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Biomedical subjects

M Tabata

Publications and source records attributed to M Tabata.

At least 289 records · Page 16Linked to original sources
PubMed1979

Use of immobilized enzymes in automated clinical analysis: determination of uric acid and glucose using immobilized enzymes in column form.

We studied the use of immobilized enzymes, covalently bound to alkylaminosilane derivative of porous glass, to automated clinical analysis on uric acid and glucose in blood, serum and urine. A microcolumn with an immobilized enzyme was prepared and used in an AutoAnalyzer I continuous flow system. Uricase (EC 1.7.3.3) from Candida utilis and glucose oxidase (EC 1.1.3.4) from Aspergillus niger were immobilized for the determination of uric acid and glucose, respectively. Hydrogen peroxide produced by these oxidases was colorimetrically determined using horse-radish peroxidase (EC 1.11.1.7) and a hydrogen acceptor in solution. Sensitivity and wash charactertistics of a column with immobilized enzyme, 1.5 mm of inner diameter and up to 40 mm in length, were satisfactory at an assay speed of 50 samples per hour. The results correlated well with those obtained by other well established methods utilizing the AutoAnalyzer system. The immobilized enzymes were sufficiently stable for at least two months of 2000 tests when used repeatedly. Clinical trials proved that this method is capable of replacing the soluble enzyme method, giving reliable and reproducible results at lower cost.

Autoanalysis↗
PubMed1977

Role of the posterior hypothalamus in the development of acute brain swelling.

Destructive stereotaxic lesions were made in the posterior hypothalamus, unilaterally or bilaterally, in 26 dogs. In 21 dogs the intracranial pressure (ICP) was maintained in normal range, and in five dogs the ICP was artificially elevated to 300 to 400 mm h2o, preceding the procedures to the hypothlamus. Arterial oxygen and carbon dioxide pressure ((PaO2 and PaCO2) were maintained in the normal range. Before and after each procedure, systemic arterial pressure (SAP) was elevated by intravenous injection of norepinephrine (5 X 10(-3) mg/kg) to determined whether the ICP increases coincidentally with elevation of the systemic arterial pressure. The intracranial pressure/mean arterial pressure ratio of elevation by injection of norepinephrine was not significant regardless of the level of the ICP, or of uni-or bilateral lesions of the hypothalamus. The authors conclude that dysfunction of posterior hypothalamus does not play a specific role in the development of vasomotor paralysis leading to acute brain swelling, under conditons of normal or moderately raised ICP with normal PaCO2 and Pato2 levels.

Animals↗
PubMed1977

Anti-nociceptive substances from the roots of Angelica acutiloba.

Anti-nociceptive constituents of the Chinese crude drug Toki (the roots of Angelica acutiloba var. acutiloba Kitagawa) were investigated by means of chemical fractionation and bioassay. Seven active compounds that inhibit AcOH-induced writhing in mice have been isolated from Toki for the first time and identified as falcarinol, falcarindiol, falcarinolone (polyacetylenes), choline, scopoletin, umbelliferone and vanillic acid. Among these, the three polyacetylenes were found to be most active in the writhing test. Falcarindiol and choline also showed anti-nociceptive activities in the retrograde injection test of bradykinin into a carotid artery on rats.

Analgesics↗