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Biomedical subjects

M Song

Publications and source records attributed to M Song.

At least 91 records · Page 5Linked to original sources
PubMed1999

[Comparative study of HLA-DRB1 allele in patients with chronic bronchitis and bronchial asthma].

OBJECTIVE: To make a comparative study of HLA-DRB1 allele frequencies in the cases of chronic bronchitis and bronchial asthma. METHODS: The authors investigated 74 patients with chronic bronchitis and 64 patients with bronchial asthma from among the Han people in Shanxi province. PCR/SSP technique was used for HLA-DRB1 typing, and the patients' data were compared with the normal controls'. RESULTS: The frequency of HLA-DRB1*1201/1202(24.32%) was significantly increased in the chronic bronchitis group(P<0.01),and the frequency of HLA-DRB1*1501/1502(23.44%) was significantly increased in the bronchial asthma group(P<0.05). The frequencies of other DRB1 alleles were not significantly increased in these groups. CONCLUSION: The results indicate that HLA-DRB1*1201/1202 allele is associated with chronic bronchitis and HLA-DRB1*1501/1502 allele is associated with bronchial asthma in the patients among the Hans in Shanxi province.

Alleles↗
PubMed1999

[Transcatheter arterial chemoembolization combined with external radiation for primary liver cancer].

OBJECTIVE: To evaluate the therapeutic efficacy of transcatheter arterial chemoembolization (TACE) combined with external radiotherapy (RT) for primary liver cancer. METHODS: The effectiveness of combined therapy with TACE and RT (76 cases) and TACE therapy alone (68 cases) was prospectively nonrandomly studied. RESULTS: In TACE + RT group, the response rate (CR + PR) was 53.9%, and the 1-, 2-, 3-year survival rate was 71.6%, 49.0%, and 44.5%, respectively. In contrast, the response rate of TACE alone was 33.8%, and the 1-, 2-, 3-year survival rate was 51.8%, 17.3%, and 17.3%, respectively. The difference between these two groups was statistically significant (P < 0.05). Factors of prognostic significance in the TACE + RT group were tumor type, tumor cell thrombi in the portal system, number of TACE treatment and the use of gelform in embolization. CONCLUSION: TACE + RT is more effective than TACE therapy alone. Understanding of the prognostic factors is useful for selection and management of patients.

Adult↗
PubMed1999

[A study on the effect of house dust mite on interleukin-10 secretion of peripheral blood lymphocytes in patients with allergic asthma].

OBJECTIVE: To investigate the relationship between interleukin (IL)-10 and allergic asthma, and the effect of antigen on IL-10. METHODS: The supernatant of cultured peripheral blood lymphocytes (PBLC) from 20 patients with allergic asthma and 20 normal subjects with different stimuli [home-dust-mite (HDM) or dexamethasone] was determined for IL-10, by means f enzyme-linked immunosorbent assay. RESULTS: (1) IL-10 was significantly decreased in asthma group, as compared with the control group (P < 0.01); (2) Less IL-10 was released in HDM-stimulated PBLC in asthma (P < 0.01); (3) IL-10 was obviously increased in dexamethason-stimulated whether in the asthma or in the control group. CONCLUSION: The diminished IL-10 production from PBLC could be related to asthmatic attack in patients with allergic asthma. Dexamethason might exert, at least, a part of anti-inflammatory activity by inducing IL-10 secretion of PBLC.

Adolescent↗
PubMed1999

[Changes of potassium and sodium content in erythrocyte of rabbit cardiac blood after death and timing of death].

We studied the changes of potassium and sodium contents in erythrocytes and the activity of erythrocyte membrane Na(+)-K(+)-ATPase in 40 cardiac blood samples of rabbit corpses at different postmortem intervals (PMI). The erythrocyte potassium content (RBCK) decreased linearly as the PMI increased (R = -0.829, P < 0.025). during postmortem 48 hours. The erythrocyte potassium content and the time after death had significant correlation. The activity of erythrocyte membrane Na(+)-K(+)-ATPase had no significant changes over postmortem 48 hours.

Animals↗
PubMed1998

IL-15 enhances the response of human gamma delta T cells to nonpeptide [correction of nonpetide] microbial antigens.

