Detection of chiral recognition in various intermolecular interaction systems using mass spectrometry has become important for the modern fields of analytical chemistry, organic chemistry, and biochemistry due to the characteristic nature of the rapid method and the trace amount needed. This review presents the various methods for detecting and evaluating chiral recognition used primarily in fast atom bombardment mass spectrometry. Emphasis is put on fundamentals and applications of these methods for variously existing enantioselective intermolecular interaction systems.

Anti-Bacterial Agents↗

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Portosystemic shunt with polysplenia and hypoplastic left heart syndrome.

We report a unique case of portosystemic shunt with polysplenia and hypoplastic left heart syndrome. The abnormal vessel connected the right renal vein and the splenic vein. The shunt flow was from the systemic vein to the portal vein before the Fontan-type operation, but it reversed and ran from portal vein to systemic vein after the Fontan-type operation.

Abnormalities, Multiple↗

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The effects of UV radiation and antioxidants on life span and lipid peroxidation in the rotifer Asplanchna brightwelli.

Lipid peroxidation (LP) rates and life span were determined in control and in ulraviolet (UV) irradiated rotifers of the species Asplanchna brightwelli. UV irradiation administered at 6 h of life significantly increased the rate of LP as the rotifers aged; it also produced a significant shortening of rotifer life span. Pretreatment with 20 microg/ml of vitamin E blocked the increase in LP and the decrease in life span induced by UV radiation. The antioxidant synergist 3,3'-thiodipropionic acid had no such UV-protective effect. These results indicate that the decrease in life span caused by UV radiation may be mediated through the production of lipid peroxides.

Journal Article↗

PubMedDate not supplied

Effects of dimethyldiethanolammonium on the ionic permeability of chemically modulated channels in the frog endplate and the Onchidium neuron.

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Animals↗

PubMedDate not supplied

Alterations in free radical activity in aging Drosophila.

Drosophila were examined to see if there is an increase in damage associated with free radical activity in older flies. The levels of superoxide radical and of lipid peroxides were higher in membrane samples from older flies. Also, in older Drosophila there was a significant decrease in membrane fluidity, as determined by fluorescence polarization, and an increase in ATP-dependent calcium uptake. In homogenates from whole flies, the concentration of inorganic peroxides and activity of the proteolytic enzyme, cathepsin B, increased with age. During their lifetime, vestigial wing Drosophila displayed a greater level of free radical activity than wild-type flies and a significantly shorter life span (26.7 +/- 0.7 days vs. 34.4 +/- 1.0, p < 0.01). These results indicate that the level of oxidative stress is closely coupled to cellular damage and to life span, and they indicate that free radicals may play a central role in the aging process in Drosophila.

Aging↗

PubMedDate not supplied

Cytokine network in the central nervous system and its roles in growth and differentiation of glial and neuronal cells.

Cells resident within the central nervous system (CNS) can synthesize, secrete and respond to inflammatory cytokines not only contributing to the responses to injury or immunological challenge within the CNS, but also regulating their own growth and differentiation potential. The actions and cell communication via cytokines in the CNS are designated as the CNS cytokine network, in which microglia and astrocytes play the central roles. To further characterize the CNS cytokine network we investigated the differences in roles of these cells, and found that microglia might contribute to the early phase of cytokine production reaction and that astrocytes might contribute the late phase of the reaction. We also investigated roles of inhibitory cytokines such as TGF beta, IL-4, and IL-10, and showed that each might play a distinct role in the inhibitory regulation in the CNS. We summarized our previous report about cellular distribution of cytokine receptors in the CNS cells and discussed their roles in the CNS cytokine network. Finally, we investigated that expression of IL-6 and IL-2 receptors in neuronal and oligodendrocytic differentiation, respectively. From these results, we discussed the features of the CNS cytokine network.

Animals↗

PubMedDate not supplied

Glomerulocystic kidney in a domestic dog.

Glomerulocystic kidney was diagnosed in a 5-year-old female Shiba dog, which died from chronic renal failure with convulsions, vomiting and diarrhoea. Haematological examination revealed non-regenerating anaemia, azotaemia and high serum creatinine. Grossly, both kidneys were mildly atrophic with multiple small cysts in the cortex. Histopathological examination revealed marked dilatation of Bowman's space, often with glomerular atrophy or loss, and mild interstitial fibrosis. Bowman's basement membranes (BMs) were tortuous and thickened, with patchy calcification. Glomerulo-tubular junctions in the urinary pole side of the kidneys had a stenotic appearance associated with thickening of Bowman's BMs and calcification. Focal interstitial fibrosis around the glomerulo-tubular junction was also found. Continuity with the proximal tubule was evident in cystic glomeruli. Ultrastructurally, marked thickening of Bowman's BMs with many granular deposits in the urinary pole side was observed. The findings indicate that glomerular cystic changes may have developed as a consequence of glomerulo-tubular junctional stenosis due to thickened Bowman's BMs and focal periglomerular fibrosis in the urinary pole side of the kidneys.

Animals↗