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Biomedical subjects

M Tsukamoto

Publications and source records attributed to M Tsukamoto.

At least 199 records · Page 11Linked to original sources

Stroke mortality and proportional expenditure on selected food items in Japanese communities.

Proportional food expenditure was calculated from the 1959 National Survey of Family Income and Expenditures and 843 municipalities were trichotomized according to the relative expenditure of various food items. Sex specific standardized mortality ratio (SMR) of stroke (1969-83) was calculated for each trichotomized food expenditure subgroup and compared. Results indicated that Western style diet such as expenditure of meat, milk and egg and bread had negative association with SMR. The expenditure of meat showed a negative association with SMR in all the three rice expenditure subgroups, but that of milk and egg in the low rice expenditure subgroup only. Consumption of constituents of the traditional Japanese diet, such as rice and other cereals, certain seasonings, salted and dried fish showed a positive association with SMR. Alcoholic beverages and cakes also showed a positive association. The secular trend of declining stroke mortality was parallel to the increase in the consumption of milk, meat, fish, eggs, soybeans, oil and fats and to the decrease in rice consumption. These findings were in accordance with the generally held view in Japan that moderate intake of animal protein was protective against stroke.

Cerebrovascular Disorders↗

Effects of dilazep on fibrinolytic system in animals. I. Enhancement of fibrinolytic activity by administered dilazep in ex vivo experiment.

The effects of dilazep on blood fibrinolytic system were studied in ex vivo experiment. Fibrinolytic activity in plasma was determined by the fibrin plate method at designed times after the administration of dilazep in guinea pigs, rabbits and mongrel dogs. In guinea pigs, fibrinolytic activity of euglobulin (plasminogen activator level, 1.10+/-0.08 CU/ml in control animals) rose to 1.53+/-0.24, 1.92+/-0.13 and 2.35+/-0.15 CU/ml 2 h after the p.o. administration of 30, 100 and 300 mg/kg of dilazep, respectively. Plasminogen levels in euglobulin were unchanged, however, plasmin inhibitory activities were lowered following the increase of plasminogen activator levels. About 2-fold increase of plasminogen activator level was also observed in rabbits 60 min after the p.o. administration of 300 mg/kg dilazep and returned to the initial level (0.054 CU/ml) within 3 h after the administration. A rapid increase of plasminogen activator level was observed in a case of the i.v. administration of dilazep into rabbits and mongrel dogs. In in vitro experiment, dilazep added to whole blood or plasma of guinea pigs ranging 0.1 to 1000 microgram/ml did not reveal any effects on plasminogen activator level or plasmin inhibitory activity in plasma.

Animals↗

[Anti-inflammatory effects of acemetacin (author's transl)].

It has been previously reported that acemetacin (ACM), the carboxymethyl ester of indomethacin (IND), is almost completely metabolized to IND in vivo. In the present study, the anti-inflammatory effects of ACM were compared with those of IND. ACM showed inhibitory effects on vascular permeability induced by phenylquinone. ACM inhibited carrageenin, kaolin, and nystatin edema dose-dependently and the ED30 in the carrageenin edema test was 2.8 Mg/kg. Inhibitory effects of ACM on carrageenin edema was not influenced by adrenalectomy or repeated administration of ACM. ACM also inhibited ultraviolet erythema at 5 mg/kg. Furthermore, ACM significantly inhibited granuloma formation at 2.5 mg/kg/day. The ED30 for the therapeutic effect on established adjuvant arthritis was 0.33 mg/kg/day. These anti-inflammatory effects of orally administered ACM were equivalent to or somewhat weaker than those of IND at an equimolar dose. Platelet aggregation was inhibited when ACM was given orally to rats and the activity of ACM was similar to that of IND. On the other hand, the effects of locally administered ACM on carrageenin and kaolin edema were markedly less potent than those of IND. In addition, inhibitory effects on prostaglandin synthesis and platelet aggregation in vitro were significantly weaker than those of IND. These results that the observed anti-inflammatory effects orally administered ACM are not due to its inherent action, but due to its metabolite, IND.

