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Biomedical subjects

M Takada

Publications and source records attributed to M Takada.

At least 667 records · Page 37Linked to original sources
PubMed1984

[Local application of anti-cancer drugs for the treatment of malignant pleural and pericardial effusion].

Pleural effusion is a common complication in patients with malignant neoplasm. A randomized controlled study of intrapleural instillation of Adriamycin (control group, 30 patients) and Adriamycin Nocardia rubra cell wall skeleton (N-CWS group, 26 patients) with tube thoracostomy was performed in 55 patients with malignant pleural effusion due to primary lung cancer. The response rates for control of pleural effusion were 73.4% in the N-CWS group and 46.1% in the N-CWS group. These results suggest that intrapleural instillation using a combination of anti-cancer agent and immunopotentiator is an effective treatment for malignant pleurisy. Cardiac tamponade secondary to cancer is a life-threatening complication requiring immediate treatment. Twenty-four patients with malignant pericardial effusion were treated by intrapericardial instillation of anti-cancer drugs, such as Carbazilquinone, Mitomycin-C or ACNU, with pericardial drainage. The range of survival time from the instillation of anti-cancer drug was 3-365 days (average days). In only 4 patients, reaccumulation of pericardial effusion was recognized. There were no serious complications with this procedure. It was considered that local instillation of anti-cancer agents with pericardial drainage was a useful therapeutic modality for malignant pericarditis.

Adenocarcinoma↗
PubMed1984

[Intraperitoneal transplantation derived from spontaneous colonic cancer of WF rat strain].

Intraperitoneal transplantation of spontaneous colon carcinoma of WF strain has been established. There are two lines of transplantable colon carcinoma derived from the male and female rat of the same litter (MM4-48H). These two lines are now (Sept. '83) at the 16th generation. They have been succeeded every 3 weeks intraperitoneally using 4-weeks old male and female siblings of WF strain. Histology of these transplantable colon carcinoma was well-differentiated tubular adenocarcinoma at the first few generations but changed gradually medullary tubular pattern at the 16th generation. We believe this is the first report of transplantable tumor lines from the spontaneous colon carcinoma itself and these lines can be used for screening test of carcinostatics.

Adenocarcinoma↗
PubMed1983

The posteromedial ventral nucleus of the thalamus (VPM) of the cat: direct ascending projections to the cytoarchitectonic subdivisions.

The posteromedial ventral nucleus (VPM) of the cat is divided cytoarchitectonically into the magnocellular (VPMmc), lateral parvocellular (VPMpcl), and medial parvocellular (VPMpcm) divisions. Cell bodies of neurons in the VPMpcm are small, while those in the VPMpcl are small to medium-sized. The VPMmc contains large neurons. Direct projections from the lower brain stem structures to each of the three divisions of the VPM were examined by the retrograde horseradish peroxidase (HRP) method. When HRP injection was done into the VPMmc, labeled neurons were mainly located contralaterally in the ventral division of the principal sensory trigeminal nucleus (Vp), in the rostral part of the oral subnucleus in the spinal trigeminal nucleus (Vsp), and in the interpolar subnucleus of the Vsp; a few labeled neurons were also found contralaterally in lamina I of the caudal subnucleus of the Vsp. When HRP injection was restricted to the VPMpcl or VPMpcm, HRP-labeled neurons were mainly observed ipsilaterally, respectively, in the dorsal division of the Vp, or in the parabrachial nucleus (PBN) regions dorsomedial and ventromedial to the brachium conjunctivum. After HRP injection into the parvocellular part of the VPM (VPMpc), labeled neurons were also seen contralaterally in the Vsp, but these were far less numerous than those seen after HRP injections into the VPMmc. Thus, each of the three divisions of the VPM receives main ascending afferent fibers from different brain stem structures; the VPMpcm, VPMpcl, or VPMmc receives afferent fibers, respectively, from the PBN ipsilaterally, from the dorsal division of Vp ipsilaterally, or from the ventral division of the Vp and the Vsp contralaterally.

Afferent Pathways↗
PubMed1983

Transport of [14C]coenzyme Q10 from the liver to other tissues after intravenous administration to guinea pigs.

