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Biomedical subjects

M Uchida

Publications and source records attributed to M Uchida.

At least 397 records · Page 22Linked to original sources
PubMed1987

Inhibitory effects of calmodulin antagonists on isoproterenol- and dibutyryl cyclic AMP-stimulated amylase release from rat parotid acinar cells.

The effects of three calmodulin antagonists on rat parotid amylase release were investigated in vitro using a dispersed acinar cell preparation. The potent calmodulin antagonists, trifluoperazine (TFP) and N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), inhibited both 1 microM isoproterenol (ISO)- and 1 mM dibutyryl cyclic AMP (DBcAMP)-stimulated amylase release in a dose-dependent manner at concentrations of 25-100 microM. The IC50 values for the ISO-stimulated amylase release were 22 microM with TFP and 42 microM with W-7, and the values for the DBcAMP-stimulated release were 25 microM and 48 microM, respectively. The weak calmodulin antagonist, N-(6-aminohexyl)-1-naphthalenesulfonamide (W-5), caused only slight inhibition at a concentration of 100 microM. These calmodulin antagonists only had a very small effect on the spontaneous release of lactate dehydrogenase. The results suggest that the calcium-calmodulin system may play a role in the exocytotic process of amylase release from the rat parotid gland.

Amylases↗
PubMed1987

Relationship between translobular migration in perfused rat liver and hydrophobicity of dialkyl dithiolanylidenemalonates and related compounds.

The translobular migration of five dialkyl dithiolanylidenemalonates and five related compounds in perfused rat liver was determined and related with the hydrophobicity, log P. In the elution profile from the perfused rat liver, the peak position (elution volume) varied from 1.5 ml for dimethyl dithiolanylidenemalonate to 186 ml for the dibutyl analogue, dependent on the length of the alcoholic alkyl groups or the hydrophobicity of the compounds. An excellent correlation exists between--log (elution volume) and log P. The hydrophobic interaction with sinusoidal components seems to retard the compound migration from the periportal zone to the hepatic vein. Addition of rat blood (5% v/v) to the perfusion Tyrode solution accelerated the migration of hydrophobic compounds resulting in a gentler slope of the correlation plot. A similar correlation with log P was observed for translobular migrations of other xenobiotics such as naphthalene, nitroanisole, dichlorobenzenes and parathion in rat liver perfused with autologous blood.

Animals↗
PubMed1987

[The significance of chemotherapy in oral cancer].

Fifty-three patients with postoperative recurrent epidermoid carcinomas of the head and neck were treated with BEAM, DP, MBD and CAP regimens. Complete response (CR) and partial response (PR) were achieved in 9% and 23% of all evaluable patients, respectively. Especially, in patients with oral cancer (17 cases), the response rate was only 23% (CR 0%, PR 23%). From our results, it was concluded that chemotherapy in any form must be palliative for a patient who has received prior unsuccessful radiotherapy or surgery. For previously untreated patients who present with Stage III or IV advanced carcinoma, combination chemotherapy with CDDP, MTX and PEP should be considered as the initial method of treatment. Adjuvant chemotherapy and intra-arterial chemotherapy for oral cancer should also be considered. Adjuvant chemotherapy combined with UFT and MTX is indicted to be effective for the prevention of local recurrence and micrometastases, but a further randomized study is necessary to confirm these findings.

Adult↗
PubMed1986

Is phospholipid a required cofactor for the activity of mammalian signal peptidase?

To determine whether phospholipid is required for the activity of mammalian signal peptidase, the enzyme was partially purified from porcine pancreas and then extensively freed of phospholipid by SP-Sephadex C-50 chromatography. The delipidated enzyme showed signal peptidase activity, with a low concentration of detergent. Phospholipid was found to release the enzyme from the inhibition due to excess detergent.

Animals↗
PubMed1986

Volar dislocation of the metacarpophalangeal joint of the thumb--a case report.

A patient with a volar dislocation of the metacarpophalangeal joint of the thumb is reported in which closed reduction proved impossible because of the interposition of the dorsal capsule, extensor pollicis longus tendon and extensor pollicis brevis tendon in the metacarpophalangeal joint. The collateral ligament was also ruptured. Therefore open reduction is recommended for similar cases.

Female↗
PubMed1986

Long-term investigation of habitual tonsillitis in children.

The authors were in close contact with children who had indications for tonsillectomy and investigated them in both acute tonsillitis and intermittent periods of tonsil inflammation. The results obtained were as follows: Repeated attacks of acute tonsillitis clearly decreased in number. No secondary diseases due to tonsillitis were observed. When repeated attacks of acute tonsillitis occurred, the symptoms were not as serious as previously.

Adolescent↗
PubMed1986

Immunological reaction to horseradish peroxidase of the palatine tonsil of the rabbit.

