Search PubMed⌕ Search

Biomedical subjects

M Soller

Publications and source records attributed to M Soller.

At least 73 records · Page 4Linked to original sources
PubMed1993

Milk as a source of deoxyribonucleic acid and as a substrate for the polymerase chain reaction.

Somatic cells in milk were used as a source of DNA and as a substrate for the polymerase chain reaction. Successful DNA extraction required a minimum total of 17 x 10(6) cells in the milk sample. The DNA yield per cell was highly variable but comparable, on average, with the yield from peripheral blood leukocytes. In all cases, direct polymerase chain reaction on milk samples, using a variety of primer pairs, yielded amplified products of correct size that were identical to those obtained by polymerase chain reaction of purified DNA extracted from milk or blood. Milk and DNA extracted from milk served as substrates for direct sequencing of part of the bovine growth hormone gene. Milk samples with added preservative were stored over 200 d without effect on the polymerase chain reaction. Thus, milk can often substitute for blood as a source of DNA for Southern blot analysis and is a preferred substrate for the polymerase chain reaction. The technical convenience of milk as a source of DNA can be expected to increase the field of application of marker-based methods for genetic analysis and genetic improvement of economic traits in dairy cattle.

Animals↗
PubMed1991

Distribution of endogenous viruses in some commercial chicken layer populations.

The distribution of endogenous virus (ev) genes was studied in five commercial layer lines; four were brown-egg types and one was White Leghorn. The DNA samples were obtained from nine birds of each line and digested with SacI and BamHI endonucleases. The DNA fragments were separated by gel electrophoresis, and Southern blots were prepared and examined for the presence of ev genes following hybridization with the labeled recombinant plasmid pRAV-2 and autoradiography. Almost all fragments were present in more than one line, suggesting that each line has drawn a random assortment of ev genes from the same common pool, possibly as a founder effect. A great degree of polymorphism is shown by the ev genes in the lines investigated. Most fragments were present at low or intermediate frequencies. Very few fragments were present in only one line or in only one bird. This suggests that de novo integration of ev genes at new sites is a relatively rare event. Very few fragments were present at high frequencies, and none of the fragments, except for the BamHI internal fragments, were present in all birds of all lines. Considering that ev genes are present in the wild progenitor of the domestic chicken, the lack of fixation of any ev genes in the chicken genome is remarkable. It is proposed that ev genes have general deleterious effects in the chicken (hence, the lack of fixation), but those ev genes that have been retained in the chicken genome have favorable effects under some circumstances with consequent equilibrium at low to intermediate frequencies. It is speculated that the retained ev genes may represent sites of favorable mutation via insertional mutagenesis and, hence, a potential route to the cloning of genes of economic importance in poultry.

Analysis of Variance↗
PubMed1991

Selection for high and low threshold body weight at first egg in broiler strain females. 1. Direct response to selection and correlated effects on juvenile growth rate and age at first egg.

A selection program for high and low threshold body weight at first egg was carried out in a broiler line. Selection was on the basis of weight at first egg, following gradual release from feed restriction at a relatively advanced age. After six generations of selection, the lines differed by 862 g in the trait under selection. In addition, 6-wk body weight of high-line (HL) birds was 91 g greater, and age at first egg was 32 days greater than in low-line (LL) birds. When raised under an ad libitum feeding regimen from hatch, HL birds entered lay 20 days later than LL birds. When onset of lay was markedly delayed by maintaining feed restriction until 29 wk of age, body weight at first egg of HL birds was greater by 645 g than that of LL birds. Similarly, when birds of the two lines were subjected to forced molt and brought to a body weight well below that of initial body weight at first egg, and then allowed to gain weight and reenter lay, body weight difference at first egg of HL and LL birds following rehabilitation was similar to that found on original entry into lay. It is proposed that the results may most plausibly be explained as resulting from a primary effect of the selection procedure on the time required to first egg from onset of sexual competence, defined as onset of responsiveness to lay-inducing factors such as light. Alternative explanations involve effects of the selection procedure on threshold weight or threshold age requirements for sexual competence.

Animal Feed↗
PubMed1991

Two-way selection for threshold body weight at first egg in broiler strain females. 2. Effect of supplemental light on weight and age at first egg.

In order to undergo the processes leading to lay, female chickens must reach a minimum threshold age and body weight, and be exposed to some minimum amount of light stimulation. By two-way selection over six generations lines were developed that differed markedly in body weight at first egg. The effect of supplemental light on age and body weight at first egg of these lines was studied in two experiments. In the first experiment, birds of the two lines were provided ad libitum access to feed from hatch, reaching the weight threshold at an early age. Supplemental light was provided at either 91 or at 126 days of age (before or after the age threshold, respectively). Differences in body weight and age at first egg of the two lines were the same in both light treatments. In the second experiment, birds of the two lines were raised under feed restriction to 154 days of age and then released to ad libitum feeding under supplemental or natural (autumn) light. Differences between the lines in age and weight at first egg were greater in the group maintained under natural light than under supplemental light. The results are interpreted in terms of a model relating age at first egg to weight and age thresholds for onset of competence to respond to light stimulation, and to cumulative light stimulation required from onset of competence to first egg. It is proposed that selection affected the total amount of light stimulation required from onset of competence to onset of lay, but did not affect weight or age thresholds for onset of competence.

Aging↗
PubMed1990

Large restriction fragments containing poly-TG are highly polymorphic in a variety of vertebrates.

