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Biomedical subjects

M Terashima

Publications and source records attributed to M Terashima.

At least 217 records · Page 12Linked to original sources
PubMed1988

[Application of the immunohistochemical method as predictive assay in radiotherapy of squamous cell carcinoma].

We analysed various subsets of lymphocytes infiltrated into cancer tissues from 15 patients (10 cases of oropharyngeal cancer and 5 of hypopharyngeal cancer) at pretreatment and at about 10 Gy and 30 Gy of irradiation by the method of biotin-avidin-horseradish peroxidase using mouse monoclonal antibodies. In 3 cases, tumor infiltrating lymphocytes were remarkably increased at the delivery of small dose of irradiation: Two of them showed remarkable radiosensitivity, and the lymphocyte subpopulation of the cancer tissue was mainly composed of Leu3a + 3b positive, Leu-8 negative and Leu-HLA-DR positive lymphocytes (activated helper inducer T lymphocytes). On the other hand, the third one proved to be rather radioresistant and the lymphocyte subpopulation was mainly composed of Leu-14 positive lymphocytes (B lymphocytes). These findings indicate that the analysis of the lymphocyte subpopulation infiltrating into cancer tissues at the delivery of small dose of irradiation is applicable as a predictive assay in radiotherapy of squamous cell carcinoma of oropharynx and hypopharynx.

Adult↗
PubMed1988

[Experimental study of the 6-day subrenal capsule assay by cyclophosphamide pretreatment].

For the purpose to suppress the host immune reaction in subrenal capsule assay (SRCA) using normal immunocompetent mice, the effects of cyclophosphamide (CPM) pre-treatment (CPM 180 mg/kg day-1 s.c.) were compared with cyclosporine A (CSA) treatment (CSA 60 mg/kg s.c. daily). CPM and CSA suppressed host reaction until day 6 and day 12, respectively. However, in the histological evaluation of day 6 tumor, there was no difference between two groups. Mouse serum levels of CPM were measured by a bioassay as indicated by the cytotoxicity against P388 cells, to evaluate its residual activity against implanted tumor. No cytotoxicity was observed in the serum of 36 hr. after CPM injection. The antitumor activities of several chemotherapeutic agents against serially transplanted human esophageal cancer xenograft (IMEs-1) in untreated control group and CPM pre-treated group of SRCA were compared with the subcutaneous transplantation assay in nude mice. In all agents, the drug activities in CPM pre-treated group were similar to that in nude mice assay system. The increase of toxicity in CPM pre-treated group was very light. The immunosuppressive activity of CPM were also tested in 14 clinical samples. CPM suppressed the host reaction satisfactorily in all samples. These results suggest the usefulness of CPM pretreatment for suppressing the host reaction in SRCA.

Animals↗
PubMed1988

[Clinical significance of serum levels of SCC antigen in patients with esophageal squamous cell carcinoma].

The levels of squamous cell carcinoma-related antigen (SCC) in sera of 71 patients with esophageal squamous cell carcinoma, 7 patients with benign esophageal diseases, 11 gastric cancer patients and 15 normal volunteers were studied in order to evaluate its clinical significance as a tumor marker. In the patients with esophageal carcinoma, immunosuppressive acidic protein (IAP) and carcinoembryonic antigen (CEA) were also measured simultaneously. In the normal volunteers, patients with benign esophageal diseases and gastric cancer patients, the SCC levels were negative except for only one patient. However, in patients with carcinoma of the esophagus 37 out of 71 were positive, the positivity rate being 52.1%. Comparison among SCC, IAP and CEA showed that the positivity rates for SCC and IAP increased with progression of the disease. In contrast, CEA levels did not correspond to clinical stage except in several patients with non-resectable and recurrent disease. With regard to the changes in serum levels of SCC, IAP and CEA before and after surgery and radio-chemotherapy. SCC was the most sensitive marker of the three, and responded well to the effects of therapy. SCC was thus considered to be a useful marker for monitoring esophageal cancer patients.

Aged↗
PubMed1987

Production of human--human hybridomas secreting antibody to sheep erythrocytes after in vitro immunization of peripheral blood lymphocytes.

This report describes the formation of human hybridomas after in vitro immunization of peripheral blood lymphocytes (PBL) with an antigen and fusion of the stimulated lymphocytes with a HAT-sensitive human myeloma cell line, RPMI 8226. PBL were stimulated in vitro with sheep erythrocytes (SRBC) plus fresh human serum. PBL of some donors produced anti-SRBC antibody when they were cultured at 2 X 10(6) cells per well in a 24-well plate with the antigen plus fresh human serum for 7 days. Although lymphocytes of some donors were "low-responders" under the above conditions, they responded to SRBC when they were cultured with not only the antigen plus fresh human serum but also with the culture supernatant obtained after phytohemagglutinin (PHA) stimulation of a mixture of PBL from two donors (MLC-PHA sup). The cells sensitized by this procedure were fused with RPMI 8226 cells. Hybrids secreting IgM or IgG anti-SRBC antibodies were obtained. Additionally the ratio of total IgG-producing hybridomas to IgM-producing ones was higher when the MLC-PHA sup was used at the time of the in vitro immunization.

