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Biomedical subjects

M Sulik

Publications and source records attributed to M Sulik.

At least 37 records · Page 2Linked to original sources
PubMed1995

The effect of activated alveolar macrophages on experimental lung emphysema development. II. The study of fibroblast and alveolar macrophage co-culture.

The cell-cell interaction between fibroblasts and alveolar macrophages was examined using a co-culture system. Alveolar macrophages (AM) were harvested from the bronchoalveolar lavages (BAL) of rats with papain induced lung emphysema. The BCG-vaccine was applied as a macrophage mobilizing and activating agent. The morphological examinations carried out in scanning electron microscope (SEM) as well as the evaluation of the uptake of 3H-thymidine did not show any significant differences between respective co-cultures of fibroblasts and AM isolated both from the lungs of control and experimental animals (treated with BCG or papain, and BCG+papain). However, significant growth were noted in 3H-thymidine uptake between fibroblast cultures done with or without cells isolated from the lungs. The results obtained suggest that AM can promote fibroblast proliferation during the progression of experimental lung emphysema.

Animals↗
PubMed1995

The effect of activated alveolar macrophages on experimental lung emphysema development. III. Morphological analysis of the lung tissue and alveolar macrophages in situ.

Morphological (in light and transmission electron microscope) as well as morphometrical analysis of the lungs was performed on experimental, papain-induced lung emphysema. Development of emphysematous changes was studied seven days after a single intratracheal instillation of papain solution. The effect of alveolar macrophages (AM) activation by BCG-vaccine on changes in pulmonary tissue was analyzed. In the rats given BCG the number of AM increased and demonstrated enhanced activity. Increase in reticulin fibre density in places of AM cumulation, particularly in BCG+papain-treated rats was observed. The lungs of animals treated with BCG+papain showed enhancing of emphysema comparing with the papain-treated rats. Development of emphysematous changes, especially in BCG+papain-treated rats coexisted with cumulation of activated alveolar macrophages and collagen fibres as well as type II alveolar epithelial cells proliferation. Our data support the inflammatory-repair hypothesis of emphysema pathogenesis and indicate that AM regulate collagen production in the lung. Type II alveolar epithelial cells seem be important in lung injury and repair.

Animals↗
PubMed1995

Cor pulmonale in experimental lung emphysema. I. The morphometric evaluation of pulmonary and myocardium changes.

Selected morphometric parameters of the lungs and heart of rats with experimental emphysema caused by a proteolytic enzyme--papain have been presented. The morphometric evaluation was carried out after the 1st, 3rd and 6th months following an intratracheal infusion of papain solution. It was found that administration of the papain caused emphysematous changes in the lungs, which intensity measured morphometricly increased until the third month, and then became stable. The first evidence of the right ventricle hypertrophy appeared already in the third month of the experiment. The evident hypertrophy of the muscle of both ventricles and of the interventricular septum was observed after six months of the experiment. Morphologic changes developing in both ventricles suggest an existence of a common pathogenetic factor affecting simultaneously the myocardium of right and left heart ventricles.

Animals↗
PubMed1993

Morphological pattern of the hypothalamo-neurohypophyseal system in acute intoxication with 2,4-dichlorophenoxyacetic acid [2,4-D].

The amount and distribution of the neurosecretory material in the hypothalamo-neurohypophyseal system were investigated in rats sacrificed 24 hours, 3 or 14 days after single intragastric administration of 2,4-D acid or physiological solution. The amount of the neurosecretory material increased in the whole system after 24 hours and then it decreased on the 3rd and on the 14th day after administration of 2,4-D acid.

2,4-Dichlorophenoxyacetic Acid↗
PubMed1993

[Residues of chlorinated hydrocarbons in livers of newborns, infants and children].

In the liver of 4 newborns, 2 infants and 2 children the levels of residues of chlorinated hydrocarbons were determined. The highest levels of sigma HCH (817 mcg/kg of fat, 17.1 mcg/kg of tissue) were found in a 2-day-old newborn, the lowest ones (43 mcg/kg of fat, 1.8 mcg/kg of tissue) were in an infant aged 12 months. The content of sigma DDT was highest (3044 mcg/kg of fat, 93.8 mcg/kg of tissue) in the liver of an infant aged 13 days, the lowest values (322 mcg/kg of fat, 5.6 mcg/kg of tissue) in the liver of a child aged 12 years. The results are comparable to those obtained in this country in the liver of adults and in human placenta.