Animals↗

[Experimental studies of the effect of diltiazem (Ca-antagonist) on cerebral circulation and cerebral arterial spasm].

Polygraphycal studies were carried out in order to evaluate the effects of Diltiazem (Ca-antagonist) on cerebral circulation in cats. In addition to these studies, the inhibitory effect of Diltiazem on incubated blood-, and serotonin-induced vasocontraction of basilar arteries was observed through on operating microscope. Experimental vasospasm were induced by local administration of incubated blood and 10(-5)--10(-6) M serotonin solution. The required dose of Diltiazem, 30-300 micrograms/kg, was injected by one shot intravenously. CBF slightly decreased concomitantly with transiently decreased blood pressure shortly after intravenous injection of Diltiazem. After that, CBF increased for 15-20 minutes and returned to the state similar to that before the administration of Diltiazem. The changes in reactive pattern of CBF depend on the doses of Diltiazem and related to changes of CAR and VAR which were thought to indicate the blood of cardiac output. Vasocontraction induced by incubated blood and serotonin was released 30-35 per cent for 30 minutes after intravenous injection of Diltiazem 100 micrograms/kg. However, by administration of Diltiazem before and after local application of incubated blood and serotonin on basilar arteries, vasocontraction was inhibited completely. These data suggest that administration of Diltiazem (Ca-antagonist) might be useful in the prophylaxis and treatment of vasospasm following rupture of cerebral aneurysm.

Animals↗

[Cervical carotid endarterectomy for vertebrobasilar insufficiency].

A 66-year-old man was admitted with 2-year history of progressing gait difficulty and speech disturbance. His neurological examination on admission demonstrated dysarthria, truncal ataxia, mild foot weakness and emotional incontinence. Computerized tomography revealed enlarged ventricle with marked brain stem atrophy. Cerebral angiography showed 90% stenosis of left upper vertebral artery, 90% stenosis of mid basilar artery, occlusion of right upper vertebral artery and 50% stenosis with ulcer of right cervical carotid artery. Right cervical carotid endarterectomy was carried out with continuous electroencephalography (EEG) monitoring. Post-operative course was uneventful and the patient had gradual neurological improvement. This case was reported to be of help to judge the indication of such operation in effectiveness and also the value of EEG monitoring during operation.

Aged↗

[The association of cerebral aneurysm with malignant brain tumor--report of a case (author's transl)].

A rare case was reported in which a brain tumor was associated with a cerebral aneurysm. A 59-year-old woman was admitted with right spastic hemiparesis and motor aphasia. She was in somnolence and complained of headache. She had two attacks of loss of consciousness about 12 years previously. Since then she had right spastic hemiparesis and motor aphasia as sequelae. Preoperative CT scan and carotid angiograms showed a malignant brain tumor in the left temporal region and an anterior communicating artery aneurysm. Operation was performed in one stage. At first the neck of the aneurysm was clipped by right sphenoidal approach and the tumor was partialy removed by left temporal approach. The histological diagnosis was glioblastoma multiforme. The cerebral aneurysm associated with brain tumor should be treated as soon as possible. The operative result in our case was good.

Brain Neoplasms↗

High-performance liquid chromatographic assay of rat-brain dopamine and norepinephrine.

A high-performance liquid chromatographic method for the assay of dopamine (DM) and norepinephrine (NE) in rat brain has been developed. 3,4-Dihydroxybenzylamine was added to the sample as an internal standard. The isolated amines and the internal standard were subjected to reaction with fluorescamine, separated liquid chromatographically on a glycol-type gel and determined fluorimetrically. The standard deviation of the method was +/-2.7% for DM and +/-5.3% for NE. The contents of DM and NE in rat brain, with or without administration of a tyrosine hydroxylase inhibitor, as measured by the proposed method were always lower than those obtained by the trihydroxyindole method.

Animals↗