Biomedical subjects
Publications and source records attributed to M Sugimoto.
A patient with vesicosigmoid fistula due to "collision" tumor between adenocarcinoma of the sigmoid colon and transitional cell carcinoma of the urinary bladder is presented. Resection of the sigmoid colon and partial cystectomy were performed. The clinical symptoms, diagnostic procedures and treatments are discussed.
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We investigated the effects of morphine on the noxious mechanical stimuli-induced release of immunoreactive substance P (iSP) from the rabbit dorsal horn in vivo. Systemic morphine in a dose of 10 mg/kg, but not 1 mg/kg, inhibited the iSP release induced by noxious stimuli, although both dosages inhibited the nociceptive responses of rabbits to the stimuli. The inhibitory effect of morphine (10 mg/kg) on the iSP release was partially or completely antagonized by the local application of naloxone or prazosin, respectively, to the dorsal horn. The local application of methysergide did not affect the morphine action. The inhibitory effect of morphine (10 mg/kg) on the iSP release was diminished in acute spinal animals. These results suggest that the inhibition of the evoked release of substance P participates only in the action of the larger dose of morphine, and that this inhibition of the substance P release may be mediated by opioid receptors and the noradrenergic system, but not the serotonergic system, in the spinal cord.
To clarify the prevalence of gallstone disease in Bolivia, all the X-ray films of the biliary system in a total of 656 cases taken during 10 months in La Paz were checked. Gallstones were found in 103 cases (15.7%). The ratio of male to female was 1:1.9. Cholecystolithiasis comprised 76.7% of them, while choledocholithiasis was rare. The analysis of gallstones removed surgically by an infrared absorption spectroscopy revealed that cholesterol was main constituent of stones in 62 cases (93.9%) out of 66 cases.
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Prophylaxis effect and clinical therapy of combination use of cefotiam (CTM), sulbenicillin (SBPC) and cefsulodin (CFS) have been investigated, and the results were as follows. 1. Prophylaxis effect of CTM-SBPC combination therapy was very useful. CTM-SBPC combination therapy was performed to patients who are at high risk for infectious complications. Prophylaxis effect of CTM-SBPC was judged by fever over 38 degrees C, and was better than usual antibiotic treatment. 2. CTM-SPBC-CFS combination therapy was performed against severe infections during early remission, and the overall effectiveness rate was 83.3% (5/6). 3. No remarkable side effect was observed in this investigation.
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A series of morphological studies at the light- and electron-microscopical levels have led to the conclusion that a significant proportion of immature thymic lymphocytes would undergo asymmetric (differential) cell division: at the telophase of such dividing cells one of two daughter cells retains morphological characteristics very similar to those of its parent cell, whereas the counterpart daughter cell looks much differentiated. Polarization of the cytoplasm is considered to be the mechanism whereby the asymmetry is generated. The phenomenon is observable in both prenatal and postnatal life of all the animal species examined, that is chicken, guinea pig, rat and mouse, meaning that it is a general phenomenon among the animals possessing the thymus. The frequency of asymmetrically-dividing cells is relatively high in large-sized cell fractions (more than 40% of total dividing cells). The conclusions leading from the above-mentioned morphological studies are supported by the cell kinetic studies performed by Metcalf in the mid-1960s. Asymmetric cell division may be primarily involved in the maintenance of immature 'stem cells' while concomitantly producing differentiated cells of smaller sizes. The phenomenon might also play an important role in the differentiation of thymocytes into distinct lineage of thymic lymphocytes such as cortex and medulla lymphocytes. Furthermore, the present findings raises the idea that hematopoietic cells including lymphoid cells would possess, in general, intracellular mechanisms to undergo asymmetric cell division.
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T4 deiodination has been studied using the fetal liver and kidney of miniature goats during late pregnancy. They were homogenized in 0.15M phosphate buffer, pH7.4 to make 25% homogenate which was incubated with outer ring labeled 125I-T4, 125I-rT3 or inner ring labeled 125I-T4 at 37 degrees C for 15, 30, 60, 90 or 120 min. either with or without 20mM DTT. After incubation they were extracted in ethanol and subjected to TLC to separate 125I-compounds. The radioactivity of each compound was measured and its production rate was expressed as a percentage of total radioactivity of the extract. The activity of deiodination in the outer ring of T4 in fetal liver was similar to that observed in the mother's . Fetal kidney showed a markedly increased activity in deiodination in the inner ring compared to that of all the other tissues studied. In contrast it showed the lowest deiodination in the outer ring. The addition of 20mM DTT had little effect on T4 deiodination in fetal organs. These data indicate a significant difference between T4 deiodinating activity in the liver and kidney of the fetus. It is felt that the kidney plays a more important role in T4 deiodination than the liver during fetal life.
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The effect of highly purified leukocytosis (lymphocytosis)-promoting factor (LPF) of Bordetella pertussis on physical lymphocyte and reticuloepithelial (RE) cell association was studied in an in vitro thymus model. First, a simplified in vitro system to assess the lympho-RE-cell association was developed. A completely confluent layer of thymic RE cells was formed by cultivating trypsinized thymus cell suspensions from 2- to 7-day-old mice. When thymic lymphoid cells were seeded on this cell layer and cultivated overnight, a significant proportion of them were found underneath the RE cell layer. This physical lympho-RE-cell association was quantitated by counting the lymphoid cells underneath the RE cell layers. Second, the effect of LPF on this physical lympho-RE-cell association phenomenon was investigated. Addition of LPF to the culture markedly inhibited the formation of the lympho-RE-cell complex; that is, it inhibited the infiltration of lymphoid cells under the RE cell layer. LPF rendered a nearly maximal level of inhibitory effect at a dose of 0.1 ng/ml. Furthermore, LPF enhanced the liberation of lymphoid cells from preformed lympho-RE-cell complexes. On the other hand, LPF had no direct cytotoxic effect on lymphoid cells at doses below 1 microgram/ml. In order to investigate whether LPF produced the effect by acting on lymphoid cells, RE cells, or both, the following experiments were performed. When lymphoid cells were pretreated with LPF and added to normal RE cell layers, the lympho-RE-cell association was maximally inhibited above the dose of 1 ng/ml. Treatment of these LPF-treated lymphoid cells with anti-LPF antibodies failed to abrogate the effect of LPF. When RE cell layers were similarly pretreated with LPF and were cultivated with normal lymphoid cells, however, much higher doses of LPF, above 100 ng/ml, were required for maximal inhibition. Furthermore, treatment of these LPF-treated RE cells with anti-LPF antibodies abrogated the effect of LPF. Therefore, the apparent effect of LPF on RE cells was considered to be due to the carry-over by RE cells of LPF, which should directly act on lymphoid cells at extremely low doses. On the basis of these results, it was concluded that LPF acted directly on lymphoid cells without mediation of RE cells. These in vitro results appear to parallel the effects of LPF in vivo, where it induces a depletion of cells in the thymus. The model may be useful to study this phenomenon and the concomitant accumulation of blood lymphocytes.
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