Human gamma delta T cells have the ability to rapidly expand and produce IFN-gamma in response to nonpeptide Ags of microbial pathogens, in particular a class of compounds known as the prenyl phosphates. We investigated the ability of IL-15, a T cell growth factor, to modulate prenyl phosphate-induced gamma delta T cell proliferation and cytokine production. IL-15 significantly enhanced the expansion of gamma delta T cells in the peripheral blood after stimulation in vitro with isopentenyl pyrophosphate. Moreover, using gamma delta T cell clones, we determined that IL-15-induced T cell proliferation was dependent on the IL-2R beta chain but not the IL-2R alpha chain. We therefore studied the IL-15R alpha chain expression in human gamma delta T cells in the presence or absence of nonpeptide Ags. We found IL-15R alpha mRNA expression in IL-15-stimulated and Ag-stimulated human gamma delta T cells but not in resting gamma delta T cells. Although IL-15 itself had little effect on the production of IFN-gamma, IL-15 plus IL-12 acted synergistically to augment IFN-gamma production by gamma delta T cells. Moreover, we showed that this increase in IFN-gamma could be explained by the dual activation of STAT1 and STAT4 by IL-15 and IL-12, respectively. Taken together, these results suggest that IL-15 may contribute to activation of human gamma delta T cells in the immune response to microbial pathogens.

Antigens, Bacterial↗
PubMed1998

Bilateral total hip arthroplasty comparing hydroxyapatite coating to porous-coated fixation.

Fifteen patients had bilateral hip replacement with a porous-coated hip replacement in one hip and in the contralateral hip the same design, which had adjunctive hydroxyapatite coating on the porous coating. These patients were followed for an average of 6.5 years (range, 5-7.9 years). There was no difference in the clinical Harris Hip Scores between the two hips in these patients. The radiographic measurements did show improved bone remodeling in hips with adjunctive hydroxyapatite coating as measured by proximal cancellous hypertrophy and evidence of a buttress sign. The fixation of stems with hydroxyapatite coating showed fewer radiolucent lines (P = .013). The improved bone remodeling, which we had previously reported, has been maintained in these hips at intermediate-term follow-up and the fixation of the hydroxyapatite-coated stems is also now better. The results in these patients with bilateral hips, which allows complete control of the bone type, immunology, weight, activity, and age of the patient, suggests that the use of hydroxyapatite coating does provide improved fixation and the possibility of improved durability.

Adult↗
PubMed1998

Limitations of standardized skin surface biopsy in measurement of the density of Demodex folliculorum. A case report.

Standardized skin surface biopsy (SSSB) is an appropriate method for measuring the density of Demodex folliculorum (Dd). Nevertheless, the observation of a clinical case demonstrates that, contrary to what was presumed before, a single SSSB can fail to collect the complete biotope of the mite. This could give rise to false negative results regarding the measurement of Dd.

Aged↗
PubMed1998

Occurrence of gouty tophi following acitretin therapy.

Acitretin (Ro 10-1670 or Neotigason), a free acid and the main metabolite of etretinate (Ro 10-9359 or Tigason), is the most recent of the retinoids used orally in the treatment of psoriasis and numerous other dermatoses exhibiting disorders of keratinization. In the majority of cases, its side-effects are similar to those of hypervitaminosis A.

Acitretin↗
PubMed1998

Filamentous actin disruption and diminished inositol phosphate response in gingival fibroblasts caused by Treponema denticola.

Previous reports have shown that Treponema denticola causes rearrangement of filamentous actin (F-actin) in human gingival fibroblasts (HGF). The purpose of this investigation was to determine the effect of T. denticola on the generation of inositol phosphates (IPs) in relation to a time course for F-actin disruption in HGF. Cultured HGF were exposed to washed cells of T. denticola ATCC 35405 for 140 min. Changes in the fluorescence intensity of rhodamine-phalloidin-labeled F-actin in serial optical sections of single HGF were quantified by confocal microscopy image analysis. The percentage of cells with stress fiber disruption was also determined by fluorescence microscopy. Challenge with T. denticola caused a significant reduction in F-actin within the first hour, especially at the expense of F-actin in the ventral third of the cells, and a significant increase in the percentage of HGF with altered stress fiber patterns. Significant concentration-dependent disruption of stress fibers was also caused by HGF exposure to a Triton X-100 extract of T. denticola outer membrane (OM). IPs were measured by a radiotracer assay based on the incorporation of myo-[3H]inositol into IPs in HGF incubated with LiCl to inhibit endogenous phosphatases. HGF challenge with several strains of T. denticola and the OM extract of T. denticola ATCC 35405 resulted in a diminished accumulation of radiolabeled IPs relative to both 15 and 1% fetal bovine serum, which served as strongly positive and background control agonists, respectively. The significantly diminished IP response to T. denticola ATCC 35405 occurred within 60 min, concomitant with significant reduction of total F-actin and disruption of stress fibers. Pretreatment with the proteinase inhibitor phenylmethylsulfonyl fluoride, which had previously been found to block T. denticola's degradation of endogenous fibronectin and detachment of HGF from the extracellular matrix, had little effect on F-actin stress fiber disruption and the IP response. Therefore, in addition to its major surface chymotrypsin-like properties, T. denticola expresses cytopathogenic activities that diminish the generation of IPs during the time course associated with significant cytoskeletal disruption in fibroblasts.

