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Biomedical subjects

M Takao

Publications and source records attributed to M Takao.

At least 163 records · Page 9Linked to original sources
PubMed1987

Demonstration of rod and cone photoreceptors in the lamprey retina by freeze-replication and immunofluorescence.

In common with other cyclostomata, the Japanese river lamprey (Lampetra japonica) has a retina consisting of distinct types of photoreceptor cells called long and short photoreceptor cells. After freeze-fracture, disc membranes of these photoreceptor cells were characterized in common by a homogeneous distribution of intramembrane particles on the protoplasmic fracture faces, in contrast to those of the myeloid bodies bearing scattering particles. Immunofluorescent examination was applied to the retina with monoclonal antibodies raised against bovine and chicken rhodopsins. Positive immunoreactivity was found to be limited to outer segments of the short cell, leaving the entire body of the long cell and all other components of the retina negative. The results suggest that the short cell is more closely related to a rod-type photoreceptor cell characterized by rhodopsin as its visual pigment.

Animals↗
PubMed1987

Cloning and characterization of the 5' region of the cell wall protein gene operon in Bacillus brevis 47.

Bacillus brevis 47 secretes vast amounts of proteins derived from both middle wall protein (MWP) and outer wall protein into the medium. The 5' region of the cell wall protein gene operon was cloned into Bacillus subtilis and subsequently into B. brevis 47. On the basis of the nucleotide sequence analysis, an open reading frame coding for MWP was identified on the cloned DNA fragment. Two potential translation initiation sites for the MWP gene are located tandemly in the same reading frame. Each of the sites contains a sequence highly homologous to the 3' end of B. brevis rRNA and an initiation codon. The translational fusion of the 5' region of the MWP gene with the Bacillus licheniformis alpha-amylase gene resulted in the efficient expression of the alpha-amylase gene in B. brevis 47. Of the two potential translation initiation sites, the one located upstream could be eliminated without affecting the expression of the MWP-alpha-amylase fusion gene, suggesting that MWP is synthesized in a precursor form with a signal peptide of 23 amino acid residues. S1 nuclease mapping of the cell wall protein gene transcripts suggested the possibility of the existence of several promoters in the 5' region within 300 base pairs from the translation initiation sites; one promoter was definitely localized within this part of the 5' region, and it was capable of expressing a heterologous gene fusion at a high level. The roles of the apparent structural complexity of the 5' region of the cell wall protein gene operon are discussed in connection with the efficient gene expression.

Amino Acid Sequence↗
PubMed1987

Ultrasonic flowmetry and neodymium-yttrium aluminum garnet laser for nephrolithotomy.

Intraoperative Doppler flowmetry was used for localization of the intrarenal arteries. To reduce blood loss during nephrolithotomy, a neodymium-yttrium aluminum garnet (YAG) laser beam was used clinically as a cutting instrument. The combination of intraoperative localization of intrarenal arteries by Doppler flowmetry and cutting through renal parenchyma using the neodymium-YAG laser beam was used in 7 patients with stag-horn calculi or recurrent stones. The average blood loss was only 185 ml. Postoperative renal function was normal. Intravenous pyelograms revealed prompt function and no evidence of contrast extravasation. The data indicate that this technique is effective, diminishes blood loss, and does not impair renal function.

Humans↗
PubMed1987

[Endoscopic ethanol injection treatment for bladder cancer].

One invasive and 4 superficial bladder cancers were treated by local injection of absolute ethanol through an endoscope. With the patient placed in the lithotomy position, an endoscope was introduced after mucosal anesthesia with xylocaine jelly. According to the usual manner of retrograde catheterization, a 23G syringe needle attached to a 5F ureteral catheter was advanced into the base of the tumor and through the needle absolute ethanol was injected. This treatment is characterized by endoscopic delivery of ethanol as with sclerotherapy for esophageal varices to obtain a potent necrotizing effect as achieved by transcatheter embolization of ethanol for the treatment of renal cell carcinoma. As a rule, this technique does not require any anesthesia other than mucosal anesthesia with xylocaine jelly and can be done repeatedly and very easily, thus having a high cost effectiveness. This treatment serves as a good palliative therapy for invasive bladder cancers, because of its rapid achievement of hemostasis and reduction of the tumor masses. Furthermore, this treatment is just as effective as standard transurethral resections to eradicate superficial bladder cancer, even though the treatment may have to be repeated.