After intravenous injection of [14C]coenzyme Q10 into guinea pigs, the blood level of radioactivity declined rapidly within 30 min. However, radioactivity in the blood subsequently increased, reached a peak at 8 h after the injection and thereafter decreased slowly. Since the liver uptake of radioactivity at 30 min after the injection was 87.8% of the dose and the increase of blood level was also seen in guinea pigs with bile duct fistula, it was concluded that the radioactivity taken up by the liver was redistributed to the blood and then transferred to other tissues. Tissue radioactivity levels were highest in the liver and spleen at 30 min after the injection and decreased thereafter. The levels in the blood and kidney peaked at 8 h, while those in the heart and brain peaked at 24 h and subsequently decreased. In contrast, the level in the adrenal gland was still increasing at 168 h after the injection; it rose sharply from 30 min, and at 24 h was higher than the level in any other tissue. Electrophoretic analysis showed that the radioactivity in serum at 8 h after the injection was associated with lipoproteins. It was confirmed by thin layer radiochromatography that the radioactivities in serum and liver at various times were due to coenzyme Q10 and coenzyme Q10 hydroquinone.

Animals↗
PubMed1983

A pretectofacial projection in the cat: a possible link in the visually-triggered blink reflex pathways.

A direct projection from the pretectum to the facial motor nucleus was shown to exist in the cat by the anterograde and retrograde horseradish peroxidase (HRP) methods. Pretectofacial fibers arise from the olivary pretectal nucleus and end mainly in the dorsal division of the facial motor nucleus, bilaterally, with a contralateral predominance. It is known that the olivary pretectal nucleus receives retinal fibers, and that the dorsal division of the facial motor nucleus contains orbicularis oculi motoneurons. Thus, the pretectofacial fibers are assumed to cause protective lid closure with certain visual stimuli.

Animals↗
PubMed1983

Direct projections from the anterior pretectal nucleus to the dorsal accessory olive in the cat: an anterograde and retrograde WGA-HRP study.

Direct projections from the anterior pretectal nucleus (APN) to the dorsal accessory olive (DAO) were found in the cat by the anterograde and retrograde WGA-HRP methods. The dorsal or the ventral portions of the rostral half of the APN pars compacta send fibers respectively to the lateral or the medial portions of the whole rostrocaudal extent of the DAO. These APN-DAO fibers can be considered to play roles in some somatomotor mechanisms.

Animals↗
PubMed1983

Triphenyltin fluoride in vitro inhibition of rabbit platelet collagen-induced aggregation and ATP secretion and blockade of arachidonic acid mobilization from membrane phospholipids.

Recent studies have demonstrated that triphenyltin fluoride (TPTF) inhibits collagen-induced aggregation and ATP secretion of rabbit platelets in vivo [S. Manabe and O. Wada, J. Toxic. Sci. 6, 236 (1981)]. The aim of the present investigation was to test the effects in vitro of TPTF on platelet aggregation and to elucidate the mechanism of the inhibitory action by studying the release and metabolism of arachidonic acid and the cyclic AMP contents of rabbit platelets treated in vitro with TPTF. Although no inhibitory effect of TPTF was found on sodium arachidonate-induced platelet aggregation and ATP secretion, TPTF inhibited both reactions induced by collagen. Triphenylarsine and triphenylantimony did not inhibit, even at a concentration of 10(-3) M. The anti-aggregating concentration (IC50) of TPTF was 6.0 x 10(-6) M against collagen. TPTF had no inhibitory effect on the conversion of exogenous arachidonic acid to malondialdehyde (MDA) by platelets, while the collagen-induced production of arachidonate metabolites [MDA, 12-L-hydroxy-5,8,10-heptadecatrienoic acid (HHT) and thromboxane B2] was remarkably inhibited by TPTF. Furthermore, TPTF apparently inhibited the collagen-induced release of arachidonic acid from platelets, although the formation of phosphatidic acid was not inhibited. Total cyclic AMP content after TPTF exposure was not changed significantly. These results indicate that TPTF inhibited the collagen-induced arachidonic acid release from platelet phospholipids, presumably by acting on phospholipase A2. Furthermore, it seems unlikely that the inhibition of arachidonic acid release by TPTF can be explained by the level of cyclic AMP in platelets.

Adenosine Triphosphate↗
PubMed1983

A light and electron microscopic study of premotor neurons for the trigeminal motor nucleus.