Actins↗
PubMed1998

Immunolocalization of gastrin-dependent histidine decarboxylase activity in rat gastric mucosa during feeding.

The localization of histidine decarboxylase (HDC) activity in the enterochromaffin-like (ECL) cells of the oxyntic mucosa was studied during fasting and refeeding using monoclonal (CURE no. 44178) and polyclonal (CURE no. 94211) antibodies directed against the COOH terminus of HDC (HDC-CT). Changes in HDC immunostaining were correlated with mucosal HDC enzyme activity. Immunoneutralization of circulating gastrin and atropine treatment during refeeding were used to determine the relative importance of gastrin and cholinergic mechanisms in the regulation of HDC activity and immunostaining. Fasting caused a rapid reduction in the number of ECL cells immunostaining for HDC that was correlated with an almost complete loss of mucosal HDC enzyme activity. Refeeding restored both HDC immunostaining and enzyme activity within 2-4 h, and this response was inhibited by gastrin immunoneutralization but not by atropine treatment. Immunostaining was uniformly decreased and restored in the lower half of the oxyntic mucosa, which corresponds to the predominant area of ECL cells in the gastric gland. Histamine immunostaining and mucosal histamine content were not significantly changed during fasting and refeeding or by gastrin antibody and/or atropine treatment during refeeding. These findings indicate that HDC activity correlates with HDC-CT immunostaining and that both HDC activity and HDC-CT immunostaining are regulated by gastrin during refeeding.

Amino Acid Sequence↗
PubMed1998

Acne neonatorum and familial hyperandrogenism.

We report a case of acne neonatorum developed in a boy since birth. His mother and his aunt had different signs of hyperandrogenism including acne and hirsutism. This case illustrates the correlation between acne neonatorum and familial hyperandrogenism. It shows the importance of the family history and the eventual clinical and serological presence of hyperandrogenism in the mother when confronted with acne neonatorum.

Acne Vulgaris↗
PubMed1998

Cellular composition and anatomic distribution in nonfunctioning pancreatic endocrine tumors: immunohistochemical study of 30 cases.

OBJECTIVE: To investigate the cytological pattern and distribution in nonfunctioning pancreatic endocrine tumors. METHODS: Using labeled streptavidin-biotin (LSAB), immunohistochemical staining for insulin, glucagon, somatostatin, pancreatic polypeptide and gastrin was performed on 30 nonfunctioning pancreatic endocrine tumors from 30 patients. The cellular composition and anatomic distribution in these tumors were analyzed. RESULTS: Of 30 tumor tissues, 22 (73.3%) were found to contain cells immunoreactive to 1-4 kinds of peptide hormones; 17 (56.7%) showed positive staining for more than one peptide and up to 4 peptides; and 8 (26.7%) showed negative immunoreaction to all antiserum applied. No tumor was found to contain immunoreactive gastrin. Among 17 multihormonal tumors, 4 contained 2 kinds of peptide hormones, 8 had 3 kinds, and 5 harbored 4 kinds of peptide hormones. In addition, the difference in the number and type of positive endocrine cells between the tumors arising from the head of the pancreas and those arising from the body and tail of the pancreas were statistically significant (P < 0.05). CONCLUSIONS: Immunohistochemically, the high positive rate to peptide hormones suggests that the nonfunctioning pancreatic endocrine tumors are actually not nonfunctioning; they are asymptomatic pancreatic endocrine tumors. Moreover, an uneven distribution of positive endocrine cells in the nonfunctioning pancreas endocrine tumors within the pancreas was identified.

Adenoma, Islet Cell↗
PubMed1998

[Expression of surfactant protein SP-A, SP-B, and SP-C mRNA in lungs of rats with bleomycin-induced pulmonary fibrosis].