Animals↗
PubMed1987

[Analysis of the chemosensitivity test by subrenal capsule assay].

The antitumor activity of several chemotherapeutic agents against a total of 30 clinical samples obtained from 30 human tumors from the esophagus (12), stomach (10), colorectum (6) and lung (2), were tested by subrenal capsule assay (SRCA) using normal immuno-competent BDF, mice. The antitumor activity was evaluated by changes in both tumor size (delta TS method) and tumor growth inhibition rate (TGIR method). Among the 30 tumors, 15 were also tested by human tumor clonogenic assay (HTCA) and the results obtained in the two assays were compared. In the SRCA, adequate growth of the tumor in the control group for evaluation of the response of the treated group was obtained from 27 out of 30 tumors (90%). With activity criteria set at delta TS less than or equal to -1.0 dmm and TGIR greater than or equal to 50%, 41% of the drugs tested were active in delta TS while 27% were active in TGIR. When relationships between antitumor activities evaluated by delta TS and TGIR was compared, both activities were well correlated (r = -0.64). Correlations between tumor responses in the HTCA and in the SRCA were tested in 6 tumors treated with 20 drugs. The overall accuracy was 60% by the TGIR method and 70% by the delta TS method, respectively.

Animals↗
PubMed1987

[Analysis of the chemosensitivity test by human tumor clonogenic assay and subrenal capsule assay].

The antitumor activities of several chemotherapeutic agents were tested in the human tumor clonogenic assay (HTCA) and in subrenal capsule assay (SRCA). The results obtained in these two assays and clinical response were compared. 1. Colony growth was observed in 43 of 73 tumors (58.9%), and the adequate colony growth to evaluate the response of therapeutic groups was obtained in 21 tumors (28.8%). 2. Control growth adequate to meet evaluable assay criteria was obtained in 38 of 43 tumors (88.4%). 3. With activity criteria set at a decrease of greater than or equal to 50% in tumor colony-forming units for the HTCA and a change in tumor size less than or equal to -1.0 dmm for the SRCA, 24 of 98 drugs tested were active in HTCA (24.5%), and 50 of 160 drugs were active in SRCA (31.2%). 4. A total of 21 tumors was tested in both HTCA and SRCA and the tumor response was compared in 12 tumors treated with 39 drugs. Correlation of tumor responses between the two assays was 71.8%. 5. A total of 8 HTCA-clinical correlations and 5 SRCA-clinical correlations were possible. Overall predictive accuracy was 87.5% in the HTCA and 80.0% in the SRCA, respectively.

Antineoplastic Agents↗
PubMed1986

Induction of in vitro antigen-specific antibody production against NIP-KLH in nonadherent cells derived from human peripheral blood mononuclear cells.

A primary in vitro antibody response to 4-hydroxy-3-iodo-5-nitrophenylacetyl keyhole limpet hemocyanin (NIP-KLH) by plastic dish nonadherent human peripheral blood mononuclear cells (PBMC) was examined. Nonadherent cells of most donors produced an anti-NIP antibody when they were cultured with the antigen at a cell density of 2.5 X 10(6)/ml in the presence of Staphylococcus aureus Cowan I (SAC) at a concentration of 0.003% for 5 days, washed and further cultured in the absence of the antigen and SAC for an additional 5 or 6 days. Nonadherent cells of 'low-responders' responded to NIP-KLH plus SAC and produced anti-NIP antibody when they were cultured with not only NIP-KLH plus SAC but also with the culture supernatant obtained on phytohemagglutinin (PHA) stimulation of a mixture of PBMC from 2 donors. Furthermore, it was found that the PHA supernatants could be very effectively substituted for by human recombinant interleukin-2.

Antibody Formation↗
PubMed1986

A long-term follow-up study on the efficacy of further attenuated live measles vaccine, Biken CAM vaccine.

Antibody persistence was measured in 39 children in an open community 12-13 years after immunization against measles with further attenuated live vaccine, Biken CAM. Serum samples of the children taken every two or three years after vaccination had higher, lower, or the same HI antibody titers as those in samples taken 6 weeks after vaccination. These differences reflected a decrease in the titer in some children and subclinical natural reinfection in others. However, all the children still retained detectable antibody in 12 or 13 years after vaccination, indicating long-term persistence of immunity after immunization with Biken CAM vaccine. For evaluation of the protective efficacy of the vaccine, matched controls were studied during the same period. Serological examination revealed that 97.5% of the controls were infected with measles and contracted the disease. In contrast, none of the vaccinees developed clinical infection after close contact with measles patients.

