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M Tuszewski

Publications and source records attributed to M Tuszewski.

At least 19 recordsLinked to original sources
PubMed1997

Nested polymerase chain reaction strategy simultaneously targeting DNA sequences of multiple bacterial species in inflammatory joint diseases. I. Screening of synovial fluid samples of patients with spondyloarthropathies and other arthritides.

OBJECTIVE: Bacteria play a crucial pathogenetic role in Lyme arthritis (LA), reactive arthritis (ReA), other forms of spondyloarthropathy (SpA), and possibly in undifferentiated oligoarthritis (uOligo). Polymerase chain reaction (PCR) technology has been applied to detect bacterial DNA of individual microbes in synovial fluid (SF) of patients with arthritides. We screened for DNA sequences of 8 bacterial species simultaneously in SF of patients with inflammatory joint disease. METHODS: We examined 104 SF samples of 96 patients with ReA (n = 13), undifferentiated SpA (uSpA, n = 10), uOligo (n = 50), juvenile chronic arthritis (JCA, n = 13), and rheumatoid arthritis (RA, n = 10). A nested PCR approach was developed to detect DNA sequences of 8 bacteria: Chlamydia trachomatis, C. pneumoniae, Yersinia enterocolitica, Salmonella enteritidis, Campylobacter jejuni, Shigella flexneri, Klebsiella pneumoniae, and Borrelia burgdorferi. The detection limit was determined at 10 bacterial/sample. Serology and lymphocyte proliferation assay were done in parallel in most patients. RESULTS: In 12 cases bacterial DNA of B. burgdorferi (n = 7), C. trachomatis (n = 2), C. jejuni (n = 2), and C. pneumoniae (n = 1) was detected in patients with uOligo (n = 9) and JCA (n = 3), while no evidence of bacterial DNA was found in patients with ReA, uSpA, and RA. Shigella flexneri DNA was detected in 4 cases, but the significance of this finding remains uncertain due to the high sequence homology of this species with Escherichia coli. DNA of Y. enterocolitica, S. enteritidis, or K. pneumoniae was not found. A positive serologic response was found in 7/9 PCR positive patients. In 11/96 cases antibodies to 2 or more bacteria were found in parallel (11.5%). Antigen specific lymphocyte proliferation was observed in 5/9 PCR positive patients. CONCLUSION: Bacterial DNA was detected in peripheral joint of patients with uOligo and JCA, but not in ReA, uSpA, or RA in this study. The detection of bacterial DNA in synovial material by PCR technology gives useful diagnostic information, especially when antibodies against several microbes are present or antibodies are not detectable. Failure to detect bacterial DNA in patients with ReA and uSpA with longstanding disease suggests that in later stages autoimmune mechanisms may operate.

Adolescent↗
PubMed1997

Nested polymerase chain reaction strategy simultaneously targeting DNA sequences of multiple bacterial species in inflammatory joint diseases. II. Examination of sacroiliac and knee joint biopsies of patients with spondyloarthropathies and other arthritides.

OBJECTIVE: Bacteria play a crucial pathogenetic role in reactive arthritis (ReA) and other forms of spondyloarthropathy (SpA) and in Lyme arthritis. Although there is evidence of local persistence of bacterial antigens no definitive method revealing microbes in peripheral joints has been established. We detected DNA of individual bacteria in synovial material by PCR. Applying molecular technology we screened simultaneously for 8 bacterial genomes in arthritis and sacroiliitis. METHODS: Sacroiliac (SI) biopsy specimens taken from the SI joint of 8 patients with ankylosing spondylitis (AS, n = 5) and undifferentiated SpA (uSpA, n = 3) by computed tomography guided biopsy were investigated for presence of bacterial DNA. Similarly, synovial membrane samples obtained by office arthroscopy from 15 patients with ReA (n = 5), uSpA (n = 3), undifferentiated oligoarthritis (uOligo, n = 3), and rheumatoid arthritis (RA, n = 4) were screened. Nested PCR was performed for DNA of the following bacteria: Chlamydia trachomatis, C. pneumoniae, Yersinia enterocolitica, Salmonella enteritidis, Campylobacter jejuni, Shigella flexneri, Klebsiella pneumoniae, and Borrelia burgdorferi. RESULTS: No bacterial DNA was found in the SI biopsies of patients with uSpA and AS. DNA of B. burgdorferi (n = 2) and C. trachomatis (n = 1) was detected in 3 patients with uOligo, but not in patients with ReA or RA. DNA of other microbes including K. pneumoniae was not found. Patients' mean disease duration was 5.3 years (2 mo-8.4 yrs). CONCLUSION: We found bacterial DNA in peripheral joints of patients with uOligo, while in patients with ReA, AS, and uSpA no bacterial DNA was detected in peripheral or SI joints. The failure to detect bacterial DNA in patients with SpA suggests autoimmune mechanisms operate in later stages of disease.

Adult↗
PubMed1991

[Comparative evaluation of tensile strength of absorbable threads Dexon and Maxon. Experimental studies].

This paper submits strength tests on Dexon and Maxon resorbable sutures at the knot and without a knot, the breaking resulting from hydrolysis. The tests were performed on rats, by comparing Maxon monofilament suture with Dexon woven suture. The dissections were done 3, 7, 21 and 42 days after the implantation. What we found out was a remarkably slower process of strength loss displayed by Maxon sutures in time after the implantation. After 21 days, Dexon sutures displayed a minimal strength value, whereas Maxon sutures still retained ca 60% of the initial properties.

Absorption↗
PubMed1985

Long-term results of jejunoileostomy for extreme obesity.

The results of jejunoileostomy performed on 130 extremely obese patients in 1972-1982 are presented. The mean preoperative body weight was 130.9 kg. The postoperative observation period was 2-10 years and in 85 cases it was more than 5 years. The Salmon-Scott technique of jejunoileostomy was used in 6 cases and the Payne-De Wind method in 124. Overweight was reduced from 118% to 30.4% of normal body weight (Broca Index). Weight reduction was classified as very good or good in 82% of the patients. It was accompanied by increased psychosocial activity and improved working capacity. Disturbed carbohydrate and lipid balances normalized and liver steatosis disappeared. The direct postoperative mortality was 3.8%, and another patient died from long-term metabolic complications. Clinical symptoms of hepatic insufficiency appeared in one patient and were successfully managed with conservative treatment. Most other metabolic disturbances were transient and without clinical manifestations. They gradually subsided as adaptation progressed in the active intestinal remnant. Follow-up studies 5 to 10 years after jejunoileostomy showed that its therapeutic effects were lasting and the patients were free from persistent metabolic disturbance. In the authors' opinion, jejunoileostomy is an effective therapeutic method, though associated with certain risks.

Female↗
PubMed1984

[Morphological assessment of the adaptation process in the small intestine after jejunoileostomy for extreme obesity].

The paper presents the author's experiments performed for examining the morphological adaptation in the intestinal loop, preserved for the food passage after jejunoileostomy for extreme obesity. Macro-, micro-, and ultramicroscopic examinations were carried out in 20 patients. A characteristic elongation and hypertrophy of the active intestinal loop, thickening of its wall (especially mucosa), hypertrophy and elongation of the intestinal villi as well as reduction of the mucous cells could be observed. The enzymatic activity of the mucous membrane could be found to be increased in the active part of the jejunum too. In the author's opinion the observed changes clearly demonstrate the transient character of those metabolic disturbances which develop after producing the artificial short gut syndrome.

Adaptation, Physiological↗