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M Soller

Publications and source records attributed to M Soller.

At least 55 records · Page 3Linked to original sources
PubMed1996

Selection for high and low threshold body weight at first egg in broiler strain females. 6. Threshold weight for onset of photosensitivity in females of the selection lines.

A two-way selection program, carried out for high and low threshold BW at onset of lay (TWL) resulted in a marked difference in TWL in the selected lines. Later studies showed that the selection procedure had decreased photoperiodic drive in the high line (HL) as compared to the low line (LL), in this way increasing the number of days required from onset of photosensitivity to onset of lay in the HL as compared to the LL. The present study was carried out to determine whether the difference in photoperiodic drive was sufficient to account for the entire difference in TWL between the HL and LL, or whether it is also necessary to postulate a difference in threshold weight for onset of photosensitivity (TWSM) as well. Birds of the two lines were raised under feed restriction to 22 wk reaching weights (1,881.8 g) for LL, 2,304.8 g for HL), well below anticipated TWSM. They were then provided gradually increasing amounts of feed, resulting in daily gains of 3 to 5 g for the LL and 8 to 9 g for the HL. Under this regimen, the HL entered lay an average of 64.4 d later and at a body weight 752.8 g greater than the LL. On the basis of previous studies of these lines, of the 64.4 d delay in onset of lay in the HL as compared to LL, 21 d can be attributed to the difference in photoperiodic drive. The excess of 43 d must thus represent a difference in TWSM. During this period the HL were gaining about 8.5 g/d, and at 22 wk HL body weight was already 153 g greater than LL body weight. In all then, TWSM of HL is estimated at 518 g greater than that of the LL. Thus, the selection procedure employed for TWL apparently affected TWSM as well as photoperiodic drive.

Animals↗
PubMed1996

Selection for high and low threshold body weight at first egg in broiler strain females. 7. Effect of photoperiod on body weight and age at onset of mature semen production, in males of the selection lines, and in commercial broiler and layer males.

Effect of photoperiod on age and BW at attainment of mature semen production was examined in males of lines selected for high (HL) and low (LL) threshold BW at onset of lay, and in males of a commercial Cornish male line (MC), a commercial broiler breeder female line cross (BX), and a commercial layer cross (LX). Photoperiod treatments were: I, increasing natural spring light; and D, steadily decreasing artificial light provided in a light-controlled room. Under I, age at onset of mature semen production was 168.5, 133.6, 138.1, 124.0, and 118.0 d for HL, LL, MC, BX, and LX males, respectively. Under D, age at onset of mature semen production increased by 18.3, 8.0, 55.9, 60.9, and -2.0 d, respectively. The differential effect of Treatment D on the various genetic stocks is attributed to differences between stocks in photoperiodic drive. Therefore, the very great increase in age at onset of mature semen production in BX and MC males points to a severe reduction in photoperiodic drive in males of these stocks, and to future problems in male fertility in broiler breeder flocks entering lay in the summer and fall. Lines HL, LL, BX, and LX rank the same with respect to photoperiodic drive, whether measured in males as in this experiment, or in females, as in a previous experiment. This result suggests that genetic factors affecting photoperiodic drive come to similar expression in males and females. Thus, selection for increased photoperiodic drive in broiler line males may be able to increase photoperiodic drive in broiler line females, and in this way improve female reproductive performance.

Animals↗
PubMed1995

Advanced intercross lines, an experimental population for fine genetic mapping.

An advanced intercrossed line (AIL) is an experimental population that can provide more accurate estimates of quantitative trait loci (QTL) map location than conventional mapping populations. An AIL is produced by randomly and sequentially intercrossing a population that initially originated from a cross between two inbred lines or some variant thereof. This provides increasing probability of recombination between any two loci. Consequently, the genetic length of the entire genome is stretched, providing increased mapping resolution. In this way, for example, with the same population size and QTL effect, a 95% confidence interval of QTL map location of 20 cM in the F2 is reduced fivefold after eight additional random mating generations (F10). Simulation results showed that to obtain the anticipated reduction in the confidence interval, breeding population size of the AIL in all generations should comprise an effective number of > or = 100 individuals. It is proposed that AILs derived from crosses between known inbred lines may be a useful resource for fine genetic mapping.

Animals↗
PubMed1995

Two-way selection for threshold body weight at first egg in broiler strain females. 5. Replication of results in a two-generation selection experiment.