Adolescent↗
PubMed1985

Ultrasonic aid in transvenous instrumental dilatation for patients with membranous stenosis of the hepatic portion of the inferior vena cava.

We report two cases of a membranous stenosis of the hepatic portion of the inferior vena cava. Membranotomy was performed with a specially designed instrument similar to a Tubbs' dilator following balloon membranotomy. During the procedures, ultrasonographic observations were made and this technique was confirmed to be effective for proper placing of a balloon and a dilator in the stenotic portion. Follow-up evaluation of the procedures was also feasible with this ultrasonic aid.

Angioplasty, Balloon↗
PubMed1985

Isolation and properties of two phospholipases A2 from the venom of an Australian elapid snake (Pseudechis australis).

Two phospholipases A2 (Pa-11 and Pa-13) were purified from the venom of an Australian elapid snake (subfamily Acanthophiinae) Pseudechis australis (king brown snake) by chromatography on CM-cellulose CM-52 followed by gel filtration on a Sephadex G-75 column. The apparent molecular weights of the two phospholipases A2 (Pa-11 and Pa-13) were 14,000 and 13,500, respectively, by gel filtration analysis on a Sephadex G-75 column. Each enzyme molecule consists of a single polypeptide chain of 118 amino acid residues. The isoelectric points of Pa-11 and Pa-13 were 10.5 and 10.0, respectively. The optimum pH values of Pa-11 and Pa-13 for hydrolysis of egg-yolk phosphatidylcholine were 7.8 and 7.5, respectively. Pa-11 was lethal to mice (LD50 0.23 micrograms/g body weight), whereas Pa-13 showed no lethal activity at a dose level of 7.4 micrograms/g mouse. Each enzyme was inactivated by reaction with p-bromophenacylbromide on the sole histidine residue (Pa-11) or on one of the two histidine residues (Pa-13). Oxidation of the tryptophan residues in Pa-11 and Pa-13 with N-bromosuccinimide led to a decrease in the phospholipase A activity. A complete loss of both enzymic and lethal activities of Pa-11 was observed upon oxidation of one of the two tryptophan residues of the molecule.

Acetophenones↗
PubMed1985

Amino acid sequences of phospholipases A2 from the venom of an Australian elapid snake (king brown snake, Pseudechis australis).

Two basic phospholipases A2 (Pa-11 and Pa-13) have been isolated from the venom of an Australian elapid snake, Pseudechis australis (king brown snake). The reduced and S-carboxymethylated phospholipases A2 were digested with trypsin and the resulting peptides were purified by a combination of chromatography on a DEAE-cellulose DE-52 column and gel filtration procedures. Eleven main peptides from Pa-11 and 9 peptides from Pa-13 could account for the amino acid compositions of the respective enzyme molecules. The alignment of the tryptic peptides and unelucidated regions of the amino acid sequences of tryptic peptides were established by the analysis of the peptides obtained by chymotryptic and/or Staphylococcal protease digestions. Each phospholipase A2 consisted of a single chain of 118 amino acid residues, including 14 half-cystine residues. Although Pa-11 is enzymatically 30-times as active as Pa-13 and highly toxic as compared to Pa-13, they are highly homologous in their amino acid sequences. They are also homologous to the enzymes from mammalian pancreas and the other snake venom phospholipases A2, especially to those from snakes belonging to the subfamilies Acanthophiinae and Laticaudinae.

Amides↗
PubMed1985

A hapten-induced conformational change accompanies the cryoprecipitation of an immunoglobulin.

By simultaneous spectroscopic measurements (absorbance, light scattering, fluorescence and circular dichroism) the temp-dependent behavior of a cryoglobulin, the mouse monoclonal immunoglobulin G to N-acetyllactosamine, has been observed. The results show that a hapten-induced conformational change in the structure of a cryoglobulin can accompany its intermolecular association. This association of the immunoglobulin increases after hapten saturation of its two antigen-combining sites.

Amino Sugars↗
PubMed1985

[Enhanced cytotoxic effect of Adriamycin and vincristine against PC-6 (human lung cancer cell line) and Hattori (human breast cancer cell line)].

Enhancements of cytotoxic effects of adriamycin (ADM) and vincristine (VCR) on PC-6 (lung cancer cell line) and Hattori (breast cancer cell line) were investigated by concomitant use of a calmodulin inhibitor: nicardipine, antiplatelet agents: oxyfedrine and trimethazidine and an antihypertensive agent: trichlormethiazide. Nicardipine increased the cytotoxic activity of both drugs but other agents did not produce any significant increase. Nicardipine increased the cytocidal effect of VCR on PC-6 about 2-fold and on Hattori about 3-fold. Furthermore, it also increased the effect of ADM on Hattori about 2-fold. Thus, nicardipine enhanced the cytocidal activity of VCR more remarkably than that of ADM.

Antineoplastic Combined Chemotherapy Protocols↗