Child↗
PubMed1991

[Lead contents in liver and kidney of inhabitants of the city of Białystok and vicinity].

The material comprised liver and kidney samples collected from inhabitants of the city of Białystok and of its vicinity during anatomopathological examination at the Department of Pathological Anatomy, Medical Academy in Białystok. In age groups: 2 days-8 years, 29-65 years, 66-84 years, the mean liver lead content was 0.220, 0.211 and 0.233 mg/kg, respectively. The mean kidney lead content amounted to 0.272, 0.038 and 0.125 mg/kg, respectively. When compared with the literature data for subjects not exposed to lead, the present results have to be regarded as low. The newborn displayed a higher kidney lead level than adults. In contrast to adults, in the newborn the content of lead in the kidney exceeded that in the liver. Adults showing a higher lead level in the liver than in the kidney exhibited a correlation between Pb level in the liver and kidney (r = 0.671).

Adult↗
PubMed1990

[Atrophy of the bone marrow].

The authors made a quantitative analysis of the active hematopoietic tissue of the bone marrow with particular consideration of its atrophy in the course of various diseases. The material consisted of 407 non-selected autopsy cases. For a morphometric analysis the bone marrow was sampled from the sternum, ala ossis illi and spine. In the quantitative analysis of the active hematopoietic tissue we took into account age groups as quantitative changes appear with age. Atrophy of the bone marrow was in 19.4% of the studied cases. The presence of bone marrow atrophy was found in the course of various diseases, most frequently neoplastic, particularly in patients aged from 50 to 59 years.

Adolescent↗
PubMed1990

[Gelatinous transformation of the bone marrow].

The incidence and histopathologic picture of gelatinous transformation of the bone marrow were analysed in non-selected autopsy material. It was found that gelatinous transformation of the bone marrow occurred in terminal stages of various diseases (malignant neoplasms, chronic inflammation). Histological studies showed that gelatinous transformation of the bone marrow led to atrophy of the hematopoietic and adipose tissues of the bone marrow and accumulation of acid mucopolysaccharides. The patients with gelatinous transformation of the bone marrow exhibit hematologic disorders, most frequently anemia and thrombocytopenia.

Aged↗
PubMed1990

[Distribution and elimination of C-14-2,4-dichlorophenoxyacetic acid (2,4 D) in rat tissues in acute poisoning].

The authors carried out investigations for elucidating the distribution in the internal organs and tissues and the rate of the absorption from the digestive tract and elimination from the organism of 2,4-dichlorophenoxyacetic acid widely used in herbicides, to which man and animals are exposed. Radiometric investigations were carried out of the internal organs and tissues of male Wistar rats which were given one dose of the acid labelled with 14C one-third of LD-50/kg through an intragastric tube. The rats were killed at various time intervals for observing the rate of absorption and elimination of this compound. The measurements of radioactivity demonstrated high levels of the herbicide in the lungs, heart, liver, spleen and kidneys, and low levels in the adipose tissue and brain, with intermediate values in the adrenals and testicles. The compound is rapidly absorbed from the gastrointestinal tract reaching its highest level in the tissues after 6-20 hours, and is eliminated nearly completely already on the second day. Small residues of the acid and its metabolites containing 14C were present in the tissues up to the 20th day of the experiment.

2,4-Dichlorophenoxyacetic Acid↗
PubMed1989

[Congenital anomalies of the central nervous system in autopsy specimens].

On the basis of an analysis of 2398 autopsies of infants aged up to 1 year in 194 cases congenital anomalies of the central nervous system were found (8.1%). Most cases of these anomalies were noted in the group of newborns (85%) and the most frequent anomalies were: myelomeningocele (35.6%), multiple anomalies (20.1%), congenital hydrocephalus (17%), anencephaly (14.4%) and corpus callosum malformations (3.6%). Myelomeningocele, congenital hydrocephalus, anencephaly and true microcephaly were more frequent in girls, while multiple anomalies and corpus callosum malformations were more frequent in boys.

Abnormalities, Multiple↗
PubMed1989

[Analysis of neoplasm metastases to the bone marrow in patients with lung cancer].

