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Biomedical subjects

M Szmitkowski

Publications and source records attributed to M Szmitkowski.

At least 73 records · Page 4Linked to original sources
PubMed1985

Serum lipoproteins inhibitors of granulocyte-macrophage (GM) colony formation.

Normal and chloroform-extracted human sera, fractionated by Sephadex column chromatography, were tested for inhibitory activity on granulocyte-macrophage (GM) colony formation. This activity was found to be connected with lipoproteins with a molecular weight of about 200,000. Serum native fractions of lipoproteins were isolated and mainly high density lipoproteins (HDL) and very low density lipoproteins (VLDL) were shown to have an unspecific inhibitory activity directed on colony stimulating factor (CSF) action.

Granulocytes↗
PubMed1984

Tissue inhibitors of granulocyte-macrophage (GM) colony formation.

The effect of mice tissue homogenates on the inhibition of granulocyte-macrophage colony formation in vitro was studied. The potent inhibitory activity was displayed by homogenate from liver. The lowest values were found in the kidney and heart. An interrelationship between the rate of inhibition and protein concentration in the homogenates was noted. Biological role and the type of inhibitory activity are briefly discussed.

Animals↗
PubMed1984

Inhibition of granulocyte-macrophage (GM) colony formation by sera of patients with neoplasma of digestive tract in the course of surgical treatment.

The effect of sera from patients with neoplasm of the digestive tract obtained during intervention has been tested on granulocyte-macrophage (GM) colony formation. Independently of the source of blood (peripheral or tumour draining vein) and the time of sampling (during or 2-3 weeks after operation) the tested sera suppressed GM colony formation. The highest decrease was caused by blood sampled from the vein draining the tumour. It is concluded that tumour cells may be a source of the GM colony formation inhibitory activity. The possible character of the activity is discussed.

Adult↗
PubMed1983

Serum inhibitory activity on granulocyte-macrophage colony formation.

Normal and chloroform-extracted human sera, fractionated by Sephadex column chromatography, have been tested for inhibitory activity on granulocyte-macrophage (GM) colony formation. It was found that this activity is connected with lipoproteins (inhibitors of granulocyte-macrophage colony stimulating factor) and low molecular weight substances (7000; 13,000) which can act as specific polypeptide chalones.

Animals↗
PubMed1980

Granulopoietic activity (GA) and colony stimulating factor (CSF) in myeloproliferative diseases.

Granulopoietic activity (GA) in vivo and colony stimulating factor (CSF) in vitro were measured in sera of patients with acute and chronic myeloid leukaemia, chronic lymphatic leukaemia and Hodgkin's disease. Before testing sera were dialysed for removing unspecific lipoproteid inhibitors of CSF. We have found a lower activity of CSF in these diseases than in the control group. GA was even below zero stimulation especially in chronic leukaemias. This confirms the presence of inhibitory effect of the tested sera on granulopoiesis in vivo and indicated that GA tested in vivo may be more favourable for the prognosis of leukaemias.

Animals↗
PubMed1978

Distribution of CSF (colony stimulating factor) in kidney of the mouse.

The activity of the colony stimulating factor (SCF) was measured in kidney subcellular fractions in mice. The highest activity was noted in microsomes. From other fractions, the cytosol had large amounts of CSF. On the basis of literature data, and the findings presented we suggest that the kidney is at least one of the organs of CSF biosynthesis.

Animals↗
PubMed1978

Colony stimulating factor (CSF) activity in human and murine sera.

Substances with CSF activity were studied in human and murine sera. Gel filtration of the sera on Sephadex G-200 showed that fractions with molecular weight of 14,000 and 6000 D were active in the CSF test. When mice were stimulated with endotoxin, CSF activity of both fractions was twice as high as in control animals, and CSF activity was found also in a protein peak with the molecular weight of 300,000 D. The fractions with CSF activity were sensitive to pepsin.

Animals↗
PubMed1978

Is granulopoietic activity (GA) in vivo comparable to colony stimulating factor (CSF)?

Human sera after column chromatography on Sephadex G-200 were tested for Granulopoietic Activity (GA) in vivo and Colony Stimulating Factor (CSF) in vitro. We have found that GA after chromatography is distributed in three peaks but CSF only in one peak with high activity, and evident activity in volume 135 ml. This activities are in good agreement with two peaks of GA. We assume that GA and CSF after this chromatographic procedure is free from lipoproteins inhibitors. The obtained results are briefly discussed.

Animals↗
PubMed1977

Antihaemophilic factor (factor VIII) from human granulocytes.

Factor VIII has been isolated and purified from human granulocytes using chromatography on Sephadex G-200 AND DEAE-Sephadex A-50. The final preparation was purified 60-fold. Some properties of this partly purified factor VIII were compared with the human plasma AHF preparation. Optimum pH activity and the highest activity after incubation at different pH values was found in buffer at pH 7.0. The preparation was thermolabile and had a molecular weight of about 214,000. Absorption curves indicated that it is a protein exhibiting four fractions in polyacrylamide gel disk electrophoresis. AHF preparations from plasma and granulocytes were almost identical in the characteristics tested.

Chromatography, Ion Exchange↗
PubMedDate not supplied

[CDT (desialylated transferrin)--a new biochemical marker of alcohol abuse].

Serum concentration of carbohydrate-deficient transferrin (CDT) is used for laboratory diagnosis of chronic alcohol abuse. Several earlier studies reported sensitivities of 90% or above for CDT, with a specificity of 90-100%, although other investigators found lower sensitivities. In general, CDT has been reported to be highly specific (92%) and relatively sensitive (80%) for the detection and monitoring of alcoholism. There are no correlation between CDT concentration and gamma-GT activity. Any alteration in serum total transferrin concentration markedly decreases the CDT assay specificity. This should be considered when interpreting the assay results in patients with elevated serum transferrin. There are differences between the CDT isoforms (asialo-Tf and monosialo-Tf) in males and females relative to alcohol consumption. Alcohol consumption increases the levels of asialo-Tf and monosialo-Tf in women more strongly than in men. Sensitivity of CDT assay is also related to age of patients. There is a significantly higher sensitivity of CDT in patients above 40 years of age as compared to younger patients. The measurement of carbohydrate-deficient transferrin may be used as a marker of excessive alcohol abuse in patients with liver diseases (also in cirrhosis). The specificity of CDT in patients with non-alcoholic liver disease was consistently higher than that of gamma-GT (80% vs 60%). Disulfiram therapy during detoxification does not influence the serum level of CDT.

Adult↗