Selection for high and low threshold weight for onset of lay (ThrWtLay), carried out in a broiler line over six generations, was previously found to increase reproductive performance in the low line (LL). Because of the potential importance of this effect, a two-generation replication of the original experiment was carried out using a modern broiler line. Selection was on the basis of ThrWtLay, measured as body weight at first egg, following gradual release from feed restriction. The total response to selection over two generations was 382 g, giving a realized heritability of .42. The difference in 6-wk body weight of the two lines was +19 g in favor (P > .05) of the high line (HL). Reproductive performance was measured in the S2 generation and in the first (R1) generation of relaxed selection. On the average, age at first egg of LL was 17.6 d earlier, and prepeak, postpeak, and total egg production of LL to a fixed age was 9.9, 1.1, and 11.1 eggs greater, respectively, than that of HL. The differences in age at first egg, prepeak, and total egg production were significant; those in postpeak production were not. There was a difference of 1.1 g in favor (P > .05) of HL for egg weight. Thus, the results of this short-term selection experiment are broadly consistent with those of the original, long-term selection. Namely, ThrWtLay responded strongly to selection, with a powerful accompanied response in age at onset of lay and in prepeak egg production, and only a small effect on 6-wk body weight. However, in contrast to the results obtained previously, LL in the present experiment showed only a small increase (P > .05) in postpeak production relative to HL. Nevertheless, although an effect on postpeak production was not obtained in the present experiment, the results do provide general support for the previously expressed view that selection for early onset of lay, on release from feed restriction, may provide a means of increasing photoperiodic drive and consequent reproductive performance of broiler breeders.

Animals↗
PubMed1994

How is it that microsatellites and random oligonucleotides uncover DNA fingerprint patterns?

Minisatellites, microsatellites, and short random oligonucleotides all uncover highly polymorphic DNA fingerprint patterns in Southern analysis of genomic DNA that has been digested with a restriction enzyme having a 4-bp specificity. The polymorphic nature of the fragments is attributed to tandem repeat number variation of embedded minisatellite sequences. This explains why DNA fingerprint fragments are uncovered by minisatellite probes, but does not explain how it is that they are also uncovered by microsatellite and random oligonucleotide probes. To clarify this phenomenon, we sequenced a large bovine genomic BamHI restriction fragment hybridizing to the Jeffreys 33.6 minisatellite probe and consisting of small and large Sau3A-resistant subfragments. The large Sau3A subfragment was found to have a complex architecture, consisting of two different minisatellites, flanked and separated by stretches of unique DNA. The three unique sequences were characterized by sequence simplicity, that is, a higher than chance occurrence of tandem or dispersed repetition of simple sequence motifs. This complex repetitive structure explains the absence of Sau3A restriction sites in the large Sau3A subfragment, yet provides this subfragment with the ability to hybridize to a variety of probe sequences. It is proposed that a large class of interspersed tracts sharing this complex yet simplified sequence structure is found in the genome. Each such tract would have a broad ability to hybridize to a variety of probes, yet would exhibit a dearth of restriction sites. For each restriction enzyme having 4-bp specificity, a subclass of such tracts, completely lacking the corresponding restriction sites, will be present. On digestion with the given restriction enzyme, each such tract would form a large fragment.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1994

Selective DNA pooling for determination of linkage between a molecular marker and a quantitative trait locus.

Selective genotyping is a method to reduce costs in marker-quantitative trait locus (QTL) linkage determination by genotyping only those individuals with extreme, and hence most informative, quantitative trait values. The DNA pooling strategy (termed: "selective DNA pooling") takes this one step further by pooling DNA from the selected individuals at each of the two phenotypic extremes, and basing the test for linkage on marker allele frequencies as estimated from the pooled samples only. This can reduce genotyping costs of marker-QTL linkage determination by up to two orders of magnitude. Theoretical analysis of selective DNA pooling shows that for experiments involving backcross, F2 and half-sib designs, the power of selective DNA pooling for detecting genes with large effect, can be the same as that obtained by individual selective genotyping. Power for detecting genes with small effect, however, was found to decrease strongly with increase in the technical error of estimating allele frequencies in the pooled samples. The effect of technical error, however, can be markedly reduced by replication of technical procedures. It is also shown that a proportion selected of 0.1 at each tail will be appropriate for a wide range of experimental conditions.

Chromosome Mapping↗
PubMed1994

Determining relative microsatellite allele frequencies in pooled DNA samples.

Accurate quantification of relative allele frequencies in pooled DNA samples can be carried out for microsatellite markers having a dinucleotide repeat unit, conditional on the absence of overlapping "shadow" bands. This provides a basis for extending DNA pooling to this useful class of DNA marker. Expressions for the standard error of densitometric estimates of allele frequencies from pooled samples are presented, and their statistical application is illustrated in a variety of situations. This enables DNA pooling to be utilized in situations requiring the testing of statistical hypotheses concerning differences in allele frequencies between populations, or samples.

Alleles↗
PubMed1994

Selection for high and low threshold body weight at first egg in broiler strain females. 4. Photoperiodic drive in the selection lines and in commercial layers and broiler breeders.