OBJECTIVE: The expressions of surfactant protein (SP)SP-A, SP-B, and SP-CmRNA in lungs of rats with bleomycin-induced pulmonary fibrosis were studied. METHOD: A single dose of bleomycin(BLM) was intratracheal injected to induce pulmonary fibrosis of rats. Animals were killed at day 3, 7, 14 and 28 after BLM administration. The total RNA was extracted from the lung tissue. The expressions of SPsmRNA were analyzed with Northern blot. RESULT: The number of alveolar type II epithelial cells increased in BLM-administered rats. The expressions of SP-A, SP-B and SP-CmRNA decreased at day 3 after BLM administration and decreased maximally at day 7, and then began to increase at day 14 and significantly increased at day 28, though they were still below the control levels. CONCLUSION: The results show that the changes of expressions of SP-A, SP-B and SP-CmRNA occur during the development of bleomycin-induced pulmonary fibrosis in rats and they may play a role in the pathogenesis of lung fibrosis.

Animals↗
PubMed1998

[Expressions of TNF alpha, PDGF in alveolar type II epithelial cells of rats with bleomycin-induced pulmonary fibrosis].

OBJECTIVE: The expressions of TNF alpha and PDGF in alveolar type II epithelial cells of rats with bleomycin(BLM)-induced pulmonary fibrosis were studied. METHOD: A single intratracheal injection of BLM was administrated to induce pulmonary fibrosis of rats. Animals were killed at day 3,7,14 and 28 after BLM-administration. The immunohistochemical methods were used to analyze the expressions of TNF alpha and PDGF proteins in alveolar epithelium of rats. The total RNA was extracted from the alveolar type II epithelial cells of rats and the expressions of TNF alpha and PDGF mRNA were analyzed with Northern blot. RESULT: TNF alpha and PDGF were expressed in the alveolar type II epithelial cells of BLM-administrated rats. The expression of TNF alpha elevated in median and late-stage of the process and reached the peak at day 28. While the expression of PDGF elevated in early-stage and reached the peak at day 7. By contrast, TNF alpha and PDGF weren't expressed in the alveolar type II epithelial cells of normal controls. CONCLUSION: The results show that the alveolar type II epithelial cell from rats with pulmonary fibrosis overexpresses TNF alpha and PDGF and they may play roles in the pathogenesis of lung fibrosis.

Animals↗
PubMed1997

Large conductance voltage- and calcium-dependent K+ channel, a distinct member of voltage-dependent ion channels with seven N-terminal transmembrane segments (S0-S6), an extracellular N terminus, and an intracellular (S9-S10) C terminus.

Large conductance voltage- and Ca2+-dependent K+ (MaxiK) channels show sequence similarities to voltage-gated ion channels. They have a homologous S1-S6 region, but are unique at the N and C termini. At the C terminus, MaxiK channels have four additional hydrophobic regions (S7-S10) of unknown topology. At the N terminus, we have recently proposed a new model where MaxiK channels have an additional transmembrane region (S0) that confers beta subunit regulation. Using transient expression of epitope tagged MaxiK channels, in vitro translation, functional, and "in vivo" reconstitution assays, we now show that MaxiK channels have seven transmembrane segments (S0-S6) at the N terminus and a S1-S6 region that folds in a similar way as in voltage-gated ion channels. Further, our results indicate that hydrophobic segments S9-S10 in the C terminus are cytoplasmic and unequivocally demonstrate that S0 forms an additional transmembrane segment leading to an exoplasmic N terminus.

Amino Acid Sequence↗
PubMed1997

Pancreatic acinar cells produce, release, and respond to tumor necrosis factor-alpha. Role in regulating cell death and pancreatitis.

The aim of this study was to determine whether tumor necrosis factor-alpha (TNFalpha) and receptors for TNFalpha are expressed in the exocrine pancreas, and whether pancreatic acinar cells release and respond to TNFalpha. Reverse transcription PCR, immunoprecipitation, and Western blot analysis demonstrated the presence of TNFalpha and 55- and 75-kD TNFalpha receptors in pancreas from control rats, rats with experimental pancreatitis induced by supramaximal doses of cerulein, and in isolated pancreatic acini. Immunohistochemistry showed TNFalpha presence in pancreatic acinar cells. ELISA and bioassay measurements of TNFalpha indicated its release from pancreatic acinar cells during incubation in primary culture. Acinar cells responded to TNFalpha. TNFalpha potentiated NF-kappaB translocation into the nucleus and stimulated apoptosis in isolated acini while not affecting LDH release. In vivo studies demonstrated that neutralization of TNFalpha with an antibody produced a mild improvement in the parameters of cerulein-induced pancreatitis. However, TNFalpha neutralization greatly inhibited apoptosis in a modification of the cerulein model of pancreatitis which is associated with a high percentage of apoptotic cell death. The results indicate that pancreatic acinar cells produce, release, and respond to TNFalpha. This cytokine regulates apoptosis in both isolated pancreatic acini and experimental pancreatitis.

Animals↗