Administration, Intravesical↗
PubMed1987

[A case of Cushing's syndrome caused by an adrenal black adenoma].

A black adenoma of the adrenal cortex was surgically removed from an 18-year-old female who had clinical and laboratory findings characteristic of Cushing's syndrome. The electron microscopic study revealed typical features of steroid-producing cells, including a prominent smooth and rough endoplasmic reticulum, and mitochondria. In addition, many of the adenoma cells contained numerous pigmented granules which were lipofuscin. Three months after the surgery, the hormone levels returned to the normal range. The Japanese literature of functional black adenoma is reviewed.

Adenoma↗
PubMed1987

[A case of bilateral synchronous renal cell carcinoma managed with radical nephrectomy and ex-vivo tumor enucleation].

A 61-year-old female presented with gross hematuria and left flank pain. The arteriogram and CT scan revealed a large left renal tumor and multiple small right renal metastatic tumors. A chest X-ray and Ga scintigram showed no other metastatic lesions. A left radical nephrectomy and a right renal bench surgery were performed at the same time. The right multiple tumors were enucleated and the right kidney was autotransplanted into the right iliac fossa. The postoperative course was uneventful and the patient was free of tumor with good renal function. Renal preservation surgery was considered more beneficial for this patient than bilateral nephrectomy followed by hemodialysis.

Carcinoma, Renal Cell↗
PubMed1985

Genetic Rearrangement of Plasmids: In Vivo Recombination between a Dehalogenation Plasmid and Multiple-Resistance Plasmid RP4 in Pseudomonas sp.

When Moraxella plasmid pUO1 encoding haloacetate dehalogenase and mercury resistance coexisted with IncP-1 plasmid RP4 in Pseudomonas sp., genetic exchange between the plasmids often occurred, probably by site-specific recombination. The recombinant plasmids obtained were classified into four groups on the basis of phenotype. Representative plasmids for each group were analyzed for DNA composition and function, and the mechanism for the formation of these plasmids was sought. They were inherited stably in Escherichia coli and a Pseudomonas sp.

Journal Article↗
PubMed1985

[Concentration level of latamoxef sodium (LMOX) in prostatic tissue].

The concentration of latamoxef sodium (LMOX) in serum and prostatic tissue was evaluated. Thirty seven patients with benign prostatic hypertrophy were given 1 g LMOX intravenously prior to prostatectomy. Prostatic tissue and blood were sampled at 1, 2 or 3 hrs after administration of LMOX. The concentration of LMOX in prostatic tissue was 17.4 +/- 3.4 micrograms/g tissue, 11.1 +/- 1.3 microgram/g tissue and 8.9 +/- 1.3 microgram/g tissue 1, 2 and 3 hrs after injection, respectively (mean +/- S.E.). The penetration ratio of LMOX (concentration in tissue/in serum) to prostate was 30-45%. Therefore, the 80% MIC of LMOX is lower than 8 micrograms/ml for most gram negative bacteria, the results suggest that LMOX is very effective against prostatitis caused by these organisms.

Aged↗
PubMed1985

Functional visual field of patients with visual field loss.

To assess the capability of perceiving forms in patients with visual field loss, a concept of functional visual field was introduced based on determinations of the time required for pattern recognition. Two series of stimulus patterns were made of Japanese syllabic hiragana characters drawn with black dots in the background of open circles of various sizes: the clear stimulus had only open circles in the background and the noisy stimulus had black dots scattered in the background. The stimuli were presented for various durations to 15 normal subjects and 25 patients with narrowed visual field; a correlation of the percentages of correct pattern recognition with the stimulus durations permitted calculations of the 50% recognition time. The recognition time was longer with the noisy than with the clear stimuli. The recognition time with a given stimulus size was longer in patients than in normal subjects. In 3 normal subjects the visual field was artificially narrowed and the recognition time was determined. The recognition time could be expressed by a power function of the ratio of the effective visual field diameter to the diameter of the stimulus pattern. On this basis the functional visual field size of a patient was defined as the size of the artificially narrowed visual field of the normal subject that required the same recognition time as that of the patient. The functional visual field of patients could be correlated with the area of the perimetric field with the V/4 target of Goldmann's projection perimeter. The concept of the functional visual field was found to be useful to express the patients' capability for pattern perception.

Adult↗