Southern blots of genomic DNA from a variety of species digested by restriction endonucleases having a four-bp specificity, were probed with a bovine genomic clone consisting of seven tandem poly-TG stretches separated by a 29bp linker sequence. Highly variable DNA 'fingerprint' patterns were obtained in chicken, sheep, and horse, moderately variable DNA 'fingerprints' in mouse and man, and a monomorphic pattern in Drosophila. In chicken, horse and man a (TG)10 synthetic oligonucleotide probe gave results identical to those given by the bovine probe. Furthermore, in chicken the DNA fingerprint variation showed typical Mendelian inheritance and differed from the fingerprints obtained with Jeffreys 33.6 and M13 minisatellite probes. Thus, for a variety of vertebrate species, poly-TG-containing probes can uncover useful genetic variation.

Animals↗
PubMed1990

(TG)n uncovers a sex-specific hybridization pattern in cattle.

Screening of a bovine genomic library with the human minisatellite 33.6 probe uncovered a family of clones that, when used to probe Southern blots of bovine genomic DNA digested with the restriction enzyme HaeIII or MboI, revealed sexually dimorphic, but otherwise virtually monomorphic, patterns among the larger DNA fragments to which they hybridized. Characterization of one of these clones revealed that it contains different minisatellite sequences. The sexual dimorphism hybridization pattern observed with this clone was found to be due to multiple copies of two tandemly interspersed repeats: the simple sequence (TG)n and a previously undescribed 29-bp sequence. Both repeats appear to share many genomic loci including autosomal loci. In contrast, Southern analysis of AluI- or HinfI-digested bovine DNA with the (TG)n repeat used as a probe yielded substantial polymorphism. These results show that (i) different minisatellites can be found in a cluster, (ii) both simple and more complex repeated sequences other than the simple quaternary (GATA)n repeat can be sexually dimorphic, and (iii) simple repeats can reveal substantial polymorphism.

Animals↗
PubMed1990

Toward a unified approach to genetic mapping of eukaryotes based on sequence tagged microsatellite sites.

The genomes of all eukaryotes appear to contain a special class of loci, termed microsatellites, which can serve, if sequenced and taken as the substrate for the polymerase chain reaction, as highly informative, locus-specific markers. By analogy to the "sequence tagged sites" recently proposed by Olsen et al. for standardizing the human physical gene map, these microsatellite markers are termed "sequence tagged microsatellite sites" (STMS). Genetic maps based on STMS will share with the Olsen physical maps the advantage that mapping vocabularies will be standardized to the DNA sequence base and that access to any particular locus will not require shipping or storing cloned probes. The species map will consist simply of a listing of nucleotide sequences. Reference populations for developing STMS maps can be chosen on the basis of biological or economic interest. It will not be necessary to maximize for genetic divergence.

Animals↗
PubMed1990

Power of daughter and granddaughter designs for determining linkage between marker loci and quantitative trait loci in dairy cattle.

There is considerable interest in bovine DNA-level polymorphic marker loci as a means of mapping quantitative trait loci (QTL) of economic importance in cattle. Progeny of a sire heterozygous for both a marker locus and a linked QTL, which inherit different alleles for the marker, will have different trait means. Based on this, power to detect QTL, as a function of QTL effect, heritability of the trait, and number of animals tested was determined for 1) daughter design, marker genotype and quantitative trait values assessed on daughters of sires heterozygous for the markers; and 2) granddaughter design, a newly devised alternative design in which marker genotype is determined on sons of heterozygous sires and quantitative trait value measured on daughters of the sons. For equal numbers of assays, power increased with the number of daughters per sire (design 1) and sons per grandsire (design 2). For equal power and heritability less than or equal to .2, design 2 required half as many marker assays as design 1, e.g., with heritability of .2, QTL effect of .2 SD units, and type 1 error of .01, power was .70 if 400 daughters of each of 10 sires were assayed for the markers and .95 if markers were assayed on 100 sons of each of 20 sires with 50 granddaughters per son.

Alleles↗
PubMed1989

The bovine gene map.

The present status of the bovine gene map as well as some of the methods and strategies important for future efforts in completing the gene map of cattle are reviewed.

Animals↗
PubMed1988

Mapping of bovine prolactin and rhodopsin genes in hybrid somatic cells.

The genes encoding bovine prolactin and rhodopsin were assigned to syntenic groups on the basis of hybridization of DNA from a panel of bovine-hamster hybrid somatic cell lines with cloned prolactin and rhodopsin gene probes. Prolactin was found to be syntenic with previously mapped glyoxalase, BoLA and 21-hydroxylase genes, establishing a syntenic conservation with human chromosome 6. The presence of bovine rhodopsin sequences among the various hybrid cell lines was not concordant with any gene previously assigned to one of the 23 defined autosomal syntenic groups. Thus, rhodopsin marks a new bovine syntenic group, U24, leaving only five cattle autosomes unmarked by at least one biochemical or molecular marker.

Animals↗
PubMed1988

Screening of Israeli Holstein-Friesian cattle for restriction fragment length polymorphisms using homologous and heterologous deoxyribonucleic acid probes.

Genomic DNA of Israeli Holstein-Friesian dairy cattle were screened with a battery of 17 cloned or subcloned DNA probes in an attempt to document restriction fragment length polymorphisms at a number of genetic loci. Restriction fragment length polymorphisms were observed at the chymosin, oxytocin-neurophysin I, lutropin beta, keratin III, keratin VI, keratin VII, prolactin, and dihydrofolate reductase loci. Use of certain genomic DNA fragments as probes produced hybridization patterns indicative of satellite DNA at the respective loci. Means for distinguishing hybridizations to coding sequences for unique genes from those to satellite DNA were developed. Results of this study are discussed in terms of strategy for the systematic development of large numbers of bovine genomic polymorphisms.

Animals↗