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Biomedical subjects

M Sun

Publications and source records attributed to M Sun.

At least 253 records · Page 14Linked to original sources
PubMed1991

Effect of prior immunity on the shedding of virulent revertant virus in feces after oral immunization with live attenuated poliovirus vaccines.

Groups of infants were immunized with one or two doses of orally inoculated live attenuated Sabin poliovirus vaccine (OPV group) or with one or two doses of enhanced-potency inactivated poliovirus vaccine (EIPV) administered parenterally followed by one or two doses of OPV (EIPV-OPV group). The fecal specimens from both groups were tested for poliovirus shedding 1-2 months after OPV. The virus isolates were examined for nucleic acid sequences in the 5' noncoding regions (bases 480, 481, and 472 for serotypes 1, 2, and 3, respectively) to determine whether the viruses shed represented nonattenuated revertants, attenuated parent vaccine strains (nonrevertants), or both. In the OPV group, 4 of the 6 virus isolates recovered 30-60 days after the first immunization dose and 1 of the 3 isolates obtained after the second dose were found to be nonrevertants (parent vaccine strain). In contrast, 11 of the 12 isolates in the EIPV-OPV group were of the nonvaccine revertant virus types. The frequency for reversion appeared to differ for different poliovirus serotypes. However, all revertant type 3 isolates were recovered from subjects previously immunized with EIPV.

Antibodies, Viral↗
PubMed1991

[Effects of noxious stimuli on the discharges of pain-excitation neurons and pain-inhibition neurons in the nucleus ventralis posterolalis of thalamus in the rat and a modulating action of electroacupuncture on its electric activities].

The somatic and visceral noxious stimuli could simultaneously induce the increase of discharges of pain-excitation neurons (PEN) and the decrease of discharges of pain-inhibition neurons (PIN) in the Nucleus Ventralis posterolalis (VPL) of thalamus in the rat, and the electric activities of the two kinds of neurons cooperated each other. By the injection of morphine (5 mg/kg) and the electroacupuncture of "zusanli", the discharges of PEN were reduced and the discharges of PIN were enhanced. The results suggested that VPL of thalamus in the rat be involved in the modulating action on pain sensation and the analgesic effects of morphine and electroacupuncture by the simultaneous electric activities of PEN and PIN in VPL.

Animals↗
PubMed1990

Computer analyses of EEG-sleep in the neonate: methodological considerations.

Neonatal EEG interpretation can aid in the estimation of central nervous system maturation, as well as provide diagnostic and prognostic information of the high-risk infant. However, one cannot easily visualize the complex interrelationships coupling EEG and polysomnographic components of the EEG-sleep rhythm. This is particularly relevant for the preterm neonate, in whom a rudimentary sleep cycle has not yet been clearly delineated. Computer analysis can augment the information derived from the visual interpretation of scalp-generated EEG activity. Automated techniques for EEG-sleep analysis have only recently been applied to a neonatal population. Such studies have been limited to full-term rather than preterm infants and rely on conventional methods that assume stationarity of neurophysiologic signals. We describe a computer system that simultaneously compares behavioral and electrographic components of EEG-sleep in a manner that preserves the integrity of the signals over time, while investigating the time- and frequency-dependent relationships among signals. Strategies for on-line and off-line editing, data storage, and off-line signal processing are described. Computational algorithms regarding analyses of EEG power, motility, and cardiorespiratory data are being used to study the ontogeny of EEG-sleep in asymptomatic preterm and full-term neonates. Computer strategies are based on both principles of stationarity and nonstationarity of physiologic signals and are applied depending on the temporal resolution required for specific signal processing needs.

Computer Systems↗
PubMed1990

Acoustic recordings from experimental saccular aneurysms in dogs.

In an effort to isolate and characterize the emission of acoustic signals from saccular aneurysms, we made a series of invasive microphone recordings from experimental aneurysms created on the common carotid arteries of dogs using the vein pouch technique. Using a modified probe microphone, we compared recordings from the common carotid artery before creation of the aneurysm to recordings from the aneurysmal surface, both before and after clip occlusion. We then performed spectral analysis, band-pass filtering, and spectrographic analysis to compare the dominant frequency and width of the frequency range of both the aneurysmal and carotid recorded signals. The aneurysmal signals had a significantly higher dominant frequency (p less than or equal to 0.05) and a significantly wider frequency range (p less than or equal to 0.05) than the carotid signals. Aneurysmal signals recorded under conditions of systemic hypotension had a significantly lower frequency (p less than or equal to 0.05) than aneurysmal signals recorded under conditions of hypertension. Our results support the assumptions that acoustic signals from experimental saccular aneurysms are distinct from those of the parent vessel and that the aneurysmal signal can be characterized using passive microphone recordings.

Animals↗
PubMed1990

[Modified radical mastectomy in the treatment of stage III breast carcinoma].

