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Biomedical subjects

M S Fox

Publications and source records attributed to M S Fox.

At least 73 records · Page 4Linked to original sources
PubMed1973

Marker discrimination in transformation and mutation of pneumococcus.

Earlier investigations of pneumococcal transformation revealed a function (hex(+)) responsible for severely reducing the transformation yield of certain markers. A mutational alteration (hex(-)) responsible for the loss of this function has been transferred into a hex(+) strain to permit a comparison of the hex(+) and hex(-) phenotypes in an isogenic background. The loss of the hex(+) function results both in a loss of the capacity to eliminate the low efficiency markers in transformation and a substantial increase in the spontaneous mutation rate. These properties of the hex(-) strain could result from the loss of a capacity to eliminate certain classes of mismatched base pairs that occur as intermediates in both transformation and mutagenesis.

Base Sequence↗
PubMed1971

Bacterial inactivation of transforming deoxyribonucleate.

Competent and noncompetent bacteria are able to bind deoxyribonucleate (DNA). The consequence of this binding is different for the two types of bacteria. Competent bacteria are able to utilize the DNA for transformation. DNA exposed to noncompetent bacteria loses its biological activity with a coincident reduction in the double-stranded molecular weight. The reduction in molecular weight does not appear to be sufficient to account for the loss of biological activity observed.

Bacillus subtilis↗
PubMed1971

Fate of transforming deoxyribonucleate in Bacillus subtilis.

The majority of donor deoxyribonucleate (DNA) at early stages after uptake was found in a complex with a cell component which changes its buoyant behavior on equilibrium density gradients. Analysis of the recipient cell lysates, after treatment to dissociate the complex, showed about two-thirds of the donor molecules in denatured form and the rest associated with recipient DNA. Incubation of cells after DNA uptake leads to the disappearance of denatured donor DNA and to the increase of donor label associated with recipient DNA. Some characteristics of a component from intact cells or spheroplasts with affinity for denatured Bacillus subtilis DNA are described.

Bacillus subtilis↗