The authors analysed bone marrow metastases in lung cancer in 104 deceased patients. Trepano-biopsy was taken from the sternum, hip bone and spine. Bone marrow metastases were found in 33 cases (31.73%). Most often they were seen in small cell lung cancer (16 cases--35.56%). In 12 cases the bone marrow was the only site of lung cancer metastases.

Adult↗
PubMed1989

[Chlorinated hydrocarbon residues in the adipose tissue of the population of BiaŁystok and its environs].

In the perirenal fat tissue of 36 representatives of this population (20 men and 16 women aged 41 to 92 years) the levels were determined of the residues of alpha-, beta-, and gamma-HCH, DDE, DDD, DDT and sigma DDT. The obtained mean values were 0.012, 0.013, 0.007, 1.421, 0.035, 0.327 and 1.783 mg/kg of fat, respectively. The obtained results were severalfold lower than those found in Poland in the 1970s. The fat of the human fat tissue contained high amounts of beta-HCH as compared to other isomers of hexachlorocyclohexane, and high amount of DDE in sigma DDT. In the fat of men the residues of DDE and sigma DDT were present in greater amounts than in women. On the other hand, no effect of age was noted on the levels of the residues of chlorinated hydrocarbons in human fat.

Adipose Tissue↗
PubMed1989

[Primary sarcoma of the lung].

The authors present two cases of rare primary lung neoplasms smooth cell sarcoma and fibrosarcoma. Diagnostical dilemmas are discussed.

Adult↗
PubMed1989

[Chlorinated hydrocarbon residues in the livers of the population of Białystok and its environs].

In the livers of 34 subjects living in Białystok and environs the residues of chlorinated hydrocarbons were determined. The mean values of alpha-HCH, beta-HCH, gamma-HCH, DDE, DDD, DDT and sigma DDT were respectively 16, 60, 81, 1090, 94, 177 and 1361 mcg/kg of fat, and 0.5, 3.2, 3.3, 48, 5.0, 7.1 and 60 mcg/kg of tissue. The content of DDE and sigma DDT in liver fat was similar to that found previously in perirenal fat. In relation to perirenal fat the content of DDT in liver fat was lower and that of DDD was higher. This was due, probably, to metabolic changes taking place in the liver. The relationship between beta-HCH and gamma-HCH in the liver was different from that in fatty tissue in which the level of Indian was lower than that of the beta-isomer.

Adipose Tissue↗
PubMed1989

[Sclerosis and fibrosis of the bone marrow in lung and gastrointestinal tract cancers].

The authors analyzed the incidence of pathologic lesions of the bone marrow in the form of sclerosis and fibrosis in patients with lung and gastrointestinal tract (gastric, pancreatic, hepatic and bile duct) carcinomas. In the analysis of argentaffin and collagen fibres in the bone marrow we used Kundel's score modified by Bauermeister. The results obtained in the studied group were compared with the bone marrow of persons without neoplastic diseases and other disorders that might have affected the bone marrow. It was found that in patients with neoplastic disease and fibrosis of the bone marrow were quite frequent disturbances.

Aged↗
PubMed1989

[Pulmonary bone marrow embolism in nonselected autopsy material].

We analyzed the incidence of bone marrow emboli of the pulmonary arteries in nonselected autopsy material involving 620 cases. We evaluated the incidence of these emboli, age, sex, underlying disease and microscopic picture. It was found that bone marrow emboli of the pulmonary arteries occurred in 7% of cases, most frequently in patients aged from 50 to 69 years and they were located in the medium and small-lumen arteries. It was found that bone marrow emboli of the pulmonary arteries may complicate posttraumatic bone fractures, costal and sternal fractures in the course of reanimation as well as malignant neoplasms and shock.

Adult↗
PubMed1989

[Neoplastic metastases in the bone marrow from cancers of the digestive system (stomach, pancreas, liver and gallbladder].

The authors analyse the incidence of metastases into the bone marrow in carcinomas of the stomach, pancreas, liver and gallbladder. Specimens from the bone marrow taken were by trephine biopsy from the sternum, ala ossis ilii and spine. Metastases into the bone marrow were found in carcinomas of the stomach, pancreas and gallbladder but not in hepatic carcinoma. Neoplastic metastases were most frequent in adenocarcinoma of the stomach.

Adenocarcinoma↗