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Biomedical subjects

M Reinert

Publications and source records attributed to M Reinert.

At least 37 records · Page 2Linked to original sources
PubMed1995

Amino acid sequence of HSP-1, a major protein of stallion seminal plasma: effect of glycosylation on its heparin- and gelatin-binding capabilities.

We report the complete amino acid sequence of HSP-1, a major protein isolated from stallion seminal plasma or acid extracts of ejaculated spermatozoa. The protein consists of 121 amino acids organized in two types of homologous repeats arranged in the pattern AA'BB'. Each of the 13-15-residue A-type repeats contains two O-linked oligosaccharide chains. The B-type repeats span 44-47 amino acids each, are not glycosylated, and have the consensus pattern of the gelatin-binding fibronectin type-II module. This domain also occurs in the major bovine seminal plasma heparin-binding proteins PDC-109 (BSP-A1/A2) and BSP-A3. However, unlike the bovine proteins which bind quantitatively to a heparin-Sepharose column, stallion HSP-1 was recovered in both the flow-through and the heparin-bound fractions. Structural analysis showed that the two HSP-1 forms contain identical polypeptide chains which are differently glycosylated. Moreover, size-exclusion chromatography showed that heparin-bound HSP-1 associates with HSP-2, another major seminal plasma protein, into a 90 kDa product, whereas the non-heparin-bound glycoform of HSP-1 is eluted as a monomeric (14 kDa) protein. This suggests that glycosylation may have an indirect effect on the heparin-binding ability of HSP-1 through modulation of its aggregation state. On the other hand, both glycoforms of HSP-1 displayed gelatin-binding activity, indicating that the molecular determinants for binding heparin and gelatin are different.

Amino Acid Sequence↗
PubMed1991

[Indications and use of fissure sealants in public dental health care in Denmark. A questionnaire-investigation].

During the past decade usage of the fissure sealant technique for occlusal caries prevention has been increasingly recommended. This study explores variations in indications and usage of this technique in Denmark. A questionnaire was sent to 205 chief dental officers in the Danish Public Child Dental Health Service (PDHS) covering 90% of Danish children and to municipalities, where dental health to children is provided by private practitioners. More than half of the respondents did not use firmly defined criteria for fissure sealant application. Oral hygiene and previous caries experience were most often stated as indications. Actual usage of the sealant technique differed significantly from the PDHS and private clinics. Thus, 33% of the chief dental officers in the PDHS said that sealants were routinely applied to 8- and 13-year-olds, 43% to 30-80% of the children, and 15% used sealants to less than 10% of 8- and 13-year-olds. In contrast, only 5% of the private clinics reported routinely use of sealing technique, 22% used application to 30-80% of the children, while more than one third of the private practitioners used sealants to less than 10% of the 8- and 13-year-olds. In spite of the significant difference in sealant usage between PDHS and private practitioners, it was not possible to see a corresponding difference neither in caries prevalence nor in occlusal filling incidence. Moreover, analysis of PDHS caries prevalence data revealed that PDHS variations could not be explained by variations of risk in terms of social classes and caries incidence.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1990

[What does the asthmatic patient know about his disease?].

Asthmatics attending two specialist offices were interviewed to determine how well informed they were. Fifty per cent of the patients did not know what it was they were suffering from; 50% had no idea about the pathomechanism of bronchial asthma; 75% believed that bronchial asthma was curable; 50% believed that treatment as required sufficed. Two-third feared dangerous adverse effects of the drugs they were taking. Topical steroids were largely unknown. Patients who had been in our care for 1 to 2 years were already much better informed than new patients. The knowledge of the latter was appreciably improved by a single oral session of instruction, and the reading of a self-written brochure. The main thrust of this educational effort must be to familiarize the asthmatic patient with the following points: Asthma is a chronic disease and must be treated regularly and life-long. The drugs employed have no injurious adverse effects. It is necessary to repeat such information sessions at short intervals and to monitor their effectiveness.

Adult↗
PubMed1990

[Case distribution in a pneumonologic practice].

Among the diseases seen in the lung specialist's office, affectations of the upper (32.8% and lower (49.6%) airways are by far and away the most common. The allergic forms predominate, probably on account of the high prevalence of pollen allergy. Internistic diseases account for 12.8%, and disorders from the fields of paediatrics, ENT medicine and dermatology (3.5% urticaria) also make a non-inconsiderable contribution. Diseases of the lungs are not very common, but are highly multifarious. In addition to the above-mentioned disorders, paediatric and geriatric problems are quite common: children up to the age of 15 years account for about 20%, and patients older than 60 years for 10% of the total number of patients.

Adolescent↗
PubMed1987

[Treatment of an asthmatic child becomes more effective when the mother is well informed, auscultates her child and keeps a diary].

The asthmatic child himself can do nothing against his illness. It is the task of the mother, assisted by the physician, to prevent the child suffering from his asthma. To achieve this purpose it is not sufficient to prescribe medicaments only. But a comprehensive information of the mother about asthma bronchiale, the auscultation of the child by his mother and a daily record of the symptoms make the medicaments effective.

Asthma↗
PubMed1983

Attitudes of French adolescents toward sexuality.

Three hundred and eighty-six French adolescents from 16 to 18 years of age (145 boys and 241 girls) were questioned about sexuality. The five questions used covered their opinions on: abortion, masturbation, sexual intercourse during adolescence, homosexuality and marriage. The comparison of their responses to the five questions was expected to bring out the differences in their reactions in these areas. In fact, the results revealed that the subjects were not concerned to the same degree by the five questions. It was also observed that few adolescents were completely "liberal" and even fewer completely "conservative". The importance of socio-cultural factors, especially religion, associated with the attitudes of the subjects appeared undeniable. Some interesting differences in attitudes expressed by the two sexes were brought out.

Abortion, Induced↗
PubMed1981

When is a positive nasal challenge test clinically relevant?

339 subjects with a clinically manifest or latent allergy to different allergens were tested intranasally: 163 with grass pollens, 19 with tree pollens, 46 with cat fur, 23 with dog hair, 79 with mites and 9 with horse hair. Two thirds of the patients with a latent allergy towards pollens either reacted to a pollen concentration of 1% w/v or not at all, one third responded to 0.1% w/v. Two thirds of the pollinosis patients reacted at 0.01% w/v, one third at 0.1% w/v. Threshold dosages were also found for animal fur: The limits are at 1.5% w/v (manifest allergy) and 150% w/v (latent allergy) and the transitional range at 15% w/v. No threshold was found for mites. The study shows that for certain allergens there exist threshold concentrations which should not be exceeded. Where there is no threshold dose, as e.g. with mites, the nasal test is no more informative than the skin test.

Adolescent↗