, S-312-d can be recommended for the treatment of cerebral insufficiency or vasospasm following stroke as well as in essential hypertension.

Animals↗

PubMed1993

Decreased expression of human melanoma-associated antigen ME491 along the progression of melanoma pre-canceroses to invasive and metastatic melanomas.

ME491 expression was examined by immunohistochemistry in the different stages of various types of human malignant melanomas. In addition, the primary and metastatic lesions in five patients were studied to compare the ME491 antigen expression between these lesions in a single patient. A large proportion of cells from melanoma pre-canceroses showed strong ME491 expression, whereas the proportion of negatively stained tumour cells increased in the melanomas invading the dermis and in metastatic melanomas. In addition, a comparison of five sets of primary tumour and its metastatic lesion revealed decreased expression of this antigen in metastases. These results show that the reduction or loss of ME491 antigen expression is associated with increased invasiveness and metastatic ability of human malignant melanoma, suggesting that ME491 may act as an anti-metastatic gene.

Adult↗

PubMed1993

Prosthodontic closure of palatal fistula with osseointegrated implants and onlay bone grafts--case report.

A case treated with onlay bone grafting and simultaneous osseointegrated implant insertion is reported. The patient had an oronasal fistula in the center of the premaxillary region due to failed repair of a bilateral cleft palate. Four fixtures were inserted with onlay bone grafting of the maxillary alveolar ridge. Twelve months postsurgery, one fixture was lost as a result of severe bone resorption around the fixture, but three fixtures integrated strongly. We constructed an overdenture stabilized with ball attachments connected to the implants 13 months after the first operation. Speech and masticatory function improved remarkably with closure of the fistula and good fixation of the denture.

Adult↗

PubMed1993

Cervicofacial actinomycosis: report of two cases.

This article presents two cases of actinomycosis. Case 1 was a 39-year-old man who was first seen with the chief complaint of swelling around the left submandibular region. Case 2 was a 40-year-old woman who was first seen with the chief complaint of mass formation around the left buccal area. Both cases were initially diagnosed as malignant tumors and were later histopathologically interpreted as actinomycosis because of the presence of sulfur granules.

Actinomycosis↗