A two-way selection program, carried out for high and low threshold BW at onset of lay (ThrWtLay), decreased photoperiodic drive of the high line (HL) relative to the low line (LL). Photoperiodic drive in modern broiler breeder females was compared to layer females in experiments using the HL and LL lines, a commercial layer cross (LX), and a commercial broiler breeder female line cross (BX). Treatment groups were: NS, natural decreasing light to 20 wk, followed by natural light supplemented to 16 h daily with artificial light; NN, natural decreasing light to end of experiment; DD, dark room from hatch to end of experiment; ND, natural decreasing light to 20 wk, dark room thereafter. Under NS, mean age and BW at first egg was greater for the HL line than for the LL line. The difference increased by a further 8.9 d and 217.5 g under NN. Similarly, under NS, mean age and BW at first egg was greater for BX than for LX, and the difference increased by a further 22.6 d and 256.1 g under NN. These interactions indicate greater photoperiodic drive of LX and LL stocks relative to the HL and BX stocks. Prepeak and peak egg production (EP) was greatest for the LX, lower for the LL, and lower yet for HL and BX. Photoperiodic drive may be of major importance in determining prepeak and peak EP. Except for the DD treatment, age and BW of the LX cross at first egg were virtually identical across treatments, those of the LL line were similar, whereas those of the HL and BX stocks showed differences. Similarly, EP curves of the LX cross were virtually identical across treatments, those of the LL line were relatively uniform, but those of the HL and BX stocks again showed great differences. Standard deviation and coefficient of variation of peak EP were very low for the LX cross, still low, but significantly greater for the LL line, and significantly greater yet again for both HL and BX stocks. Thus, increased photoperiodic drive may also be associated with greater stability of reproductive performance. Selection for early onset of lay on release from feed restriction may provide a means of increasing photoperiodic drive and consequent reproductive performance of broiler breeders.

Age Factors↗
PubMed1994

Nonlinear effects of chicken endogenous viruses on body weight may be responsible for maintaining these elements in a stable genetic polymorphism.

The effects of presence or absence of individual endogenous virus (ev) genes on production traits was studied in a highly productive commercial layer cross. Age and BW at first egg, egg production, egg weight, and mature BW were recorded for each bird. The birds were examined for presence of ev gene fragments by Southern analysis. A general linear model was used to determine significance of effects of the 21 individual ev fragments on the individual traits and the effects of all ev fragments taken together on each of the traits. Seven significant effects were found for individual ev fragments on individual traits. Four of these involved BW at first egg, and all ev gene fragments taken together had a significant effect on BW at first egg, explaining 17% of total phenotypic variation in this trait. Significant nonlinear correlations were found between total number of ev genes and both BW at first egg and mature BW, with birds having a moderate number of ev genes showing the lowest BW. For age at first egg and egg weight, nonlinear correlations, although not significant, were consistent in sign with those found for BW, implying minimum trait magnitude at moderate number of ev genes. These effects imply that animals with intermediate numbers of ev genes will tend to be favored by commercial selection in layer flocks, whereas birds with either too many or too few ev genes will tend to be culled.(ABSTRACT TRUNCATED AT 250 WORDS)

Alpharetrovirus↗
PubMed1993

Detecting marker-QTL linkage and estimating QTL gene effect and map location using a saturated genetic map.

A simulation study was carried out on a backcross population in order to determine the effect of marker spacing, gene effect and population size on the power of marker-quantitative trait loci (QTL) linkage experiments and on the standard error of maximum likelihood estimates (MLE) of QTL gene effect and map location. Power of detecting a QTL was virtually the same for a marker spacing of 10 cM as for an infinite number of markers and was only slightly decreased for marker spacing of 20 or even 50 cM. The advantage of using interval mapping as compared to single-marker analysis was slight. "Resolving power" of a marker-QTL linkage experiment was defined as the 95% confidence interval for the QTL map location that would be obtained when scoring an infinite number of markers. It was found that reducing marker spacing below the resolving power did not add appreciably to narrowing the confidence interval. Thus, the 95% confidence interval with infinite markers sets the useful marker spacing for estimating QTL map location for a given population size and estimated gene effect.

Chromosome Mapping↗
PubMed1993

Sequence-tagged microsatellite sites as markers in chicken reference and resource populations.

Two chicken genomic libraries were screened for the presence of poly(TG/AC) microsatellite tracts. The number of positive clones was low, confirming the low frequency of such microsatellites in the chicken genome relative to mammalian genomes. Polymorphism of 29 microsatellite tracts, comprising 11 from the library screening and 18 obtained from GenBank, was examined in the East Lansing and Compton reference families, in a resource population formed by a cross between a single White Rock broiler and inbred Leghorn females, and in a panel of birds from five layer stocks. Twenty microsatellites, primarily of the poly(TG/AC) type, were polymorphic in at least one of the populations. Thirteen of the microsatellites were polymorphic in the East Lansing reference family and 13 were also polymorphic in the resource population, confirming that the genetic distance between White Rock and White Leghorn is about as great as between Jungle fowl and White Leghorn. Only six microsatellites were polymorphic in the Compton reference family, formed by a cross between two White Leghorn strains. Twelve of the microsatellites were mapped in the East Lansing and/or Compton reference families. These were well dispersed among the various linkage groups and did not show any indications of terminal clustering.

Animals↗