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Biomedical subjects

M Szmitkowski

Publications and source records attributed to M Szmitkowski.

87 records · Page 5Linked to original sources
PubMedDate not supplied

[Granulocyte colony stimulating factor (G-CSF) in diagnosis and monitoring of non-small-cell lung cancer (NSCLC)].

Granulocyte-colony stimulating factor (G-CSF) is one of the glycoproteins called colony-stimulating factors (CSFs). It has been shown that the target of the actions of CSFs are not limited to hematopoietic cells but can also affect the proliferation of nonhematopoietic cells. Some clinical investigations have shown the presence of cell surface receptors for G-CSF in lung cancer cells and autologous production of G-CSF in various human cell lines derived from non-small-cell lung cancer (NSCLC). The purpose of this investigation was to compare serum levels of G-CSF in NSCLC patients to a control group, to assess pre- and post treatment levels of G-CSF in relation to levels of commonly accepted tumour markers such as carcinoembryonic antigen (CEA) and cytokeratin fragment 19 (CYFRA 21-1), and to define the sensitivity of G-CSF in NSCLC. In this study, the serum levels of tumour markers were measured in 34 patients with NSCLC and in 20 healthy subjects. Serum samples were drawn before surgery and 10, 30, 90, 180 and 270 days after surgery. G-CSF and CEA were assayed using ELISA system and CYFRA 21-1 was measured by radioimmunoassay (RIA). Preoperative level of G-CSF was significantly increased in cancer patients relative to the control group. Concentrations of G-CSF and CYFRA 21-1 were decreased on the 10th day, but CEA on the 30th day after operation. The diagnostic sensitivity of G-CSF was 66%, CEA--62% and CYFRA 21-1--51%. Combined use of two markers increased the sensitivity in comparison to the use of G-CSF only. These results suggest that G-CSF may be useful in diagnostic and monitoring of NSCLC, but they need further studies.

Adult↗
PubMedDate not supplied

The effect of cytotoxic drugs on the development of murine granulocyte-macrophage (GM) progenitor cells.

The effect of cytotoxic drugs was tested on the hematopoietic system by assay of the ability of granulocyte-macrophage colony forming cell (GM-CFC) in mice to create GM colonies. The in vivo ability of bone marrow progenitor cells to granulocyte-macrophage colony formation, was tested after long-term peritoneal cytotoxic drug administration. The direct effect of these agents on cells in vitro culture was evaluated also. It was found that cytotoxic drugs inhibit the GM colony formation. The degree of damage to the bone marrow progenitor cells by assaying in vivo colony formation inhibition, depends on the drug dosage and length of therapy (after 25-30 days of treatment the colony growth was below 50%). The in vitro inhibition of granulocyte-macrophage colony formation depends on the concentration of drug (10(-7)-10(-5) M is critical for GM colony growth). The results suggest the possibility of the GM-CFC growth testing as an indicator of the progress or side effects of cytotoxic therapy.

Animals↗
PubMedDate not supplied

The effect of glucocorticoids on the development of murine granulocyte-macrophage (GM) progenitors.

The effect of glucocorticoids was tested on the hematopoietic system by assay of the ability of granulocyte-macrophage colony forming cells (GM-CFC) in mice to create GM colonies. The in vivo ability of bone marrow progenitor cells to granulocyte-macrophage colony formation was tested after long-term peritoneal glucocorticoids administration. The direct effect of these agents on cells in vitro culture was evaluated also. It was found that glucocorticosteroids inhibit the GM colony formation. The degree of damage to the bone marrow progenitor cells assaying by in vivo colony formation inhibition depends on the drug dosage and the length of therapy. The in vitro inhibition of granulocyte-macrophage colony formation depends on the concentration of the drug. The results suggest the possibility of the GM-CFC growth testing as an indicator of the side effects of prolonged corticoid therapy.

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[Enzymatic diagnosis of alcoholism-induced damage of internal organs].

++Post-alcoholic lesion of liver, pancreas, and heart muscle was estimated by measurement of some enzymes activity. Alcoholic in-patients were divided into two groups in regard to the age and the length of the disease. The activity of enzymes in the blood was measured by kinetic methods using the RA-1000/Technicon analyser. It was shown that the increase of activity of alanine aminotransferase (AlAT), gamma-glutamyltransferase (GGTP), and alcohol dehydrogenase (ADH) may indicate the ++post-alcoholic liver damage, while increase of activity of alpha-+-amylase and ++leucine aminopeptidase (LAP) may be useful for the diagnosis of pancreas lesion, and creatine kinase (CK) as well as lactate dehydrogenase (LDH) for the evaluation of postalcoholic lesion of the heart muscle.

Adult↗
PubMedDate not supplied

[Effect of sera from children with acute lymphoblastic leukemia on the morphology of granulocyte-macrophage colonies grown from murine bone marrow cells and the cells of murine myelomonocytic leukemia WEHI 3B D+].

Active role of the T lymphocytes in the regulation process of the granulo- and monocytes colonies formation has been recently emphasized. The cells are a source of number of factors stimulating proliferation and differentiation of both, the steered stem cells and multipotential cells. The possibility of the liberation by some classes of T lymphocytes of substances with inhibitory faculties is also recently suggested. Such vast function of these cells in the process of granulo- and monocyte formation in normal conditions brings forward the question of their role in the proliferating diseases of the lymphatic system especially in connection with some research indicating the presence of changes of the regulators in the course of proliferating diseases of the bone marrow. Taking into account above data investigations have been carried on aimed at the demonstration to what degree stimulators or inhibitors which can be present in the blood serum of children with acute lymphoblastic leukemia are able to determine the composition and morphology of colonies arising from stem cells of bone marrow and of the cells of myelomonocytic leukemia of WEHI 3B,D+ line. This creates the possibility of determination of the nature of the examined substances and of the effect to the treatment on their concentration. The activity of the regulators has been examined in the semi-liquid agar culture "in vitro" applying the Bredley and Metcalf method. The effect of the treatment applied on the investigated parameters has been also assessed. In children with acute lymphoblastic leukemia prior to the initiation of treatment, the increased activity distinctly stimulating the growth of granulocytic and only slightly of macrophage colonies has been present.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMedDate not supplied

[Human serum colony-stimulating factor in mouse bone marrow culture].

Activity of CSF was measured in human serum after chromatography on Sephadex G-200. Agar culture of bone marrow cells was done in Petri dishes with agar in Eagle'a medium containing fetal calf serum and antibiotics. This agar-medium containing mouse bone marrow cells, was allowed to gel and the dishes were incubated at 37 degrees C in a fully humidified atmosphere of 10% CO2 in air. Our data indicate that chromatography procedure allows to get CSF free from its inhibitors. All activity was localized in one peak, in the region of low molecular weight proteins.

Bone Marrow Cells↗
PubMedDate not supplied

[Colony-stimulating factor (CSF) and granulopoietic activity (GA) in various mouse organs].

The colony stimulating factor and granulopoietic activity were determined in the homogenates of mouse kidney, liver, spleen and bone marrow. The activity of CSF and GA were highest in the kidney. The homogenate of this organ was fractionated then by ultracentrifugation. The distribution of CSF and GA in subcellular fractions and homogenates may suggest that kidney cells are the source of a substance causing, both, colony stimulation and granulopoietic activity.

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