Search PubMed⌕ Search

Biomedical subjects

M Tajima

Publications and source records attributed to M Tajima.

At least 217 records · Page 12Linked to original sources
PubMed1988

[Serological survey of feline leukemia virus infection and the outcome of antibody-positive cats].

A serological survey was carried out to examine the presence of antibodies against feline leukemia virus (FeLV) and feline oncornavirus-associated cell membrane antigen (FOCMA) in 208 cat sera collected at Teikyo University School of Medicine. Seven cats (3.4%) were positive for FeLV antibodies by enzyme-linked immunosorbent assay whereas no cat was positive for FOCMA antibody by indirect membrane immunofluorescent test. Anemia, leukemia and/or lymphoma formation were not observed in these FeLV antibody-positive cats. But among these seven cats, three were positive for toxoplasma antibodies. One of them was also positive for Chlamydia psittaci antibody and it died in pneumonia. Among the four toxoplasma antibody negative cats, one was died in eosinophilic granuloma. Furthermore, two of three cats, which were used for experiments, had cold and took therapy.

Animals↗
PubMed1988

[Testicular measurement by test size orchidometer].

The testicular volume of 1782 testes in 891 subjects between 0 and 86 years old was measured using the Test Size (Resimed Co, Switzerland), a metrological equipment for testis. We also compared the testicular volume calculated from tomographic images by testicular ultrasonographic measurements in some of these subjects and the actual volume of the isolated testes from the same subjects. The testicular volume measured with Test Size gradually grew at the age of 0 to about 8 years, rapidly increased in percentage at the age of about 12 to 13 years and reached to a peak at about 18 to 19 years. Comparison between the actually measured volume of isolated testes and the testicular volume with Test Size resulted in an index of 0.608 while comparison between that and the ultrasonographically measured testicular volume resulted in an index of 1.098. Therefore, authentic testicular volume is calculable by means of this method.

Adolescent↗
PubMed1987

Sequence analysis of elastin cDNA from chick aorta and tissue-specific transcription of the elastin gene in developing chick embryo.

A portion of elastin cDNA with a size of 1.5 kilobase pairs (kb) was cloned from chick aorta. Sequence analysis revealed that the cDNA consists of 0.9 kb of coding region and 0.6 kb of 3'-untranslatable region. The primary structure of the peptide deduced from the coding sequence exhibited a strong homology with the published data from sheep and bovine elastin cDNA. The abundance of elastin mRNA in the aorta and skin was studied in developing chick embryo by Northern analysis using the cDNA as a probe. The elastin mRNA level in the aorta gradually decreased in the late half of development, while the elastin mRNA level in the skin was dramatically elevated between the 18th and 21st days. These results strongly suggest that the transcription of the elastin gene was controlled specifically in the respective organ during development.

Amino Acid Sequence↗
PubMed1987

Cytoplasmic crystalline inclusions in hepatocytes of a steer with shipping fever.

Needle-shaped crystalline inclusion bodies were found in vacuolated liver cells of a steer affected with pneumonic pasteurellosis. The inclusions were eosinophilic and refractive, and ranged in size from 3 to 6 microns long and from 150 to 250 nm wide. Histochemical examination revealed that they contained lipid and protein. Electron microscopic examination showed finely fibrillar or granular material. Cross-striations with a periodicity of 45 nm were seen in longitudinal section of the inclusions. The possible origin and pathogenic significance of the inclusions are discussed.

Animals↗
PubMed1987

Expression of the human adenovirus E1a product in yeast.

We synthesized the 13S mRNA-encoded protein of the early region 1a (E1a) of human adenovirus in Saccharomyces cerevisiae under the control of the yeast GAL7 gene promoter. Similar to the case in HeLa cells, the E1a protein in yeast was phosphorylated and formed multiple bands on sodium dodecylsulfate-polyacrylamide gel electrophoresis. These bands migrated more slowly than expected from the Mr calculated on the basis of the nucleotide sequence of the gene. Synthesis of the E1a protein caused induction of a specific family of heat-shock proteins (Hsp70), which, however, did not confer heat resistance to the yeast. In addition, the E1a production resulted in an elongation of the generation time of yeast from 2.4 h to 3.9 h, which was attributed specifically to elongation of the G1 interval in the cell cycle. In the light of these findings, we suggest that the E1a protein synthesized in yeast exerts a specific function.

