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Biomedical subjects

M Reiss

Publications and source records attributed to M Reiss.

At least 109 records · Page 6Linked to original sources

Some psychological aspects of tinnitus.

Tinnitus rarely can be cured. The patient, however, needs help to avoid countless ineffective treatments and considerable cost. The clinical examination of patients with severe and chronic tinnitus must include associated psychological disturbances. After all medical and audiological treatments have failed, tinnitus patients often are advised to "learn to live with it." The aim of psychosocial treatment is assisting patients in the identification of aggravating factors in tinnitus and teaching them various coping skills.

Adaptation, Psychological↗

[Significance of CD-ROM in medicine].

The article evaluates some multimedia programs offer for computers for medicine information, teaching and learning. Information technology has the potential to revolutionize the way medicine is learned by students and practitioners. Medicine with its abundance of facts and richness of imaging techniques has always been adopting new technologies to improve teaching and training. As a natural consequence, the use of computer-based multimedia technology is generally increasing in medicine. Especially the use of interactive multimedia technology seems promising to improve training and education and increase accessability of highly specific material. It can combine the best of the text-book and the video, but above all the use of interaction should produce untold benefits.

CD-ROM↗

Genetic associations between lateral signs.

Only a few authors examined several lateral asymmetries in families simultaneously. Seven lateralities (handedness, footedness, eyedness, earedness, hand clasping, arm folding and leg crossing) were examined in a sample of 292 biologically related parent-offspring triads and 36 sibling pairs. Analysis of the family data showed a significant association between parents and children in most lateralities. The frequency of left-sidedness increased with the number of left-sided parents. Only in the case of footedness and hand clasping there are no significant correlations. Additionally we investigated the inter-relationship between the lateralities in the parental generation. There is a relative strong relation especially among the functional asymmetries. The study analysed also the genetic association (linkage) according to McManus & Mascie-Taylor (1979) between seven different lateralized characteristics. It is shown that the lateral asymmetries correlate genetically relatively imperfectly with each other comparing the inter-relationship between the lateralities in the parental generation. This implicates a multigenetical and also multifactorial determination of laterality in human being.

Adult↗

[Hand clasping--an overview].

The literature concerning the asymmetry clasping hands is reviewed based on 192 studies. This paper describes the incidence, sex differences, age differences and genetical problems including twinning. The incidence of left hand clasping ranges from 30% to 75% (mean 43%). The review confirms the so-called east-west-gradient and there is a predominance of the left type in Europe. Age and sex differences are only small. There is only a small relationship between hand clasping and handedness. 18 authors examined hand clasping in families and 4 in twins. The family data suggest that hand clasping may be under genetic control, yet it is clear that no simple genetic model for the inheritance can be applied. Both monozygotic and dizygotic twins show a low concordance and the R-R, R-L and L-L pairs in monozygotic and dizygotic twins are in binomial distribution.

Functional Laterality↗

[Caustic burns of the esophagus in childhood].

Acute burns of the upper gastrointestinal tract require an immediate diagnostic and therapeutic approach. Early endoscopy has been shown to assist greatly in determining the further treatment of corrosive injury. The main task of radiology is to detect perforations. Early corticosteroid treatment seems to be effective in preventing stricture development in second degree burns.

Adrenal Cortex Hormones↗

Amperometric phenol biosensor based on a thermostable phenol hydroxylase.

Phenol hydroxylase (EC 1.14.13.7) was produced using Bacillus stearothermophilus in a 5-1 batch fermentation leading to approximately 17 units after 6 h. The partially purified phenol hydroxylase was entrapped in a sol-gel matrix. The enzyme-loaded silica gel was attached to the sensitive top of a Clark-type oxygen electrode and its application as a phenol biosensor was tested. There was linearity between the maximal rate of oxygen consumption and phenol concentration in the range between 2.5 and 400 microM at 40 degrees C and pH 7.6. The signal could be read off after 10 s at a concentration of 400 microM phenol. The sensor lost 20% of its activity within 7 days. Para-substituted phenols were not detectable.

Biosensing Techniques↗

Determination of BOD-values of starch-containing waste water by a BOD-biosensor.

The control of waste water plants is difficult or even impossible using the classical determination method for biological oxygen demand (BOD), because of its high time consumption of five days. A determination within some minutes is possible by microbial BOD-sensors. However, high molecular weight substances cannot be detected, a problem which can be overcome by the use of additional enzymes. For the application in a flow-through system to analyse starch containing waste water, alpha-amylase and amyloglucosidase were immobilized by adsorption to polystyrene or polypropylene carriers followed by crosslinking. Furthermore, covalent coupling to different nylon carriers, derivatives of chitin, silanized glass beads and silanized beads of foamed glass was tried. Chitin and Lewatit were the best suited carriers for the immobilization of alpha-amylase and amyloglucosidase. Two glass columns were filled with the immobilized enzymes and inserted into a commercial BOD-sensor containing the yeast Trichosporon cutaneum as biological component. The system was stable for more than two months under storage and one month under working conditions. A comparison of different starch types resulted in a hydrolysis of more than 80% in case of potato starch whereas grain starch was hydrolized only for 40-50%. Sensor-determined BOD-values of waste water with potato starch were nearly identical with BOD5-values resulting from the classical method.

Biosensing Techniques↗

Transforming growth factor beta type I receptor kinase mutant associated with metastatic breast cancer.