From 1972 to 1982, 237 patients with stage III breast carcinoma were admitted into our institute. One hundred and eleven cases underwent Auchincloss and Madden modified radical mastectomy (MRM), and the remaining 126 cases received Halsted radical mastectomy (RM). The 5 and 10 years survival rates were 59.17% (61/111), and 36.92% (24/65) in MRM group. The 5 and 10 years survival rates were 53.17% (67/126) and 29.31% (17/58) in RM group. There were no statistically significant differences in the long-term survival rates between the two groups (P greater than 0.05). The authors believe that MRM causing less trauma should be considered in the treatment of stage III breast carcinoma unless in cases with major pectorial muscle involvement or large metastatic axillary nodes.

Adult↗
PubMed1990

[Effects of alpha-human ANF and angiotensin II on aldosterone secretion in vitro from cultured human adrenal tissues and APA tissues].

The effects of the alpha-human ANF and angiotension II on aldosterone secretion in vitro from cultured human adrenal tissues and the aldosterone-producing adenoma (APA) tissues were studied. The fresh human normal adrenal and APA tissues were obtained surgically from five patients. The tissues were mined 1.0 mm3 with scissors, put in DMEM containing 0.25% trypsin, and digested at 37 degrees C for 10 min. Then the tissues were washed with DMEM. The tissues were incubated in 4 ml DMEM containing 10% fetal calf serum at 37 degrees C under 5% CO2 in air for seven days and the aldosterone levels of the culture medium were determined by radioimmunoassay (RIA). The experiments were started on the tenth to fiftieth day of culture. The results show that the effect of alpha-human ANF (10(-8) mol/L final concentration) on aldosterone secretion from normal adrenal tissues was increased at 30 min following a decrement, but do not inhibit the aldosterone secretion in APA tissues. The aldosterone of short duration was inhibit by alpha-human ANF (3 x 10(-8) mol/L or 5 x 10(-8) mol/L) and the aldosterone secretion in a dose-dependent manner in the adenoma tissues. The aldosterone responses were not stimulated by angiotensin II (10(-9) mol/L) in normal adrenal and adenoma tissues, but angiotensin II (5 x 10(-9) mol/L) can stimulate APA tissues. Our results indicate the lack of effect of alpha-human ANF and angiotensin II on APA tissues could be due to the absence of receptors or a variance of the receptors, and/or the enzymes of steroidogenesis were abnormal. These tissues had a marked aldosterone response to ANF and angiotensin II during culturing in one month.

Adenoma↗
PubMed1990

[Survey of Bacillus thuringiensis and Bacillus sphaericus from soils of four provinces of China and their principal biological properties].

A number of isolates of Bacillus thuringiensis and Bacillus sphaericus were obtained from soils of Southwestern Area and Shaanxi Province of China. Among isolates of B. thuringiensis were under 13 sorts of serotype in total of 23 sorts of B. thuringiensis and about 20% of auto-agglutinate strains. Rules of ecologic distribution of two sorts of bacteria were analysed. Toxicities on six species of insects, morphology and crystal proteins of B. thuringiensis, as well as toxicities, morphology and crystal proteins of B. sphaericus, were investigated. 22 strains of more efficient of B. thuringiensis and 2 strains of more efficient of B. sphaericus were obtained. It was shown that B. thuringiensis is actually soil microorganism, and resource of B. thuringiensis is much fruitful in Southwestern Area of China.

Bacillus↗
PubMed1990

[Modified radical mastectomy--an analysis of 217 cases].

Modified radical mastectomy (MRM) was performed in two hundred and seventeen patients with operable breast cancer during 1972-1982. Having been followed for 5 to 10 years, the results were compared with those of radical mastectomy (RM) during the same period. There was no significant difference in 5 and 10 year survival rates between the two groups. MRM had the advantages of insignificant deformity, better function and easy breast reconstruction in comparison with RM. The authors believe that MRM should be recommended as the treatment of choice for breast cancer.

Adenocarcinoma↗
PubMed1989

Nasopharyngeal secretory antibody response to poliovirus type 3 virion proteins exhibit different specificities after immunization with live or inactivated poliovirus vaccines.

By using immunoblotting, neutralization, and ELISA, the development of secretory antibody responses to poliovirus type 3 virion proteins (VP1, VP2, VP3) and to intact or trypsin-treated poliovirus type 3 was studied in the nasopharyngeal secretions in groups of infants after immunization with live attenuated poliovirus vaccine (OPV), enhanced potency inactivated poliovirus vaccine (IPV-EP), or after combined vaccination with IPV-EP followed by OPV. After three doses of vaccine, infants in all vaccine groups developed similar secretory IgA response to VP1 and VP2. The antibody response to VP3 was observed in 76.5% of subjects immunized with OPV alone and approximately 60% of those immunized with IPV-EP followed by OPV. However, only 13% of those immunized with IPV-EP alone exhibited VP3-specific antibody response. Significant differences in poliovirus type 3 specific antibody activity were observed between OPV and IPV-EP immunized subjects when trypsin-treated poliovirus was used as the antigen for neutralization or for ELISA in vitro. The neutralizing antibody activity against cleaved virus was significantly higher than against whole virus in the OPV vaccinated subjects. Both neutralizing and ELISA antibody activity against cleaved virus was significantly lower than against the whole virus in IPV-EP immunized subjects.

Antigens, Viral↗