Adenovirus Early Proteins↗
PubMed1987

Decrease in catalase activity of cultured cells by Mycoplasma gallisepticum infection.

The effect of Mycoplasma gallisepticum infection on the host cell catalase activity was histochemically examined in cultured chicken embryo fibroblasts (CEF) and kidney cells. The activity in normal cells was detected as fine, brown granules in the cytoplasm, which appeared ultrastructurally to correspond to anucleoid microbodies. By infecting cultured cells with a CEF-passaged strain of M. gallisepticum, the catalase-positive granules clearly decreased in amount, whereas the UV light-killed mycoplasma and the original strain failed to decrease the granules. The cell-passaged strain was able to induce cytopathic effects and this appeared to be due to its enhanced adherent ability as compared with the original strain. These findings suggest that attachment of viable organisms to cells is crucial to decrease the catalase activity and that the decreased activity may be an important process for the subsequent development of cytopathic effects.

Animals↗
PubMed1987

[Effect of platonin on bone wound healing in rat calvaria--with special reference to the interaction of platonin and steroid hormones].

Interaction of Platonin (PI) and dexamethasone (DM) or testosterone propionate (TP) on bone wound healing was studied by measuring the areas of wound holes which were made in the rat parietal bone. The result was compared with that of the growth of the femur. 1) No significant difference was observed between the control group and pl groups (1, 10 and 100 micrograms/kg, s.c., for 4 weeks) on the wound hole area or the length, weight, calcium content (Ca) or hydroxyproline content (HP) in the femur. 2) The bone wound healing was delayed by DM (2 mg/kg, s.c., for the first 2 weeks). The inhibition of growth was also observed in the femur length and weight, but no significant effect of DM was observed in the Ca and HP of the femur. The combination of Pl and DM promoted the recovery from delayed bone wound healing and femur weight gain caused by DM. 3) No significant effects of TP (4 mg/kg, s.c., for the first 2 weeks) were observed on the wound healing and the femur growth, but an increase of the femur weight and Ca was observed by the combination of Pl and TP. These results indicate that Pl promotes the recovery from delayed bone wound healing and femur weight gain by DM, although no significant effects were observed in bone growth and bone wound healing by the administration of Pl alone. It is also suggested that a combination of Pl and TP promotes bone growth and mineralization.

Animals↗
PubMed1987

Observations on soft tissue calcification in DBA/2NCrj mice in comparison with CRJ:CD-1 mice.

Male and female DBA/2NCrj (DBA/2) mice 3, 4, 5 and 10 weeks old were examined biochemically and pathologically and the results obtained were compared with those for CRJ:CD-1 (ICR) mice of the same age. The plasma levels of glucose, triglyceride and total cholesterol tended to be lower in DBA/2 mice than in ICR mice but the levels of non-esterified fatty acid, calcium and inorganic phosphorus were almost the same in the two strains. The mean body weight of DBA/2 mice was significantly lower than that of ICR mice at each examination, and the relative weights of the hearts of male and female DBA/2 mice were significantly greater than those of male and female ICR mice. Cardiac calcinosis, tongue calcification and corneal degeneration occurred exclusively in DBA/2 mice with incidences of 30%-100%. The incidence and severity of these lesions increased with age but no sex differences were seen. It was difficult to relate differences in biochemical features of the two strains with pathological findings obtained in the DBA/2 mice. The numbers of cells secreting adrenocorticotropic hormone in the pituitary glands were significantly greater in male and female DBA/2 mice than in ICR mice, suggesting a higher secretion of corticosteroids in the former strain.

Animals↗