Malignant breast carcinoma cell lines are frequently refractory to transforming growth factor beta (TGF-beta)-mediated cell cycle arrest. To identify molecular mechanisms of TGF-beta resistance, we have conducted a comprehensive structural analysis of the TGF-beta receptor types I (TbetaR-I) and II (TbetaR-II) genes in primary human breast carcinomas and associated axillary lymph node metastases. No evidence for loss of expression (n=14) or structural alterations of the TbetaR-II gene (n=30) were identified. However, 2 of 31 primary carcinomas and 5 of 12 lymph node metastases carried a C to A transversion mutation resulting in a serine to tyrosine substitution at codon 387 (S387Y) of the TbetaR-I receptor gene. This TbetaR-I mutant has a diminished ability to mediate TGF-beta-dependent effects on gene expression as compared with wild-type TbetaR-I. S387Y is the first reported mutation in the TbetaR-I gene in human cancer that was primarily associated with lymph node metastases in the present series.

Amino Acid Sequence↗

[Cogan syndrome--a case report].

We present a case report of Cogan's syndrome and a review of the literature. Cogan's syndrome, described in 1945, consists of nonsyphilitic interstitial keratitis accompanied by rapidly progressive bilateral hearing loss, tinnitus, and vertigo. 40% develop bilateral complete deafness and 70% show signs of systemic disease mostly in the form of cardiovascular symptoms. The mortality rate of 10% is mainly due to vasculitis. The disease is caused by an autoimmune response. One male patient had a typical interstitial keratitis and bilateral dramatically progressive deafness combined with failure of the vestibule. Corticosteroids and immunosuppressants are the treatment of choice. The importance of being aware of audiotory-vestibular dysfunction occurring in patients with ocular inflammation and the role of early immunotherapy in preventing deafness has to be emphasised.

Autoimmune Diseases↗

[Gunshot injuries in the head-neck area--basic principles, diagnosis and management].

Bullet wounds are a rare occurrence during times of peace. Recently, however, there has been a general increase in the number and severity of this type of trauma in our case load. First, the possible firearms and the individual types of ammunition will be discussed. Based on this background, the possible types of wounds are presented. Principally, one distinguishes ricocheting shots from grazing ones, and those leaving bullets lodged in the body from those with perforating wounds. The extent of tissue damage depends on internal lacerations, on the compression of the tissue and on the temporary cavitation along the projectile path. In contrast to other types of injuries, which are caused by a blow or impact to the face or skull, gunshot traumas are characterized by an irregular path, as well as, by localized destruction of bones with associated effects. In this connection, the severity of the bullet wound also depends upon the extent of involvement of the viscerocranium. As causes of gunshot wounds during times of peace, suicidal intent, the negligent handling of firearms and especially brutal crimes are those which come into consideration first and foremost. The diagnostic aspect of firearm wounds, beside anamnesis, comprises comprehensive X-ray diagnostics for a complete picture. From the therapeutic side, tetanus serum and antibiotics as a prophylactic are recommended initially. The operative treatment should take place depending on the injury with the removal of a possible projectile. Bullet wounds always require an interdisciplinary approach to treatment.

Causality↗

Functional characterization of transforming growth factor beta type II receptor mutants in human cancer.

We recently identified missense mutations at amino acid residues 526 and 537 located within the highly conserved subdomain XI of the transforming growth factor beta type II receptor (TbetaR-II) serine-threonine kinase in two human squamous carcinoma cell lines. These cell lines are resistant to transforming growth factor beta-mediated inhibition of growth. Moreover, treatment with transforming growth factor beta fails to increase the levels of type 1 plasminogen activator inhibitor and fibronectin synthesis. To test the effects of the mutations on receptor function, mutant TbetaR-II cDNAs were expressed in TbetaR-II-deficient T47D cells. Cyclin A promoter activity was reduced by 50% in cells expressing wild-type TbetaR-II but increased 2-fold in cells transfected with either of the two mutant receptors. Conversely, plasminogen activator inhibitor type 1 promoter activity was increased 6-fold in cells transfected with wild-type receptor but not with either of the two mutant receptors. Moreover, the activity of both mutant serine-threonine kinases was strongly reduced compared to that of the wild-type receptor. Thus, the amino acid residues at positions 526 and 537 seem to be essential for kinase function and signaling activity of the TbetaR-II.

Carcinoma, Squamous Cell↗

[Dysphagia as a symptom of myxedema].

We present a 79 year old female patient with dysphagia since two years. She showed also a lack of initiation and dysphonia. In the region of the larynx an edema was found and the tongue was hypertrophied. The X-ray examination demonstrated a dilated esophagus without impairment of the passage way. Esophagoscopy showed also important edema in the hypopharynx and the entry into the esophagus. The TSH-0 was 74.45 mmu/l, the T3 0.23 and the T4 was 24 nmol/l. Scintigraphically an only cherry-stone small region with active thyroid tissue was revealed. Severe hypothyroidism responsible for secondary dysphagia was diagnosed. L-Thyroxin was administered (150 micrograms/d). There was a dramatical improvement. The signs of edema and the dysphagia decreased. The examination a half year later demonstrated a patient without any dysphagia or edema. The symptom dysphagia is defined and an interdisciplinary approach emphasized.

Aged↗

[Anaphylactic shock. Diagnosis-therapy--emergency measures].

Anaphylaxis is an immediate, life-threatening, general allergic reaction mediated by bioactive substances released by mast cells. Symptoms include diffuse urticaria, gastrointestinal disorders (sensation of fullness, diarrhea and abdominal cramps), bronchospasm, edema, airway obstruction, hypotension, cardiovascular collapse, and even death. The present review discusses the adequate diagnostic and therapeutic management based on our own experience.

Anaphylaxis↗