Biomedical subjects

M Shaw

Publications and source records attributed to M Shaw.

239 records · Page 14Linked to original sources

PubMedDate not supplied

Gallbladder density and iodine concentration in humans during oral cholecystography. A comparison of iopanoic acid and iopronic acid.

A comparison of two oral cholecystopaques, iopanoic acid (Telepaque) and iopronic acid (Oravue), was performed using normal volunteers. Using a double-blind crossover design, comparisons were made between the degree of gallbladder opacification and the amount of iodine recovered from the gallbladder. Bile was collected via a double lumen intestinal tube before, during, and after stimulating gallbladder contraction. There were no differences between the two agents in terms of opacification or iodine concentration. Only 19% of the administered dose of either agent was recovered, and the maximum iodine concentration in bile was 10 mg I/ml. The results suggest that this technique has merit for future comparative studies of agents concentrated in the gallbladder.

Administration, Oral↗

PubMedDate not supplied

The ghost of Christmas past: health effects of poverty in London in 1896 and 1991.

OBJECTIVES: To compare the extent to which late 20th century patterns of mortality in London are predicted by contemporary patterns of poverty and by late 19th century patterns of poverty. To test the hypothesis that the pattern of mortality from causes known to be related to deprivation in early life can be better predicted by the distribution of poverty in the late 19th century than by that in the late 20th century. DESIGN: Data from Charles Booth's survey of inner London in 1896 were digitised and matched to contemporary local government wards. Ward level indices of relative poverty were derived from Booth's survey and the 1991 UK census of population. All deaths which took place within the surveyed area between 1991 and 1995 were identified and assigned to contemporary local government wards. Standardised mortality ratios for various causes of death were calculated for each ward for all ages, under age 65, and over age 65. Simple correlation and partial correlation analysis were used to estimate the contribution of the indices of poverty from 1896 and 1991 in predicting ward level mortality ratios in the early 1990s. SETTING: Inner London. RESULTS: For many causes of death in London, measures of deprivation made around 1896 and 1991 both contributed strongly to predicting the current spatial distribution. Contemporary mortality from diseases which are known to be related to deprivation in early life (stomach cancer, stroke, lung cancer) is predicted more strongly by the distribution of poverty in 1896 than that in 1991. In addition, all cause mortality among people aged over 65 was slightly more strongly related to the geography of poverty in the late 19th century than to its contemporary distribution. CONCLUSIONS: Contemporary patterns of some diseases have their roots in the past. The fundamental relation between spatial patterns of social deprivation and spatial patterns of mortality is so robust that a century of change in inner London has failed to disrupt it.

Cause of Death↗

PubMedDate not supplied

In vivo establishment of T98G human glioblastoma.

Human derived T98G glioblastoma has long been utilized as an in vitro model for epidermal growth factor receptor (EGFR)-mediated growth regulation. Recently, T98G has been employed to develop new types of therapy directed at limiting EGFR expression such as by administration of antisense oligonucleotides directed against EGFR encoding mRNA. A major limitation to extending this model for in vivo application is that T98G implanted s.c. or intracerebrally has been reported not to grow in nude mice. In an effort to extend this model to permit in vivo studies, we evaluated the use of Matrigel and orthotopic (intracranial) implantation techniques. When equal volumes of Matrigel were mixed with T98G cell suspensions, tumors developed at both flank and orthotopic locations. Four groups of nude mice were inoculated into the flanks with either 10(5), 10(6), 4 x 10(6) or 10(7) T98G cells in a 150 microliters total volume with Matrigel. In 1/5, 3/5, 1/5 and 1/3 mice receiving 10(5), 10(6), 4 x 10(6) and 10(7) cells, respectively, tumors developed 11, 15, 15 and 15 weeks, respectively, following inoculation. Out of 4 mice inoculated orthotopically (intracranially into the frontal lobe) with only 4 x 10(4) cells and Matrigel, 2 developed tumors. However, all mice (4/4) inoculated orthotopically with 4 x 10(5) cells in a 10 microliters total volume with Matrigel developed tumors. Two were identified histologically following a scheduled sacrifice at 36 and 60 days and two more at 103 and 118 days after sacrifice following abnormal behavior. The best tumor establishment efficacy combined orthotopic implantation of 4 x 10(5) T98G cells with Matrigel. These techniques permit the use of T98G glioblastoma as an in vivo model for new forms of therapy.

Animals↗

PubMedDate not supplied

Traveling to Australia for the Sydney 2000 Olympic and Paralympic Games.

The modern Olympic Games, conducted only once every 4 years since 1900, will be held in Sydney, Australia, from September 15 to October 1, 2000. There will be approximately 35 competition venues, 5 villages, 100 training venues, a media center, and sponsor hospitality areas.1 There will be about 300 events for 28 sports, involving 10,300 athletes from 200 countries, 5,100 team officials, 50,000 volunteers, 15,000 media, a world wide audience of around 3.5 billion viewers and listeners, and up to several hundred-thousand spectators at any one time.1 The Paralympic Games will also be held in Sydney, after the Olympic Games, from October 18 to 29, 2000, with more than 4,000 athletes competing.1 This paper focuses on health and safety issues for travelers to Australia in general, although it makes specific references to advice for visiting Olympic and Paralympic athletes and team staff, who will be traveling to the games. It must be remembered that travel health advice can change, and that travelers should be advised to seek up-to-date travel health advice for Australia closer to their departure.

Australia↗

PubMedDate not supplied

Comparison of three forms of bowel preparations for screening flexible sigmoidoscopy.

Our initial attempts at implementing a high-volume clinic were impeded by the quality of colon preparation. While two Fleet enema preparations were generally easy to administer and well tolerated, the results were frequently poor. Current studies are limited and opinions vary regarding the most effective bowel preparation. The purpose of this study was to compare three forms of bowel preparation for screening flexible sigmoidoscopy. All patients scheduled for screening flexible sigmoidoscopy were given one of three colon preparations: two Fleet enemas; magnesium citrate orally the evening before, clear liquid diet and two Dulcolax suppositories the day of the exam; or magnesium citrate orally the evening before, clear liquid the day of the exam, and two Fleet enemas 1 hour before the procedure. Examinations were performed using a video flexible sigmoidoscopy by specialty trained gastroenterologists. Physicians were asked to rate the preparations as excellent, good, fair, or poor according to explicit criteria, and to document depth of insertion. Results showed that the magnesium citrate and Fleet enema preparation was excellent for 70% of patients, allowing for greater depth of insertion. Need for repeat examination due to poor preparation was infrequent with this group and patient tolerance of the preparation was acceptable.

Analysis of Variance↗