Premotor neurons sending their axons to the trigeminal motor nucleus were observed in the cat by light and electron microscopy after labeling the neurons retrogradely or anterogradely with horseradish peroxidase (HRP). After HRP injection into the trigeminal motor nucleus, retrogradely labeled neurons were seen most frequently in the parvocellular reticular formation bilaterally. Many labeled neurons were also seen contralaterally in the intermediate zone at the rostralmost levels of the cervical cord and its rostral extension into the caudalmost levels of the medulla oblongata. Additionally, some neurons were labeled ipsilaterally in the mesencephalic trigeminal nucleus, contralaterally in the main sensory trigeminal nucleus and the trigeminal motor nucleus, and bilaterally in the oral and interpolar subnuclei of the spinal trigeminal nucleus. Only a few labeled neurons were seen in the confines of the gigantocellular reticular formation. All labeled neurons were small or of medium size; no large neurons were labeled. After HRP injection into the regions around the trigeminal motor nucleus or the parvocellular reticular formation, axodendritic terminals containing HRP granules were found contralaterally within the trigeminal motor nucleus. Some of these labeled terminals were filled with round synaptic vesicles and others contained pleomorphic synaptic vesicles. The varied morphology of labeled axon terminals was considered to reflect the functional heterogeneity of the premotor neurons for the trigeminal motor nucleus.

Animals↗
PubMed1983

Postzygotic D/D translocation homozygosity associated with recurrent abortions.

A Robertsonian translocation involving homologous D chromosomes was found in two cases with a history of recurrent abortions. In the first case the propositus was a 37-year-old phenotypically normal man who had a balanced t(14q14q) translocation. In the second case, a 27-year-old phenotypically normal woman was found to be a balanced t(15q15q) translocation carrier. The recurrent abortions in both cases were probably owing to this translocation.

Abortion, Habitual↗
PubMed1983

The role of tissue thromboplastin in the development of DIC accompanying neoplastic diseases.

Procoagulant activity of gastric cancer tissues and leukocytes obtained from various types of leukemia have been studied with special reference to TTP. The following results were obtained. Homogenates of APL leukocytes and gastric cancer tissues contained strong procoagulant activities, most of which have been identified as TTP since the activities were neutralized by a specific antibody against purified human placenta TTP, inactivated by the removal of phospholipid with heptane-butanol mixture, and inactivated by the addition of phospholipase C. The delipidated homogenates regained procoagulant activities by relipidation procedures. These results also confirmed that TTP from APL leukocytes and gastric cancer tissues have the same lipoprotein properties as those of TTP in normal tissues. Though slight proteolytic activity and fibrinolytic activity were demonstrated in the homogenate of gastric cancer tissues, it was noted that the TTP activity was different from these two activities by partial purification of TTP from gastric cancer tissues. The TTP activity of 9 homogenates of gastric cancer tissues was 301 +/- 289 (mean +/- SD) units per mg protein, being higher in homogenates of mucinous adenocarcinoma and signet-ring cell carcinoma than in those of tubular and poorly differentiated adenocarcinoma. The mean TTP activity of leukocyte homogenates from 14 patients with APL and one out of 4 patients with CML in blastic crisis was 81 +/- 76 units/10(7) cells. The TTP activity of the homogenates of leukocytes from 7 out of 18 patients with AML and another patient with CML in blastic crisis ranged from one to six units/10(7) cells with a mean of 3.3 +/- 1.2. The TTP activity of leukocyte homogenates from the other 11 cases of AML, two cases of CML in blastic crisis, 6 cases of CML, and one case each of ALL and CLL were less than one unit/10(7) cells. In leukemic patients, all cases with a value of more than 202 for the product of units of TTP activity per 10(7) cells and differential count (%) of leukemic cells in the bone marrow smear (MU value) were accompanied by DIC. The MU value of leukemic patients correlated well to the plasma fibrinogen and serum FDP levels. All patients with a MU value of more than 277 died of DIC when a sufficient amount of heparin was not administered. On the other hand, no DIC developed in any of the patients with a MU value of less than 90.(ABSTRACT TRUNCATED AT 400 WORDS)

Blood Coagulation↗
PubMed1983

Criteria for diagnosis of DIC based on the analysis of clinical and laboratory findings in 345 DIC patients collected by the Research Committee on DIC in Japan.

From analysis of the clinical and laboratory findings in the DIC patients collected by JRDC, diagnostic criteria for the diagnosis of DIC are proposed. The characteristics of the criteria are as follows: (1) a scoring system is adopted; (2) as the tests for scoring, PT, plasma Fbg level, serum FDP level, and platelet count are used; (3) different scores are given according to the grade of change in the results of the tests; (4) bleeding and clinical signs indicating organ dysfunction due to DIC are included in the scoring system; and (5) recognition of the well-known etiological factors of DIC is also scored.

Adolescent↗