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Biomedical subjects

M Schmidt

Publications and source records attributed to M Schmidt.

At least 973 records · Page 54Linked to original sources
PubMed1975

[Serum bilirubin determination in newborn infants. A new micromethod for the determination of serum of plasma bilirubin in newborn infants].

A micro-method for determination of bilirubin concentration (c) of plasma or serum from newborns was developed. Photometer-readings of the extinction (E) of diluted serum (S) at the bilirubin absorption-band were done against diluted serum as blank, made free from bilirubin by the oxydative power of Chloramin T. Reagents: 1/15 M Na2(HPO4)/K(H2PO4) buffer pH 7,4 (B) and 2 g/dl Chloramin T in B(C). Blank: 0,6 ml C + 0,02 ml S; sample: 0,6 ml B + 0,02 ml S. Reaction time: 4--8 min; filter Hg 436 nm; 1 cm light-path. c (mg/dl) = E X 43,5. Accuracy was determined by a calibration curve (x: mg/dl c of diazo-method; ): E of micro-method); b = 1,008. Reproducibility in series at 14,2 mg/dl resp. 47,9 mg/dl was 1,5% resp. 1,6%. Linearity between E and c is good up to 48 mg/dl c. Specifity was only reduced by serum hemoglobin (h). Sodium heparinate (50 E./ml S) and carotin (952 microgramm/1) had no effect upon E; plasma anticoagulated with heparinate therefore can be used instead of serum. Concentration of h is without importance for clinical purposes; 100 mg/dl h causes about 0,2 mg/dl wrong depression of c.

Bilirubin↗
PubMed1975

Effect of dopamine on renin secretion in the anesthetized dog.

Intrarenal perfusion of dopamine (6 mug/kg/min for 10 min) caused a significant increase of renin secretion, together with a significant increase in renal blood flow. This renin hypersecretion is not accompanied by any significant alteration in renal perfusion pressure, kalemia or natriuresis. The role of intrarenal dopaminergic receptors has been studied: (a) Haloperidol (intrarenal perfusion of 50 mug/kg/min for 20 min) suppresses the renal vasodilation and renin hypersecretion induced by dopamine. (b) Propranolol (intrarenal perfusion of 1 mg/kg in 15 min, then of 4 mg/kg/hr) alters neither the renal vasodilation nor the renin hypersecretion induced by dopamine. These observations support the assumption that the dopaminergic receptors are brought into play in the two renal responses to dopamine studied by us.

Anesthesia↗
PubMed1975

beta-blocking drugs and renin secretion in the anaesthetized dog.

1. The effect of propranolol and sotalol on renin secretion was studied in the anaesthetized dog. 2. beta-adrenergic blockade did not modify basal renin secretion and did not affect the rise of renin secretion induced by haemorrhage or aortic constriction. 3. beta-adrenergic blockade diminished the rise in renin secretion induced by stimulation of the renal nerves.

Animals↗
PubMed1975

Transplantation of human cancers to nude mice and effects of thymus grafts.

Human cancers were transplanted to nude mice; a cell line originating in the foregut (distal stomach or first portion of the duodenum) produced exponential nonmetastasizing growth, preventable by prior thymus implantation. Implantation of thymuses into mice with established tumors resulted in increased animal survival and in four instances in complete regression of the neoplasm. Cell lines of pancreatic carcinoma and melanoma and surgical specimens of melanoma and of neoplasms from the pancreas and colon showed variable growth patterns in this system. Repeated attempts to transplant gastric adenocarcinoma were unsuccessful.

Adenocarcinoma↗
PubMed1975

Do elephants ever forget?

Three adult female elephants (Elephas maximus) were tested on a light-dark discrimination problem with an 8-yr intertrial interval. The first subject took only 6 min to reach criterion and made only two errors, suggesting remarkable retention. The other two subjects were found to have visual anomalies that would have gone undetected without this research.

Animals↗
PubMed1975

[A device for safe handling of vacuum-dried microbes (author's transl)].

By analogous application of the way of packing which is common for the transportation of infectious material (glass tubes in protective covers) in the handling of vaccuum-dried microbes (ampoule within the ampoule) the risk encountered when opening such receptacles may be reduced to an almost complete absence. Technical details are explained by a schematic representation. The method is suitable for the preservation of freeze-dried vaccines. All processes involved in the practical performance of the method which is ready for routine application are considered in detailed instructions for use.

Bacteria↗
PubMed1975

Proteinuria as diagnostic marker after human kidney transplantation.

Molecular weight analyses of urinary proteins in 34 patients following cadaveric kidney transplantation were performed by SDS-PAA-electrophoresis in order to diagnose transplant complications. A micromolecular 'tubular' proteinuria (mw 70-10,000) was found in all post-operative urines. Later on during clinically normal periods the patients exhibited an unphysiological proteinuria of mw 70-40,000. Recurrence of tubular proteinuria was associated with rejection episodes and acute kidney failure. Twelve patients developed a macromolecular glomerular proteinuria (mw greater than 60,000), caused by recurrent glomerulonephritis, glomerular rejection disease or renal vein thrombosis. Steroid treatment reduced the glomerular permeability for macromolecules above mw 65,000.

Acute Disease↗
PubMed1975

[Optimal preservation of function in vacuum-dried bacteria (author's transl)].

Experience has shown that there are natural processes assuring a good preservation of virulence of delicate bacteria over intervals between epidemic outbreaks. Similarly, fetal bovine serum enriched with 10% saccharose was found to be an ideal medium for rendering bacteria well preservable without losing essential functional characteristics by means of vacuum-drying. Cotton wool DAB 7 has proved to be a good support. The available findings would let also delicate aerobic and anaerobic bacteria appear to be suitable for this kind of preservation, retaining their full capacity to multiply for at least one year inclusive of sensitive functional parameters. Results have been grouped in a number of synoptic tables, Bacteria that would not admit of the method of preservation described, have not been found thus far.

Aerobiosis↗
PubMed1975

[Effect of d-1 propranolol on urinary osmolarity after furosemide in anesthesized dog].

The effects of propranolol (l mg/kg/H infused in the renal artery) on the diuretic action of furosemide (20 mg/kg i.v.) have been studied in pentobarbital anesthetized dogs. We obtained the 3 following results : the urine remained isotonic to the plasma during the 6 hours following the furosemide injection ; the urinary output of sodium and water, measured during 6 hours after furosemide injection, was increased ; the renin hypersecretion was inhibited.

Animals↗