Detection of the immune response to external antigen of the palatine tonsil of the rabbit is an interesting problem. Employing horseradish peroxidase (HRP) as an external antigen, the reactions of a perfusion sensitized group and those of a group sensitized by intravenous injection were examined. The results were as follows: 1. Hypertrophy of the palatine tonsil was recognized only in the perfusion sensitized group. 2. The serum titer level of the antibody to HRP was recognized to be high in the intravenous injection sensitized group, which was confirmed by the production of antibody in the spleen. It was also confirmed in the repeated perfusion sensitized group at a low level. 3. Antigen invasion through the epithelium of the crypt was possible by repeated intravenous injection sensitization as for perfusion sensitization. Also, invading HRP was observed electron microscopically to be phagocytized by macrophages and dendritic reticular cells. 4. In the perfusion sensitized group, production of antibody was not found in the palatine tonsils but in the regional lymph nodes of the neck. 5. In the perfusion sensitized group, a large number of eosinophils were noted mainly on the basal side of the interfollicular zone. 6. In the intravenous injection sensitized group, anti-HRP was found in the palatine tonsils and also in the spleen. Participation of the tonsils has been revealed in general human immunity.

Animals↗
PubMed1986

Altered glomerular localization of heparan sulfate proteoglycan in experimental nephritides.

The distribution of heparan sulfate proteoglycan (HS-PG) was examined electron microscopically by the high iron diamine (HID) method in puromycin aminonucleoside (PAN) nephrosis, accelerated Masugi nephritis (NTN), and serum sickness nephritis induced by bovine serum albumin (BSA nephritis) in the rat. In PAN nephrosis rats, no change was observed in the distribution of HS-PG in the lamina rara externa (LRE) of the glomerular basement membrane (GBM) throughout the experiment. In NTN rats, however, the loss of HS-PG was observed, and it was associated with subepithelial electron dense deposits formed possibly by serum sickness mechanism, but not with inflammatory cell infiltration. In BAS nephritis, immune deposits were seen in mesangial, subendothelial, intramembranous and subepithelial areas. The deposits in the former three areas seemed to have little reciprocity with the loss of HS-PG and proteinuria. Urinary protein increased in accordance with the development of subepithelial deposits and the loss of HS-PG in the area of the deposits in the LRE. These results indicate that HS-PG could be preserved even in marked proteinuric states in morphologically intact basement membrane, but altered and lost distribution of HS-PG associated with subepithelial immune deposits could in turn result in the development of proteinuria.

Animals↗
PubMed1986

Neutrophil adhesion abnormality with deficient surface membrane proteins (gp 110 and p 98): the effect of their antibodies on the function of normal neutrophils.

A sister and brother previously described with neutrophil adhesion defects and a lack of two neutrophil membrane proteins, glycoprotein with a molecular weight of 110 K and 115 K, were further studied. Rabbit polyclonal IgG antibodies were raised against neutrophil membrane proteins of approximately 110 K from normal individuals, and absorbed with the siblings' neutrophil membrane proteins. The antibodies thus absorbed reacted with two normal neutrophil membrane proteins, a 110 K glycoprotein and a 98 K protein, which were totally deficient in the siblings' neutrophils. The polyclonal antibodies were also reactive with a 95 K membrane protein of normal lymphocytes. The protein was missing in the siblings' lymphocytes. Three membrane proteins, Mac-1, LFA-1, and p 150, 95 each consisting of a specific alpha-subunit and a beta-subunit common to them, have been reported to be deficient in neutrophils from several patients with a neutrophil adhesion disease. Using monoclonal antibodies to Mac-1 alpha, LFA-1 alpha, and to the beta-subunit, it was deduced that the siblings' neutrophils lacked the above three membrane proteins. The disease in the siblings is thus identical with that previously described. Sodium dodecyl sulfate polyacrylamide gel electrophoresis of immunoprecipitates, formed by reacting labeled normal neutrophil membrane proteins with our polyclonal antibodies, revealed three bands, almost identical with that of immunoprecipitates formed with the anti-beta-subunit monoclonal antibody. This finding indicates that the polyclonal antibodies reacted mainly with the beta-subunit of the membrane proteins.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1986

Acceleration of liver regeneration by malotilate in partially hepatectomized rats.

The effect of malotilate (diisopropyl 1,3-dithiol-2-ylidenemalonate) on liver regeneration was studied by using partially hepatectomized rats. Malotilate administration (100 mg/kg/day, p.o.) facilitated the weight gain of the liver after partial hepatectomy. Protein, RNA and DNA contents of the regenerating liver correlated well with the weight gain. The weight gain, RNA and DNA contents, and mitotic index were significantly suppressed in the alloxan-diabetic rats 24 hr after partial hepatectomy. However, malotilate administration significantly improved the delayed recovery of RNA content. Other parameters were not significantly improved by malotilate, but tended to increase to a level comparable to those of partially hepatectomized control rats. These results show that malotilate accelerates cell proliferation, resulting in facilitated liver regeneration in rats (as well as in alloxan-diabetic rats).

Alloxan↗