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Biomedical subjects

M Sugimoto

Publications and source records attributed to M Sugimoto.

At least 487 records · Page 27Linked to original sources
PubMed1982

Lack of effect of morphine and noxious stimuli on the MET-enkephalin content in the spinal dorsal horn and nucleus reticularis gigantocellularis of the rat.

The Met-enkephalin contents in the dorsal horn of the lumbar enlargement and the nucleus reticularis gigantocellularis of the medulla oblongata of the rat were measured, using a sensitive and specific radioimmunoassay for Met-enkephalin, and the effects of morphine and noxious stimuli on the Met-enkephalin contents in these regions were examined. In this radioimmunoassay, the IC50 and assayable limits for Met-enkephalin were 45 and 5 fmol/tube respectively, and the IC50 for Leuenkephalin was 0.56 nmol/tube (0.008% cross reactivity between Met-and Leu-enkephalins). The contents of Met-enkephalin-like immunoreactivity in the dorsal horn of the lumbar enlargement and the nucleus reticularis gigantocellularis were not altered by either subcutaneous injection of morphine or thermal (hot plate) and chemical (formalin injection) noxious stimuli applied to the hind-paws.

Animals↗
PubMed1982

The effect of cytochalasin B on the superoxide production by alveolar macrophages obtained from normal rabbit lungs.

Resident alveolar macrophages and lung lavage fluids were obtained from normal rabbit lungs. Superoxide production by alveolar macrophages exposed to lung lavage fluids and cytochalasin B was measured by superoxide dismutase-inhibitable nitroblue tetrazolium (NBT) reduction. Glycogen-elicited peritoneal macrophages were used as a control. Cytochalasin B, as well as lung lavage fluids, enhanced superoxide production by resting alveolar macrophages. The cytochalasin B-induced superoxide production was associated with enhanced attachment to glass and with remarkable alterations of the cell surface morphology, probably relating to interference with the microfilament functions of cells. On the other hand, glycogen-elicited peritoneal macrophages showed only a slight production of superoxide when exposed to the same stimulants.

Animals↗
PubMed1982

Effect of peripheral blood lymphocytes from systemic lupus erythematosus patients on human bone marrow granulocyte precursor cells (colony-forming units in culture).

Because of the possibility that immunological mechanisms might be involved in the pathogenesis of leukopenia accompanying systemic lupus erythematosus (SLE), the effect of lymphocytes on colony-forming units in culture (CFU-C) was compared. The colony formation of the bone marrow CFU-C of SLE patients was less than that of the healthy subjects. Lymphocytes of SLE patients and serum of some of the SLE patients remarkably inhibited colony formation by human bone marrow CFU-C. The results shown above suggest the possibility that immunocompetent cells of the patients are involved in the pathogenesis of leukopenia in SLE.

Adolescent↗
PubMed1981

Asymmetric division of immature lymphoid cells of the embryonic mouse thymus.

Cell division of thymus lymphoid cells from 15- to 19-day-old embryonic mice, as well as from 2- and 7-day-old mice was investigated on cell smears stained with Giemsa or with the Feulgen reaction followed with malachite green. Asymmetrically dividing cells were observed during the development. At the anaphase and telophase of such cells, the nucleus of one of two immature daughter cells was apparently smaller and more condensed than that of the counterpart cell. The asymmetry was already apparent at the level of sister chromosomes at anaphase. Sometimes a difference also existed in the cytoplasm: the cytoplasm of the smaller immature daughter cell with condensed nucleus was stained with Giemsa more deeply than that of the counterpart. The frequency of cells displaying apparent asymmetric division among total dividing cells at the telophase was around 10% between 15 and 19 days of gestation, and decreased to 2% on days 2 and 7 after birth. Asymmetrically dividing cells belonged to immature lymphoid cells of larger size. The biological implications of the present phenomenon are discussed.

Animals↗
PubMed1981

Allorestriction and xenorestriction phenomena in the induction of delayed-type hypersensitivity (DTH) responses in the guinea pig against azobenzenearsonate- (ABA) modified peritoneal exudate cells.

The role of MHC products in the induction of DTH responses Ia antigens play a crucial role in these cells in the recognition of ABA determinants by lymphocytes. Second, JY-1 guinea pigs were employed as responder animals. A clear allorestriction phenomenon was observed again when weakly modified ABA-PEC were used. On the other hand, the allorestriction phenomenon was apparently abrogated when heavily modified ABA-PEC were employed: allogeneic as well as syngeneic ABA-PEC induced strong and comparable DTH responses. Even in this condition, xenogeneic (mouse) ABA-PEC were barely immunogeneic, On the basis of these observations, the cross-reactivity of Ia antigens in the conjunctive recognition of haptenic determinants by T cells is discussed.

Animals↗